Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,962,711 A→G 100% K21E (AAG→GAG)  hisM → polar amino acid ABC transporter permease HisM

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,962,7110A→G100.0% 816.7 216K21E (AAG→GAG) hisMpolar amino acid ABC transporter permease HisM
Reads supporting (aligned to +/- strand):  new base (94/122):  ref base (0/0):  total (94/122)

Only 100 of 225 total aligned reads displayed.

GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808
                                                                                                  |                                                                                                 
 cgacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgc                                                                                                <  1:1373198/101‑1 (MQ=255)
  gacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgcg                                                                                               >  2:601017/1‑101 (MQ=255)
  gacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgcg                                                                                               >  1:828271/1‑101 (MQ=255)
   acgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgcgc                                                                                              <  2:826698/101‑1 (MQ=255)
         gcgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgctg                                                                                        <  1:2231527/101‑1 (MQ=255)
            ggcgAAGCGCAACGGAAGGCCGCCGCATGAGCGACAGCGGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGGGCTGCcg                                                                                     <  2:1211771/101‑1 (MQ=255)
               gAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCgcgc                                                                                  >  1:3358690/1‑101 (MQ=255)
                aaGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCa                                                                                 <  1:3476450/101‑1 (MQ=255)
                 aGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCNNCGCAg                                                                                >  1:1924188/1‑101 (MQ=255)
                 aGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAg                                                                                <  1:3333687/101‑1 (MQ=255)
                    gcAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGa                                                                             <  1:2099062/101‑1 (MQ=255)
                      aaCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           >  2:1491930/1‑101 (MQ=255)
                       aCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACtt                                                                          <  2:167513/101‑1 (MQ=255)
                         ggAAGTCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  2:558423/101‑1 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGGAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  1:2719987/101‑1 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        >  2:2304924/1‑101 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  1:4417214/101‑1 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  1:4234101/101‑1 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       <  2:1708618/101‑1 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       <  2:1103431/101‑1 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       >  1:4016503/1‑101 (MQ=255)
                           aaGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACg                                                                      <  1:2947258/101‑1 (MQ=255)
                            aGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGc                                                                     <  2:1565880/101‑1 (MQ=255)
                                cgcgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTg                                                                 <  2:490220/101‑1 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               <  2:4333339/101‑1 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               >  2:2725437/1‑101 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               >  1:2122977/1‑101 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               >  2:2100520/1‑101 (MQ=255)
                                   ggCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTGGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCa                                                              >  1:3768234/1‑101 (MQ=255)
                                   ggCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCa                                                              <  2:963588/101‑1 (MQ=255)
                                   ggCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCa                                                              <  1:219394/101‑1 (MQ=255)
                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             >  1:1794845/1‑101 (MQ=255)
                                     cATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATg                                                            <  2:1498801/101‑1 (MQ=255)
                                      aTGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGa                                                           >  1:741850/1‑101 (MQ=255)
                                      aTGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGa                                                           <  1:552155/101‑1 (MQ=255)
                                         aGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGTTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGAAGTGACTTCACCCTCTGCCAGGAAtt                                                        >  2:1455741/1‑101 (MQ=255)
                                          gCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTc                                                       <  1:3651223/101‑1 (MQ=255)
                                           cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      <  1:201267/101‑1 (MQ=255)
                                             aCAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAcc                                                    >  1:11271/1‑101 (MQ=255)
                                             aCAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAcc                                                    <  2:2585470/101‑1 (MQ=255)
                                              cAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAccc                                                   <  2:2652559/101‑1 (MQ=255)
                                               aGCTGCCCGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCt                                                  <  1:2829388/101‑1 (MQ=255)
                                               aGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCt                                                  <  1:1492035/101‑1 (MQ=255)
                                               aGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCt                                                  >  1:2972629/1‑101 (MQ=255)
                                                gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 <  2:3134154/101‑1 (MQ=255)
                                                gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 <  1:443002/101‑1 (MQ=255)
                                                gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 <  2:3350815/101‑1 (MQ=255)
                                                  tGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGAt                                               <  1:2490807/101‑1 (MQ=255)
                                                  tGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGAt                                               <  1:1821662/101‑1 (MQ=255)
                                                  tGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGAt                                               <  2:3581321/101‑1 (MQ=255)
                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              >  1:3156401/1‑101 (MQ=255)
                                                    cACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             <  2:202214/101‑1 (MQ=255)
                                                     aCGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCgg                                            >  1:2347330/1‑101 (MQ=255)
                                                       gCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCt                                          >  2:1317023/1‑101 (MQ=255)
                                                        cAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCGGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTc                                         <  2:2986831/101‑1 (MQ=255)
                                                        cAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTc                                         >  2:3754657/1‑101 (MQ=255)
                                                            tcatcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgggg                                     >  2:250638/1‑101 (MQ=255)
                                                              atcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCt                                   <  2:4087273/101‑1 (MQ=255)
                                                              atcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCt                                   >  2:640887/1‑101 (MQ=255)
                                                              atcGCGGATTACCCCTTCCGCTCGCTGGGCCATGGCGAGCGCGTCCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGcc                                   >  2:3968981/1‑100 (MQ=255)
                                                               tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  >  2:3406319/1‑101 (MQ=255)
                                                                cgcgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGa                                 <  2:4145656/101‑1 (MQ=255)
                                                                  cgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATc                               >  1:1648012/1‑101 (MQ=255)
                                                                  cgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATc                               >  1:4263059/1‑101 (MQ=255)
                                                                   ggATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCt                              <  2:2882619/101‑1 (MQ=255)
                                                                    gATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCCGGGCTGATCTg                             >  2:2679510/1‑101 (MQ=255)
                                                                     aTTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTgg                            <  2:3060790/101‑1 (MQ=255)
                                                                      ttACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGa                           <  2:3594020/101‑1 (MQ=255)
                                                                        tcGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAc                         <  1:1017498/100‑1 (MQ=255)
                                                                         cGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTNGGGGCTGATCTGGANCg                        >  2:880935/1‑101 (MQ=255)
                                                                         cGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACg                        <  1:601085/101‑1 (MQ=255)
                                                                         cGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACg                        >  1:2443500/1‑101 (MQ=255)
                                                                          gCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGt                       >  1:3607397/1‑101 (MQ=255)
                                                                             ttCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTa                    <  1:1317120/101‑1 (MQ=255)
                                                                             ttCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTa                    <  1:4349646/101‑1 (MQ=255)
                                                                              tCCGCTCGCTGGGCTATTGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  2:4016247/101‑1 (MQ=255)
                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCTCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  1:724052/101‑1 (MQ=255)
                                                                               ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  2:3865107/101‑1 (MQ=255)
                                                                                cgctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTCttt                 >  2:2342047/1‑101 (MQ=255)
                                                                                cgctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAttt                 <  2:732689/101‑1 (MQ=255)
                                                                                cgctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAttt                 >  1:1587132/1‑101 (MQ=255)
                                                                                 gctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTc                <  1:1869364/101‑1 (MQ=255)
                                                                                  ctcgctGGGCTATGGCGNGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCg               <  2:1924059/101‑1 (MQ=255)
                                                                                   tcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCgg              >  2:1593966/1‑101 (MQ=255)
                                                                                     gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCTCCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCa            <  2:2273426/101‑1 (MQ=255)
                                                                                     gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGGACGTCTATTTTGGCa            >  1:3089319/1‑101 (MQ=255)
                                                                                     gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGcc            >  2:1404425/1‑100 (MQ=255)
                                                                                      ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  2:21978/101‑1 (MQ=255)
                                                                                      ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  1:1255387/101‑1 (MQ=255)
                                                                                      ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  2:4262790/101‑1 (MQ=255)
                                                                                       tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          >  1:1204455/1‑101 (MQ=255)
                                                                                       tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          <  1:1278699/101‑1 (MQ=255)
                                                                                       tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          >  2:280285/1‑101 (MQ=255)
                                                                                       tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          <  2:1849967/101‑1 (MQ=255)
                                                                                         ggCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCt        >  1:1516898/1‑101 (MQ=255)
                                                                                           cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTTGGCACGCTgg      >  1:302971/1‑101 (MQ=255)
                                                                                           cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTgg      >  1:2476176/1‑101 (MQ=255)
                                                                                           cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTgg      <  1:2901479/101‑1 (MQ=255)
                                                                                             aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgcg    <  1:1564113/101‑1 (MQ=255)
                                                                                               ggCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCt  <  1:3434657/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG