Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,715,979 A→G 100% F8L (TTC→CTC)  amaB ← N‑carbamoyl‑L‑amino acid amidohydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,715,9790A→G100.0% 865.5 231F8L (TTC→CTC) amaBN‑carbamoyl‑L‑amino acid amidohydrolase
Reads supporting (aligned to +/- strand):  new base (100/130):  ref base (0/0):  total (100/131)

Only 100 of 231 total aligned reads displayed.

ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076
                                                                                                  |                                                                                                 
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  1:177600/1‑101 (MQ=255)
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  1:2740182/1‑101 (MQ=255)
 tcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtt                                                                                                <  2:2237233/101‑1 (MQ=255)
  cggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGttt                                                                                               <  2:1003575/101‑1 (MQ=255)
  cggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGttt                                                                                               >  1:3000821/1‑101 (MQ=255)
   ggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtttt                                                                                              <  2:2390310/101‑1 (MQ=255)
     tCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtct                                                                                            >  2:869562/1‑101 (MQ=255)
     tCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtct                                                                                            >  2:1893950/1‑101 (MQ=255)
       aCGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTcc                                                                                          <  2:1127566/101‑1 (MQ=255)
        cGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCg                                                                                         >  1:3037901/1‑101 (MQ=255)
        cGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCg                                                                                         <  1:4233037/101‑1 (MQ=255)
           gcTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGt                                                                                      <  2:2694942/100‑1 (MQ=255)
            cTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTg                                                                                     <  2:343884/101‑1 (MQ=255)
                cGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATgg                                                                                 <  1:4065482/101‑1 (MQ=255)
                 ggTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGc                                                                                <  1:1421530/101‑1 (MQ=255)
                 ggTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGc                                                                                >  2:1786251/1‑101 (MQ=255)
                   ttgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCAt                                                                              <  1:4154610/101‑1 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATc                                                                             >  2:2968982/1‑101 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATc                                                                             >  1:137983/1‑101 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            <  1:680129/101‑1 (MQ=255)
                      ttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGCTGGcctatg                                                                           >  2:623267/1‑96 (MQ=255)
                      ttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTg                                                                           <  2:405830/101‑1 (MQ=255)
                      ttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTg                                                                           <  2:2637667/101‑1 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          <  2:1477237/101‑1 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGGTTTCTCCGGGTGATGGCATCTgg                                                                          >  2:1677980/1‑101 (MQ=255)
                         ccccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCt                                                                        >  1:1319274/1‑101 (MQ=255)
                          cccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTg                                                                       >  2:3817554/1‑101 (MQ=255)
                             ccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtct                                                                    >  1:3408450/1‑101 (MQ=255)
                             ccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtct                                                                    <  2:78472/101‑1 (MQ=255)
                              cgcgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtctc                                                                   <  1:833843/101‑1 (MQ=255)
                              cgcgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtctc                                                                   <  1:1564034/101‑1 (MQ=255)
                                  gtCCCCGGCCCGACGTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTg                                                               <  1:1663019/100‑1 (MQ=255)
                                  gtCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTg                                                               <  2:1591651/100‑1 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              <  1:1309624/101‑1 (MQ=255)
                                    ccccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtc                                                             >  1:45239/1‑101 (MQ=255)
                                     cccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcg                                                            >  1:1507804/1‑101 (MQ=255)
                                      ccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcgt                                                           <  1:869647/101‑1 (MQ=255)
                                      ccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcgt                                                           >  1:1070308/1‑101 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         >  1:1921170/1‑101 (MQ=255)
                                         gCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTg                                                        <  2:3896903/101‑1 (MQ=255)
                                          cccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGc                                                       >  2:2755853/1‑101 (MQ=255)
                                           ccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCg                                                      >  1:3554203/1‑101 (MQ=255)
                                             gACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCggg                                                    <  1:1616922/101‑1 (MQ=255)
                                                 tGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCtt                                                <  1:1249268/101‑1 (MQ=255)
                                                  gcgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCttt                                               <  1:3799593/101‑1 (MQ=255)
                                                   ctccATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTg                                              <  2:907621/99‑1 (MQ=255)
                                                     ccATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGcc                                            <  2:1386501/101‑1 (MQ=255)
                                                     ccATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGcc                                            >  2:20293/1‑101 (MQ=255)
                                                     ccATCTGAATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGcc                                            >  2:2485521/1‑101 (MQ=255)
                                                          tGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCtt                                       <  1:1894074/101‑1 (MQ=255)
                                                          tGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCtt                                       <  2:3777705/101‑1 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  2:574948/101‑1 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  1:1006797/101‑1 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      >  1:3945133/1‑101 (MQ=255)
                                                            cATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGc                                     <  2:1995641/101‑1 (MQ=255)
                                                             aTGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCg                                    >  1:3241161/1‑101 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  >  1:1538304/1‑101 (MQ=255)
                                                                aGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACt                                 <  2:2805788/101‑1 (MQ=255)
                                                                 ggCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTc                                <  2:4422929/101‑1 (MQ=255)
                                                                 ggCTGTCCCACAGGCGCGCGGAATTGATACGGACGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTc                                <  1:3817807/101‑1 (MQ=255)
                                                                   cTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAt                              <  1:1998348/101‑1 (MQ=255)
                                                                   cTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAt                              <  2:3411746/101‑1 (MQ=255)
                                                                   cTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAt                              <  2:3044571/101‑1 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             >  2:3011027/1‑101 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            <  1:2322200/101‑1 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            <  1:3894642/101‑1 (MQ=255)
                                                                      tCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtttt                           <  2:1535277/101‑1 (MQ=255)
                                                                          acaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGa                       >  2:2107462/1‑101 (MQ=255)
                                                                          acaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGa                       <  1:1120452/101‑1 (MQ=255)
                                                                          acaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGa                       <  2:2574448/101‑1 (MQ=255)
                                                                          acaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGa                       >  1:3434462/1‑101 (MQ=255)
                                                                           caGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAc                      >  1:3660262/1‑101 (MQ=255)
                                                                           caGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAc                      >  2:3342537/1‑101 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCGTCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     >  1:398496/1‑101 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCTTTTTCTGAcc                     >  1:1860522/1‑101 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGCGATGGCATCTGGCTGTCTCCCTGTCGCCTGCGGGTGTTTGCCCTCTTGCGACTCATTTTccgccc                     >  1:1691858/1‑96 (MQ=255)
                                                                             ggCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCg                    <  1:3630622/101‑1 (MQ=255)
                                                                               cgcgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGAt                  <  2:4244346/101‑1 (MQ=255)
                                                                                gcgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGAtt                 <  1:371886/101‑1 (MQ=255)
                                                                                gcgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGAtt                 >  2:1846225/1‑101 (MQ=255)
                                                                                gcgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGAtt                 <  1:2473629/101‑1 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               >  2:1351363/1‑101 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               <  1:3122651/101‑1 (MQ=255)
                                                                                   cgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGt              >  2:1961728/1‑101 (MQ=255)
                                                                                      aaTGGATCCGGAGGTTTTCTCCGGGTGATGGCATCTGGCGGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCag           >  2:3666620/1‑101 (MQ=255)
                                                                                        ttGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCagag         <  2:3567384/101‑1 (MQ=255)
                                                                                        ttGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCagag         >  2:226255/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        >  2:3594665/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        >  1:2144313/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        <  2:3949420/101‑1 (MQ=255)
                                                                                          gATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGa       >  1:1792038/1‑101 (MQ=255)
                                                                                          gATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGa       >  1:2210929/1‑101 (MQ=255)
                                                                                          gATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGa       >  1:2408815/1‑101 (MQ=255)
                                                                                           aTACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaa      <  1:2693004/101‑1 (MQ=255)
                                                                                            tACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaaa     >  2:4174873/1‑101 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    >  1:756833/1‑101 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    >  2:4002362/1‑101 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    <  1:218256/101‑1 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   >  1:3424692/1‑101 (MQ=255)
                                                                                               ggAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAagag  <  1:2794180/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG