BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,004,101	A→C	8.8%	intergenic (+147/+29)	gfo → / ← lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,004,101	0	A→C	8.8%	38.4 ‑0.8 ‑0.3	199	intergenic (+147/+29)	gfo/lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand): new base (10/8): ref base (119/62): total (129/70)
Fisher's exact test strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.19e-01

Only 100 of 225 total aligned reads displayed.


CACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCGGAT  >  NC_014034/3004001‑3004201


cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAC.ATCGCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGTCCCGCGGGGT.CCGGGGGTaga                                                                                                      >  2:1494995/1‑101 (MQ=25)


  ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGA.CGGGGGGCAGCgc                                                                                                    <  2:1107319/101‑1 (MQ=18)


  ccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgc                                                                                                    <  2:1936544/101‑1 (MQ=255)


     cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccc                                                                                                 <  2:416013/101‑1 (MQ=255)


      ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACGTTGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgccccc                                                                                                <  1:2039744/101‑1 (MQ=255)


      ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgccccc                                                                                                <  2:240473/101‑1 (MQ=255)


       cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGA.CGGGGGGCAGCgcccccc                                                                                               <  2:2360672/101‑1 (MQ=17)


       cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccc                                                                                               <  1:643344/101‑1 (MQ=255)


        gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccct                                                                                              <  1:723588/101‑1 (MQ=255)


         ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGA.CGGGGGGCAGCgccccccct                                                                                             >  2:3745149/1‑99 (MQ=18)


         ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGA.CGGGGGGCAGCgccccccct                                                                                             >  2:2280410/1‑99 (MQ=18)


         ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGA.CGGGGGGCAGCgccccccct                                                                                             >  2:394391/1‑99 (MQ=18)


         ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGA.CGGGGGGCAGCgccccccct                                                                                             <  1:3280179/101‑3 (MQ=17)


         ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccctg                                                                                             <  2:1751678/101‑1 (MQ=255)


          gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccctgc                                                                                            >  1:4164314/1‑101 (MQ=255)


           atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccctgcc                                                                                           >  1:399908/1‑101 (MQ=255)


            tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgccaccctgccc                                                                                          >  1:2215341/1‑101 (MQ=255)


             atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCCGAgcccccctgcccc                                                                                         >  2:1576894/1‑101 (MQ=255)


             atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccctgcccc                                                                                         >  2:1649620/1‑101 (MQ=255)


              tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccctgccccc                                                                                        >  2:1350770/1‑101 (MQ=255)


              tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccctgccccc                                                                                        >  2:4000102/1‑101 (MQ=255)


                tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAgcccccctgcccccct                                                                                      <  2:4351998/101‑1 (MQ=255)


                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                                   <  1:605704/101‑1 (MQ=255)


                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                                   >  1:4359095/1‑101 (MQ=255)


                     cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                                 >  2:3303478/1‑101 (MQ=255)


                     cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                                 >  1:3120553/1‑101 (MQ=255)


                      aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                                                                >  1:2125251/1‑101 (MQ=255)


                        ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                                                                              >  2:2568701/1‑101 (MQ=255)


                         gggAAAGCAG.ATCCGGGTTTGCCTTCCCCCTTGTGGAAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                                                                             <  1:3799953/99‑1 (MQ=21)


                          tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                                            <  2:326873/101‑1 (MQ=255)


                          tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                                            <  1:3372897/101‑1 (MQ=255)


                           gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                                                                           >  1:3745001/1‑101 (MQ=255)


                            aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                                                                          >  1:2741127/1‑101 (MQ=255)


                             aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGCGCGCt                                                                         >  1:353243/1‑101 (MQ=255)


                             aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                                                         <  1:43732/101‑1 (MQ=255)


                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                                      <  1:1453556/101‑1 (MQ=255)


                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                                      >  2:2193073/1‑101 (MQ=255)


                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                                      >  1:59041/1‑101 (MQ=255)


                                 aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                                                                     >  2:4368046/1‑101 (MQ=255)


                                  g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGc                                                                    <  1:2416094/101‑1 (MQ=255)


                                  g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                                                                    <  2:616431/101‑1 (MQ=255)


                                     tCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                                                                  <  2:854872/101‑1 (MQ=255)


                                     tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCACGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                                                                  >  2:3859693/1‑101 (MQ=255)


                                        cGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                                               <  2:4322873/101‑1 (MQ=255)


                                        cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                                               >  2:314446/1‑101 (MQ=255)


                                         ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt                                                              >  1:2304866/1‑101 (MQ=255)


                                          gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                                             <  2:3871861/101‑1 (MQ=255)


                                           tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                                            >  1:1061729/1‑101 (MQ=255)


                                            ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                                                           >  2:955019/1‑101 (MQ=255)


                                            ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                                                           >  1:2336690/1‑101 (MQ=255)


                                             tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                                                          >  1:2586525/1‑101 (MQ=255)


                                              gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa                                                         <  1:2945693/101‑1 (MQ=255)


                                               ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg                                                        <  2:2142160/101‑1 (MQ=255)


                                                cTTTAACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCATGTCGGGCGGGc                                                       >  2:909311/1‑101 (MQ=25)


                                                 tttCGCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                                      <  1:1223076/101‑1 (MQ=255)


                                                 tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGGCGAGCCCCCCTCCCCCCCCGGGGCCGAGCGCCCAGGGGTGGTTTGCGAggc                                                      >  1:945665/1‑99 (MQ=21)


                                                 tttCACCTTGGCGCAAATATCCCGGGGGGACTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGGGCGCTCAGGCGTGTTTGGGGAGcc                                                      >  2:4301326/1‑101 (MQ=255)


                                                  ttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg                                                     >  1:3933187/1‑101 (MQ=255)


                                                  ttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg                                                     <  2:3462235/101‑1 (MQ=255)


                                                      ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct                                                 >  1:3406820/1‑101 (MQ=255)


                                                       cTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt                                                >  2:1018497/1‑101 (MQ=255)


                                                        ttGGCGCAAATATCCGGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTc                                               <  1:4060964/101‑1 (MQ=255)


                                                            cgcAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                                           >  1:3245239/1‑101 (MQ=255)


                                                             gcAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCa                                          >  2:289395/1‑101 (MQ=255)


                                                                aaTATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                                       >  1:115809/1‑101 (MQ=255)


                                                                aaTATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                                       >  2:4246860/1‑101 (MQ=255)


                                                                aaTATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                                       >  1:3650333/1‑101 (MQ=255)


                                                                 atatCCGGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTc                                      <  2:2263007/101‑1 (MQ=255)


                                                                 atatCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGAGTTTGGCGAGCCGCTCTTCGAGCACGTc                                      >  1:1302637/1‑101 (MQ=255)


                                                                  tatCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCg                                     >  2:4398246/1‑101 (MQ=255)


                                                                    tCCCGGGGGGAGCCCGGGGGGGG.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGGGAGCCGCTCTTCGAGCACGTCGaa                                   <  2:479815/101‑1 (MQ=255)


                                                                        ggggggAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                               >  2:3076326/1‑101 (MQ=255)


                                                                        ggggggAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                               >  2:2460863/1‑101 (MQ=255)


                                                                        ggggggAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                               >  1:195538/1‑101 (MQ=255)


                                                                         gggggAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGcc                              >  2:1997411/1‑100 (MQ=255)


                                                                          ggggAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCAc                             >  1:3902250/1‑101 (MQ=255)


                                                                           gggAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACg                            <  1:2192265/101‑1 (MQ=255)


                                                                           gggAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACg                            <  1:3681633/101‑1 (MQ=255)


                                                                            gggcggccGCCGGGAGCGGGGGGCAGGCCCCCCCTCCCCCCTTGGGACGGAGCGCCGAGGTGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACg                            <  2:3698829/95‑1 (MQ=255)


                                                                             gAGTCCCGGGGGGG.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGcc                          <  1:2395719/101‑1 (MQ=255)


                                                                              agccccGGGGGGA.GGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCGGGCGTGTTTGGGGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCg                         <  2:959745/98‑1 (MQ=255)


                                                                                gccggcgGGGG.CGGGGGGAAGAGCCCCCCTGCCCCCGTGGGGCGGGGCGCTAGGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCggg                       <  1:1668278/97‑1 (MQ=255)


                                                                                 cccGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgggg                      >  2:1430028/1‑101 (MQ=255)


                                                                                  ccGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgt                     >  1:1093052/1‑101 (MQ=255)


                                                                                    gcgGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcg                   >  2:2655140/1‑101 (MQ=255)


                                                                                     gggggA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGGGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt                  <  1:1027477/100‑1 (MQ=255)


                                                                                     cgGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt                  >  2:2486782/1‑101 (MQ=255)


                                                                                       gggA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTcc                >  1:909445/1‑101 (MQ=255)


                                                                                       gggA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTcc                >  1:1280063/1‑101 (MQ=255)


                                                                                        ggA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCt               >  1:3024774/1‑101 (MQ=255)


                                                                                         gA.CGGGGGGCAGACCCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCtt              <  2:1640025/101‑1 (MQ=255)


                                                                                          a.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTg             >  1:4301013/1‑101 (MQ=255)


                                                                                            gggggggCAGAGCCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTgg            <  1:172249/100‑1 (MQ=255)


                                                                                               ggggCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGgcgc         >  2:2736794/1‑101 (MQ=255)


                                                                                                  gCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGc      >  1:2537625/1‑101 (MQ=255)


                                                                                                   cAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCg     >  2:2920331/1‑101 (MQ=255)


                                                                                                    agagCCCCCCTGCCGCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCgg    >  2:1250225/1‑101 (MQ=255)


                                                                                                    agagCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCgg    >  1:1761111/1‑101 (MQ=255)


                                                                                                    agagCCCCCCGGCCCCCCTGGGGCCGAGCGCTCAGGGGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCgg    <  1:343009/101‑1 (MQ=255)


                                                                                                     gagCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCGGa   >  2:754975/1‑101 (MQ=255)


CACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA.CGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCGGAT  >  NC_014034/3004001‑3004201

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG