Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,819,668 G→C 11.1% A73G (GCG→GGG)  dacB ← penicillin‑binding protein 4

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,819,6680G→C11.1% 3.4 ‑1.2 ‑0.045A73G (GCG→GGG) dacBpenicillin‑binding protein 4
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (11/29):  total (11/34)
Fisher's exact test strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.51e-01

GACCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATCAG  >  NC_014034/2819568‑2819768
                                                                                                      |                                                                                                    
gaCCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAggc.gggcg                                                                                                      >  1:1008555/1‑101 (MQ=255)
 gccGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGgcgc                                                                                                     <  1:266758/100‑1 (MQ=255)
 aCCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.CGgggc                                                                                                     >  2:1384696/1‑101 (MQ=255)
  ccGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGcc                                                                                                    >  2:4089235/1‑101 (MQ=255)
   cGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGccc                                                                                                   >  2:4436251/1‑100 (MQ=255)
   cGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCa                                                                                                   <  1:956033/101‑1 (MQ=255)
   cGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCa                                                                                                   >  2:3223002/1‑101 (MQ=255)
   cGGCCCCGTCGCCAGCACCCGGGTCGGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCa                                                                                                   <  2:3729525/101‑1 (MQ=255)
    ggCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCAt                                                                                                  <  2:3506959/101‑1 (MQ=255)
      ccccGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGTGCCCTcc                                                                                                >  1:3941816/1‑100 (MQ=255)
      ccccGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCACCg                                                                                                >  1:4016763/1‑101 (MQ=255)
        ccGTCGCCAGCCCCCGGGTCTGAAAGCGGAACGGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGc                                                                                              <  1:1132194/101‑1 (MQ=255)
          gTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCtt                                                                                            >  2:2303305/1‑101 (MQ=255)
            cGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCtttt                                                                                          >  1:4076992/1‑99 (MQ=255)
              ccAGCCCCCGGGTCTGAAAGCGGAACGGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTgccg                                                                                        <  1:526421/101‑1 (MQ=255)
               cAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGGGGCATCGGccttctgc                                                                                       >  2:773796/1‑94 (MQ=255)
               cAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGGGCCCTCGGCttttcgc                                                                                       >  1:3157411/1‑96 (MQ=255)
                aGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTttcgcc                                                                                      >  2:2279900/1‑101 (MQ=255)
                 gCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCttttccccc                                                                                     >  1:3407096/1‑94 (MQ=255)
                 gCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCttttccccc                                                                                     >  2:4045554/1‑94 (MQ=255)
                   ccccGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATc                                                                                   <  2:4422042/100‑1 (MQ=255)
                   aCCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGGGCCATCGGCttttccccccc                                                                                   >  1:4325104/1‑92 (MQ=255)
                    ccggggTCTAAAGGCGGAACTGCGGCCACGGCGTTCCCAGCGCATAAGGCGTGGTGATCCCCTTC.GGGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATcg                                                                                  <  1:1081649/98‑1 (MQ=255)
                    cccGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATcg                                                                                  <  1:3888913/101‑1 (MQ=255)
                      cggggatGAAAGCGAAGCGGCGGCCCCAGCGTTTCAAGCGAATAAAGCGTGGTGATCGCCTTG.GCGGTGGAGGC.GGGCGCCATCGGCTTGCCGCCATcgcg                                                                                <  2:1676639/95‑1 (MQ=255)
                           cttaaaGGGGAACTGCGGCCCCAGCTTTGCCAGCGCATAAAGCGTGGTGGTCGCCTTC.GCGGTCGAGGG.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCt                                                                           <  1:992852/98‑1 (MQ=255)
                           cTGAAAGCGGAACGGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTACCGCCATCGCGCGCCt                                                                           <  2:806818/101‑1 (MQ=255)
                            tGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGGGCCCTCGGCTTTCCGCCCCCGCCCGcccc                                                                          >  2:886815/1‑99 (MQ=255)
                            tGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTc                                                                          <  2:4148268/101‑1 (MQ=255)
                             gAAAGCGGACCTGCGGCCCCAGCTTTTCCAGCGCAAAAAGGGGGGTGACCGCCTTC.GCGGTCGGGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTcc                                                                         <  2:154955/101‑1 (MQ=255)
                             aaaaGCGAAACGGCGGCCCCAGCTTTTCCAGCGCATAAGGCGTGGTGATCGCCTTC.GGGGTCGAGGG.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTcc                                                                         <  2:4161682/100‑1 (MQ=255)
                              aaaGCGGAACTGGGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGGCGGGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCa                                                                        <  1:626297/101‑1 (MQ=255)
                                aGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTTCGCCCCCCCGCGCCCccccc                                                                      >  1:647457/1‑98 (MQ=255)
                                    gAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCa                                                                  <  1:3922050/101‑1 (MQ=255)
                                     aaCCGCGGCCCCAGCGTTTCCAGCGAATAAAGCGTGGTGATCGCCTTC.GCGGTCGGGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAg                                                                 <  1:4031716/101‑1 (MQ=255)
                                        tGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTTCGCCCTCCCCCGCCCCCCGCCccccccc                                                              >  1:1360202/1‑96 (MQ=255)
                                        tGCGGCCCCAGCGTTTCCAGCGCAAAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGccc                                                              <  2:226515/101‑1 (MQ=255)
                                        tGCGCCCCCAGCTTTTCCAGCGCATAAAGCGTGGTGATCCCCTCC.GCGGTCGAGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGccc                                                              <  2:3762804/101‑1 (MQ=255)
                                            gCCCCGGCGTTTCCGGCGCATAAGGCGTGGTGATCCCCTTC.GCGGTCGGGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTg                                                          <  2:2808066/101‑1 (MQ=255)
                                            gCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGGGCCATCGGCTTTCCCCCCCCCCGCCCCCCCCGCAcccaacccccc                                                          >  2:2819661/1‑92 (MQ=255)
                                            cccccAGCTTTTCCAGCGCATAAAGCGTGGTGATCCCCTTC.GCGGTCGAGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTg                                                          <  1:1098476/100‑1 (MQ=255)
                                            cccccAGCGTGCCCAGCGCAAAAAGCGTGGTGATCCCCTTC.GCGGTCGAGGG.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTg                                                          <  1:3339632/100‑1 (MQ=255)
                                              cccCGCGTTCCCAGCGAATAAAGCGGGGTGCTCCCCTGC.GCGGTCGGGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTgcg                                                        <  2:1455384/101‑1 (MQ=255)
                                              cccAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTgcg                                                        <  2:2668300/101‑1 (MQ=255)
                                                  gCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTTCCCCATCGCGCGGCCCCCGCACCAGCCCCCCgcgggcc                                                    >  1:1160006/1‑100 (MQ=255)
                                                   cGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTCCCGCCCCCCCCGCCTCCCCCACCCACCCCCCgcgcgccc                                                   >  2:507859/1‑99 (MQ=255)
                                                   cGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTCCCCCCCCTCGCCCCCCCCCCCCCCCCCCCCTgcgcgcct                                                   >  1:1211108/1‑99 (MQ=255)
                                                     tttCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCg                                                 <  1:1384793/101‑1 (MQ=255)
                                                     tttCCAGCGCATAAAGCGTGGTGACCCCCTTC.GCGGTCGGGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCg                                                 <  1:1700913/101‑1 (MQ=255)
                                                      tgccAGCGCAGAAAGCGTGGTGCTCGCCTTC.GGGGTCGGGGG.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCgg                                                <  2:281619/99‑1 (MQ=255)
                                                        ccAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGcc                                              <  2:4076732/101‑1 (MQ=255)
                                                         cAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTTCCCCCCCGCGCGCCTCCCGCACCCGCCCCCCccgcgccacgggcc                                             >  1:4364612/1‑93 (MQ=255)
                                                         cAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGcca                                             <  2:3157187/101‑1 (MQ=255)
                                                         cAGCGCAAAAGGCGTGGTGGTC.CCTTCGGGGGGGGAGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGcca                                             <  2:2814289/101‑1 (MQ=255)
                                                           gcgcATAAGGCGGGGTGCCCCCCTCG.GGGGCGGAGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGccacc                                           <  2:819171/101‑1 (MQ=255)
                                                            ggaagaaggggtggtgATCCCGTCG.GCGGTCTAGCG.GGCCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGccacca                                          <  1:326367/91‑1 (MQ=255)
                                                             gcAAAAAGGGTGGTGATCCCCTTC.GCGGTCGAGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGTATCGGCcaccac                                         <  1:2427007/101‑1 (MQ=255)
                                                               aTAAAGCGTGTTGCTCGCCTCG.GCGG.CGGGGCGGGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACAt                                       <  2:4211577/101‑1 (MQ=255)
                                                               aTAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTTCCCCCACCCCCGCCCCCCCCACCAACCCCCCGCCCGCCTCGGCCCCcccaa                                       >  1:2500271/1‑100 (MQ=255)
                                                                tAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATa                                      <  1:4089495/101‑1 (MQ=255)
                                                                 aaggCGTGGTGACCCCCTTC.GCGGTCGAGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAg                                     <  2:4362960/98‑1 (MQ=255)
                                                                 aaaGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTTCCCCCTCCCCCGCCCCCCCCCCCCCCCCCCCGCGCGCCCCCGCCCCCACATaa                                     >  2:2286310/1‑100 (MQ=255)
                                                                  aggCGTGGTGATCGCCTCG.GGGGCGGGGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGc                                    <  1:4046674/99‑1 (MQ=255)
                                                                  aaGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGc                                    <  1:979241/101‑1 (MQ=255)
                                                                   ggggtggggACCGCCTTC.GGGGGGGGGGG.GGCCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGcc                                   <  1:1047073/98‑1 (MQ=255)
                                                                   aGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTCCCCCCCCCCCCGCCCCCCCCACCCCCCCGCCGCGCGCCCCGGGCACcccaaaacc                                   >  2:2911552/1‑96 (MQ=255)
                                                                    gCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCg                                  <  1:994419/101‑1 (MQ=255)
                                                                      gtggtgATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGAc                                <  1:2280045/101‑1 (MQ=255)
                                                                      gtggtgATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGAc                                <  1:3403257/101‑1 (MQ=255)
                                                                         gtgATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATc                             <  1:181344/101‑1 (MQ=255)
                                                                         gtgATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATc                             <  2:3406859/101‑1 (MQ=255)
                                                                          gacccGCCTGC.GCGGGGGGGGG.GGCCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATcg                            <  2:4165256/97‑1 (MQ=255)
                                                                             tCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATcgccg                         <  2:1008470/101‑1 (MQ=255)
                                                                             tCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATcgccg                         <  2:2206718/101‑1 (MQ=255)
                                                                              cGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTCCCCCCTCCCGCGCCCCCCGCACCCCCCCCCTGCCCGCCTCGGCCCCCCCAAAACCGACAAcgcccc                        >  1:2221184/1‑99 (MQ=255)
                                                                                cttgc.gcgGTGGGGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGccc                      <  2:3151135/97‑1 (MQ=255)
                                                                                ccTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTTCCCCCCCCCCGCGCCCCCCGCACCAGCCCCCCGCGCGCCTCCGCCCCCCCATAGCCGACAACCCCGccc                      >  2:993987/1‑101 (MQ=255)
                                                                                 cTTC.GGGGTCGGGGC.GGGCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCa                     <  1:3733159/101‑1 (MQ=255)
                                                                                 cTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCa                     <  1:1355907/101‑1 (MQ=255)
                                                                                 cTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCa                     <  2:1012988/101‑1 (MQ=255)
                                                                                  ttC.GCGGTCGAGGC.GGGCGCCATCGGCTTTCCGCCATCCCGCGCCTCCCGCACCACCCCCCCGCGCGCCTCGGCCCCCACACAGACGACATCGCCGccccc                    >  2:27071/1‑99 (MQ=255)
                                                                                  ttC.GCGGTCGAGGC.GGGCGCCATCGGCTTTCCCCCCTCCCGCGCCCCCCCCCCCAGCCCGCCGCGCGCCTCCGGCACCCCATAGCCCACAACCCCGccccc                    >  1:2138125/1‑99 (MQ=255)
                                                                                  ttC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAg                    <  2:2964395/101‑1 (MQ=255)
                                                                                    c.gcgGTCGAGGC.GGGCGCCATCGGCTTTCCGCCATCGCGCGCCTCCCGCACCAACCCCCTGCGCGCCTCCGCCCCCACATAGCCGACATCGCCCccccccc                  >  2:3902063/1‑97 (MQ=255)
                                                                                    c.gcgGGCGGGGC.GGCCCCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAgcc                  <  1:2834842/101‑1 (MQ=255)
                                                                                      gcgGTCGAGGC.GGGCGCCATCGGCTTTCCCCCCTCCCGCCCCCCCCCCCCCCCCCCCCCGCCCGCGTCCGCCCCCCCCTACCCCACATCGCCGcccccccc                 >  1:3148222/1‑96 (MQ=255)
                                                                                       cgGTCGAGGC.GGGCGCCATCGGCTTTCCGCCCTCGCGCGCCCCCCGCACCAACCCCCTGCGCGCCTCGGCCCCCACATAGCCGACATCGCCGCCCCgccgc                >  2:3473048/1‑101 (MQ=255)
                                                                                          tCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCCCCACCACCAGCCCGCCGCGCGCATCGGCCACCACATAACCGACATCCCCCCCCAgccccgcc             >  2:1046290/1‑101 (MQ=255)
                                                                                                c.gggcgCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCcgcg       <  2:4031672/101‑1 (MQ=255)
                                                                                                    gcgcCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGAta    <  2:1357137/101‑2 (MQ=255)
                                                                                                    gcgcCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATc    >  2:4078766/1‑101 (MQ=255)
                                                                                                    gcgcCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATc    <  1:3430615/101‑1 (MQ=255)
                                                                                                      gcCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATCAg  <  1:1491873/101‑1 (MQ=255)
                                                                                                      gcCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCAGACATCGCCGCCCAGCCGCCCCGCCGCGATCAg  >  1:3829887/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GACCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGGTGATCGCCTTC.GCGGTCGAGGC.GGGCGCCATCGGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATCAG  >  NC_014034/2819568‑2819768

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG