Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,062 C→G 12.4% intergenic (+108/+68) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0620C→G12.4% 64.6 ‑1.9 ‑0.8218intergenic (+108/+68)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (11/16):  ref base (109/82):  total (120/98)
Fisher's exact test strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.71e-01

Only 100 of 240 total aligned reads displayed.

GCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAC  >  NC_014034/3003962‑3004162
                                                                                                      |                                                                                                      
gCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                                        <  2:2325717/101‑1 (MQ=255)
   gggggCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                                     <  1:266991/101‑1 (MQ=255)
       gcgcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..c                                                                                               <  2:801528/101‑1 (MQ=255)
       gcgcTCCCCCCCTC.TGGCCTGGACGGCCATTTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..c                                                                                               <  2:1272517/101‑1 (MQ=21)
        cgcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..cc                                                                                              <  2:1156751/101‑1 (MQ=255)
         gcTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..ccc                                                                                             <  2:3863357/101‑1 (MQ=255)
           tGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgg                                                                                           >  1:2464497/1‑101 (MQ=255)
            gCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTTACCTTTGCGCAAATAT..CCCggg                                                                                          >  1:989187/1‑101 (MQ=255)
            gCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCggg                                                                                          <  1:98028/101‑1 (MQ=255)
            gCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCggg                                                                                          >  1:1753029/1‑101 (MQ=255)
             ccccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATAT..CCCgggg                                                                                         <  2:3432508/101‑1 (MQ=255)
             ccccccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCgggg                                                                                         <  2:215576/101‑1 (MQ=255)
                cccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGa                                                                                      <  1:1878573/101‑1 (MQ=255)
                cccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGa                                                                                      <  1:2760744/101‑1 (MQ=255)
                 ccTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGCTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGCAg                                                                                     >  2:4069862/1‑101 (MQ=255)
                       ggCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCcgc                                                                               >  1:3492027/1‑101 (MQ=255)
                        gCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCcgcg                                                                              <  2:1398704/101‑1 (MQ=255)
                        gCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCcgcg                                                                              >  2:3513749/1‑101 (MQ=255)
                           t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCggtg                                                                           <  2:1211879/101‑3 (MQ=255)
                           t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCgggg                                                                           >  1:3699082/1‑101 (MQ=255)
                           t.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCgggg                                                                           <  2:1092159/101‑1 (MQ=255)
                             gACGGCCAATTCACCCCCCGGGATATTTTGACCAAGGTGAAAGCAG.ATCCCGGGTTGCCTTTCACCCTTGCGCAAATATCCCCCGGGGGGGCCCCCGCggg                                                                            >  1:792431/1‑101 (MQ=17)
                               cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACg                                                                        <  1:282944/101‑1 (MQ=255)
                                 gCCATTTCCCCCCCGGGGATATTTGGACCAGGGTAAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGAAAATAC..CCCGGGGGGAGTCCCGCGGGGACggg                                                                      <  2:3738466/101‑1 (MQ=21)
                                 gCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACggg                                                                      <  1:1288338/101‑1 (MQ=255)
                                     aTTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                                  <  2:3858883/101‑1 (MQ=255)
                                     aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                                  >  2:3681380/1‑101 (MQ=255)
                                      ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                                 >  2:4020857/1‑101 (MQ=255)
                                       tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                >  1:1551395/1‑101 (MQ=255)
                                        ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                               <  1:2571983/99‑2 (MQ=18)
                                        ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                               <  2:3549841/99‑2 (MQ=18)
                                        ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                               <  1:2055312/99‑2 (MQ=18)
                                        cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                               >  2:1453449/1‑101 (MQ=255)
                                         cccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                              <  1:4146224/100‑3 (MQ=18)
                                         aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                              >  1:3872114/1‑101 (MQ=255)
                                          ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                             >  1:3354242/1‑101 (MQ=18)
                                           cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                                            <  2:817467/101‑1 (MQ=18)
                                             cccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccc                                                          >  2:2205486/1‑101 (MQ=18)
                                             cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                                          <  2:416013/101‑1 (MQ=255)
                                              ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACGTTGGCGAAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                         <  1:2039744/101‑1 (MQ=255)
                                              ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                         >  1:936755/1‑101 (MQ=255)
                                              ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                         <  2:240473/101‑1 (MQ=255)
                                               cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                                        <  1:1046358/101‑1 (MQ=17)
                                                gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                                       >  1:1129132/1‑101 (MQ=255)
                                                 ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                      >  2:394391/1‑99 (MQ=18)
                                                 ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                      >  2:2280410/1‑99 (MQ=18)
                                                 ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                      <  2:480584/101‑3 (MQ=17)
                                                 ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                      <  1:3280179/101‑3 (MQ=17)
                                                   atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                                    >  1:399908/1‑101 (MQ=255)
                                                    tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                                   >  2:3279631/1‑101 (MQ=255)
                                                    tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccaccctgccc                                                   >  1:2215341/1‑101 (MQ=255)
                                                     atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATAT..CCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                                  <  2:2580174/101‑7 (MQ=17)
                                                     atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCCGAgcccccctgcccc                                                  >  2:1576894/1‑101 (MQ=255)
                                                     atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                                  >  2:1649620/1‑101 (MQ=255)
                                                      tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                                                 >  1:3642299/1‑101 (MQ=255)
                                                        tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                               <  2:4351998/101‑1 (MQ=255)
                                                          gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                             >  1:3793567/1‑101 (MQ=255)
                                                           aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                            >  1:4359095/1‑101 (MQ=255)
                                                           aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                            >  2:195890/1‑101 (MQ=255)
                                                           aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                            <  1:605704/101‑1 (MQ=255)
                                                             cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                          <  2:4385826/101‑1 (MQ=255)
                                                             cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                          >  1:3120553/1‑101 (MQ=255)
                                                             cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                          >  2:3303478/1‑101 (MQ=255)
                                                             cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                          <  1:623003/101‑1 (MQ=255)
                                                                 gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                                      >  1:2304393/1‑101 (MQ=255)
                                                                  tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                     <  2:2464340/101‑1 (MQ=255)
                                                                    aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                                   >  2:270587/1‑101 (MQ=255)
                                                                     aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                  <  1:1699609/101‑1 (MQ=255)
                                                                     aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                  >  2:4230603/1‑101 (MQ=255)
                                                                     aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                  <  1:43732/101‑1 (MQ=255)
                                                                        cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                               >  1:59041/1‑101 (MQ=255)
                                                                        cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                               >  2:4293725/1‑101 (MQ=255)
                                                                        cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                               >  2:2193073/1‑101 (MQ=255)
                                                                         aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                              >  1:1091908/1‑101 (MQ=255)
                                                                         aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                              >  1:1972050/1‑101 (MQ=255)
                                                                          g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGc                             <  1:2416094/101‑1 (MQ=255)
                                                                          g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                             <  2:616431/101‑1 (MQ=255)
                                                                          g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                             >  2:1761110/1‑101 (MQ=255)
                                                                             tCCCGGTTTGCCTTTCCCCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                           <  2:854872/101‑1 (MQ=255)
                                                                                cGGTTTGCCTTTCCCCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                        <  2:4322873/101‑1 (MQ=255)
                                                                                cGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                        >  2:314446/1‑101 (MQ=255)
                                                                                  gTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGGGCGCTCAGGCGTGGTTg                      >  2:1516121/1‑101 (MQ=255)
                                                                                  gTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                      <  2:3871861/101‑1 (MQ=255)
                                                                                   tttGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                     >  1:1509788/1‑101 (MQ=255)
                                                                                    ttGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                    >  2:955019/1‑101 (MQ=255)
                                                                                    ttGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                    <  2:3964029/101‑1 (MQ=255)
                                                                                    ttGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                    >  1:2336690/1‑101 (MQ=255)
                                                                                     tGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                   >  2:4365124/1‑101 (MQ=255)
                                                                                      gCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa                  <  1:2945693/101‑1 (MQ=255)
                                                                                        cTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc                >  2:2411980/1‑101 (MQ=255)
                                                                                         tttCGCCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc               <  1:1223076/101‑1 (MQ=255)
                                                                                         tttCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc               >  1:722122/1‑101 (MQ=255)
                                                                                          ttCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg              >  1:3069488/1‑101 (MQ=255)
                                                                                          ttCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg              >  1:3933187/1‑101 (MQ=255)
                                                                                            caacTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGct            >  2:3130760/4‑101 (MQ=255)
                                                                                              ccTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct          >  1:3406820/1‑101 (MQ=255)
                                                                                               cTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt         >  2:1018497/1‑101 (MQ=255)
                                                                                                ttGGCGCAAATAT..CCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTc        <  1:4060964/101‑1 (MQ=255)
                                                                                                    cgcAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc    >  1:3245239/1‑101 (MQ=255)
                                                                                                     gcAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCa   >  2:289395/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
GCGGGGGGCGCTGCCCCCCTCTTGGCCT.GACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATAT..CCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAC  >  NC_014034/3003962‑3004162

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG