Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,011,287 A→T 84.5% intergenic (+86/+56) purK → / ← RCAP_rcc02822 phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,011,2870A→T84.5% 87.2 ‑1.3 0.0174intergenic (+86/+56)purK/RCAP_rcc02822phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Reads supporting (aligned to +/- strand):  new base (91/56):  ref base (20/7):  total (111/63)
Fisher's exact test strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCC  >  NC_014034/3011194‑3011381
                                                                                               |                                                                                              
ttCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCgg                                                                                         <  2:538267/101‑1 (MQ=255)
ttCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCgg                                                                                         >  2:2177671/1‑101 (MQ=255)
ttCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCgg                                                                                         <  1:2835951/101‑1 (MQ=255)
 tctcTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCggg                                                                                        <  1:3362795/101‑1 (MQ=255)
   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCggggg                                                                                      <  2:328490/101‑1 (MQ=255)
   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCggggg                                                                                      >  1:1537853/1‑101 (MQ=255)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                     >  1:642079/1‑101 (MQ=255)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                     >  1:3101168/1‑101 (MQ=255)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATAGTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                     >  2:3851850/1‑101 (MQ=37)
      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgag                                                                                   >  1:3623660/1‑101 (MQ=255)
          gcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACtt                                                                               <  2:260373/101‑1 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTg                                                                              <  1:1617059/101‑1 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTg                                                                              <  1:1067168/101‑1 (MQ=255)
            ggCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGc                                                                             >  1:3187829/1‑101 (MQ=255)
              cAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCg                                                                           <  2:2506678/101‑1 (MQ=255)
                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAg                                                                        <  1:936548/101‑1 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                       >  2:2123230/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                       >  2:449619/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                       >  1:1870561/1‑101 (MQ=255)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                      >  2:2747044/1‑101 (MQ=255)
                    gggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTAACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCa                                                                     >  2:4407975/1‑101 (MQ=38)
                     ggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCaa                                                                    >  1:3767824/1‑101 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAag                                                                   >  1:255791/1‑101 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAag                                                                   >  2:3095165/1‑101 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAag                                                                   >  1:1583354/1‑101 (MQ=255)
                       cTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAaga                                                                  <  1:2570655/101‑1 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                                 >  2:708469/1‑101 (MQ=255)
                           cctcccccGCCCCCCCCAGGGTTATTCCGGCCAAGTGAAAACCACCGCCTTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAggg                                                               <  2:2862045/98‑1 (MQ=255)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggggg                                                            >  1:3461519/1‑101 (MQ=255)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggggg                                                            >  2:2290938/1‑101 (MQ=255)
                              cccgcGCACCCCCGAGGATATTTTCGCCAAGGTCAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                           <  2:2373084/101‑1 (MQ=37)
                              cccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                           <  1:88465/101‑1 (MQ=255)
                              cccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                           >  1:4242134/1‑101 (MQ=255)
                                cccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg                                                         <  2:4139141/101‑1 (MQ=255)
                                cccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg                                                         <  2:2967649/101‑1 (MQ=255)
                                    cACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcc                                                     >  2:2478883/1‑101 (MQ=255)
                                    cACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcc                                                     >  1:4195/1‑101 (MQ=255)
                                     aCCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGccc                                                    >  1:2543680/1‑101 (MQ=255)
                                     aCCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGccc                                                    >  1:499873/1‑101 (MQ=255)
                                      cccccGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcccc                                                   <  1:3665178/101‑1 (MQ=255)
                                        cccGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcccccc                                                 >  1:3283696/1‑101 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                              >  2:925262/1‑101 (MQ=255)
                                            aGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGcc                                             >  1:2792145/1‑101 (MQ=255)
                                              gATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCg                                           >  2:2805105/1‑101 (MQ=255)
                                               atatTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCgg                                          <  1:2220016/101‑1 (MQ=255)
                                                tatTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGc                                         >  1:3482206/1‑101 (MQ=255)
                                                tatTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGc                                         >  2:3043541/1‑101 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                        >  1:3608752/1‑101 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                        >  2:4270994/1‑101 (MQ=255)
                                                  tttCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTc                                       <  2:34113/101‑1 (MQ=255)
                                                  tttCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTc                                       <  1:2865267/101‑1 (MQ=255)
                                                  tttCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTc                                       <  2:3992131/101‑1 (MQ=255)
                                                   ttCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCa                                      >  1:1932355/1‑101 (MQ=255)
                                                   ttCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCa                                      <  2:372855/101‑1 (MQ=255)
                                                    tCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCATGCAATAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                     <  2:1446950/101‑1 (MQ=37)
                                                    tCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                     <  1:2157392/101‑1 (MQ=255)
                                                     ccGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACg                                    >  1:2101812/1‑101 (MQ=255)
                                                      cGCCGAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                   <  2:2021620/101‑1 (MQ=37)
                                                      cGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                   >  1:4056559/1‑101 (MQ=255)
                                                       gCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGc                                  >  1:3420150/1‑101 (MQ=255)
                                                         cAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATCTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                                <  2:1077590/101‑1 (MQ=255)
                                                         cAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                                >  1:1010802/1‑101 (MQ=255)
                                                         cAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                                >  2:1680077/1‑101 (MQ=255)
                                                          aaGGTGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                               >  2:1200863/1‑101 (MQ=255)
                                                          aaGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                               <  1:503007/101‑1 (MQ=255)
                                                           aGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAggg                              >  1:3931651/1‑101 (MQ=255)
                                                           aGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAggg                              >  2:1626653/1‑101 (MQ=255)
                                                             gTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                            >  2:786572/1‑101 (MQ=255)
                                                             gTGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                            <  2:1583248/101‑1 (MQ=255)
                                                              tGAAAA.CAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCa                           >  2:3874736/1‑101 (MQ=255)
                                                              tGAAAA.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCAGGCCCGGCTCACCGCAGGGCCa                           >  2:1589611/1‑101 (MQ=25)
                                                                 aaa.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAggggcggtc                        >  2:3796193/1‑95 (MQ=25)
                                                                 aaa.CAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                        >  2:4389118/1‑101 (MQ=255)
                                                                 aaa.CAAAGTCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                        >  2:143580/1‑101 (MQ=25)
                                                                  aa.caacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGGGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                       >  1:3835397/1‑101 (MQ=37)
                                                                  aa.caacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                       >  1:1454502/1‑101 (MQ=255)
                                                                  aa.caacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                       >  2:2297072/1‑101 (MQ=255)
                                                                   a.caacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                      >  2:2457055/1‑101 (MQ=255)
                                                                     ccccGCCCTTACCCCTGGGCAC.AATTAGCGCGGGGAGAGCCTTGCCCCAGGCAAGGGGGGGGCCGCGCCCCCCTGCGGGCCGCACGGCAGGGCCAGCCGCt                     <  2:2506885/98‑1 (MQ=255)
                                                                     caacGCCCTTCCCCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCGGCCCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCt                     <  1:2747234/101‑1 (MQ=38)
                                                                       acccccTTTCCCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGc                   <  2:1980220/98‑1 (MQ=25)
                                                                       acGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGc                   >  2:2542534/1‑101 (MQ=255)
                                                                        cGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCa                  >  2:577299/1‑101 (MQ=255)
                                                                        cGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCa                  >  2:3160359/1‑101 (MQ=255)
                                                                          cccTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAg                >  2:1493396/1‑101 (MQ=255)
                                                                           ccTTTCCCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAgg               <  2:2764153/101‑1 (MQ=37)
                                                                             tttCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGGCTCAAGGcc             >  2:3083622/1‑101 (MQ=25)
                                                                              ttCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcg            >  2:4439637/1‑101 (MQ=255)
                                                                               tCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgc           >  1:3721980/1‑101 (MQ=255)
                                                                               tCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgc           >  1:1922505/1‑101 (MQ=255)
                                                                               tCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgc           >  1:1504774/1‑101 (MQ=255)
                                                                                 aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCgcgc         >  2:3402938/1‑101 (MQ=255)
                                                                                  ccTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcc        >  1:2973579/1‑101 (MQ=255)
                                                                                   cTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCCGCCGCTGCAAGGCCGCGccc       >  1:4376899/1‑101 (MQ=37)
                                                                                    ttGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc      >  1:1916686/1‑101 (MQ=255)
                                                                                     tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa     >  2:580321/1‑101 (MQ=255)
                                                                                     tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa     >  2:18417/1‑101 (MQ=255)
                                                                                     tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa     >  1:2937364/1‑101 (MQ=255)
                                                                                      gggAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAg    <  1:4170000/101‑1 (MQ=255)
                                                                                        gAC.AAATGTCCCGGGGGGGGTCTCGCCCCAGGCGAGAGGGGGCCAGCGCCCCCCTGCCCGGCTCACGGCAGGCCCAGCCGCTGCAAGGCCGCGCCCCAGcc  <  2:3526127/101‑1 (MQ=37)
                                                                                               |                                                                                              
TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAA.CAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCC  >  NC_014034/3011194‑3011381

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG