Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,754 T→G 4.6% intergenic (+97/‑63) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7540T→G 4.6% 20.1 ‑2.1 ‑0.0241intergenic (+97/‑63)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (9/2):  ref base (129/101):  total (138/103)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.75e-01

Only 100 of 260 total aligned reads displayed.

CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGG  >  NC_014034/2950654‑2950854
                                                                                                     |                                                                                                     
cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                                                                       <  1:4056947/101‑1 (MQ=255)
  gAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGACTTTGc                                                                                                     >  2:32811/1‑101 (MQ=255)
   aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGcc                                                                                                    <  1:2677897/101‑1 (MQ=255)
    agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                                                                   >  2:2923968/1‑101 (MQ=255)
     gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGt                                                                                                  >  2:1387372/1‑101 (MQ=255)
      caagcCGGGGGCTCTGCCCCCGGACCCCCGGGGAATTTTAGGGAAGAAGAAACAAACCCCCTTCATTTTTCCCCCAAAAACCC.GGGGGGGAATTTGCCGTc                                                                                                 >  1:801725/1‑101 (MQ=16)
      caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                                                                 >  1:2469793/1‑101 (MQ=255)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                                >  2:2959900/1‑101 (MQ=255)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                                <  2:2271214/101‑1 (MQ=255)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                                >  1:571000/1‑101 (MQ=255)
        agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                                                               >  2:2459004/1‑101 (MQ=255)
        agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                                                               <  1:3926197/101‑1 (MQ=255)
         gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                                              <  2:2870605/101‑1 (MQ=255)
         gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                                              <  2:3636053/101‑1 (MQ=255)
          ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                                                             >  1:2637008/1‑101 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                                            >  1:1190841/1‑101 (MQ=255)
             ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCaaa                                                                                          >  2:3190467/1‑101 (MQ=255)
              gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.g                                                                                        <  1:521965/101‑1 (MQ=255)
                 ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GCgg                                                                                     <  1:2558843/101‑1 (MQ=255)
                 ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgg                                                                                     >  2:3367840/1‑101 (MQ=255)
                  tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTACCGTCAGGCAAA.GAggg                                                                                    >  2:4007673/1‑101 (MQ=255)
                   ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                   >  2:2706342/1‑101 (MQ=255)
                   ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                   <  1:1270668/101‑1 (MQ=255)
                   ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                   <  1:1007239/101‑1 (MQ=255)
                     ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggc                                                                                >  2:203506/9‑99 (MQ=25)
                     gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGc                                                                                 >  2:3922031/1‑101 (MQ=255)
                       cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggcag                                                                              >  1:3630854/7‑97 (MQ=25)
                        cccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGc                                                                              >  2:3789619/1‑101 (MQ=255)
                         ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCt                                                                             <  1:3584449/101‑1 (MQ=255)
                         ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCt                                                                             >  2:2782646/1‑101 (MQ=255)
                          cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTc                                                                            >  2:27589/1‑101 (MQ=255)
                           ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTcc                                                                           >  2:3453771/1‑101 (MQ=255)
                           ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccc                                                                          >  1:184501/4‑101 (MQ=2)
                           ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccc                                                                          >  2:2565537/4‑101 (MQ=2)
                           ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccc                                                                          >  1:1123263/4‑101 (MQ=2)
                            gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTccc                                                                          >  2:518296/1‑101 (MQ=255)
                             aCCCCCGGGATATTTGAGGCAAGATGAAAAACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAA.GAGGGGGGAGCTcccc                                                                         >  1:3720201/1‑101 (MQ=255)
                               ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                       <  1:3987408/101‑1 (MQ=255)
                                 ccGGGATATTTGAGGCAAGATGAAACACGCCGCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGc                                                                     >  1:3955816/1‑101 (MQ=255)
                                 ccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGc                                                                     <  2:1715538/101‑1 (MQ=255)
                                    ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcccc                                                                  >  1:2883561/1‑101 (MQ=255)
                                     gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCg                                                                 >  1:3485654/1‑101 (MQ=255)
                                      atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCgg                                                                >  1:3281819/1‑101 (MQ=255)
                                       tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGc                                                               >  2:1121080/1‑101 (MQ=255)
                                       tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGc                                                               >  1:3860409/1‑101 (MQ=255)
                                        atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                              <  2:693440/101‑1 (MQ=255)
                                        atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                              >  1:2023386/1‑101 (MQ=255)
                                        atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                              <  1:2939678/101‑1 (MQ=255)
                                        atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTACCCCTGCCCCTGCt                                                              >  2:2974277/1‑101 (MQ=255)
                                          ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTga                                                            >  2:3525354/1‑101 (MQ=255)
                                           tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                           <  2:837273/101‑1 (MQ=255)
                                            gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                                          <  1:4101697/101‑1 (MQ=255)
                                             aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                                         <  2:4127088/101‑1 (MQ=255)
                                              ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                        <  1:223729/101‑1 (MQ=255)
                                              ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                        <  1:27602/101‑1 (MQ=255)
                                               gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                       <  2:1871434/101‑1 (MQ=255)
                                                 aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                     >  1:4436752/1‑101 (MQ=255)
                                                  aGATGAAACACGCCCCTTTCATCTCGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTTAGGGAGGCg                                                    >  1:4263551/1‑101 (MQ=255)
                                                     tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                 <  2:2206655/101‑1 (MQ=255)
                                                     tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                 >  1:2719855/1‑101 (MQ=255)
                                                      gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                <  1:1895270/101‑1 (MQ=255)
                                                         acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                             <  1:1494911/101‑1 (MQ=255)
                                                         acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                             >  2:4270238/1‑101 (MQ=255)
                                                          cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTTGa                                            >  2:1407527/1‑101 (MQ=255)
                                                           acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                                           >  2:4313028/1‑101 (MQ=255)
                                                            cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                          >  2:2321847/1‑101 (MQ=255)
                                                            cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                          <  2:1303437/101‑1 (MQ=255)
                                                             gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                         >  1:2812503/1‑101 (MQ=255)
                                                             gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                         >  1:2304042/1‑101 (MQ=255)
                                                             gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                         >  2:1614927/1‑101 (MQ=255)
                                                               cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                                       >  1:1744219/1‑101 (MQ=255)
                                                               cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                                       <  1:1984491/101‑1 (MQ=255)
                                                                 cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                     <  2:801643/101‑1 (MQ=255)
                                                                 cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                     <  2:3055319/101‑1 (MQ=255)
                                                                  tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                    <  2:2523152/101‑1 (MQ=255)
                                                                    tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                  >  2:156633/1‑101 (MQ=255)
                                                                    tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                  >  1:1309551/1‑101 (MQ=255)
                                                                      aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCTGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                                <  1:3820254/101‑1 (MQ=255)
                                                                       tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                               >  1:1698264/1‑101 (MQ=255)
                                                                        cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                              >  2:1269154/1‑101 (MQ=255)
                                                                         ctcccccAAATCGCGC.GGGGGTGAGTTTCGCGGGAGGAAAAGGAGGGGGCAGCCCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                              <  1:3359859/98‑1 (MQ=255)
                                                                          tGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                            >  1:2169658/1‑101 (MQ=255)
                                                                            ccccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                          >  1:1597132/1‑101 (MQ=255)
                                                                               cAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                       >  1:3109538/1‑101 (MQ=255)
                                                                                aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                      >  1:309667/1‑101 (MQ=255)
                                                                                 aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                     >  2:866515/1‑101 (MQ=255)
                                                                                   tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                   <  2:1834073/101‑1 (MQ=255)
                                                                                   tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                   >  2:677908/1‑101 (MQ=255)
                                                                                   tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                   >  1:2405344/1‑101 (MQ=255)
                                                                                    atCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                  >  2:615602/1‑101 (MQ=255)
                                                                                    atCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                  >  1:3022473/1‑101 (MQ=255)
                                                                                       cc.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAgg               >  1:2526794/1‑101 (MQ=255)
                                                                                        c.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc              <  1:389740/101‑1 (MQ=255)
                                                                                          gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             <  1:960467/101‑1 (MQ=255)
                                                                                           ggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc            <  1:1387469/101‑1 (MQ=255)
                                                                                           ggggTAATTTTGCCCGCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc            <  2:4436480/101‑1 (MQ=255)
                                                                                                  aTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt     >  2:3243352/1‑101 (MQ=255)
                                                                                                   tttGCGGTCAGGAAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc    <  1:2213266/101‑1 (MQ=255)
                                                                                                   tttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc    >  1:3196988/1‑101 (MQ=255)
                                                                                                   tttGCCGCAAGGAAAG.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc    <  1:4313297/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGG  >  NC_014034/2950654‑2950854

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG