Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,959,195 T→C 100% E74E (GAA→GAG RCAP_rcc02771 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,959,1950T→C100.0% 806.4 210E74E (GAA→GAGRCAP_rcc02771TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (103/107):  ref base (0/0):  total (103/107)

Only 100 of 219 total aligned reads displayed.

AGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCG  >  NC_014034/2959097‑2959292
                                                                                                  |                                                                                                 
aGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTc                                                                                                 >  2:754261/1‑101 (MQ=255)
aGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTc                                                                                                 <  1:144529/101‑1 (MQ=255)
  ccccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCAt                                                                                               >  1:3482194/1‑101 (MQ=255)
  ccccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCAt                                                                                               <  2:2935628/101‑1 (MQ=255)
  acccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCAt                                                                                               <  1:2073895/100‑1 (MQ=255)
   cccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATc                                                                                              <  1:2152943/101‑1 (MQ=255)
   cccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATc                                                                                              >  2:1248531/1‑101 (MQ=255)
    ccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTAATCg                                                                                             >  2:1055553/1‑101 (MQ=255)
     cAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCgg                                                                                            <  1:1809995/101‑1 (MQ=255)
     cAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCgg                                                                                            <  2:1132525/101‑1 (MQ=255)
      aGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGc                                                                                           >  2:3787880/1‑101 (MQ=255)
       gcggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGcc                                                                                          <  1:2372450/101‑1 (MQ=255)
        cggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCt                                                                                         <  1:3268342/101‑1 (MQ=255)
         ggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTg                                                                                        <  1:3731136/101‑1 (MQ=255)
         ggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTg                                                                                        <  1:131084/101‑1 (MQ=255)
            gAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgcg                                                                                     >  2:1344807/1‑101 (MQ=255)
            gAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgcg                                                                                     <  1:3168702/101‑1 (MQ=255)
             aGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgcgc                                                                                    >  1:3684601/1‑101 (MQ=255)
              ggTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCt                                                                                   <  2:281103/101‑1 (MQ=255)
               gTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTg                                                                                  >  2:2150567/1‑101 (MQ=255)
               gTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTg                                                                                  >  2:4426998/1‑101 (MQ=255)
               gTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTg                                                                                  >  2:3008709/1‑101 (MQ=255)
                   tGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGACTTCCAGCGCCTCATCGGCCTGGCGCCGGCAt                                                                              >  1:2885627/1‑101 (MQ=255)
                   tGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCAt                                                                              <  1:4054697/101‑1 (MQ=255)
                   tGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCAt                                                                              <  2:3155581/101‑1 (MQ=255)
                     tAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTc                                                                            <  2:405479/101‑1 (MQ=255)
                        tCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTACAGCGCCTCATCGGCCTGGCGCTGGCATTCGcc                                                                         >  1:3802387/1‑101 (MQ=255)
                         cGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGccc                                                                        >  2:1648391/1‑101 (MQ=255)
                         cGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGccc                                                                        <  2:2631135/101‑1 (MQ=255)
                         cGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCAATCGccc                                                                        >  1:733740/1‑101 (MQ=255)
                          gATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCg                                                                       >  1:3414733/1‑101 (MQ=255)
                               gCTTGTGGCACGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTcgcg                                                                  <  1:2102695/101‑1 (MQ=255)
                                 ttGTGGCCCGCATAGCGCAGCACCTCGCGCACCGTCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGAt                                                                <  1:987784/101‑1 (MQ=255)
                                 ttGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGAt                                                                >  1:3913317/1‑101 (MQ=255)
                                 ttGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGAt                                                                <  2:778627/101‑1 (MQ=255)
                                  tgtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATc                                                               <  1:1721444/101‑1 (MQ=255)
                                  tgtcggccGCATACCGCCGCCGCTCACAGACCGGCTTGTCGCCAGCATTGATGAATTCCAGCGCCTCAACGGGCTGGCGCTGGCATTCGCCCGTCGCGATc                                                               <  1:219022/95‑1 (MQ=255)
                                   gtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCg                                                              <  1:525785/101‑1 (MQ=255)
                                     ggCCCGCATAGCGCAGCCCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGcc                                                            <  2:1793765/101‑1 (MQ=255)
                                     ggCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGcc                                                            >  1:307372/1‑101 (MQ=255)
                                      gCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCa                                                           >  1:2676882/1‑101 (MQ=255)
                                       cccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCGTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCAt                                                          <  1:2438531/101‑1 (MQ=255)
                                       cccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTCGCGCTGGCATTCGCCCGTcgccacccccct                                                          >  1:784642/1‑92 (MQ=255)
                                        ccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATg                                                         >  2:3885492/1‑101 (MQ=255)
                                        ccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATg                                                         <  2:1382560/101‑1 (MQ=255)
                                         cGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGa                                                        <  1:1050912/101‑1 (MQ=255)
                                         cGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGa                                                        <  1:1380422/101‑1 (MQ=255)
                                          gCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGGTGGCATTCGCCCGTCGCGATCGCCATgca                                                       >  2:3538508/1‑99 (MQ=255)
                                          gCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGaa                                                       >  1:2759297/1‑101 (MQ=255)
                                          gCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGaa                                                       >  2:482988/1‑101 (MQ=255)
                                            aTAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAca                                                     <  2:2319853/101‑1 (MQ=255)
                                              aGCGCAGCCCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAcagc                                                   <  1:3029343/101‑1 (MQ=255)
                                                  cagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGcc                                               <  2:2101673/101‑1 (MQ=255)
                                                   agcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCg                                              <  2:574946/101‑1 (MQ=255)
                                                      cccTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCtt                                           <  1:3380498/100‑1 (MQ=255)
                                                       ccTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTg                                          <  2:2737898/101‑1 (MQ=255)
                                                       ccTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTg                                          <  2:2033313/101‑1 (MQ=255)
                                                        cTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGt                                         >  1:3542708/1‑101 (MQ=255)
                                                         ccgcgCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGCCCTGACGCTGGCATTCGCCCGTCGCGATCGCCATTAACAGCAGCCTCTTGta                                        <  2:729173/100‑2 (MQ=255)
                                                          cgcgCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTcc                                       <  1:1976238/101‑1 (MQ=255)
                                                          cgcgCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTcc                                       <  2:4141057/101‑1 (MQ=255)
                                                           gcgcACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCg                                      >  1:3785032/1‑101 (MQ=255)
                                                            cgcACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCgg                                     <  1:2261144/101‑1 (MQ=255)
                                                             gcACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCggg                                    <  2:3129425/101‑1 (MQ=255)
                                                              cACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGa                                   >  2:2430788/1‑101 (MQ=255)
                                                                ccGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGaaa                                 <  2:3684348/101‑1 (MQ=255)
                                                                 cGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAAt                                <  2:1864525/101‑1 (MQ=255)
                                                                  ggCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATa                               >  2:3486547/1‑101 (MQ=255)
                                                                  ggCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATa                               >  1:601813/1‑101 (MQ=255)
                                                                  ggCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATa                               >  2:2558847/1‑101 (MQ=255)
                                                                   gCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAg                              <  1:1260466/101‑1 (MQ=255)
                                                                   gCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCCCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAg                              <  2:3988351/101‑1 (MQ=255)
                                                                     cTGTCGGCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCt                            <  1:1912049/101‑1 (MQ=255)
                                                                      tGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTg                           >  2:2387775/1‑101 (MQ=255)
                                                                      tGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTg                           >  1:75808/1‑101 (MQ=255)
                                                                       gTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGt                          <  1:4242430/101‑1 (MQ=255)
                                                                       gTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGt                          <  1:4010334/101‑1 (MQ=255)
                                                                       gTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGt                          <  2:3428313/101‑1 (MQ=255)
                                                                        tCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTa                         <  1:1003574/101‑1 (MQ=255)
                                                                         cGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTag                        >  1:3608757/1‑101 (MQ=255)
                                                                         cGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTag                        <  1:3529115/101‑1 (MQ=255)
                                                                          gCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTaga                       <  1:1139098/101‑1 (MQ=255)
                                                                          gCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTaga                       <  2:435326/101‑1 (MQ=255)
                                                                            ccGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagagc                     <  2:3272795/101‑2 (MQ=255)
                                                                            ccGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagagc                     <  2:4196165/101‑2 (MQ=255)
                                                                             cGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagagcg                    >  2:1627887/1‑99 (MQ=255)
                                                                               cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                  >  1:2287436/1‑101 (MQ=255)
                                                                               cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                  <  1:239052/101‑1 (MQ=255)
                                                                                aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCg                 >  1:1961253/1‑101 (MQ=255)
                                                                                aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCg                 >  2:3559924/1‑101 (MQ=255)
                                                                                   gatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCt              >  1:3089780/1‑101 (MQ=255)
                                                                                     tgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTg            <  1:2588/101‑1 (MQ=255)
                                                                                     tgaATGCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTg            <  2:1938488/101‑1 (MQ=255)
                                                                                      gaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTgg           <  2:2867781/101‑1 (MQ=255)
                                                                                       aaTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGa          <  2:4059065/101‑1 (MQ=255)
                                                                                        aTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGac         <  1:1636658/101‑1 (MQ=255)
                                                                                          tCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGacac       <  1:2343409/101‑1 (MQ=255)
                                                                                            cAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccc     >  2:1289569/1‑101 (MQ=255)
                                                                                             aGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAcccc    <  2:901444/101‑1 (MQ=255)
                                                                                              gcgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccccc   <  2:3060182/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCG  >  NC_014034/2959097‑2959292

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG