Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,535 A→G 100% E135E (GAA→GAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5350A→G100.0% 924.2 246E135E (GAA→GAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (122/124):  ref base (0/0):  total (122/124)

Only 100 of 255 total aligned reads displayed.

TCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCG  >  NC_014034/2960437‑2960632
                                                                                                  |                                                                                                 
tCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGt                                                                                                 <  1:996519/101‑1 (MQ=255)
 cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                <  1:2713417/101‑1 (MQ=255)
 cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                >  2:2934833/1‑101 (MQ=255)
 cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                >  2:429021/1‑101 (MQ=255)
  gATGATGGTTCAGCAGTTCAACCTCTGGACCCACTTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                                                               <  2:4389815/101‑1 (MQ=255)
   aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCCCATGACGATCCTGCAAAATGTCATGGAGCCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                                                              >  1:3990134/1‑101 (MQ=39)
     ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAc                                                                                            >  1:2887551/1‑101 (MQ=255)
     ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAc                                                                                            <  1:3256559/101‑1 (MQ=255)
      gttttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                                                           <  1:3530283/98‑1 (MQ=255)
      gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                                                           >  1:2657425/1‑101 (MQ=255)
        gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                                                         <  1:335211/101‑1 (MQ=255)
        gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                                                         >  2:451088/1‑101 (MQ=255)
         ttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCt                                                                                        <  2:3516504/101‑1 (MQ=255)
           ccAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCg                                                                                      <  2:757107/101‑1 (MQ=255)
             agcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcc                                                                                    >  2:4071280/1‑101 (MQ=255)
             agcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcc                                                                                    >  1:3085842/1‑101 (MQ=255)
              gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   <  1:1950997/101‑1 (MQ=255)
              gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   <  1:3097581/101‑1 (MQ=255)
              gcaaTTAAACCTCTGGAGCCGCATGACGATCCTGCAAAATGTCATGGAGGCGCGGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGtcc                                                                                   <  1:2552270/101‑1 (MQ=255)
               cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                                                                  >  1:4271773/1‑101 (MQ=255)
                agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                                                                 <  2:422941/101‑1 (MQ=255)
                 gTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCa                                                                                >  1:632604/1‑101 (MQ=255)
                  ttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAg                                                                               <  2:2850674/101‑1 (MQ=255)
                    cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                                                             >  2:3179711/1‑101 (MQ=255)
                    cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                                                             >  2:147219/1‑101 (MQ=255)
                      aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                                                           <  1:1997977/101‑1 (MQ=255)
                       ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                                                          >  2:3776879/1‑101 (MQ=255)
                         tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                                                        <  1:2024737/101‑1 (MQ=255)
                          ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                                                       <  2:1402835/101‑1 (MQ=255)
                          ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                                                       >  1:1765598/1‑101 (MQ=255)
                           tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                                                      >  1:1022751/1‑101 (MQ=255)
                           tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                                                      >  2:3852665/1‑101 (MQ=255)
                                 cacaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGt                                                                <  2:3252975/101‑1 (MQ=255)
                                  acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTc                                                               >  1:470153/1‑101 (MQ=255)
                                   caTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCg                                                              <  1:3901359/101‑1 (MQ=255)
                                    aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                                                             <  1:508697/101‑1 (MQ=255)
                                    aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                                                             >  2:2962423/1‑101 (MQ=255)
                                     tGACGATCCTGCAAAATGTCATGGAGGCGCCGGTTACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGc                                                            <  2:685846/101‑1 (MQ=255)
                                       aCGATCCTGCGAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                                                          >  2:1600705/1‑101 (MQ=255)
                                        cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                                                         <  2:2016998/101‑1 (MQ=255)
                                         gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                                                        <  2:557973/101‑1 (MQ=255)
                                          aTCCTGAAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCgg                                                       <  2:1540139/101‑1 (MQ=255)
                                           tCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGc                                                      >  2:2369467/1‑101 (MQ=255)
                                            ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                                                     >  1:1066932/1‑101 (MQ=255)
                                               gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  <  1:3434797/101‑1 (MQ=255)
                                               gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  >  2:3024974/1‑101 (MQ=255)
                                               gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  >  1:723071/1‑101 (MQ=255)
                                                 aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag                                                <  2:1264681/101‑2 (MQ=255)
                                                 aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag                                                >  1:3669701/1‑100 (MQ=255)
                                                       tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                          <  2:3074489/101‑1 (MQ=255)
                                                        cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                         <  1:439138/101‑1 (MQ=255)
                                                        cATGGAGACGCCGGTGACGGGTCTGGGCAGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCCGACCAAGGTCTGCATAGGCGCCAAGGCCGACg                                         >  2:2431193/1‑101 (MQ=255)
                                                         aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                        >  2:170348/1‑101 (MQ=255)
                                                         aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                        >  2:118457/1‑101 (MQ=255)
                                                         aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                        >  1:436855/1‑101 (MQ=255)
                                                          tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcg                                       >  2:225877/1‑100 (MQ=255)
                                                          tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGGCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGCCGCCATCGGCGACAAGGCCGACGcc                                       >  2:2099297/1‑101 (MQ=255)
                                                           ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                      <  2:3558699/101‑1 (MQ=255)
                                                            gaggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTg                                     <  1:17043/98‑1 (MQ=255)
                                                            gaggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTg                                     >  1:3579083/4‑101 (MQ=255)
                                                              ggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGc                                   <  1:3777132/100‑1 (MQ=255)
                                                              ggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGc                                   >  2:2988192/2‑101 (MQ=255)
                                                                   cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgcg                              >  2:1457502/1‑101 (MQ=255)
                                                                   cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGACTGGCCcgcg                              >  1:4325426/1‑101 (MQ=255)
                                                                    ggTGACGGTTCTGGGCCGCAAACTGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                             >  2:1762866/1‑101 (MQ=255)
                                                                    ggTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                             >  2:927485/1‑101 (MQ=255)
                                                                    ggTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                             >  1:3283688/1‑101 (MQ=255)
                                                                       gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                          <  1:774477/101‑1 (MQ=255)
                                                                       gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                          >  2:1861365/1‑101 (MQ=255)
                                                                        gcGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                         <  2:480249/100‑1 (MQ=255)
                                                                        aCGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                         >  1:4204063/1‑101 (MQ=255)
                                                                         cGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctc                        <  1:255330/101‑1 (MQ=255)
                                                                          ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                       >  2:1307777/1‑101 (MQ=255)
                                                                          ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                       <  2:2184122/101‑1 (MQ=255)
                                                                           gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                      <  1:1425747/101‑1 (MQ=255)
                                                                            ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                     <  1:2246234/101‑1 (MQ=255)
                                                                             tCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCgg                    <  1:1314892/101‑1 (MQ=255)
                                                                              cTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCggg                   >  1:675089/1‑101 (MQ=255)
                                                                               tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                  >  2:219309/1‑101 (MQ=255)
                                                                                  gCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcggc               <  2:333965/101‑1 (MQ=255)
                                                                                   ccGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGcc              <  2:1066844/101‑1 (MQ=255)
                                                                                   ccGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGcc              <  2:1896345/101‑1 (MQ=255)
                                                                                    cGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCca             <  2:1429272/101‑1 (MQ=255)
                                                                                     gCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcag            <  1:4103124/101‑1 (MQ=255)
                                                                                      cAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc           <  1:125794/101‑1 (MQ=255)
                                                                                      aaacccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc           <  2:1968649/97‑1 (MQ=255)
                                                                                       aaaCCGGTCGATGTCGAACCGCTCGCCCGCAGCCTTCCGGCCAAGGTCGGCATCGGCGGCAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  2:3989878/101‑1 (MQ=255)
                                                                                       aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  2:2608043/101‑1 (MQ=255)
                                                                                       aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  1:2280386/101‑1 (MQ=255)
                                                                                       aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  2:3620553/101‑1 (MQ=255)
                                                                                       aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  1:2416149/101‑1 (MQ=255)
                                                                                       aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  1:4077013/101‑1 (MQ=255)
                                                                                        aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag         >  2:3110904/1‑101 (MQ=255)
                                                                                         aCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCagcagc        <  1:3852918/101‑1 (MQ=255)
                                                                                          ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca       <  1:1797247/101‑1 (MQ=255)
                                                                                          ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca       >  2:2776205/1‑101 (MQ=255)
                                                                                            ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc     <  1:2099760/101‑1 (MQ=255)
                                                                                             gCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcg    <  2:3723008/101‑1 (MQ=255)
                                                                                              ccGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgc   <  1:1600811/101‑1 (MQ=255)
                                                                                               cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg  <  1:118478/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
TCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCG  >  NC_014034/2960437‑2960632

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG