Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,740,181 G→T 12.8% G453C (GGC→TGC)  RCAP_rcc02544 → ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,740,1810G→T12.8% 4.9 ‑3.1 ‑0.239G453C (GGC→TGC) RCAP_rcc02544ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (15/19):  total (20/19)
Fisher's exact test strand distribution p-value = 4.71e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.17e-01

Only 100 of 128 total aligned reads displayed.

GCTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGATCG  >  NC_014034/2740081‑2740279
                                                                                                     |                                                                                                     
gcTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTg                                                                                                       <  2:294573/101‑1 (MQ=255)
gcTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTg                                                                                                       <  2:2297576/101‑1 (MQ=255)
gcTCGATGCCCGTCTTGGCCGGGTGCTCCCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTg                                                                                                       <  1:3925988/101‑1 (MQ=255)
 cTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGCGGGCCTTGGGGGCGGGGGGGACCGAATGGGACCGGATCCCCGACCGGC.TTgg                                                                                                      >  2:3112426/1‑101 (MQ=255)
   cGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.cc                                                                                                   <  2:1258159/101‑1 (MQ=255)
    gATTCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGAGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.Cc.g                                                                                                 <  1:1334184/101‑1 (MQ=255)
    gATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTgggcc                                                                                                   >  1:472368/1‑98 (MQ=255)
    gATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACCGGGACCGGGGCACCGACCGGC.TTgggac                                                                                                   >  1:906337/1‑98 (MQ=255)
     aTGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.cccc                                                                                                 >  1:2643746/1‑99 (MQ=255)
       gCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTT.Cc.gccc                                                                                              >  1:3265054/1‑99 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgcca                                                                                            <  2:862284/101‑1 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgcca                                                                                            <  2:2688321/101‑1 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgcca                                                                                            <  2:1595907/101‑1 (MQ=255)
          cGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgcca.g                                                                                          <  2:3077000/101‑1 (MQ=255)
           gTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.GC.CCgccccc                                                                                          >  2:2185460/1‑98 (MQ=255)
            tCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgccc.gcc                                                                                        >  1:3884143/1‑101 (MQ=255)
            tCTTGGCCGGGTGCTCTCGGGCGAGAGCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgcca.gcc                                                                                        <  1:3148061/101‑1 (MQ=255)
             cTTGGCGGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgcca.gcca                                                                                       <  2:4076005/101‑1 (MQ=255)
             cTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCgcca.gcca                                                                                       <  1:2962673/101‑1 (MQ=255)
              ttGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTTTGG.TC.GCgccgcgcg                                                                                        >  1:996844/1‑96 (MQ=255)
              ttGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTT.AC.GCgcctcacac                                                                                       >  2:2208000/1‑95 (MQ=255)
              ttGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGcca.gccag                                                                                      <  2:2311181/101‑1 (MQ=255)
               tGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.GC.GCGCcc.gccagc                                                                                     >  2:4207145/1‑101 (MQ=255)
                 gCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.CC.CCcccc.gcccccgc                                                                                   >  1:614308/1‑96 (MQ=255)
                 gCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAgcgc                                                                                   <  2:3510367/101‑1 (MQ=255)
                   cGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGcgccg                                                                                 <  2:3379548/101‑1 (MQ=255)
                    gggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.GC.GCGCCC.GCCCGcgcccc                                                                                >  2:337732/1‑99 (MQ=255)
                     ggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTT.TC.CCGGCA.CCCCGCgccgcc                                                                               >  2:4432947/1‑101 (MQ=255)
                      gTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGAGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCa                                                                              <  1:3902481/101‑1 (MQ=255)
                       tGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCAc                                                                             >  2:2737779/1‑101 (MQ=255)
                        gCTTTCGGGCGAGATCCCCCCCGATGCTTCTCTGGCGGCGGCGGCTCCCGACTGGGACCGAATCCCTGCCCGGT.TTGG.CC.GCGCCA.GCCAGTGCCGTCACt                                                                            <  2:156413/101‑1 (MQ=255)
                        gCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTT.CC.CCGCCC.CCCCGCgccgccccc                                                                            >  1:3392881/1‑98 (MQ=255)
                           ctggggCGAGATCACCCCGGAGGCGGCTCGGGCGGCGGCGGCGACCGACGGGGACCGGATCACCGACCGGC.TTGC.CC.GCGCCA.GCCAGCGCCGCCACTAcc                                                                         <  1:3648694/98‑1 (MQ=255)
                           ctcGGGCGAGATCCCCCCCGGGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGCCCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTAcc                                                                         <  2:4129038/101‑1 (MQ=255)
                           ctcGGGCGAGATCCCCCCCGAGGCGGCTCTGGCGGCGGCGGCGCCCGACTGGGCCCGGATCCCCGACCGGC.TTGC.CC.GCGCCA.GCCAGCGCCGCCACTAcc                                                                         <  1:4145498/101‑1 (MQ=255)
                           ctcGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTT.GC.GCGGCC.GCCAGCGCCGCCCCCAcc                                                                         >  1:2655242/1‑101 (MQ=255)
                            tcGGGCGAGCTCCCCCCCGGGGCGTTTCTGGCGGCGGGGGCAACCGACTGGAACCGGATCACCGCCCGTC.TTGG.CC.CCGCCA.GCCAGCGCCGCCACTACcg                                                                        <  1:548005/101‑1 (MQ=255)
                            tcGGGCGAGACCCCCCCCGGGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCCCCGCCCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACcg                                                                        <  2:2557706/101‑1 (MQ=255)
                             cGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.CC.GCGCCC.GCCCCCgccgcccccccccc                                                                       >  1:1743641/1‑93 (MQ=255)
                             cGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGAACAAGCACCAGC.TTGT.CT.CCGCCC.GCCAGGGTGGCCGCTAcccg                                                                       >  2:1050555/1‑99 (MQ=255)
                              gggCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.CC.GCGCCC.CCCACCggcgccgcccccccg                                                                      >  2:2683316/1‑92 (MQ=255)
                                gCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.TC.GCGCCC.CCCCGCGCCCCCCCCACcccgcg                                                                    >  1:2369608/1‑99 (MQ=255)
                                gCGAGACCCCCCCGGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCCCCGACCGGC.TTGC.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAg                                                                    <  1:328685/101‑1 (MQ=255)
                                     ctccccccgGAGGCGGCTCTGGCGGCGGCGGCGACCGACGGGGACCGGATCCCCGACCGGC.TGGC.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGAt                                                               <  2:825392/97‑1 (MQ=255)
                                     acccccccgGGGGGGGCTCGGGGGGGGGGGGCGCCCGACGGGGACGGGATCCCCGCCCGGT.TTGG.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGAt                                                               <  1:337767/97‑1 (MQ=255)
                                     accccccccGAGGCGGCTCTGGGGGCGGCGGCGACCGACTGGGACCGGATCCCCGCCCGGC.TGGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGAt                                                               <  2:1743522/97‑1 (MQ=255)
                                     aTCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.CCGCCA.GCCAGCGCCGCCCCCACCCCGAGGCggt                                                               >  1:1211875/1‑99 (MQ=255)
                                       cACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTG.CC.CCGCCC.GCCCGCGCCCCCCCCACCCCGCGGCggggg                                                             >  1:2736947/1‑97 (MQ=255)
                                         ccccgGGGGGGGCTCGGGGGGCGCCGGCGCCCAGCTGGGCCGGGACCACCGACCGGT.TGGG.CG.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATgggg                                                           <  1:444682/101‑1 (MQ=255)
                                           ccgggggcggcGCGGGGGGCGGGGGCGCCCGGGTGGGCCGGGTCCCCCGCCGGGT.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCt                                                         <  1:3111469/96‑1 (MQ=255)
                                           cccGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGGCC.GCCACCGCCGCCACTACCGCGAGGCGCTGGGGCt                                                         >  1:283337/1‑101 (MQ=255)
                                            cgggggcggcTCGGGGGGGGGCGGCGCCGGACGGGGCCCGGACCCCCGCCGGGC.TGGC.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTg                                                        <  2:3136940/97‑1 (MQ=255)
                                             cGAGGGGGCTCGGGCGGCGGCGGCGCCCGACGGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTgg                                                       <  1:2151511/101‑1 (MQ=255)
                                              gggggggcTCGGGGGGCGGGGGCGCCCGACGGGGACCGGATCCCCGACCGGT.TTGG.CC.GCGCCA.GCCAGGGCCGCCACTACCGCGAGGCGATGGGGCTGGa                                                      <  1:3962898/96‑1 (MQ=255)
                                               gggcggcTCGGGCGGCGGCGGCGCCCGGCTGGGACCGGATCCCCGCCCGGC.TGGG.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAg                                                     <  2:1463214/100‑1 (MQ=255)
                                                gggggcTCGGGCGGCGGCGGCGCCCGACGGGGACCGGACCCCCGCCCGGC.TTGC.CC.CCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAgg                                                    <  2:1911624/98‑1 (MQ=255)
                                                ggcggcTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCC.GCCAGCGCCGCCACTACCGCGCGGGGGTGGGGCTGGAgg                                                    >  2:1602447/1‑101 (MQ=255)
                                                ggcggcTCGGGGGGCGGGGGCGACCGCCTGGGCCCGGACCCCCGCCGGGC.TTGC.CC.CCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAgg                                                    <  2:206468/101‑1 (MQ=255)
                                                 gcggcTCTGGCGGCGGGGGCGCCCGCCGGGCCCCGGATCACCGCCCGGT.TTGG.CC.GCCCCA.GCCGGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAggc                                                   <  2:2906472/101‑1 (MQ=255)
                                                  cggcTCGGGCGGCGGCGGCGACCGACTGGGCCCGGTTCACCACCCGGT.TGGG.CC.CCGCCA.GCCAGCCCCCCCACTACCGCGAGGCGATGGGGCTGGAggcg                                                  <  2:2920193/101‑1 (MQ=255)
                                                     ctctGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTTT.CC.GCGGCC.ACCCGCGCCGCCCCCCCCGCGAGGCGACGGGGCCGGAGgcggcg                                               >  1:1488637/1‑101 (MQ=255)
                                                         gggggggggggcgACCAACTGGGCCCGTATCCCCCTCGGTT.TTCG.CC.CCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCAt                                           <  2:163796/92‑1 (MQ=255)
                                                         ggcggcggcggcGACCGACTGGGACCGGATCACCGACCGGC.TTTG.CC.CCGCCC.GCCCCCGCCCCCCCCCCCACCGGGCGGTGGGGGTGGGggggggggcct                                           >  1:2152738/1‑95 (MQ=255)
                                                          gcggcggcggcgCCCGCCGGGGCCCGGACCCCCGACCGGC.TGGG.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATg                                          <  1:9524/101‑1 (MQ=255)
                                                           ggggggcggcgCCCGAGGGGGCCCGGATCCCCGCCCGGC.TTGG.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGa                                         <  2:50534/97‑1 (MQ=255)
                                                           gggcggcggcgACCGACGGGGCCGGGATCCCCGCCGGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGa                                         <  1:3112650/100‑1 (MQ=255)
                                                            ggcggcggcgACCGACTGGGACCGGATCACCGACCGGC.TTTG.GC.GCGGCAGGCCAGC.CCGCCCCCCCCCCCCGGGGATGTGGCTGGAGGGGGGGCCACGAg                                        >  2:1364604/1‑101 (MQ=255)
                                                               ggcggcaCCCGACGGGGCCCGGACCCCCGCCGGGC.TGGC.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCt                                     <  2:3128578/101‑1 (MQ=255)
                                                                gcggggCCCGCCGGGGACGGGACCCCCGCCCGGT.TGGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtg                                    <  1:3683241/101‑1 (MQ=255)
                                                                 gggcgCCCGACGGGGCCGGGACCCCCGCCCGGC.TGGC.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgt                                   <  1:317097/100‑1 (MQ=255)
                                                                 cggcgACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGGGCCATGAGCCtgg                                   >  2:148217/1‑100 (MQ=255)
                                                                  ggcgCCCGACGGGGCCGGGCTCCCGGCCCGGT.TGGC.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgtg                                  <  2:3533797/101‑1 (MQ=255)
                                                                  ggcgCCCGACGGGGCCGGGACCCCCGGCCGGC.TGGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgtg                                  <  2:4352796/101‑1 (MQ=255)
                                                                  ggcgACCGCGGGGGCCCGGACCCCCGCCCGGC.TGGC.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgtg                                  <  1:1079699/101‑1 (MQ=255)
                                                                  ggcgACCGACTGGGACCGGATCACCGACCGGC.TTTG.CC.GCGCCA.GCCATCGCCGCCCCTACCGCCGGGCGGTGGGGCGGGGGGCGGGGCCCTGAGcacgtg                                  >  2:1472449/1‑96 (MQ=255)
                                                                    cgcccgccggggACGGGACCCCCGCCCGGC.TGGG.CC.CCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGgc                                <  1:4208800/92‑1 (MQ=255)
                                                                      cccgagggggCCCGGTTCCCCGCCCGGC.TGGC.CC.CCGCCA.CCCAGCCCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGgcgc                              <  1:2737969/94‑1 (MQ=255)
                                                                       cgcccggggACCAGAGCCCCGGCCGCC.TGGC.CC.GCCCCA.GCCGGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGcgcg                             <  1:1050643/95‑1 (MQ=255)
                                                                        cggcggggGCCGGAACCCCCGCCGGT.TTGGCCC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGcgcg                             <  1:3799038/96‑1 (MQ=255)
                                                                        cgacTGGGACCGGATCACCGACCGGC.TTTG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGGTGGGGGCGGGGCCCTGAGCCCGTTGGGCgg                            >  2:1237290/1‑101 (MQ=255)
                                                                         gacTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCCCTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCCTGAGCCTGTGGGGCGGc                           >  1:4390276/1‑101 (MQ=255)
                                                                          gcggggCCCGGACCCCCGCCGGGC.TGGC.CC.GCGCCA.CCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCa                          <  1:2015298/98‑1 (MQ=255)
                                                                            tGGGACCGGATCACCGACCGGC.TTTG.CC.GCGCCC.GGCCGCGCCGCCCCCACCGCGGGGCGATGGGGGTGGGGGGGGGGCCCTGCGGCTGTggggggggcat                        >  1:4148973/1‑92 (MQ=255)
                                                                            tGGGACCGGATCACCGACCGGC.TTTG.CC.GCGCCA.GCCCGCGCCGCCCCTACCGCGAGGCGATGGGGCTGGGGGGGGGGCCCTGAGCCTGTGGCGCGGCAtt                        >  1:2585738/1‑101 (MQ=255)
                                                                             gggACCGGATCACCGACCGGC.TTTG.CC.GCGCCC.GCCAGCGCCCCCCCCACCGCCGGGCGGTGGGGCTGGGGGGGGGGCCATGAGCCTGCGGGGCGGccata                       >  1:1155827/1‑97 (MQ=255)
                                                                             gggACCGGATCACCGACCGGC.TTTG.CC.GCGCCC.CCCCGCGCCGCCCCCACCCCGGGGCGATGGGGGTGGGGGGGGGGCCCTGAGGCCGTgggcccggggtt                       >  1:4347427/1‑91 (MQ=255)
                                                                             gggACCGGATCACCGACCGGC.TTGG.GC.GCGCCC.GCCAACACCCCCCCTACCGCGAGGCGAAGGGGATGGAGGCCGCGCCACGAGGCCCTGGCGCgggcata                       >  1:3482567/1‑96 (MQ=255)
                                                                             gggACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCCCTACCGCGAGGCGATGGGGCTGGAGGGGGCGCCATGAGCCTGTGGCGCGGCATTc                       >  1:1397060/1‑101 (MQ=255)
                                                                             gggACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTc                       >  1:2906866/1‑101 (MQ=255)
                                                                             ggccccGGACCCCCGCCCGGC.TGCG.CC.CCCCCA.GCCGGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTc                       <  2:3981087/97‑1 (MQ=255)
                                                                                aCCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAgg                    >  1:1859929/1‑101 (MQ=255)
                                                                                  cGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCCCCCCCATCCCGCGGCGATGGGGCTGGAGGCGGCCCCACGATGCCGTGGCGCGGGACTCCgggg                  >  2:771661/1‑101 (MQ=255)
                                                                                   ggATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGGGGCGGCGCCATGAGCCTTTGGCGCGGCATTCAggggg                 >  2:1384883/1‑100 (MQ=255)
                                                                                   ggATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAggggg                 >  2:4352860/1‑100 (MQ=255)
                                                                                     aTCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCCCGAGGCGATGGGGCTGGAGGGGGCGCCATGAGCCCGTGGGGCGGCATTCAGGggcgg               >  1:2071631/1‑101 (MQ=255)
                                                                                       cACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCt             >  2:392107/1‑101 (MQ=255)
                                                                                        ccgccccgGC.TGGC.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTg            <  1:3598848/96‑1 (MQ=255)
                                                                                          cgcccgGC.TGGC.CC.GCCCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGtt          <  1:2502221/98‑1 (MQ=255)
                                                                                             gcccggttGG.CCGGCCCCG.GCCCGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTg         <  1:3765370/95‑1 (MQ=255)
                                                                                             ccgGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCt       >  2:3485868/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GCTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGC.TTGG.CC.GCGCCA.GCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGATCG  >  NC_014034/2740081‑2740279

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG