Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,808,441 C→T 100% P352L (CCC→CTC)  pyrD2 → dihydroorotate oxidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,808,4410C→T100.0% 848.5 224P352L (CCC→CTC) pyrD2dihydroorotate oxidase
Reads supporting (aligned to +/- strand):  new base (98/125):  ref base (0/0):  total (98/126)

Only 100 of 232 total aligned reads displayed.

TCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCCCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCG  >  NC_014034/2808343‑2808538
                                                                                                  |                                                                                                 
 cTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTg                                                                                                >  2:247753/1‑101 (MQ=255)
 cTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTg                                                                                                <  1:1603503/101‑1 (MQ=255)
  tGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGa                                                                                               >  2:3382542/1‑101 (MQ=255)
   ggcggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAc                                                                                              >  1:229735/1‑101 (MQ=255)
   ggcggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAc                                                                                              >  2:3312031/1‑101 (MQ=255)
     cggcCGAGGTCGCGCGCGGCCTGGTTGCGCTTTTGGTGCGCGACGGCTTTGCTTCGGTCGCGGAACTCGCGGGCACCGGTCGCCGCCGCTGGCTCTGAccc                                                                                            >  2:4233814/1‑101 (MQ=255)
       gcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgc                                                                                          >  2:3015160/1‑101 (MQ=255)
         cGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgccc                                                                                        >  1:3665663/1‑101 (MQ=255)
          gAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcccg                                                                                       >  1:2249456/1‑101 (MQ=255)
            ggTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTa                                                                                     >  1:1133496/1‑101 (MQ=255)
              tCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGc                                                                                   >  2:3783114/1‑101 (MQ=255)
               cgcgcgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCt                                                                                  <  2:201224/101‑1 (MQ=255)
               cgcgcgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCt                                                                                  <  2:2778125/101‑1 (MQ=255)
                gcgcgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTg                                                                                 >  1:2372712/1‑101 (MQ=255)
                 cgcgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGc                                                                                <  2:435595/101‑1 (MQ=255)
                  gcgcgCCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCCCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGAAGCTTCg                                                                               >  1:3289206/1‑101 (MQ=255)
                   cgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCgg                                                                              <  1:5678/101‑1 (MQ=255)
                    gcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCggg                                                                             >  2:3232676/1‑101 (MQ=255)
                      ggCCTGGATGGGCTTTTGGCGCGGGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCggggg                                                                           <  1:1400129/101‑1 (MQ=255)
                      ggCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCggggg                                                                           >  2:107043/1‑101 (MQ=255)
                       gCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCgggggg                                                                          >  2:1774382/1‑100 (MQ=255)
                         cTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGgtt                                                                        >  2:2710759/1‑101 (MQ=255)
                          tGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTc                                                                       <  2:2397454/101‑1 (MQ=255)
                          tGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTc                                                                       >  2:1370082/1‑101 (MQ=255)
                           ggATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTca                                                                      >  1:627196/1‑101 (MQ=255)
                             aTGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCCCTGAGCCGCCCGTAGCTGCgggggggtgcaa                                                                    >  1:1046225/1‑94 (MQ=255)
                              tGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCacac                                                                   >  2:2121512/1‑101 (MQ=255)
                                cgcTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCAcaccc                                                                 >  1:3133793/1‑101 (MQ=255)
                                 gcTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccc                                                                <  1:3514962/101‑1 (MQ=255)
                                  cTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcaccccc                                                               <  1:1157208/101‑1 (MQ=255)
                                  cTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcaccccc                                                               <  1:3572105/101‑1 (MQ=255)
                                  cTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCAcaccccc                                                               >  1:4020833/1‑101 (MQ=255)
                                  cTTTGGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcaccccc                                                               <  1:738288/101‑1 (MQ=255)
                                   ttttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCTGGGGTTTCAcacccccg                                                              >  2:456879/1‑101 (MQ=255)
                                   ttttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCAcacccccg                                                              >  2:3718932/1‑101 (MQ=255)
                                     ttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgca                                                            <  2:1385050/101‑1 (MQ=255)
                                        gcgcgcGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcaccc                                                         <  1:2800956/101‑1 (MQ=255)
                                          gcgcgACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcaccccc                                                       <  1:659035/101‑1 (MQ=255)
                                          gcgcgACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcaccccc                                                       <  2:1960009/101‑1 (MQ=255)
                                           gtccaCGGTTTTGCTTCGGTGGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcacccccg                                                      <  2:1729954/97‑1 (MQ=37)
                                           cgcgCCGGCTTTGTTTCGGTCGCGGAAGCGGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcacccccg                                                      <  1:3359851/101‑1 (MQ=37)
                                             cgACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCAcacccccccacccccgcg                                                    >  2:2870889/1‑99 (MQ=255)
                                              gagggCTTTGCTTCGGGCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTgg                                                   <  2:1920083/98‑1 (MQ=255)
                                                cGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCg                                                 <  2:3025357/101‑1 (MQ=255)
                                                  gCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTa                                               <  1:2321889/101‑1 (MQ=255)
                                                    tttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCCCACCCCCGTGGCGGAtt                                             >  2:4305070/1‑101 (MQ=255)
                                                     ttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  2:386958/101‑1 (MQ=255)
                                                     ttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  2:658621/101‑1 (MQ=255)
                                                     ttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  1:2755253/101‑1 (MQ=255)
                                                     ttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  1:256042/101‑1 (MQ=255)
                                                     tggCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  1:376558/99‑1 (MQ=255)
                                                      tGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCGCCCCCGTGGCGTATTTg                                           <  1:2873958/101‑1 (MQ=255)
                                                      tGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTg                                           <  1:2726021/101‑1 (MQ=255)
                                                       gCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCCCACCCCCCCACCCCCCGGGCGTATTTgc                                          >  1:613221/1‑101 (MQ=37)
                                                        cTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTgcg                                         <  1:1425358/101‑1 (MQ=255)
                                                          tCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGcc                                       <  2:2528570/101‑1 (MQ=255)
                                                          tCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCACCCCCGTGGCGTATTTGCGcc                                       >  1:3032650/1‑101 (MQ=255)
                                                           gggTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCa                                      <  2:813860/100‑1 (MQ=255)
                                                            ggccgcgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCaa                                     <  2:1290528/98‑1 (MQ=255)
                                                            ggacgcgGAAGCCGGGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGCAGCTGCGGGGGCTTCCGACCCCCGCACCCCCGCGGCGTACTTGCGcccg                                     >  2:3182738/4‑99 (MQ=21)
                                                            ggTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCCCCCCCGTGGCGTCTTTGCGCCaa                                     >  1:1709858/1‑101 (MQ=255)
                                                             gTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAg                                    <  1:3079336/101‑1 (MQ=255)
                                                               cggggAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaa                                  <  1:338353/98‑1 (MQ=255)
                                                               cgcgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaa                                  <  2:682314/101‑1 (MQ=255)
                                                                gcgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaa                                 >  1:2162130/1‑101 (MQ=255)
                                                                 gggAAGCCGTGGGCCCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaaa                                <  1:863787/100‑1 (MQ=255)
                                                                 cgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCACCCCCGGGGCGGATTTGCGCCCAGaaaa                                >  1:1549192/1‑101 (MQ=25)
                                                                  ggAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaaaa                               >  2:402782/1‑101 (MQ=255)
                                                                  ggAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaaaa                               >  2:1664760/1‑101 (MQ=255)
                                                                   gAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAg                              <  1:3914906/101‑1 (MQ=255)
                                                                   gAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAg                              >  1:3786825/1‑101 (MQ=255)
                                                                       ccGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGcaca                          <  1:384714/101‑1 (MQ=255)
                                                                        cGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAg                         >  1:4120394/1‑101 (MQ=255)
                                                                        cGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAg                         <  2:1795798/101‑1 (MQ=255)
                                                                         gTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGa                        >  1:2672340/1‑101 (MQ=255)
                                                                           gggCCCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACt                      <  1:1361303/101‑1 (MQ=255)
                                                                           gggCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACt                      <  2:3522413/101‑1 (MQ=255)
                                                                             gccccGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACttt                    <  2:1524850/98‑1 (MQ=255)
                                                                               cccGCTCGCAGCCGCTGGTTCTGACCCGCCCGTAGCTGCGGGTGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTg                  <  2:3317543/100‑1 (MQ=17)
                                                                               aCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTg                  <  2:406934/101‑1 (MQ=255)
                                                                               aCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTg                  >  1:3016967/1‑101 (MQ=255)
                                                                                ccGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGc                 <  2:3822421/101‑1 (MQ=255)
                                                                                ccGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGc                 <  1:3872364/101‑1 (MQ=255)
                                                                                 cGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGcc                <  2:59148/101‑1 (MQ=255)
                                                                                 cGGTCGCAGCCGCTGGCGCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGcc                <  2:1302152/101‑1 (MQ=255)
                                                                                  ggTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGccc               >  1:1919281/1‑101 (MQ=255)
                                                                                   gTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCg              >  1:1183361/1‑101 (MQ=255)
                                                                                   gTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCg              <  2:118566/101‑1 (MQ=255)
                                                                                    tCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCTGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGt             <  1:3822440/101‑1 (MQ=255)
                                                                                    tCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGt             <  2:3665410/101‑1 (MQ=255)
                                                                                      gCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTc           <  2:278339/101‑1 (MQ=255)
                                                                                      gCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTc           >  1:4164448/1‑101 (MQ=255)
                                                                                        aGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCAc         <  1:2700800/101‑1 (MQ=255)
                                                                                           tgCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCt      >  2:545235/2‑101 (MQ=255)
                                                                                           cGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCTCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCt      <  1:890039/101‑1 (MQ=255)
                                                                                           cGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCt      <  1:1638181/101‑1 (MQ=255)
                                                                                            gCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTg     <  1:4318887/101‑1 (MQ=255)
                                                                                             cTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTgg    >  2:2237242/1‑101 (MQ=255)
                                                                                              tGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGgc   >  1:4300517/1‑101 (MQ=255)
                                                                                               ggCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGgcg  <  1:1795765/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
TCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCCCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCG  >  NC_014034/2808343‑2808538

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG