Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,854	C→G	100%	intergenic (‑186/+107)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,854	0	C→G	100.0%	69.3	24	intergenic (‑186/+107)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (7/17):  ref base (0/0):  total (7/17)

TAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747761‑2747950                                                                                               |                                                                                                    tAGAAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                               <  2:1032761/101‑1 (MQ=25) aaGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                               <  2:3037013/100‑1 (MQ=25)  aGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                              <  1:2444692/101‑1 (MQ=25)   ggaacggccccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                             <  1:545456/91‑1 (MQ=25)   ggAAACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                             <  1:955445/101‑1 (MQ=37)   ggAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                             >  2:4194194/1‑101 (MQ=255)    gAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgcg                                                                                            <  1:1223420/101‑1 (MQ=25)     aaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgcgc                                                                                           <  2:1698072/98‑1 (MQ=25)       ccgcaccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                         <  1:3181821/95‑1 (MQ=25)         cccccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccgc                                                                                       <  1:2102195/97‑1 (MQ=21)          cgccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccgcc                                                                                      <  2:1802792/98‑1 (MQ=21)            ccccGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                   <  1:747995/100‑1 (MQ=255)            ccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                   <  2:188383/100‑1 (MQ=25)              ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                 <  1:702708/101‑3 (MQ=25)              ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                 <  2:2395303/101‑3 (MQ=25)               cgctTTCCCCCGCCCGCGGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                <  2:4142621/101‑4 (MQ=21)               cgctTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                <  2:4133233/101‑4 (MQ=21)                   tccccccGCCCGCAGCGCCCGGTTGCGGCAGGACCGCTTCAGCCCGCACCACAGGGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                            <  1:3237609/98‑8 (MQ=21)                    ccccccGCCCGCAGCGCCCGGTTGCGGCAGGACCGCTTCACCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggcg                                                                           <  1:4116955/99‑9 (MQ=18)                                                                                cgcCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGc              >  2:4142135/1‑101 (MQ=18)                                                                                 gcccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg             >  1:490430/1‑101 (MQ=18)                                                                                   ccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt           >  1:1645388/1‑101 (MQ=18)                                                                                     ggcccgGCGGGCGAGAAGCGCCCGCC.GGGACCGGC.GCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCGCAGGCCCTCTGTGACGCATGGCCAAGGCGATgg         >  1:3661614/1‑101 (MQ=18)                                                                                       cccggcgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGcc       >  2:1361967/5‑101 (MQ=18)                                                                                        ccggcgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCACGGCGATGGCCt      >  2:3374190/4‑101 (MQ=18)                                                                                            cgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGCTGGCCTTGGc  >  2:1885540/1‑101 (MQ=21)                                                                                               |                                                                                                    TAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747761‑2747950 Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG