Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,053 A→C 13.3% intergenic (+99/+77) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0530A→C13.3% 74.0 ‑2.5 ‑1.4223intergenic (+99/+77)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (11/19):  ref base (105/88):  total (116/107)
Fisher's exact test strand distribution p-value = 7.94e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.75e-02

Only 100 of 237 total aligned reads displayed.

GAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCG  >  NC_014034/3003953‑3004150
                                                                                                     |                                                                                                   
gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCa                                                                                                     >  2:4213733/1‑101 (MQ=255)
gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCa                                                                                                     >  1:2955448/1‑101 (MQ=255)
 aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCAc                                                                                                    >  2:1747296/1‑101 (MQ=255)
    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAAGTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCtt                                                                                                 <  2:285527/101‑1 (MQ=255)
     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTg                                                                                                <  2:2877963/101‑1 (MQ=255)
      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTgg                                                                                               <  1:933005/101‑1 (MQ=255)
        ggCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGgcg                                                                                             <  1:2806174/101‑1 (MQ=255)
         gCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                            <  2:2325717/101‑1 (MQ=255)
          cGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                                           <  2:506005/101‑1 (MQ=255)
           ggggggCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGGaa                                                                                          >  1:992732/1‑101 (MQ=255)
           ggggggCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                                          >  2:2409214/1‑101 (MQ=255)
            gggggCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                         <  1:266991/101‑1 (MQ=255)
               ggCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                      <  2:4358768/101‑1 (MQ=255)
               ggCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                      <  1:328323/101‑1 (MQ=255)
                gcgcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..c                                                                                   <  2:801528/101‑1 (MQ=255)
                gcgcTCCCCCCCTC.TGGCCTGGACGGCCATTTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..c                                                                                   <  2:1272517/101‑1 (MQ=21)
                  gcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..ccc                                                                                 <  2:1514100/101‑1 (MQ=255)
                  gcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..ccc                                                                                 <  1:3462100/101‑1 (MQ=255)
                    tGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgg                                                                               >  1:2464497/1‑101 (MQ=255)
                      ccccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGCTATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgggg                                                                             >  2:4001301/1‑101 (MQ=255)
                      ccccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCCCCTTGGCGCAAATAT..CCCgggg                                                                             <  2:3432508/101‑1 (MQ=255)
                      ccccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgggg                                                                             <  2:215576/101‑1 (MQ=255)
                       cccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCggggg                                                                            <  1:1965473/101‑1 (MQ=255)
                       cccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCggggg                                                                            >  2:2051590/1‑101 (MQ=255)
                         cccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGa                                                                          <  1:1878573/101‑1 (MQ=255)
                            tctTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGTGGAGTc                                                                       <  2:1356930/101‑1 (MQ=255)
                                 gCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCcgcg                                                                  >  2:3513749/1‑101 (MQ=255)
                                    t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCggtg                                                               <  2:1211879/101‑3 (MQ=255)
                                    t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCgggg                                                               <  2:1092159/101‑1 (MQ=255)
                                    t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCgggg                                                               >  1:3699082/1‑101 (MQ=255)
                                      gACGGCCAATTCACCCCCCGGGATATTTTGACCAAGGTGAAAGCAGATCCCGGGTTGCCTTTCACCCTTGCGCAAATATCCCCCGGGGGGGCCCCCGCggg                                                                >  1:792431/1‑101 (MQ=17)
                                        cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACg                                                            <  1:282944/101‑1 (MQ=255)
                                          gCCATTTCCCCCCCGGGGATATTTGGACCAGGGTAAAAGCAGATCCCGGTTTGCCTTTCCCCTTGGCGAAAATAC..CCCGGGGGGAGTCCCGCGGGGACggg                                                          <  2:3738466/101‑1 (MQ=21)
                                          gCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACggg                                                          <  1:1288338/101‑1 (MQ=255)
                                            cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACggggg                                                        <  2:2235736/101‑1 (MQ=255)
                                              aTTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                      <  2:3858883/101‑1 (MQ=255)
                                              aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                      >  2:3681380/1‑101 (MQ=255)
                                               ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                     >  1:3367320/1‑101 (MQ=255)
                                                tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                    >  1:1551395/1‑101 (MQ=255)
                                                 ccccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                   <  2:3549841/99‑2 (MQ=18)
                                                 ccccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                   <  1:2055312/99‑2 (MQ=18)
                                                 ccccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                   <  1:2571983/99‑2 (MQ=18)
                                                 cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                   >  2:1453449/1‑101 (MQ=255)
                                                  cccccccGGGATATTTGGCCCAAGGTGAAAGGCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                  >  1:3951467/2‑99 (MQ=18)
                                                  aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                  >  2:2663500/1‑101 (MQ=255)
                                                  aCCCCCCGGGATATTTGGACCAAGGGGAAAGCAGATCCCGGGTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGGGGCCCGCGGGGACGGGGGGCaggg                                                  >  2:845948/1‑99 (MQ=21)
                                                   ccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                 <  2:1107319/101‑1 (MQ=18)
                                                   ccccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgc                                                 <  2:1936544/101‑1 (MQ=255)
                                                     ccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                               >  2:4061784/1‑101 (MQ=255)
                                                      cccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                              <  2:416013/101‑1 (MQ=255)
                                                       ccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                             >  1:3207308/1‑101 (MQ=18)
                                                       ccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACGTTGGCGAAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                             <  1:2039744/101‑1 (MQ=255)
                                                        cGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                            <  1:1046358/101‑1 (MQ=17)
                                                         gggATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                           >  1:1129132/1‑101 (MQ=255)
                                                           gATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                         >  1:4164314/1‑101 (MQ=255)
                                                            atatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                        >  2:3931986/1‑97 (MQ=18)
                                                             tatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                       >  2:3279631/1‑101 (MQ=255)
                                                             tatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                       >  1:3815204/1‑101 (MQ=255)
                                                             tatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccaccctgccc                                       >  1:2215341/1‑101 (MQ=255)
                                                              atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCCGAgcccccctgcccc                                      >  2:1576894/1‑101 (MQ=255)
                                                              atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                      >  2:1649620/1‑101 (MQ=255)
                                                               tttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                                     >  1:3642299/1‑101 (MQ=255)
                                                                ttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                    <  2:2388960/101‑1 (MQ=255)
                                                                   gACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                 >  1:3793567/1‑101 (MQ=255)
                                                                    aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                <  1:983658/101‑1 (MQ=255)
                                                                    aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                >  1:4359095/1‑101 (MQ=255)
                                                                    aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                <  1:605704/101‑1 (MQ=255)
                                                                      cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                              >  2:3303478/1‑101 (MQ=255)
                                                                      cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                              <  1:623003/101‑1 (MQ=255)
                                                                      cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                              >  1:3120553/1‑101 (MQ=255)
                                                                      cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                              <  2:4385826/101‑1 (MQ=255)
                                                                       aaGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                             >  1:2125251/1‑101 (MQ=255)
                                                                        aGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                            >  1:3513102/1‑101 (MQ=255)
                                                                         ggTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                           >  2:2568701/1‑101 (MQ=255)
                                                                          gTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                          >  1:2304393/1‑101 (MQ=255)
                                                                           tGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                         <  2:2464340/101‑1 (MQ=255)
                                                                             aaaGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAgcgc                       >  1:4133815/1‑101 (MQ=255)
                                                                             aaaGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                       >  1:2741127/1‑101 (MQ=255)
                                                                              aaGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                      >  2:4230603/1‑101 (MQ=255)
                                                                              aaGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                      <  1:1699609/101‑1 (MQ=255)
                                                                                 cAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                   >  1:59041/1‑101 (MQ=255)
                                                                                 cAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                   <  1:1453556/101‑1 (MQ=255)
                                                                                 cAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAC..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                   <  1:1747415/101‑1 (MQ=255)
                                                                                  aGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                  >  2:4368046/1‑101 (MQ=255)
                                                                                  aGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                  >  1:2317422/1‑101 (MQ=255)
                                                                                  aGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                  >  1:1091908/1‑101 (MQ=255)
                                                                                   gATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGc                 <  1:2416094/101‑1 (MQ=255)
                                                                                   gATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                 <  2:616431/101‑1 (MQ=255)
                                                                                        cGGTTTGCCTTTCCCCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt            <  2:4322873/101‑1 (MQ=255)
                                                                                        cGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt            >  2:314446/1‑101 (MQ=255)
                                                                                          gTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg          <  2:3871861/101‑1 (MQ=255)
                                                                                            ttGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc        <  2:3964029/101‑1 (MQ=255)
                                                                                              gCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa      >  2:2193844/1‑101 (MQ=255)
                                                                                                cTTTAACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCATGTCGGGCGGGc    >  2:909311/1‑101 (MQ=25)
                                                                                                 tttCGCCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc   <  1:1223076/101‑1 (MQ=255)
                                                                                                 tttCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGGCGAGCCCCCCTCCCCCCCCGGGGCCGAGCGCCCAGGGGTGGTTTGCGAggc   >  1:945665/1‑99 (MQ=21)
                                                                                                 tttCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc   >  1:722122/1‑101 (MQ=255)
                                                                                                 tttCACCTTGGCGCAAATAT..CCCGGGGGGACTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGGGCGCTCAGGCGTGTTTGGGGAGcc   >  2:4301326/1‑101 (MQ=255)
                                                                                                  ttCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg  >  1:3069488/1‑101 (MQ=255)
                                                                                                  ttCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg  >  1:3933187/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
GAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCG  >  NC_014034/3003953‑3004150

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG