Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,770,015 C→G 100% H311Q (CAC→CAG RCAP_rcc02581 → sensor histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,770,0150C→G100.0% 741.8 196H311Q (CAC→CAGRCAP_rcc02581sensor histidine kinase
Reads supporting (aligned to +/- strand):  new base (112/84):  ref base (0/0):  total (112/84)

Only 100 of 202 total aligned reads displayed.

GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112
                                                                                                  |                                                                                                 
gagaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGt                                                                                                 >  2:4017181/1‑101 (MQ=255)
gagaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGt                                                                                                 <  1:2009791/101‑1 (MQ=255)
 agaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTg                                                                                                >  2:1154716/1‑101 (MQ=255)
 agaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTg                                                                                                >  2:3863872/1‑101 (MQ=255)
 agaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTg                                                                                                <  2:3136882/101‑1 (MQ=255)
  gaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGAGCAGGTgg                                                                                               >  2:1952484/1‑101 (MQ=255)
   aTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGt                                                                                              >  2:149373/1‑101 (MQ=255)
    tGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTc                                                                                             <  1:3532254/101‑1 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            >  2:544826/1‑101 (MQ=255)
         aaGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        >  2:2189205/1‑101 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     <  1:1227797/101‑1 (MQ=255)
             tgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGcc                                                                                    <  1:3559011/101‑1 (MQ=255)
              gatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCg                                                                                   >  2:3041509/1‑101 (MQ=255)
              gatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCg                                                                                   <  1:3402514/101‑1 (MQ=255)
              gatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCg                                                                                   >  1:11110/1‑101 (MQ=255)
               atCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGt                                                                                  >  1:3929477/1‑101 (MQ=255)
               atCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGt                                                                                  <  1:959961/101‑1 (MQ=255)
               atCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGt                                                                                  <  1:1546609/101‑1 (MQ=255)
               atCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGt                                                                                  >  1:3187837/1‑101 (MQ=255)
                tCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTg                                                                                 <  2:368142/101‑1 (MQ=255)
                tCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTg                                                                                 <  1:3325580/101‑1 (MQ=255)
                tCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTg                                                                                 <  2:3229277/101‑1 (MQ=255)
                  gACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcg                                                                               <  2:2772954/101‑1 (MQ=255)
                  gACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcg                                                                               >  1:3029100/1‑101 (MQ=255)
                  gACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcg                                                                               >  2:4190918/1‑101 (MQ=255)
                  gACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcg                                                                               >  1:3260445/1‑101 (MQ=255)
                   aCGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcgc                                                                              >  1:2202170/1‑101 (MQ=255)
                     gCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcgcgc                                                                            >  1:570758/1‑101 (MQ=255)
                      ccTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGcc                                                                           <  2:2960557/101‑1 (MQ=255)
                        ttCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCgg                                                                         >  1:294399/1‑101 (MQ=255)
                        ttCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGGGGTCGAAGATGCCGTGCGCGCCgg                                                                         >  1:2819497/1‑101 (MQ=255)
                           cTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGTAGATGCCGTGCGCGCCGGGCa                                                                      <  1:2545658/101‑1 (MQ=255)
                             gAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGc                                                                    >  1:165762/1‑101 (MQ=255)
                               aTGTCGCGCGGGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCg                                                                  <  1:4441639/101‑1 (MQ=255)
                               aTGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCg                                                                  <  1:4104713/101‑1 (MQ=255)
                               aTGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCg                                                                  >  2:211068/1‑101 (MQ=255)
                               aTGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGGGGTCGAAGATGCCGTGCGCGCCGGGCAGCCg                                                                  >  1:4009575/1‑101 (MQ=255)
                                   cgcgcgtgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGc                                                              >  1:2094593/1‑101 (MQ=255)
                                    gcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCa                                                             <  2:4304610/101‑1 (MQ=255)
                                    gcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCa                                                             <  2:1694188/101‑1 (MQ=255)
                                         cgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtgg                                                        <  2:2338656/101‑1 (MQ=255)
                                         cgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtgg                                                        >  1:1519123/1‑101 (MQ=255)
                                          gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggt                                                       >  2:83936/1‑101 (MQ=255)
                                          gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggt                                                       <  2:570690/101‑1 (MQ=255)
                                          gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggt                                                       >  2:2525569/1‑101 (MQ=255)
                                          gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggt                                                       >  2:4132432/1‑101 (MQ=255)
                                          gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggg                                                       >  1:824473/1‑100 (MQ=255)
                                             gagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGc                                                    <  1:3864125/101‑1 (MQ=255)
                                             gagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGc                                                    <  2:3109975/101‑1 (MQ=255)
                                             gagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGc                                                    <  2:1407824/101‑1 (MQ=255)
                                              agGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGcc                                                   <  1:2834397/101‑1 (MQ=255)
                                              agGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGcc                                                   >  1:271802/1‑101 (MQ=255)
                                                  gATGGCGGGCGAGCCCGAACGGGTGGAGATTTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcc                                               >  2:3414380/1‑101 (MQ=255)
                                                   aTGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGccc                                              <  2:2932609/101‑1 (MQ=255)
                                                   aTGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGccc                                              >  2:1127975/1‑101 (MQ=255)
                                                    tGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcccc                                             <  2:3727361/101‑1 (MQ=255)
                                                    tGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcccc                                             <  2:2706305/101‑1 (MQ=255)
                                                    tGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcccc                                             >  2:1870259/1‑101 (MQ=255)
                                                    tGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcccc                                             <  2:490359/101‑1 (MQ=255)
                                                      gcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGa                                           >  1:1779351/1‑101 (MQ=255)
                                                       cgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGAt                                          <  2:1184715/101‑1 (MQ=255)
                                                        gggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTTGCCTCCCCCGATg                                         <  2:1692863/101‑1 (MQ=255)
                                                         ggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgc                                        >  2:1233967/1‑101 (MQ=255)
                                                          gcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcg                                       >  2:1622091/1‑101 (MQ=255)
                                                           cgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcgc                                      <  1:1427316/101‑1 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     <  1:3802185/101‑1 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAAATGCCGTTCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     <  1:3684451/101‑1 (MQ=255)
                                                                ccGAACGGGTGGAGAGCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTg                                 <  2:2019106/101‑1 (MQ=255)
                                                                  gAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCa                               >  1:2588049/1‑101 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              >  2:2829571/1‑101 (MQ=255)
                                                                    aCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAgg                             >  1:836059/1‑101 (MQ=255)
                                                                        gTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGt                         >  2:1534095/1‑101 (MQ=255)
                                                                        gTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGt                         >  1:4328677/1‑101 (MQ=255)
                                                                         tGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtg                        >  1:3427650/1‑101 (MQ=255)
                                                                         tGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtg                        >  2:1091799/1‑101 (MQ=255)
                                                                           gagaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgct                      >  1:3916912/1‑101 (MQ=255)
                                                                           gagaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgct                      >  2:379165/1‑101 (MQ=255)
                                                                           gagaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgct                      <  1:979716/101‑1 (MQ=255)
                                                                            agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGGGCAGCTGGTGGCCTGCCCCGATCCGCCGGGGCAGGCGGtgctc                     >  1:267278/1‑100 (MQ=255)
                                                                              aTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTgcg                   >  1:248640/1‑101 (MQ=255)
                                                                               tCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGTCCGTGCAGGGGGGGGTgcgc                  >  2:1928454/1‑101 (MQ=255)
                                                                               tCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTgcgc                  <  2:1021352/101‑1 (MQ=255)
                                                                                 tGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGccc                >  2:3525379/1‑101 (MQ=255)
                                                                                   cgcCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCg              <  1:818673/101‑1 (MQ=255)
                                                                                   cgcCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGGGCTGCGCCCCg              >  1:1025571/1‑101 (MQ=255)
                                                                                      cTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtg           >  1:4030909/1‑101 (MQ=255)
                                                                                      cTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtg           >  1:3117194/1‑101 (MQ=255)
                                                                                       tttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgc          <  1:115989/101‑1 (MQ=255)
                                                                                       tttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgc          >  2:766322/1‑101 (MQ=255)
                                                                                        ttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtggg         >  2:1710409/1‑99 (MQ=255)
                                                                                        ttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcg         >  2:1461099/1‑101 (MQ=255)
                                                                                         tGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGAGCAGGCGGTGCTGCGCCCCGAtgcgc        >  2:1431245/1‑101 (MQ=255)
                                                                                            cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgc     <  1:3608014/101‑1 (MQ=255)
                                                                                            cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgc     <  1:4191179/101‑1 (MQ=255)
                                                                                             gcgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgcg    <  1:2129168/101‑1 (MQ=255)
                                                                                             gcgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgcg    >  2:1366744/1‑101 (MQ=255)
                                                                                             gcgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgatgcg    >  2:2077652/1‑101 (MQ=255)
                                                                                              cgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGGGCTGCGCCCCGAtgcgctgcgc   >  1:455529/1‑101 (MQ=255)
                                                                                               gcAGGTGGTCGACGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCGCCGGTGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCCcc  <  2:715849/101‑1 (MQ=255)
                                                                                               gcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGcc  <  2:3561723/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG