Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,959,273 T→C 100% T48T (ACA→ACG RCAP_rcc02771 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,959,2730T→C100.0% 734.7 199T48T (ACA→ACGRCAP_rcc02771TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (89/110):  ref base (0/0):  total (89/110)

Only 100 of 205 total aligned reads displayed.

CATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCT  >  NC_014034/2959176‑2959370
                                                                                                 |                                                                                                 
cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                                                                                                >  1:2287436/1‑101 (MQ=255)
cATCGATGAATTCCAGGGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                                                                                                <  2:1083326/101‑1 (MQ=255)
   tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc                                                                                             >  1:626731/1‑101 (MQ=255)
     atgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCtt                                                                                           <  1:3388074/101‑1 (MQ=255)
       gaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGGCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTgg                                                                                         >  2:770687/1‑101 (MQ=255)
        aaTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGa                                                                                        >  2:586430/1‑101 (MQ=255)
         aTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGac                                                                                       <  2:917693/101‑1 (MQ=255)
         aTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGac                                                                                       <  1:1636658/101‑1 (MQ=255)
         aTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGac                                                                                       <  1:1227626/101‑1 (MQ=255)
         aTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGac                                                                                       >  1:1099068/1‑101 (MQ=255)
             cAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccc                                                                                   >  2:1289569/1‑101 (MQ=255)
              aGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAcccc                                                                                  >  2:2644819/1‑101 (MQ=255)
               gcgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccccc                                                                                 <  1:129777/101‑1 (MQ=255)
               gcgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccccc                                                                                 <  2:3060182/101‑1 (MQ=255)
                 gcctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGc                                                                               <  2:2756104/98‑1 (MQ=255)
                 gcctCACCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGc                                                                               <  2:3228365/98‑1 (MQ=255)
                  cctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGcc                                                                              <  2:3119447/99‑1 (MQ=255)
                  cctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGcc                                                                              <  1:2912345/99‑1 (MQ=255)
                  cctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCCcc                                                                              <  1:2559010/99‑1 (MQ=255)
                   ctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCt                                                                             >  2:2094631/2‑101 (MQ=255)
                    tCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            >  1:1902413/1‑101 (MQ=255)
                    tCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            <  1:2460968/101‑1 (MQ=255)
                     cATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcg                                                                           >  2:2861641/1‑101 (MQ=255)
                      aTCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgc                                                                          >  1:2441365/1‑101 (MQ=255)
                      aTCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgc                                                                          >  1:1543825/1‑101 (MQ=255)
                        cGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgcgc                                                                        >  2:2275083/1‑101 (MQ=255)
                         ggCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgcgcg                                                                       >  2:1488795/1‑101 (MQ=255)
                           ccTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgcgcgcg                                                                     >  1:1504318/1‑101 (MQ=255)
                           ccTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgcgcgcg                                                                     <  1:3004948/101‑1 (MQ=255)
                           ccTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgcgcgcg                                                                     <  2:1355694/101‑1 (MQ=255)
                             tGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGAt                                                                   >  1:221171/1‑101 (MQ=255)
                             tGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGAt                                                                   <  1:1608798/101‑1 (MQ=255)
                                 gcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCa                                                               <  2:2217239/101‑1 (MQ=255)
                                 gcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCa                                                               <  1:102223/101‑1 (MQ=255)
                                 gcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCa                                                               >  1:2399973/1‑101 (MQ=255)
                                  cTGTCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCAt                                                              <  2:3672919/101‑1 (MQ=255)
                                   tGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATc                                                             >  2:1223211/1‑101 (MQ=255)
                                   tGGCATTCCCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGTCATc                                                             <  1:3538756/101‑1 (MQ=255)
                                    ggCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCg                                                            <  2:3961686/101‑1 (MQ=255)
                                     gCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGa                                                           >  1:2821985/1‑101 (MQ=255)
                                     gCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGa                                                           <  1:4028272/101‑1 (MQ=255)
                                     ccATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGa                                                           <  2:3087017/100‑1 (MQ=255)
                                      cATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAc                                                          <  1:2225458/101‑1 (MQ=255)
                                      cATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAc                                                          <  1:3911277/101‑1 (MQ=255)
                                      cATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAc                                                          <  1:3059956/101‑1 (MQ=255)
                                       aTTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAcc                                                         >  1:451869/1‑101 (MQ=255)
                                         tCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGa                                                       >  1:2087032/1‑101 (MQ=255)
                                         tCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGa                                                       <  1:2972231/101‑1 (MQ=255)
                                           gCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGacg                                                     >  2:1462254/1‑99 (MQ=255)
                                            cccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgc                                                    <  2:3414496/101‑1 (MQ=255)
                                             ccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcg                                                   <  1:754341/101‑1 (MQ=255)
                                               gTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGcc                                                 <  1:878529/101‑1 (MQ=255)
                                                 ggcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCt                                               >  2:1390836/2‑101 (MQ=255)
                                                 cgcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCt                                               >  2:3844689/1‑101 (MQ=255)
                                                  gcgATCGCCATGAACGGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACGCCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTc                                              <  2:355263/101‑1 (MQ=255)
                                                  gcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTc                                              >  1:4281035/1‑101 (MQ=255)
                                                  gcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTc                                              <  1:3720745/101‑1 (MQ=255)
                                                    gATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGa                                            >  2:4083117/1‑101 (MQ=255)
                                                     aTCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGaa                                           >  2:3062492/1‑101 (MQ=255)
                                                       cGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGGGCGCGATGTCATCGACCGAAGCGCCCTCGAAcc                                         >  1:3788657/1‑101 (MQ=255)
                                                        gCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAccc                                        <  1:999613/101‑1 (MQ=255)
                                                        gCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAccc                                        >  1:2091966/1‑101 (MQ=255)
                                                        gCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAccc                                        <  2:4280766/101‑1 (MQ=255)
                                                          cATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCgc                                      >  2:314599/1‑100 (MQ=255)
                                                           aTGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTc                                     <  1:2005204/101‑1 (MQ=255)
                                                            tGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcg                                    >  1:2382067/1‑101 (MQ=255)
                                                            tGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcg                                    >  2:607872/1‑101 (MQ=255)
                                                             gAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgc                                   <  1:3145089/101‑1 (MQ=255)
                                                             gAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgc                                   <  2:2966011/101‑1 (MQ=255)
                                                               aCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCgcgc                                 >  1:600695/1‑101 (MQ=255)
                                                               aCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCgcgc                                 >  1:434208/1‑101 (MQ=255)
                                                                cagcagCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCa                                <  1:2734039/101‑1 (MQ=255)
                                                                 agcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAg                               <  2:2154733/101‑1 (MQ=255)
                                                                  gcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGa                              <  2:3326292/101‑1 (MQ=255)
                                                                  gcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGa                              <  2:2399816/101‑1 (MQ=255)
                                                                    agcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaaa                            <  1:2327041/101‑1 (MQ=255)
                                                                      ccGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAaca                          <  1:4173689/101‑1 (MQ=255)
                                                                          ttGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACttt                      >  2:2687553/1‑101 (MQ=255)
                                                                          ttGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACttt                      <  2:1504213/101‑1 (MQ=255)
                                                                          ttGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACttt                      >  2:201669/1‑101 (MQ=255)
                                                                             tCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcg                   <  2:3802134/101‑1 (MQ=255)
                                                                             tCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcg                   <  2:3564403/101‑1 (MQ=255)
                                                                              ccGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcgc                  >  2:1540925/1‑101 (MQ=255)
                                                                               ctggAAATAGCTGTAGAGTGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGCCATCGACCGAAGCGCCCTCGAACCCGCCGCGCAGAAACACTTTCcgcg                 <  2:702259/99‑1 (MQ=255)
                                                                                  gAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCgcgcgc              <  2:3875606/101‑1 (MQ=255)
                                                                                   aaaTAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGcc             >  1:3241099/1‑101 (MQ=255)
                                                                                    aagagatGTAGAGGGACGCCTTGGGCCCCCCCGCCTCGCGCGCGGTGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCg            <  2:4070303/95‑1 (MQ=255)
                                                                                      tAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTc          <  2:3542460/101‑1 (MQ=255)
                                                                                      tAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTc          <  1:1055641/101‑1 (MQ=255)
                                                                                      tAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTc          >  2:2348649/1‑101 (MQ=255)
                                                                                       aGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCa         >  1:2591636/1‑101 (MQ=255)
                                                                                       aGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCa         >  1:2039948/1‑101 (MQ=255)
                                                                                       aGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCa         >  2:793892/1‑101 (MQ=255)
                                                                                         cTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAg       >  1:1199396/1‑101 (MQ=255)
                                                                                             agagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGGGCAGAAACCCTTTCCGCGCGCCGTCGAGGAcc   >  2:288001/1‑101 (MQ=255)
                                                                                             agagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGAcc   <  2:350364/101‑1 (MQ=255)
                                                                                             agagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGAcc   >  1:3410091/1‑101 (MQ=255)
                                                                                             agagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGAGCCCTCGAACCCGTCGAGCAGAAACACTTTCCGCGCGCCGTCAAGAAcc   >  1:901792/1‑101 (MQ=255)
                                                                                              gagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCt  >  2:1727656/1‑101 (MQ=255)
                                                                                              gagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCt  <  2:2086895/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
CATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCT  >  NC_014034/2959176‑2959370

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG