Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,036 A→C 10.0% intergenic (+82/+94) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0360A→C10.0% 52.6 ‑2.7 ‑0.4225intergenic (+82/+94)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (6/16):  ref base (98/100):  total (109/116)
Fisher's exact test strand distribution p-value = 7.01e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.55e-01

Only 100 of 237 total aligned reads displayed.

CGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGC  >  NC_014034/3003937‑3004135
                                                                                                    |                                                                                                     
 gcgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATc                                                                                                     <  1:3379091/101‑1 (MQ=255)
 gcgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATc                                                                                                     <  1:3218414/101‑1 (MQ=255)
       gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGtt                                                                                               <  2:1207900/101‑1 (MQ=255)
        cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                              <  1:87007/101‑1 (MQ=255)
        cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                              <  2:3295727/101‑1 (MQ=255)
         gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                             >  1:4284231/1‑101 (MQ=255)
           gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                           >  1:1637201/1‑101 (MQ=255)
             tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                         >  1:4175279/1‑101 (MQ=255)
             tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                         <  1:1007854/101‑1 (MQ=255)
              cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                        <  2:4216738/101‑1 (MQ=255)
               tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                       >  1:2263152/1‑101 (MQ=255)
                gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                      >  1:2955448/1‑101 (MQ=255)
                      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                <  1:2672883/101‑1 (MQ=255)
                         gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                             >  1:2175218/1‑101 (MQ=255)
                         gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                             <  2:2325717/101‑1 (MQ=255)
                          cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                            <  2:506005/101‑1 (MQ=255)
                           ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                           >  2:2409214/1‑101 (MQ=255)
                               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                       >  1:616499/1‑101 (MQ=255)
                                gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..c                                                                    <  2:801528/101‑1 (MQ=255)
                                gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..c                                                                    >  2:3355628/1‑101 (MQ=255)
                                  gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..ccc                                                                  <  2:2563074/101‑1 (MQ=255)
                                  gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..ccc                                                                  <  2:1514100/101‑1 (MQ=255)
                                  gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..ccc                                                                  <  1:3462100/101‑1 (MQ=255)
                                    tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgg                                                                >  1:3138018/1‑101 (MQ=255)
                                    tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgg                                                                >  2:2760554/1‑101 (MQ=255)
                                     gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCggg                                                               >  1:1753029/1‑101 (MQ=255)
                                      ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgggg                                                              <  2:215576/101‑1 (MQ=255)
                                      ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgggg                                                              <  1:2005716/101‑1 (MQ=255)
                                       cccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCggggg                                                             >  2:4425828/1‑101 (MQ=255)
                                         cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGa                                                           <  1:2760744/101‑1 (MQ=255)
                                         cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGa                                                           >  1:991679/1‑101 (MQ=255)
                                         cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGa                                                           <  1:1878573/101‑1 (MQ=255)
                                          ccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGCTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGCAg                                                          >  2:4069862/1‑101 (MQ=255)
                                           ctctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGt                                                         <  1:369810/101‑1 (MQ=255)
                                              ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTccc                                                      >  2:2945472/1‑101 (MQ=255)
                                                 gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCcgcg                                                   >  2:3513749/1‑101 (MQ=255)
                                                    tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCgggg                                                <  2:1092159/101‑1 (MQ=255)
                                                     gACGGCCAATTCACCCCCCGGGATATTTTGACCAAGGTGAAAGCAG.ATCCCGGGTTGCCTTTCACCCTTGCGCAAATATCCCCCGGGGGGGCCCCCGCggg                                                 >  1:792431/1‑101 (MQ=17)
                                                      aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGAc                                              <  1:706245/101‑1 (MQ=255)
                                                        ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCCG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACgg                                            >  2:605636/1‑101 (MQ=255)
                                                        ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACgg                                            >  2:3955509/1‑101 (MQ=255)
                                                        ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACgg                                            <  2:3552572/101‑1 (MQ=255)
                                                         gCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACggg                                           <  1:1288338/101‑1 (MQ=255)
                                                          ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCTTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACgggg                                          <  2:3894961/101‑1 (MQ=255)
                                                           cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACggggg                                         <  2:2235736/101‑1 (MQ=255)
                                                              ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGCGTCCCGCGGGGACGGGGGGCa                                      <  1:2544955/101‑1 (MQ=255)
                                                              ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                      >  2:4020857/1‑101 (MQ=255)
                                                               tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                     >  2:1222983/1‑101 (MQ=255)
                                                               tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTGGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                     <  1:3087476/101‑1 (MQ=255)
                                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                    <  1:2055312/99‑2 (MQ=18)
                                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                    <  1:1700433/99‑2 (MQ=18)
                                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                    <  1:2819164/99‑2 (MQ=18)
                                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                    <  1:2571983/99‑2 (MQ=18)
                                                                cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                    <  1:604306/101‑1 (MQ=255)
                                                                cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                    >  2:1453449/1‑101 (MQ=255)
                                                                 cgcccccGGGATAGTTGTCCCAAGGTGAAAGCCG.CGCCCCGGGCTCATTTCCCCTTGGCGGAACTAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                   <  2:3667713/99‑3 (MQ=18)
                                                                 cccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                   <  1:4146224/100‑3 (MQ=18)
                                                                  ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                  <  2:1107319/101‑1 (MQ=18)
                                                                  ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                  >  1:3354242/1‑101 (MQ=18)
                                                                  ccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgc                                  <  2:1936544/101‑1 (MQ=255)
                                                                   cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                 <  2:817467/101‑1 (MQ=18)
                                                                     cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                               <  2:416013/101‑1 (MQ=255)
                                                                      ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                              >  1:936755/1‑101 (MQ=255)
                                                                       cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                             >  1:1965409/1‑101 (MQ=255)
                                                                        gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                            >  1:1129132/1‑101 (MQ=255)
                                                                         ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                           >  2:3745149/1‑99 (MQ=18)
                                                                         ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                           <  1:3280179/101‑3 (MQ=17)
                                                                         ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                           <  2:1751678/101‑1 (MQ=255)
                                                                           atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                         >  2:3931986/1‑97 (MQ=18)
                                                                           atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                         >  1:399908/1‑101 (MQ=255)
                                                                            tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                        >  1:3815204/1‑101 (MQ=255)
                                                                            tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                        >  2:3279631/1‑101 (MQ=255)
                                                                             atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                       <  2:2580174/101‑7 (MQ=17)
                                                                             atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                       >  2:1649620/1‑101 (MQ=255)
                                                                              tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                      >  1:3642299/1‑101 (MQ=255)
                                                                              tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                      >  2:1350770/1‑101 (MQ=255)
                                                                               ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                     <  2:2388960/101‑1 (MQ=255)
                                                                                tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                    <  2:4351998/101‑1 (MQ=255)
                                                                                 ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                   >  1:2374229/1‑101 (MQ=255)
                                                                                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                 >  1:4359095/1‑101 (MQ=255)
                                                                                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                 >  2:195890/1‑101 (MQ=255)
                                                                                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                 <  1:983658/101‑1 (MQ=255)
                                                                                      aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc              >  1:2125251/1‑101 (MQ=255)
                                                                                      aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc              <  2:1735222/101‑1 (MQ=255)
                                                                                       aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg             >  1:3513102/1‑101 (MQ=255)
                                                                                       aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg             <  2:2869452/101‑1 (MQ=255)
                                                                                         gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg           >  1:2304393/1‑101 (MQ=255)
                                                                                           gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg         >  1:3745001/1‑101 (MQ=255)
                                                                                            aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc        >  2:3732792/1‑101 (MQ=255)
                                                                                             aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt       >  2:4230603/1‑101 (MQ=255)
                                                                                             aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt       <  1:43732/101‑1 (MQ=255)
                                                                                              aGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc      >  1:1218496/1‑101 (MQ=255)
                                                                                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg    >  2:4293725/1‑101 (MQ=255)
                                                                                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg    >  2:2193073/1‑101 (MQ=255)
                                                                                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg    <  2:1418253/101‑1 (MQ=255)
                                                                                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg    <  1:1453556/101‑1 (MQ=255)
                                                                                                 aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg   >  1:3913908/1‑101 (MQ=255)
                                                                                                 aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg   >  2:4368046/1‑101 (MQ=255)
                                                                                                 aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg   >  1:1091908/1‑101 (MQ=255)
                                                                                                  g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGc  <  1:2416094/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
CGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGC  >  NC_014034/3003937‑3004135

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG