Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,044 T→G 10.1% intergenic (+90/+86) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0440T→G10.1% 55.9 ‑3.7 ‑1.3217intergenic (+90/+86)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (6/16):  ref base (104/91):  total (110/107)
Fisher's exact test strand distribution p-value = 2.45e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.68e-02

Only 100 of 241 total aligned reads displayed.

GCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGC  >  NC_014034/3003944‑3004144
                                                                                                     |                                                                                                    
gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGtt                                                                                                      <  2:1207900/101‑1 (MQ=255)
 cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                     <  2:1424552/101‑1 (MQ=255)
  gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGAGATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                    >  1:1936673/1‑101 (MQ=255)
     aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                                 <  1:3618022/101‑1 (MQ=255)
     aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                                 <  2:356667/101‑1 (MQ=255)
      tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                                <  1:1007854/101‑1 (MQ=255)
         gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                             <  1:3411514/101‑1 (MQ=255)
         gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                             >  2:4213733/1‑101 (MQ=255)
           gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                           <  1:3643625/101‑1 (MQ=255)
             gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAAGTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                         <  2:285527/101‑1 (MQ=255)
              cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                        <  1:4282568/101‑1 (MQ=255)
              cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                        <  2:2877963/101‑1 (MQ=255)
               aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                       <  1:933005/101‑1 (MQ=255)
                           gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                           <  2:1514100/101‑1 (MQ=255)
                            cTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGAAATTTTGGCCAAGGGGAAAACAG.ATCCCGGTTTGCCTTTCACCTTGGCGCCAATATcccc                                                                          >  1:854954/1‑100 (MQ=37)
                              gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                        <  1:98028/101‑1 (MQ=255)
                              gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                        >  1:1753029/1‑101 (MQ=255)
                              gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGGATTTTGGCCCAGGGGGAAACAG.AACCCGGGTTTCCTTTCTCCCTGGGGCCAATATCCCcgg                                                                        >  1:2264607/1‑101 (MQ=12)
                               ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGCTATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                                                       >  2:4001301/1‑101 (MQ=255)
                               ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                                                       <  1:2005716/101‑1 (MQ=255)
                                cccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                      <  1:1965473/101‑1 (MQ=255)
                                  cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggggg                                                                    >  1:1512450/1‑100 (MQ=255)
                                  cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGa                                                                    >  1:991679/1‑101 (MQ=255)
                                  cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGa                                                                    <  1:2760744/101‑1 (MQ=255)
                                        tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                              >  2:1044010/1‑101 (MQ=255)
                                         ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                             >  1:3492027/1‑101 (MQ=255)
                                         ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                             >  2:342974/1‑101 (MQ=255)
                                          gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                            <  2:1398704/101‑1 (MQ=255)
                                          gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                            >  2:3513749/1‑101 (MQ=255)
                                             tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCggtg                                                         <  2:1211879/101‑3 (MQ=255)
                                             tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                         >  1:3699082/1‑101 (MQ=255)
                                                 ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCCG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                     >  2:605636/1‑101 (MQ=255)
                                                 ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                     >  2:3955509/1‑101 (MQ=255)
                                                 ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                     <  1:1103941/101‑1 (MQ=255)
                                                   ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCTTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                   <  2:3894961/101‑1 (MQ=255)
                                                      aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                >  2:3681380/1‑101 (MQ=255)
                                                       ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGCGTCCCGCGGGGACGGGGGGCa                                               <  1:2544955/101‑1 (MQ=255)
                                                        tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                              >  2:4216758/1‑101 (MQ=255)
                                                        tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTGGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                              <  1:3087476/101‑1 (MQ=255)
                                                         ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                             <  1:1700433/99‑2 (MQ=18)
                                                         ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                             <  1:2055312/99‑2 (MQ=18)
                                                         ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                             <  2:1101734/99‑2 (MQ=18)
                                                         ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                             <  1:2571983/99‑2 (MQ=18)
                                                         ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                             <  1:2819164/99‑2 (MQ=18)
                                                          cccccccGGGATATTTGGCCCAAGGTGAAAGGCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                            >  1:3951467/2‑99 (MQ=18)
                                                          aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                            >  1:3872114/1‑101 (MQ=255)
                                                           ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                           >  1:3354242/1‑101 (MQ=18)
                                                           ccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgc                                           <  2:1936544/101‑1 (MQ=255)
                                                            cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                          <  2:817467/101‑1 (MQ=18)
                                                             ccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                         >  2:4061784/1‑101 (MQ=255)
                                                              cccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccc                                        >  2:2205486/1‑101 (MQ=18)
                                                              cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                        <  2:416013/101‑1 (MQ=255)
                                                               ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                       >  1:2987902/1‑101 (MQ=18)
                                                               ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                       >  1:3207308/1‑101 (MQ=18)
                                                               ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                       <  2:240473/101‑1 (MQ=255)
                                                                cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                      <  2:2360672/101‑1 (MQ=17)
                                                                cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                      <  1:643344/101‑1 (MQ=255)
                                                                 gggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccc                                     >  2:702683/1‑100 (MQ=18)
                                                                 gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                     <  1:723588/101‑1 (MQ=255)
                                                                  ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                    >  2:3745149/1‑99 (MQ=18)
                                                                  ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                    <  1:3280179/101‑3 (MQ=17)
                                                                  ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                    <  2:480584/101‑3 (MQ=17)
                                                                   gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                   >  1:4164314/1‑101 (MQ=255)
                                                                    atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                  >  1:1214952/1‑97 (MQ=18)
                                                                    atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                  >  2:3931986/1‑97 (MQ=18)
                                                                     tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                 >  2:3279631/1‑101 (MQ=255)
                                                                     tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccaccctgccc                                 >  1:2215341/1‑101 (MQ=255)
                                                                      atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                >  2:1649620/1‑101 (MQ=255)
                                                                       tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                               >  2:1350770/1‑101 (MQ=255)
                                                                          ggANCNAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                            <  1:1238509/101‑1 (MQ=255)
                                                                          ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                            >  1:2374229/1‑101 (MQ=255)
                                                                            aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                          >  1:4359095/1‑101 (MQ=255)
                                                                            aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                          >  2:195890/1‑101 (MQ=255)
                                                                            aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                          <  1:605704/101‑1 (MQ=255)
                                                                             ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                         <  2:2190830/101‑1 (MQ=255)
                                                                              cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                        <  2:4385826/101‑1 (MQ=255)
                                                                              cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                        >  2:3303478/1‑101 (MQ=255)
                                                                               aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                       >  1:2125251/1‑101 (MQ=255)
                                                                               aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                       <  2:1735222/101‑1 (MQ=255)
                                                                                 ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                     >  2:2568701/1‑101 (MQ=255)
                                                                                  gggAAAGCAG.ATCCGGGTTTGCCTTCCCCCTTGTGGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                    <  1:3799953/99‑1 (MQ=21)
                                                                                   tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                   <  2:2464340/101‑1 (MQ=255)
                                                                                     aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAgcgc                 >  1:4133815/1‑101 (MQ=255)
                                                                                     aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                 >  2:270587/1‑101 (MQ=255)
                                                                                     aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                 >  1:2741127/1‑101 (MQ=255)
                                                                                         cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg             <  1:1453556/101‑1 (MQ=255)
                                                                                         cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg             >  1:59041/1‑101 (MQ=255)
                                                                                         cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg             >  2:4293725/1‑101 (MQ=255)
                                                                                          aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg            >  2:4368046/1‑101 (MQ=255)
                                                                                           g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGc           <  1:2416094/101‑1 (MQ=255)
                                                                                           g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc           <  2:2313756/101‑1 (MQ=255)
                                                                                           g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc           >  2:1761110/1‑101 (MQ=255)
                                                                                              tCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt         <  2:854872/101‑1 (MQ=255)
                                                                                              tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCACGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt         >  2:3859693/1‑101 (MQ=255)
                                                                                               cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg        >  1:163648/1‑101 (MQ=255)
                                                                                                 cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt      >  2:1548536/1‑101 (MQ=255)
                                                                                                 cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt      >  2:314446/1‑101 (MQ=255)
                                                                                                  ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt     >  1:2304866/1‑101 (MQ=255)
                                                                                                   gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg    >  2:4329255/1‑101 (MQ=255)
                                                                                                    tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg   >  1:1061729/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGC  >  NC_014034/3003944‑3004144

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG