Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,086 G→A 12.8% intergenic (+132/+44) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0860G→A12.8% 76.4 ‑3.2 ‑1.3219intergenic (+132/+44)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (11/17):  ref base (117/74):  total (128/91)
Fisher's exact test strand distribution p-value = 3.89e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.48e-02

Only 100 of 241 total aligned reads displayed.

GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGT  >  NC_014034/3003986‑3004185
                                                                                                     |                                                                                                   
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCggtg                                                                                                  <  2:1211879/101‑3 (MQ=255)
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                  >  1:3699082/1‑101 (MQ=255)
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                  <  2:1092159/101‑1 (MQ=255)
      cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                                                               <  1:282944/101‑1 (MQ=255)
       ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                                                              <  2:3552572/101‑1 (MQ=255)
       ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                                                              >  2:3955509/1‑101 (MQ=255)
        gCCATTTCCCCCCCGGGGATATTTGGACCAGGGTAAAAGCAGATCCCGGTTTGCCTTTCCCCTTGG.CGAAAATACCCCGGGGGGAGTCCCGCGGGGACggg                                                                                             <  2:3738466/101‑1 (MQ=21)
         ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCTTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                                                            <  2:3894961/101‑1 (MQ=255)
          cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                                                           <  2:2235736/101‑1 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                          >  1:2746563/1‑101 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                          <  2:811957/101‑1 (MQ=255)
            aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                                                         >  2:3681380/1‑101 (MQ=255)
             ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                                                        >  1:3367320/1‑101 (MQ=255)
              tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                                       <  1:897605/101‑1 (MQ=255)
              tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                                       >  2:4216758/1‑101 (MQ=255)
               cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                                                      <  1:604306/101‑1 (MQ=255)
               cACCCCCCGGGATATTTGGACCAAGGTGAAAGCACATCGCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGGGTCCCGCGGGGTCCGGGGGTaga                                                                                      >  2:1494995/1‑101 (MQ=25)
                cccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGG.CGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                                                     <  1:4146224/100‑3 (MQ=18)
                aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                     >  1:3872114/1‑101 (MQ=255)
                aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                     >  2:2663500/1‑101 (MQ=255)
                 ccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGG.CGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                                                    >  1:3354242/1‑101 (MQ=18)
                 ccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGG.CGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                                                    <  2:1107319/101‑1 (MQ=18)
                 ccccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgc                                                                                    >  2:43719/1‑101 (MQ=255)
                  cccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                                                                   <  1:3260845/101‑1 (MQ=255)
                   ccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                                                                  >  2:4061784/1‑101 (MQ=255)
                     ccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACGTTGG.CGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                                                <  1:2039744/101‑1 (MQ=255)
                     ccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                                                <  2:240473/101‑1 (MQ=255)
                      cGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGG.CGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                                                               <  2:2360672/101‑1 (MQ=17)
                      cGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                                                               >  1:1965409/1‑101 (MQ=255)
                      cGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                                                               <  1:643344/101‑1 (MQ=255)
                        ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGG.CGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                                             <  2:480584/101‑3 (MQ=17)
                        ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGG.CGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                                             <  1:1765135/101‑3 (MQ=17)
                          atatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                                                           >  1:399908/1‑101 (MQ=255)
                             tttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                                                                        >  2:1350770/1‑101 (MQ=255)
                             tttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                                                                        >  2:4000102/1‑101 (MQ=255)
                               tGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                                                      <  2:4351998/101‑1 (MQ=255)
                                 gACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                                                    >  1:3793567/1‑101 (MQ=255)
                                 gACCAAGGTGAAAGCAGATCCAGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                                                    >  1:2548354/1‑101 (MQ=255)
                                  aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                   >  2:195890/1‑101 (MQ=255)
                                    cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                 >  2:3303478/1‑101 (MQ=255)
                                    cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                 >  1:3120553/1‑101 (MQ=255)
                                     aaGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                                                <  2:1735222/101‑1 (MQ=255)
                                     aaGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                                                >  1:2125251/1‑101 (MQ=255)
                                      aGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                                                               >  1:3513102/1‑101 (MQ=255)
                                      aGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                                                               <  2:2869452/101‑1 (MQ=255)
                                       ggTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                                                              >  2:2568701/1‑101 (MQ=255)
                                         tGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                            <  2:326873/101‑1 (MQ=255)
                                            aaGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                                         <  1:1699609/101‑1 (MQ=255)
                                             aGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc                                                        >  1:1218496/1‑101 (MQ=255)
                                              gCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                                                       >  1:4372584/1‑101 (MQ=255)
                                               cAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                      <  1:1453556/101‑1 (MQ=255)
                                               cAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                      <  2:1418253/101‑1 (MQ=255)
                                                aGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                                                     >  1:3913908/1‑101 (MQ=255)
                                                 gATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                                                    >  2:1761110/1‑101 (MQ=255)
                                                 gATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                                                    <  2:616431/101‑1 (MQ=255)
                                                    cccGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                                                 >  1:163648/1‑101 (MQ=255)
                                                      cGGTTTGCCTTTCCCCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                               <  2:4322873/101‑1 (MQ=255)
                                                      cGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                               >  2:314446/1‑101 (MQ=255)
                                                      cGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                               >  2:1548536/1‑101 (MQ=255)
                                                       ggTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt                                              >  1:2304866/1‑101 (MQ=255)
                                                         tttGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                            >  1:1061729/1‑101 (MQ=255)
                                                          ttGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                                           >  1:2336690/1‑101 (MQ=255)
                                                          ttGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                                           >  2:955019/1‑101 (MQ=255)
                                                           tGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                                          >  1:2586525/1‑101 (MQ=255)
                                                            gCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa                                         >  2:2193844/1‑101 (MQ=255)
                                                            gCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa                                         <  1:2945693/101‑1 (MQ=255)
                                                             ccTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg                                        <  2:2142160/101‑1 (MQ=255)
                                                             ccTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg                                        >  2:3543630/1‑101 (MQ=255)
                                                               tttCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGGCGAGCCCCCCTCCCCCCCCGGGGCCGAGCGCCCAGGGGTGGTTTGCGAggc                                      >  1:945665/1‑99 (MQ=21)
                                                               tttCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                      >  1:722122/1‑101 (MQ=255)
                                                               tttCACCTTGG.CGCAAATATCCCGGGGGGACTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGGGCGCTCAGGCGTGTTTGGGGAGcc                                      >  2:4301326/1‑101 (MQ=255)
                                                                ttCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg                                     <  2:3462235/101‑1 (MQ=255)
                                                                ttCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg                                     >  1:3069488/1‑101 (MQ=255)
                                                                  tcccTTGGCCGCAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCTCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCgg                                    <  2:3858360/99‑2 (MQ=37)
                                                                  caacTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGct                                   >  2:3130760/4‑101 (MQ=255)
                                                                    ccTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct                                 >  1:3406820/1‑101 (MQ=255)
                                                                      ttGG.CGCAAATGTCCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTc                               <  2:3866100/101‑1 (MQ=255)
                                                                      ttGG.CGCAAATATCCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTc                               <  1:4060964/101‑1 (MQ=255)
                                                                        gg.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGa                             >  2:2264041/1‑101 (MQ=255)
                                                                           cgcAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                           >  2:853222/1‑101 (MQ=255)
                                                                           cgcAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                           >  1:4444131/1‑101 (MQ=255)
                                                                           cgcAAATACCCGGGGGGGAGCCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                           <  1:4332093/101‑1 (MQ=255)
                                                                               aaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                       >  1:115809/1‑101 (MQ=255)
                                                                               aaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                       >  1:3650333/1‑101 (MQ=255)
                                                                               aaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                       >  2:4246860/1‑101 (MQ=255)
                                                                                atatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGAGTTTGGCGAGCCGCTCTTCGAGCACGTc                      >  1:1302637/1‑101 (MQ=255)
                                                                                 tatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCg                     >  2:4398246/1‑101 (MQ=255)
                                                                                 tatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGCCg                     >  1:1286627/1‑101 (MQ=255)
                                                                                 taccccGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCg                     <  1:2409371/98‑1 (MQ=255)
                                                                                  atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                    >  1:749866/1‑101 (MQ=255)
                                                                                    cccGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAg                  >  1:241919/1‑101 (MQ=255)
                                                                                     cgggggggCGCCCCGCGGGGAGGGGGGGAAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTAAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAgg                 <  2:1578902/99‑1 (MQ=255)
                                                                                     ccGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAgg                 >  1:2262891/1‑101 (MQ=255)
                                                                                     ccGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAgg                 >  2:875047/1‑101 (MQ=255)
                                                                                       ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc               >  2:2460863/1‑101 (MQ=255)
                                                                                          gggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACg            <  1:2192265/101‑1 (MQ=255)
                                                                                             agccccGGGGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCGGGCGTGTTTGGGGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCg         <  2:959745/98‑1 (MQ=255)
                                                                                                 ccGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgt     >  1:1093052/1‑101 (MQ=255)
                                                                                                    gggggACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGGGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt  <  1:1027477/100‑1 (MQ=255)
                                                                                                    cgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt  >  2:2486782/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGG.CGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGT  >  NC_014034/3003986‑3004185

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG