Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,731,802 G→C 100% P509A (CCC→GCC)  RCAP_rcc02540 ← diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,731,8020G→C100.0% 793.7 211P509A (CCC→GCC) RCAP_rcc02540diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (122/89):  ref base (0/0):  total (122/89)

Only 100 of 222 total aligned reads displayed.

CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGG  >  NC_014034/2731705‑2731894
                                                                                                 |                                                                                            
cAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACGGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcg                                                                                           >  1:1440974/1‑101 (MQ=255)
cAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcg                                                                                           >  2:1631418/1‑101 (MQ=255)
 aGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgc                                                                                          >  1:4246174/1‑101 (MQ=255)
 aGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgc                                                                                          >  1:286130/1‑101 (MQ=255)
 aGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgc                                                                                          >  1:1522114/1‑101 (MQ=255)
     cACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgc                                                                                      >  1:292525/1‑101 (MQ=255)
     cACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgc                                                                                      <  1:867529/101‑1 (MQ=255)
        ccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcccccgcgcc                                                                                   >  1:3689311/1‑100 (MQ=255)
        ccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCa                                                                                   <  2:1364335/101‑1 (MQ=255)
        ccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCa                                                                                   >  2:743796/1‑101 (MQ=255)
          ggTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGc                                                                                 >  1:1221984/1‑101 (MQ=255)
           gTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCt                                                                                <  1:255107/101‑1 (MQ=255)
              gCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcg                                                                             >  2:4332974/1‑101 (MQ=255)
              gCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcg                                                                             >  2:1229745/1‑101 (MQ=255)
              gCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcg                                                                             >  1:3517284/1‑101 (MQ=255)
                ccAGCTGCGGGCCGGAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGcc                                                                           <  2:4245905/101‑1 (MQ=255)
                ccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGcc                                                                           >  2:2138160/1‑101 (MQ=255)
                ccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGcc                                                                           <  1:4319717/101‑1 (MQ=255)
                 cAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCg                                                                          >  2:2776128/1‑101 (MQ=255)
                 cAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCg                                                                          <  1:79945/101‑1 (MQ=255)
                 cAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCg                                                                          >  2:232980/1‑101 (MQ=255)
                   gCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCggg                                                                        >  1:3283393/1‑101 (MQ=255)
                    cTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGc                                                                       >  1:2381280/1‑101 (MQ=255)
                    cTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGc                                                                       <  2:4091120/101‑1 (MQ=255)
                     tGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGcc                                                                      <  1:1316711/101‑1 (MQ=255)
                        gggCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGAc                                                                   >  2:2712628/1‑101 (MQ=255)
                        gggCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGAc                                                                   >  2:3362935/1‑101 (MQ=255)
                          gCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCt                                                                 <  2:1281805/101‑1 (MQ=255)
                          gCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCt                                                                 >  2:1844454/1‑101 (MQ=255)
                            cGAAACGGTCGTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGACGACCTgg                                                               >  1:3848459/1‑101 (MQ=255)
                            cGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTgg                                                               >  1:2401084/1‑101 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                              <  1:3420019/101‑1 (MQ=255)
                              aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                             >  2:2519219/1‑101 (MQ=255)
                              aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                             >  2:2784668/1‑101 (MQ=255)
                               aaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgcg                                                            >  2:1567969/1‑101 (MQ=255)
                                  ggTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCgg                                                         >  2:3268224/1‑101 (MQ=255)
                                     ccTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAg                                                      <  2:3759015/101‑1 (MQ=255)
                                     ccTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAg                                                      <  1:2138299/101‑1 (MQ=255)
                                      cTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAgg                                                     <  1:1693622/101‑1 (MQ=255)
                                       ttGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGa                                                    >  2:2053462/1‑101 (MQ=255)
                                         gtgatgtgggAACTGGGGGATCGACAGGAAAACCTCCTGCGAGAGAGCGCCCCCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAtt                                                  <  1:1401502/93‑1 (MQ=255)
                                          aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                 <  1:2712818/101‑1 (MQ=255)
                                          aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGC..CGCGCGCGCGCAGCGGCGCCGCGGCGCCCCCGGCGCGGAAGGGGTcgc                                               >  2:3051376/1‑101 (MQ=255)
                                          aTCTGCTGGAACTGGTCGACGGACAGGAAGACCTGCGGCAAGAGATCGCGCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                 <  2:1503981/101‑1 (MQ=255)
                                           tCTGCTGGAACTGGTCGATCGACATGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcg                                                <  2:2654584/101‑1 (MQ=255)
                                           tCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcg                                                <  1:3324474/101‑1 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                               <  1:2283267/101‑1 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                               >  2:782955/1‑101 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                               >  1:3565890/1‑101 (MQ=255)
                                                 ggAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTg                                          <  1:4328251/101‑1 (MQ=255)
                                                 ggAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTg                                          >  1:1852893/1‑101 (MQ=255)
                                                 ggAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTg                                          <  2:2469659/101‑1 (MQ=255)
                                                  gAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                         >  1:4312404/1‑101 (MQ=255)
                                                   aaCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGGGCGCGCGCAGCTGCGCCGGGCAGACCTGGGCGCGGAAGCATTCGCGATTggg                                        >  1:1892398/1‑101 (MQ=255)
                                                     ctgtgcgatcgaCGGGAAGACCGGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGAGCGGAAGGATTCGCGATTGGGCa                                      <  2:1440867/96‑1 (MQ=255)
                                                      tGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAg                                     >  1:306294/1‑101 (MQ=255)
                                                       ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGc                                    >  1:276130/1‑101 (MQ=255)
                                                        gTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                   <  2:4267036/101‑1 (MQ=255)
                                                         tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                  <  1:3191595/101‑1 (MQ=255)
                                                           gatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                >  2:877066/1‑101 (MQ=255)
                                                            atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                               <  2:1315868/101‑1 (MQ=255)
                                                            atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                               >  1:2104898/1‑101 (MQ=255)
                                                             tcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTc                              <  1:2148269/101‑1 (MQ=255)
                                                             tcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCACGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTc                              <  1:3042722/101‑1 (MQ=255)
                                                              cgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCACGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCa                             >  2:2268616/1‑101 (MQ=255)
                                                               gaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAg                            <  2:431551/101‑1 (MQ=255)
                                                               gaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAg                            >  1:2056431/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                          >  2:835588/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                          >  2:3487620/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                          >  2:3921461/1‑101 (MQ=255)
                                                                  aGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcgc                         >  1:544219/1‑101 (MQ=255)
                                                                       gACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatcat                    <  2:2500017/101‑1 (MQ=255)
                                                                       gACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatcat                    >  2:4072011/1‑101 (MQ=255)
                                                                       gACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatcat                    <  1:1995054/101‑1 (MQ=255)
                                                                       gACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatcat                    <  1:3979836/101‑1 (MQ=255)
                                                                        aCCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATg                   <  2:3606646/101‑1 (MQ=255)
                                                                        aCCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATg                   <  1:3501050/101‑1 (MQ=255)
                                                                          ctgctgCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTGAGCGCATCATGCg                 >  1:2439078/1‑101 (MQ=255)
                                                                          ctgctgCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAACGATACGCGATTGGGCAGCCCGGTCAGCGCATCATGCg                 >  1:3805155/1‑101 (MQ=255)
                                                                           tgctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGt                >  1:1123665/1‑101 (MQ=255)
                                                                            gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc               >  1:2356905/1‑101 (MQ=255)
                                                                            gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc               >  2:1597683/1‑101 (MQ=255)
                                                                            gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc               <  1:2784858/101‑1 (MQ=255)
                                                                             ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg              >  1:1741023/1‑101 (MQ=255)
                                                                             ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg              <  1:63753/101‑1 (MQ=255)
                                                                              tgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgc             <  2:3517036/101‑1 (MQ=255)
                                                                              tgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgc             >  1:1065461/1‑101 (MQ=255)
                                                                               gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgcg            >  1:4159620/1‑101 (MQ=255)
                                                                               gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgcg            <  1:3390249/101‑1 (MQ=255)
                                                                                cAAGAGATCGCCCGCCGCGCGCGCGCGCAGGTGCGCCGGGCCGACCTGGGCGCGGAAGGATTGGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCgg           >  2:4242445/1‑101 (MQ=255)
                                                                                 aaGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGc          >  2:2599453/1‑101 (MQ=255)
                                                                                  agagATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCa         <  1:1290174/101‑1 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg       <  1:1946595/101‑1 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg       <  2:3795985/101‑1 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg       >  1:3457527/1‑101 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg       >  1:4374357/1‑101 (MQ=255)
                                                                                     gagcGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtgg      <  2:1053951/98‑1 (MQ=255)
                                                                                     gaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtgg      >  2:3953747/1‑101 (MQ=255)
                                                                                      aTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggc     <  2:3102915/101‑1 (MQ=255)
                                                                                         gCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg  >  2:2237372/1‑101 (MQ=255)
                                                                                                 |                                                                                            
CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGG  >  NC_014034/2731705‑2731894

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG