Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,760 C→A 4.6% intergenic (+103/‑57) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7600C→A 4.6% 19.0 ‑2.1 ‑0.0240intergenic (+103/‑57)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (9/2):  ref base (126/102):  total (136/104)
Fisher's exact test strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.60e-01

Only 100 of 256 total aligned reads displayed.

CAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGC  >  NC_014034/2950660‑2950860
                                                                                                    |                                                                                                    
caagcCGGGGGCTCTGCCCCCGGACCCCCGGGGAATTTTAGGGAAGAAGAAACAAACCCCCTTCATTTTTCCCCCAAAAACCCGGGGGGGAATTTGCCGTc                                                                                                      >  1:801725/1‑101 (MQ=16)
caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTc                                                                                                      <  2:3722989/101‑1 (MQ=255)
 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCa                                                                                                     >  2:2959900/1‑101 (MQ=255)
  agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAg                                                                                                    >  1:3260598/1‑101 (MQ=255)
  agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAg                                                                                                    >  2:2459004/1‑101 (MQ=255)
    ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGc                                                                                                  >  1:2637008/1‑101 (MQ=255)
     cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATTAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCa                                                                                                 <  1:3302808/101‑1 (MQ=255)
     cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCa                                                                                                 >  1:1190841/1‑101 (MQ=255)
     cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGCGAATTTGCCGTCAGGCa                                                                                                 >  2:4272915/1‑101 (MQ=255)
       ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCaaa                                                                                               <  1:2301410/101‑1 (MQ=255)
       ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTGCCGTCAGGCaaa                                                                                               >  2:2291755/1‑101 (MQ=255)
        gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAag                                                                                              <  1:3496077/101‑1 (MQ=255)
          gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAagag                                                                                            >  1:436214/1‑101 (MQ=255)
           ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGCgg                                                                                           <  1:2558843/101‑1 (MQ=255)
             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                                                                         <  1:1270668/101‑1 (MQ=255)
             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                                                                         <  1:1007239/101‑1 (MQ=255)
              tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAggggg                                                                                        <  2:526958/101‑1 (MQ=255)
               gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGc                                                                                       >  2:3922031/1‑101 (MQ=255)
                cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                                                                      <  2:4049646/101‑1 (MQ=255)
                  tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcg                                                                                   >  1:2095521/7‑100 (MQ=2)
                  cccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGc                                                                                    >  1:70987/1‑101 (MQ=255)
                    cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTc                                                                                  <  2:2988177/101‑1 (MQ=255)
                    cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTc                                                                                  >  1:2206794/1‑101 (MQ=255)
                     tgccccccGGGATAGTTGAGGCAATATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcc                                                                                 <  2:1286966/98‑1 (MQ=255)
                     ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcc                                                                                 >  2:131803/1‑101 (MQ=255)
                     ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGCGccc                                                                                >  2:2745562/4‑101 (MQ=2)
                     ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGCGccc                                                                                >  1:1123263/4‑101 (MQ=2)
                      gccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTAAATTGGCC.AAAGGCCAAGAGGGGGCAGCGcccc                                                                               <  2:349972/99‑1 (MQ=0)
                       aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcccc                                                                               >  2:2894590/1‑101 (MQ=255)
                       aCCCCCGGGATATTTGAGGCAAGATGAAAAACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTGCCGTCAGGCAAAGAGGGGGGAGCTcccc                                                                               >  1:3720201/1‑101 (MQ=255)
                        cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccccc                                                                              >  1:3916675/1‑101 (MQ=255)
                          cccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTg                                                                            >  1:1946464/1‑101 (MQ=255)
                           ccGGGATATTTGAGGCAAGATGAAACACGCCGCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGc                                                                           >  1:3955816/1‑101 (MQ=255)
                             gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGccc                                                                         >  2:2147759/1‑101 (MQ=255)
                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcccc                                                                        >  1:2883561/1‑101 (MQ=255)
                                atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                                                                      <  1:563217/101‑1 (MQ=255)
                                 tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                                                                     >  2:3359622/1‑101 (MQ=255)
                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                                                    <  1:2939678/101‑1 (MQ=255)
                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                                                    <  2:693440/101‑1 (MQ=255)
                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                                                    >  1:2023386/1‑101 (MQ=255)
                                   tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                                                                   <  2:2858874/101‑1 (MQ=255)
                                    ttGAGGCAAGATGAAACACGCCCCTTTTATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                                                                  >  2:3130977/1‑101 (MQ=255)
                                     tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                                 <  2:837273/101‑1 (MQ=255)
                                      gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTGAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                                                >  1:3098763/1‑101 (MQ=255)
                                      gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                                                <  1:4101697/101‑1 (MQ=255)
                                       aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                                               <  2:1727210/101‑1 (MQ=255)
                                        ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                              <  1:27602/101‑1 (MQ=255)
                                         gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                             <  2:1871434/101‑1 (MQ=255)
                                          cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg                                                            >  1:383727/1‑101 (MQ=255)
                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                           >  2:3636010/1‑101 (MQ=255)
                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                           >  2:2213127/1‑101 (MQ=255)
                                             gATGAAACACGCCCCTTTCATCTTGCCCCAACTATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                                         >  1:3979666/1‑101 (MQ=255)
                                             gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                                         >  2:3439160/1‑101 (MQ=255)
                                              aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                                        >  2:2770461/1‑101 (MQ=255)
                                               tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                       <  1:4019300/101‑1 (MQ=255)
                                               tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                       >  1:2719855/1‑101 (MQ=255)
                                                gAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                      <  1:1895270/101‑1 (MQ=255)
                                                gAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                      <  2:1795377/101‑1 (MQ=255)
                                                  aaCACGCCCCTTTCATCTTGCCCCAAATATCCCAGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg                                                    <  1:3789872/101‑1 (MQ=255)
                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                                   <  1:1494911/101‑1 (MQ=255)
                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                                   >  1:388111/1‑101 (MQ=255)
                                                    cacGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTTGa                                                  >  2:1407527/1‑101 (MQ=255)
                                                    cacGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                                  <  2:1128831/101‑1 (MQ=255)
                                                     acGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                                                 >  2:2550835/1‑101 (MQ=255)
                                                       gCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                               >  2:1614927/1‑101 (MQ=255)
                                                       gCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                               >  1:2812503/1‑101 (MQ=255)
                                                         cccTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                                             >  1:1744219/1‑101 (MQ=255)
                                                           cTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                           <  2:801643/101‑1 (MQ=255)
                                                           cTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                           >  2:3200663/1‑101 (MQ=255)
                                                            tttCATCTTGCGCCAAATGTCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                          <  1:1843979/101‑1 (MQ=255)
                                                             ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                         >  2:2516508/1‑101 (MQ=255)
                                                              tCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                        >  2:156633/1‑101 (MQ=255)
                                                              tCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                        >  1:1309551/1‑101 (MQ=255)
                                                               cATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                       >  2:2012231/1‑101 (MQ=255)
                                                                 tCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                     >  1:1698264/1‑101 (MQ=255)
                                                                 tCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                     >  1:315612/1‑101 (MQ=255)
                                                                     gCCCCAAATATCCGGGGGGTGAATTTGCC.TCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                                <  2:1065574/101‑1 (MQ=255)
                                                                          aaaTATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                            >  1:268713/1‑101 (MQ=255)
                                                                           aaTATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                           >  2:866515/1‑101 (MQ=255)
                                                                           aaTATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                           <  1:518348/101‑1 (MQ=255)
                                                                           aaTATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                           >  1:2225638/1‑101 (MQ=255)
                                                                            atatCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                          <  1:1579145/101‑1 (MQ=255)
                                                                             tatCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                         >  1:1320797/1‑101 (MQ=255)
                                                                             tatCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                         >  1:2405344/1‑101 (MQ=255)
                                                                             tatCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                         >  2:677908/1‑101 (MQ=255)
                                                                              atCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                        >  1:3385467/1‑101 (MQ=255)
                                                                              atCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                        >  2:615602/1‑101 (MQ=255)
                                                                                cccGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                      <  2:2636837/101‑1 (MQ=255)
                                                                                 cggggggTGAATTTCCCGACAGGAAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTTAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAgg                     <  2:4263282/99‑1 (MQ=255)
                                                                                 ccGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAgg                     >  1:2526794/1‑101 (MQ=255)
                                                                                   gggggTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                   >  1:301030/1‑101 (MQ=255)
                                                                                     gggTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcg                 <  2:4335160/101‑1 (MQ=255)
                                                                                       gTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcg               <  1:4273184/101‑1 (MQ=255)
                                                                                         gAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg             <  1:2459161/101‑1 (MQ=255)
                                                                                           aTTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt           >  2:3243352/1‑101 (MQ=255)
                                                                                            tttGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc          >  2:380917/1‑101 (MQ=255)
                                                                                            tttGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc          >  1:3628871/1‑101 (MQ=255)
                                                                                              tGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCgg        <  1:3064363/101‑1 (MQ=255)
                                                                                                    cAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGc  >  2:4120123/1‑101 (MQ=255)
                                                                                                    cAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGc  <  1:4170586/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGC  >  NC_014034/2950660‑2950860

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG