Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,586 A→G 100% K152K (AAA→AAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5860A→G100.0% 925.1 246K152K (AAA→AAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (122/124):  ref base (0/0):  total (122/124)

Only 100 of 253 total aligned reads displayed.

AATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960488‑2960683
                                                                                                  |                                                                                                 
 aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc                                                                                                <  2:1059797/101‑1 (MQ=255)
 aTGTCATGGAGGCGCCGGTGACGGTGCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc                                                                                                <  2:1737713/101‑1 (MQ=255)
    tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                                                                             <  1:4071540/101‑1 (MQ=255)
    tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                                                                             >  1:2604631/1‑101 (MQ=255)
    tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                                                                             <  2:3074489/101‑1 (MQ=255)
     cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                                                                            <  1:3612587/101‑1 (MQ=255)
     cATGGAGACGCCGGTGACGGGTCTGGGCAGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCCGACCAAGGTCTGCATAGGCGCCAAGGCCGACg                                                                                            >  2:2431193/1‑101 (MQ=255)
      aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  2:170348/1‑101 (MQ=255)
      aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  1:436855/1‑101 (MQ=255)
       tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcg                                                                                          >  2:225877/1‑100 (MQ=255)
       tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                          >  1:674966/1‑101 (MQ=255)
       tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGGCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGCCGCCATCGGCGACAAGGCCGACGcc                                                                                          >  2:2099297/1‑101 (MQ=255)
        ggAGGCGCCGTTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                                                                         <  2:1935161/101‑1 (MQ=255)
         gaggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTg                                                                                        >  1:3579083/4‑101 (MQ=255)
           ggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGc                                                                                      <  1:3777132/100‑1 (MQ=255)
           ggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGc                                                                                      <  2:3113921/100‑1 (MQ=255)
             cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                    >  1:58264/1‑101 (MQ=255)
             cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                    <  1:200378/101‑1 (MQ=255)
             cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                    >  2:146399/1‑101 (MQ=255)
              gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                                                                   >  2:1988876/1‑101 (MQ=255)
              gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                                                                   <  2:3005218/101‑1 (MQ=255)
              gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                                                                   <  2:1224478/101‑1 (MQ=255)
                cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGACTGGCCcgcg                                                                                 >  1:4325426/1‑101 (MQ=255)
                 ggTGACGGTTCTGGGCCGCAAACTGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                                                                                >  2:1762866/1‑101 (MQ=255)
                 ggTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                                                                                >  1:3283688/1‑101 (MQ=255)
                 ggTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                                                                                >  2:3780848/1‑101 (MQ=255)
                    gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             <  1:774477/101‑1 (MQ=255)
                    gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             >  2:1861365/1‑101 (MQ=255)
                     gcGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                                                                            <  2:480249/100‑1 (MQ=255)
                       ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                                                                          <  2:2184122/101‑1 (MQ=255)
                        gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                                                                         <  2:2948599/101‑1 (MQ=255)
                        gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                                                                         <  2:742933/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                        <  2:1581205/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                        <  1:2246234/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                        >  1:942402/1‑101 (MQ=255)
                            tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  2:219309/1‑101 (MQ=255)
                             gggCCGCAAAACGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACCAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcg                                                                    >  1:3855794/1‑101 (MQ=255)
                              ggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcgg                                                                   >  2:437050/1‑101 (MQ=255)
                               gCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcggc                                                                  <  2:333965/101‑1 (MQ=255)
                                   cAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc                                                              <  1:125794/101‑1 (MQ=255)
                                    aaaCCGGTCGATGTCGAACCGCTCGCCCGCAGCCTTCCGGCCAAGGTCGGCATCGGCGGCAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             <  2:3989878/101‑1 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             >  1:1340360/1‑101 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             <  1:1709163/101‑1 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             >  2:1088550/1‑101 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             >  1:3094873/1‑101 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             <  1:2416149/101‑1 (MQ=255)
                                     aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag                                                            >  1:3511881/1‑101 (MQ=255)
                                        cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa                                                         >  1:1431077/1‑101 (MQ=255)
                                        cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa                                                         >  2:2840109/1‑101 (MQ=255)
                                         ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        <  1:4083269/101‑1 (MQ=255)
                                         ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        <  1:2099760/101‑1 (MQ=255)
                                          gCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcg                                                       <  2:261495/101‑1 (MQ=255)
                                           ccGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgc                                                      <  1:1600811/101‑1 (MQ=255)
                                            cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg                                                     <  1:118478/101‑1 (MQ=255)
                                            cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg                                                     >  1:270392/1‑101 (MQ=255)
                                            cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg                                                     >  1:3987275/1‑101 (MQ=255)
                                             gaggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACgcgc                                                    >  2:2112505/4‑101 (MQ=255)
                                              aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgcc                                                   >  1:4152368/3‑101 (MQ=255)
                                              aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgcc                                                   <  1:1285003/99‑1 (MQ=255)
                                               ggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccg                                                  >  1:477232/2‑101 (MQ=255)
                                               ggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccg                                                  <  1:2130512/100‑1 (MQ=255)
                                                gTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccgc                                                 >  2:2783418/1‑101 (MQ=255)
                                                gTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccgc                                                 >  1:4398884/1‑101 (MQ=255)
                                                  cGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCa                                               <  1:1762985/101‑1 (MQ=255)
                                                  cGAACCGCTCGCACGGAGCCTTCTCGCGAAGGTCGGCCTCGGCGACAAGGCCGACTCCTGGCCGGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCa                                               <  2:4059193/101‑1 (MQ=37)
                                                         cTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcg                                        >  2:3571149/1‑101 (MQ=255)
                                                          tCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCtgc                                       >  2:3752744/1‑101 (MQ=255)
                                                          tCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcgc                                       <  1:456426/101‑1 (MQ=255)
                                                          tCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcgc                                       >  2:1319170/1‑101 (MQ=255)
                                                           cGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcgcg                                      >  2:3173825/1‑101 (MQ=255)
                                                               cGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGct                                  >  1:3456342/1‑101 (MQ=255)
                                                               cGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGct                                  <  2:454167/101‑1 (MQ=255)
                                                                gCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctc                                 >  1:705677/1‑101 (MQ=255)
                                                                 cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                                <  2:1173444/101‑1 (MQ=255)
                                                                   gCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgc                              >  1:2862095/1‑101 (MQ=255)
                                                                   gCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgc                              <  2:3669448/101‑1 (MQ=255)
                                                                     cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                            >  2:2730503/1‑101 (MQ=255)
                                                                     cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                            <  1:1886185/101‑1 (MQ=255)
                                                                      ttCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatg                           >  1:1636729/1‑101 (MQ=255)
                                                                         tGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAg                        >  2:2556709/1‑101 (MQ=255)
                                                                          ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       >  1:4226902/1‑101 (MQ=255)
                                                                          ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       <  1:3684942/101‑1 (MQ=255)
                                                                          ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       <  2:3481386/101‑1 (MQ=255)
                                                                           gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                      <  1:1457608/101‑1 (MQ=255)
                                                                             cAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    >  2:1445599/1‑101 (MQ=255)
                                                                             cAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    >  1:3241370/1‑101 (MQ=255)
                                                                             cAACGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    <  2:1396160/101‑1 (MQ=255)
                                                                              aaGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCa                   <  2:4257084/101‑1 (MQ=255)
                                                                               aGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAg                  <  2:4203802/101‑1 (MQ=255)
                                                                               aGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAg                  >  2:3262273/1‑101 (MQ=255)
                                                                                   cGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGgcgc              <  2:1553530/101‑1 (MQ=255)
                                                                                     gCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtt            <  2:902195/101‑1 (MQ=255)
                                                                                     gCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtt            <  2:3523716/101‑1 (MQ=255)
                                                                                       aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt          >  1:1085002/1‑101 (MQ=255)
                                                                                          ggCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGtt       >  2:3882026/1‑101 (MQ=255)
                                                                                          ggCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGtt       >  2:2691800/1‑101 (MQ=255)
                                                                                          ggCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGtt       <  2:719531/101‑1 (MQ=255)
                                                                                           gCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTc      <  2:489295/101‑1 (MQ=255)
                                                                                            cGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcg     <  1:2461965/101‑1 (MQ=255)
                                                                                               cAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgacg  >  1:1414196/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960488‑2960683

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG