Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,048 C→A 12.2% intergenic (+94/+82) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0480C→A12.2% 65.8 ‑1.2 ‑0.2222intergenic (+94/+82)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (11/16):  ref base (102/92):  total (113/109)
Fisher's exact test strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.40e-01

Only 100 of 240 total aligned reads displayed.

GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148
                                                                                                      |                                                                                                    
gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                      <  1:4209067/101‑1 (MQ=255)
 aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                                     <  2:356667/101‑1 (MQ=255)
  tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                                    >  2:1951497/1‑101 (MQ=255)
  tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                                    <  1:1007854/101‑1 (MQ=255)
   cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                                   <  2:4216738/101‑1 (MQ=255)
    tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                                  >  1:2263152/1‑101 (MQ=255)
     gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                                 <  1:3411514/101‑1 (MQ=255)
       gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                               <  1:3643625/101‑1 (MQ=255)
        ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                              <  1:214352/101‑1 (MQ=255)
         gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAAGTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                             <  2:285527/101‑1 (MQ=255)
           aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                           <  1:933005/101‑1 (MQ=255)
           aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                           <  1:2058353/101‑1 (MQ=255)
             ggCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcg                                                                                         <  1:2806174/101‑1 (MQ=255)
              gCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                        <  2:2325717/101‑1 (MQ=255)
               cGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                                       <  2:506005/101‑1 (MQ=255)
                 gggggCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                     <  1:266991/101‑1 (MQ=255)
                     gcgcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                                 <  2:801528/101‑1 (MQ=255)
                     gcgcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                                 >  2:3355628/1‑101 (MQ=255)
                     gcgcTCCCCCCCTC.TGGCCTGGACGGCCATTTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                                 <  2:1272517/101‑1 (MQ=21)
                       gcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                               <  2:1514100/101‑1 (MQ=255)
                        cTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGAAATTTTGGCCAAGGGGAAAACAG.ATCCCGGTTTGCCTTTCACCTTGGCGCCAATATcccc                                                                              >  1:854954/1‑100 (MQ=37)
                         tGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                             >  1:2464497/1‑101 (MQ=255)
                         tGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                             >  1:3138018/1‑101 (MQ=255)
                          gCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                            >  1:1753029/1‑101 (MQ=255)
                           ccccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCgggg                                                                           <  2:3432508/101‑1 (MQ=255)
                            cccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                          >  2:2051590/1‑101 (MQ=255)
                            cccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                          >  1:3945728/1‑101 (MQ=255)
                              cccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggggg                                                                        >  1:1512450/1‑100 (MQ=255)
                              cccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGa                                                                        >  1:991679/1‑101 (MQ=255)
                               ccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGCTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGCAg                                                                       >  2:4069862/1‑101 (MQ=255)
                                 tctTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGTGGAGTc                                                                     <  2:1356930/101‑1 (MQ=255)
                                   ttGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                   >  2:2945472/1‑101 (MQ=255)
                                     ggCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                 >  1:3492027/1‑101 (MQ=255)
                                         t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                             <  2:1092159/101‑1 (MQ=255)
                                         t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                             >  1:3699082/1‑101 (MQ=255)
                                             cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                          <  1:282944/101‑1 (MQ=255)
                                              ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                         <  1:1103941/101‑1 (MQ=255)
                                                ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCTTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                       <  2:3894961/101‑1 (MQ=255)
                                                   aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    <  2:3287063/101‑1 (MQ=255)
                                                    ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                   >  2:4020857/1‑101 (MQ=255)
                                                     tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTGGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                  <  1:3087476/101‑1 (MQ=255)
                                                      ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                 <  1:1700433/99‑2 (MQ=18)
                                                      ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                 <  2:3549841/99‑2 (MQ=18)
                                                      ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                 <  1:2571983/99‑2 (MQ=18)
                                                      ccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                 <  2:4283962/99‑1 (MQ=255)
                                                      cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                 >  2:1453449/1‑101 (MQ=255)
                                                       cccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                <  1:4146224/100‑3 (MQ=18)
                                                       cccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                <  2:4167849/100‑3 (MQ=18)
                                                       aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                >  1:3872114/1‑101 (MQ=255)
                                                       aCCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.ATCCCGGGTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGGCCCGCGGGGACGGGGGGCaggg                                                >  2:845948/1‑99 (MQ=21)
                                                        ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                               <  2:1107319/101‑1 (MQ=18)
                                                        ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                               >  1:3354242/1‑101 (MQ=18)
                                                         cccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                              >  2:1210635/1‑101 (MQ=255)
                                                            ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                           >  1:2987902/1‑101 (MQ=18)
                                                            ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACGTTGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                           <  1:2039744/101‑1 (MQ=255)
                                                             cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                          >  1:1965409/1‑101 (MQ=255)
                                                              gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                         <  1:723588/101‑1 (MQ=255)
                                                               ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                        >  2:394391/1‑99 (MQ=18)
                                                               ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                        >  2:3745149/1‑99 (MQ=18)
                                                               ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                        >  2:2280410/1‑99 (MQ=18)
                                                               ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                        <  1:3280179/101‑3 (MQ=17)
                                                                 atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                      >  1:1214952/1‑97 (MQ=18)
                                                                   atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                    <  2:2580174/101‑7 (MQ=17)
                                                                   atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                    >  2:1649620/1‑101 (MQ=255)
                                                                    tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                                   >  2:4000102/1‑101 (MQ=255)
                                                                     ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                  <  2:2388960/101‑1 (MQ=255)
                                                                      tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                 <  2:4351998/101‑1 (MQ=255)
                                                                       ggANCNAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                <  1:1238509/101‑1 (MQ=255)
                                                                         aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                              <  1:605704/101‑1 (MQ=255)
                                                                         aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                              <  1:983658/101‑1 (MQ=255)
                                                                         aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                              >  1:4359095/1‑101 (MQ=255)
                                                                          ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                             <  2:2190830/101‑1 (MQ=255)
                                                                           cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                            <  1:623003/101‑1 (MQ=255)
                                                                           cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                            <  2:4385826/101‑1 (MQ=255)
                                                                            aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                           <  2:1735222/101‑1 (MQ=255)
                                                                               gggAAAGCAG.ATCCGGGTTTGCCTTCCCCCTTGTGGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                        <  1:3799953/99‑1 (MQ=21)
                                                                               gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                        >  1:2304393/1‑101 (MQ=255)
                                                                                tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                       <  2:2464340/101‑1 (MQ=255)
                                                                                  aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                     >  2:270587/1‑101 (MQ=255)
                                                                                  aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                     >  2:3732792/1‑101 (MQ=255)
                                                                                  aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                     >  1:2741127/1‑101 (MQ=255)
                                                                                   aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGCGCGCt                    >  1:353243/1‑101 (MQ=255)
                                                                                   aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                    <  1:43732/101‑1 (MQ=255)
                                                                                      cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                 <  1:1453556/101‑1 (MQ=255)
                                                                                       aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  2:4368046/1‑101 (MQ=255)
                                                                                       aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  1:2317422/1‑101 (MQ=255)
                                                                                       aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  1:1972050/1‑101 (MQ=255)
                                                                                        g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGc               <  1:2416094/101‑1 (MQ=255)
                                                                                        g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               >  2:1761110/1‑101 (MQ=255)
                                                                                        g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               <  2:616431/101‑1 (MQ=255)
                                                                                           tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCACGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt             >  2:3859693/1‑101 (MQ=255)
                                                                                            cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg            >  1:163648/1‑101 (MQ=255)
                                                                                              cGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt          <  2:4322873/101‑1 (MQ=255)
                                                                                              cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt          >  2:1548536/1‑101 (MQ=255)
                                                                                              cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt          >  2:314446/1‑101 (MQ=255)
                                                                                                 tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       >  1:1061729/1‑101 (MQ=255)
                                                                                                   tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg     <  1:4021114/101‑1 (MQ=255)
                                                                                                    gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa    >  2:2193844/1‑101 (MQ=255)
                                                                                                      cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc  >  2:2411980/1‑101 (MQ=255)
                                                                                                      cTTTAACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCATGTCGGGCGGGc  >  2:909311/1‑101 (MQ=25)
                                                                                                      |                                                                                                    
GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG