Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,743 G→A 3.0% intergenic (+86/‑74) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7430G→A 3.0% 9.6 ‑1.4 ‑0.0233intergenic (+86/‑74)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (130/96):  total (136/97)
Fisher's exact test strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.68e-01

Only 100 of 247 total aligned reads displayed.

AACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCC  >  NC_014034/2950644‑2950843
                                                                                                    |                                                                                                     
  cTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCTCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gggg                                                                                                    >  1:2903928/1‑101 (MQ=255)
  cTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gggg                                                                                                    <  2:3725897/101‑1 (MQ=255)
      aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGa                                                                                                <  1:5400/101‑1 (MQ=255)
       tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                                                               >  2:2323821/1‑101 (MQ=255)
         tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                                                             <  1:275239/101‑1 (MQ=255)
          cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                                                            <  1:4056947/101‑1 (MQ=255)
              agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                                                        >  2:2923968/1‑101 (MQ=255)
                caagcCGGGGGCTCTGCCCCCGGACCCCCGGGGAATTTTAGGGAAGAAGAAACAAACCCCCTTCATTTTTCCCCCAAAAACCC.GGGGGGGAATTTGCCGTc                                                                                      >  1:801725/1‑101 (MQ=16)
                 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                     >  2:2959900/1‑101 (MQ=255)
                 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                     >  1:571000/1‑101 (MQ=255)
                 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                     <  2:2271214/101‑1 (MQ=255)
                  agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                                                    >  1:3260598/1‑101 (MQ=255)
                   gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                                   <  2:2870605/101‑1 (MQ=255)
                      gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCACGATGAAACACGCCCCTTTCATCTTGCCCCAAATATACC.GGGGGTNAATTTGcnnnnnnnnnn                                                                                >  2:506375/1‑91 (MQ=21)
                       ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCaaa                                                                               >  2:2291755/1‑101 (MQ=255)
                       ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCaaa                                                                               >  2:3190467/1‑101 (MQ=255)
                          gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.gag                                                                           >  1:436214/1‑101 (MQ=255)
                           ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgg                                                                          >  2:2741234/1‑101 (MQ=255)
                           ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgg                                                                          >  2:3367840/1‑101 (MQ=255)
                             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                        >  2:3331107/1‑101 (MQ=255)
                             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                        <  1:1007239/101‑1 (MQ=255)
                             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                        <  1:1270668/101‑1 (MQ=255)
                              tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAggggg                                                                       <  2:526958/101‑1 (MQ=255)
                              tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAggggg                                                                       <  2:137675/101‑1 (MQ=255)
                              tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAggggg                                                                       >  1:229634/1‑101 (MQ=255)
                               ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggc                                                                     >  2:203506/9‑99 (MQ=25)
                               gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGc                                                                      >  2:3922031/1‑101 (MQ=255)
                                cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCa                                                                     <  2:4049646/101‑1 (MQ=255)
                                 cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggcag                                                                   >  1:3630854/7‑97 (MQ=25)
                                  cccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGc                                                                   <  2:1522271/101‑1 (MQ=255)
                                   ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCt                                                                  <  1:3584449/101‑1 (MQ=255)
                                    cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTc                                                                 >  2:27589/1‑101 (MQ=255)
                                     ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTcc                                                                >  2:3453771/1‑101 (MQ=255)
                                     ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccc                                                               >  1:1123263/4‑101 (MQ=2)
                                     ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccc                                                               >  2:2565537/4‑101 (MQ=2)
                                      gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTccc                                                               <  2:866259/101‑1 (MQ=255)
                                       aCCCCCGGGATATTTGAGGCAAGATGAAAAACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAA.GAGGGGGGAGCTcccc                                                              >  1:3720201/1‑101 (MQ=255)
                                        cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTccccc                                                             >  1:3916675/1‑101 (MQ=255)
                                         ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                            <  1:3987408/101‑1 (MQ=255)
                                          cccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTg                                                           >  1:1946464/1‑101 (MQ=255)
                                           ccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGc                                                          <  2:1715538/101‑1 (MQ=255)
                                             gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGccc                                                        >  2:2147759/1‑101 (MQ=255)
                                               gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCg                                                      >  1:3485654/1‑101 (MQ=255)
                                                 tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGc                                                    >  2:1121080/1‑101 (MQ=255)
                                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                   <  2:693440/101‑1 (MQ=255)
                                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTACCCCTGCCCCTGCt                                                   >  2:2974277/1‑101 (MQ=255)
                                                    ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTga                                                 >  2:3525354/1‑101 (MQ=255)
                                                     tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                <  2:837273/101‑1 (MQ=255)
                                                      gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                               <  1:4101697/101‑1 (MQ=255)
                                                       aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                              <  2:4127088/101‑1 (MQ=255)
                                                         gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                            <  2:1871434/101‑1 (MQ=255)
                                                          cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg                                           >  1:383727/1‑101 (MQ=255)
                                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                          >  2:3636010/1‑101 (MQ=255)
                                                            aGATGAAACACGCCCCTTTCATCTCGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTTAGGGAGGCg                                         >  1:4263551/1‑101 (MQ=255)
                                                              aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                       >  2:1195722/1‑101 (MQ=255)
                                                                gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                     <  1:1895270/101‑1 (MQ=255)
                                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                  >  2:4270238/1‑101 (MQ=255)
                                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                  >  2:2848116/1‑101 (MQ=255)
                                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                  <  1:1494911/101‑1 (MQ=255)
                                                                     acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                                >  2:4313028/1‑101 (MQ=255)
                                                                      cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                               <  2:1303437/101‑1 (MQ=255)
                                                                       gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                              >  1:2531259/1‑101 (MQ=255)
                                                                       gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                              >  1:2501709/1‑101 (MQ=255)
                                                                       gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                              >  1:2812503/1‑101 (MQ=255)
                                                                       gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                              >  1:2304042/1‑101 (MQ=255)
                                                                       gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                              >  2:1614927/1‑101 (MQ=255)
                                                                         cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                            <  1:2564296/101‑1 (MQ=255)
                                                                         cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                            >  1:1744219/1‑101 (MQ=255)
                                                                         cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                            <  1:2882388/101‑1 (MQ=255)
                                                                          ccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCtt                           <  1:1309466/101‑1 (MQ=255)
                                                                           cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                          <  2:3055319/101‑1 (MQ=255)
                                                                           cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                          <  1:3968681/101‑1 (MQ=255)
                                                                            tttCATCTTGCGCCAAATGTCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                         <  1:1843979/101‑1 (MQ=255)
                                                                            tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                         <  2:2523152/101‑1 (MQ=255)
                                                                              tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                       >  2:156633/1‑101 (MQ=255)
                                                                                aTCTTGCCCCAACTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                     >  1:2425041/1‑101 (MQ=255)
                                                                                aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCTGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                     <  1:3820254/101‑1 (MQ=255)
                                                                                aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                     <  1:2038716/101‑1 (MQ=255)
                                                                                 tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                    >  1:1698264/1‑101 (MQ=255)
                                                                                 tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                    >  2:3225209/1‑101 (MQ=255)
                                                                                   ctcccccAAATCGCGC.GGGGGTGAGTTTCGCGGGAGGAAAAGGAGGGGGCAGCCCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                   <  1:3359859/98‑1 (MQ=255)
                                                                                    tGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                 >  1:2169658/1‑101 (MQ=255)
                                                                                      ccccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt               >  1:1597132/1‑101 (MQ=255)
                                                                                         cAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt            >  1:3109538/1‑101 (MQ=255)
                                                                                          aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc           >  1:268713/1‑101 (MQ=255)
                                                                                          aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc           >  1:309667/1‑101 (MQ=255)
                                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg          <  1:518348/101‑1 (MQ=255)
                                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg          <  1:2722495/101‑1 (MQ=255)
                                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg          >  1:2225638/1‑101 (MQ=255)
                                                                                             tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa        <  2:1834073/101‑1 (MQ=255)
                                                                                             tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa        >  2:677908/1‑101 (MQ=255)
                                                                                             tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa        >  1:1320797/1‑101 (MQ=255)
                                                                                              atCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag       >  1:1131607/1‑101 (MQ=255)
                                                                                               tCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga      <  1:3302026/101‑1 (MQ=255)
                                                                                                ccc.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag     <  2:2636837/101‑1 (MQ=255)
                                                                                                  c.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc   >  2:2513685/1‑101 (MQ=255)
                                                                                                  c.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc   <  1:389740/101‑1 (MQ=255)
                                                                                                    gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc  <  1:960467/101‑1 (MQ=255)
                                                                                                    gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc  >  1:301030/1‑101 (MQ=255)
                                                                                                    gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc  <  1:2657712/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
AACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCC  >  NC_014034/2950644‑2950843

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG