Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,499 A→G 100% E123E (GAA→GAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,4990A→G100.0% 873.0 229E123E (GAA→GAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (125/104):  ref base (0/0):  total (125/104)

Only 100 of 235 total aligned reads displayed.

GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596
                                                                                                  |                                                                                                 
gACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgc                                                                                                 >  2:3256327/1‑101 (MQ=255)
gACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgc                                                                                                 >  2:1709052/1‑101 (MQ=255)
gACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgc                                                                                                 <  1:1257581/101‑1 (MQ=255)
 aCCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgcg                                                                                                >  2:3599079/1‑101 (MQ=255)
   cGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGcc                                                                                              <  2:437130/101‑1 (MQ=255)
   cGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGcc                                                                                              <  2:2827454/101‑1 (MQ=255)
    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             <  1:3354482/101‑1 (MQ=255)
     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgg                                                                                            >  1:4054974/1‑101 (MQ=255)
     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgg                                                                                            <  1:3381617/101‑1 (MQ=255)
      gcgcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGt                                                                                           <  2:15631/101‑1 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         >  2:2258953/1‑101 (MQ=255)
         cAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAc                                                                                        <  2:4192797/101‑1 (MQ=255)
          aGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCAAGAGGTTCACCCTCTGGAGCCCCCTGACACCCCTGCAAAAGGCCATGACGGGGCCGGTGACg                                                                                       >  2:2099623/1‑101 (MQ=255)
            gTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGt                                                                                     >  1:261522/1‑101 (MQ=255)
               aCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCt                                                                                  <  1:2667313/101‑1 (MQ=255)
               aCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCt                                                                                  >  2:2280241/1‑101 (MQ=255)
               aCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCt                                                                                  >  1:1429379/1‑101 (MQ=255)
                  cgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTggg                                                                               <  2:810750/101‑1 (MQ=255)
                     tttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCg                                                                            <  1:967235/101‑1 (MQ=255)
                     tttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCg                                                                            >  2:4039723/1‑101 (MQ=255)
                       tCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCa                                                                          <  2:1355441/101‑1 (MQ=255)
                       tCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCa                                                                          <  1:1205280/101‑1 (MQ=255)
                        cGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCaa                                                                         >  2:1797128/1‑101 (MQ=255)
                         gCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCaaa                                                                        >  1:1099315/1‑101 (MQ=255)
                           cccAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAcc                                                                      <  2:2677059/100‑1 (MQ=255)
                           aCCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAcc                                                                      >  2:4083009/1‑101 (MQ=255)
                            ccAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCg                                                                     <  2:2531485/101‑1 (MQ=255)
                               aCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGcc                                                                  >  1:2382687/1‑101 (MQ=255)
                                ccTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCg                                                                 >  1:2948820/1‑101 (MQ=255)
                                   gTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGagg                                                              >  2:3248749/1‑99 (MQ=255)
                                    tCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGt                                                             <  1:3211364/101‑1 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  1:2713417/101‑1 (MQ=255)
                                      gATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                           >  2:774397/1‑101 (MQ=255)
                                      gATGATGGTTCAGCAGTTCAACCTCTGGACCCACTTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                           <  2:4389815/101‑1 (MQ=255)
                                       aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                          <  1:168959/101‑1 (MQ=255)
                                         ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAc                                                        <  1:3256559/101‑1 (MQ=255)
                                         ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAc                                                        >  1:2887551/1‑101 (MQ=255)
                                          gttttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       <  1:3530283/98‑1 (MQ=255)
                                          gttttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       <  1:1849152/98‑1 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       <  1:1867538/101‑1 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       <  1:2053341/101‑1 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       >  2:1432152/1‑101 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       >  1:2248856/1‑101 (MQ=255)
                                            gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     >  2:451088/1‑101 (MQ=255)
                                            gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     >  2:1284910/1‑101 (MQ=255)
                                             ttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCt                                                    <  2:3516504/101‑1 (MQ=255)
                                              tCCAGCAGTTCAACCTCTGGATCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAGACCGGCCGAGGTCGAACCGCTc                                                   <  1:42808/101‑1 (MQ=255)
                                              tCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTc                                                   >  1:1059885/1‑101 (MQ=255)
                                                 agcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcc                                                >  2:4071280/1‑101 (MQ=255)
                                                  gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                               <  1:3097581/101‑1 (MQ=255)
                                                  gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                               <  1:1950997/101‑1 (MQ=255)
                                                  gcaaTTAAACCTCTGGAGCCGCATGACGATCCTGCAAAATGTCATGGAGGCGCGGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGtcc                                               <  1:2552270/101‑1 (MQ=255)
                                                    agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                             <  2:422941/101‑1 (MQ=255)
                                                     gTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCa                                            >  1:786933/1‑101 (MQ=255)
                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         >  2:147219/1‑101 (MQ=255)
                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         >  1:621151/1‑101 (MQ=255)
                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         >  2:3179711/1‑101 (MQ=255)
                                                          cccgcgggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                       <  1:2319842/95‑1 (MQ=255)
                                                           ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                      >  2:3776879/1‑101 (MQ=255)
                                                             tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                    <  1:2024737/101‑1 (MQ=255)
                                                             tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                    >  2:2415992/1‑101 (MQ=255)
                                                              ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                   >  1:1765598/1‑101 (MQ=255)
                                                               tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                  >  2:3662556/1‑101 (MQ=255)
                                                               tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                  >  2:3852665/1‑101 (MQ=255)
                                                               tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                  >  1:1022751/1‑101 (MQ=255)
                                                                ggAGCCACATGAGGACCCTGCAAAATGTCATGGAGCCCCCGGTGACGGTTCTGGGCCCCAAACCGGCCGAGGCCGAACCGCTGGCCCGCAGCCTTCTGGca                                 >  2:17030/1‑100 (MQ=255)
                                                                ggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGcc                                 >  1:128620/1‑101 (MQ=255)
                                                                 gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                <  1:2431861/101‑1 (MQ=255)
                                                                  aGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCaa                               >  1:3005439/1‑101 (MQ=255)
                                                                    ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                             <  2:1895895/101‑1 (MQ=255)
                                                                      acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTc                           >  1:4257353/1‑101 (MQ=255)
                                                                      acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTc                           >  1:3333276/1‑101 (MQ=255)
                                                                      acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTc                           >  1:470153/1‑101 (MQ=255)
                                                                       caTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCg                          <  1:3901359/101‑1 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         >  2:2962423/1‑101 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         >  2:255303/1‑101 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         >  2:151512/1‑101 (MQ=255)
                                                                        aTGACGATCCTGCAAAAGGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         <  1:2592703/101‑1 (MQ=255)
                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       <  2:4138041/101‑1 (MQ=255)
                                                                            cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     <  2:2016998/101‑1 (MQ=255)
                                                                             gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                    <  2:2887343/101‑1 (MQ=255)
                                                                             gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                    <  2:557973/101‑1 (MQ=255)
                                                                             gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                    >  1:3723261/1‑101 (MQ=255)
                                                                              aTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCgg                   >  2:3481326/1‑101 (MQ=255)
                                                                                 cTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGa                >  2:4325521/1‑101 (MQ=255)
                                                                                   gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa              >  1:723071/1‑101 (MQ=255)
                                                                                   gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa              >  2:3024974/1‑101 (MQ=255)
                                                                                    cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa             >  2:672184/1‑101 (MQ=255)
                                                                                        aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc         <  2:1059797/101‑1 (MQ=255)
                                                                                           tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc      <  1:4071540/101‑1 (MQ=255)
                                                                                           tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc      <  2:2248711/101‑1 (MQ=255)
                                                                                           tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc      <  2:3074489/101‑1 (MQ=255)
                                                                                            cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg     <  1:439138/101‑1 (MQ=255)
                                                                                            cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg     <  1:3612587/101‑1 (MQ=255)
                                                                                             aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc    >  1:436855/1‑101 (MQ=255)
                                                                                             aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc    >  2:118457/1‑101 (MQ=255)
                                                                                              tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc   <  2:2192104/101‑1 (MQ=255)
                                                                                              tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc   <  2:3883700/101‑1 (MQ=255)
                                                                                              tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc   >  1:674966/1‑101 (MQ=255)
                                                                                               ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt  <  2:3558699/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG