Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,222 C→T 2.9% intergenic (‑205/+87) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,2220C→T 2.9% 2.7 ‑1.9 ‑0.8139intergenic (‑205/+87)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (56/79):  total (56/83)
Fisher's exact test strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.63e-01

Only 100 of 186 total aligned reads displayed.

CAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATCG  >  NC_014034/3068122‑3068322
                                                                                                         |                                                                                                      
cgggggCCTCGGGCCCGCCGGGGGCCC.AAGG.CC.CCCGGCCCAGCGCCCGCCCCGCCCAG.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                                                                                        <  1:3895399/97‑1 (MQ=11)
       cTCGTGACCGCCGAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCt                                                                                                 >  2:2155637/1‑101 (MQ=255)
        tCGTGACCGCCGAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCT.g                                                                                               >  1:4178452/1‑101 (MQ=255)
            gcccgccgGGGGCCCAAAGG.CC.CTCGG.CCGGCGCCCCCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGa                                                                                           <  1:2197436/99‑1 (MQ=255)
            gcccgccgAGGGCCCAAGGC.CC.CTGGG.CCGGCCCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGa                                                                                           <  2:2564448/99‑1 (MQ=17)
                gccgAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCC.GGGGGAACg                                                                                       >  2:2285164/1‑101 (MQ=255)
                ccgcggggCCCCAAGC.CC.CCCGC.CCGGCGCCCGCCCCGCCCAT.AGCGGGCGC.TTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGC.ggggcagac                                                                                       <  1:806305/96‑6 (MQ=14)
                 ccgAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACgg                                                                                      >  1:569544/1‑101 (MQ=255)
                 ccgAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCCCCCCGCCCAT.GGCGGGGGC.TTCTCGCCCGCCCGGGGGGGCCGCGGGCGCGGCTTCGCCttgagagcagg                                                                                       >  1:4245741/1‑91 (MQ=2)
                  cgggggCACAAAGC.CCACCCGG.CGCGCCCCCCCCCCGCCCCT.GGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGCCGGCCTCTGTTGTGCCTCGCGGAAACg                                                                                       <  1:789343/98‑1 (MQ=1)
                   gggggGCCAAAGGCCC.CCCGG.CCAGCGCCCGCCCCGCCCAG.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGt                                                                                     <  1:2756847/99‑1 (MQ=17)
                   ggggccccAAAGC.CC.CGCGC.CCCGCCCCCCCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTc                                                                                    <  1:94940/96‑1 (MQ=17)
                   gAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACgggc                                                                                    >  1:3082443/1‑99 (MQ=255)
                   gAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCCGGGCGGGGGCCGGCCTCTGCTGCGCCT.GGGGGAACgggc                                                                                    >  1:1714818/1‑99 (MQ=255)
                    ggggCCCAAAGGCCC.CCCGG.CAGGCCCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCCCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTc                                                                                    <  2:3693253/100‑1 (MQ=14)
                     ggtgccAAAGG.CC.CCGGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGTCGGGCGCTGGCCTCTGTTGCACTT.GGGGAAACGGTcgc                                                                                  <  2:2243335/97‑3 (MQ=14)
                     gggCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCCGGGCCC.GGGGGAACgggcgt                                                                                  >  1:1080923/1‑97 (MQ=14)
                      gccccAAGGG.CC.CGGGG.CCCGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTGCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTg                                                                                 <  2:1111415/99‑1 (MQ=17)
                         ccAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCCCTGCTGCGCCT.GGGGGAACGGGCCTgggc                                                                              >  2:1540988/1‑99 (MQ=14)
                          cAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCT.GGGGAAACGGGCTTGGCA.c                                                                            >  2:2058250/1‑101 (MQ=255)
                          cAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGCCCGGCCTCTGCTGCGCCC.GGGGAAACGGTCCTGGCA.c                                                                            >  2:4082969/1‑101 (MQ=17)
                           aaaGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCT.GGGGAAACGGGCTTTGCA.cc                                                                           >  2:1181234/1‑101 (MQ=255)
                            aacg.CC.CCCGG.CCCCCCCCCCCCCCCCCCGG.GGGGGGCCCTTTCCCCCCCGCGGGGGGGGCCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.cc                                                                           <  2:3476493/98‑1 (MQ=12)
                             aGG.CC.CCCCG.CCCGCCCCCGCCCCCCCCCTGGGTCGGTGC.TTCTCGCCCGCGGGGGCCGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCg                                                                          <  1:3806813/101‑1 (MQ=12)
                               gccc.cTCGG.CCAGCCCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGcc                                                                        <  1:2178935/99‑1 (MQ=255)
                               g.CC.GTCGC.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGccc                                                                       <  2:3367110/101‑1 (MQ=255)
                               g.CC.CTCGG.CCGGCCCCCGCCCCGCCCAT.GGCGGGCGC.TTCCCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGccc                                                                       <  1:1230525/101‑1 (MQ=255)
                               g.CC.CCCGC.CCGGCGCCCGCCCCGCCCAG.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGccc                                                                       <  1:2035821/101‑1 (MQ=18)
                                 gcccgggg.CCGGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGccc                                                                       <  1:415710/95‑1 (MQ=18)
                                 cc.cTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAAAGGTCTTTGGA.CCGCCCg                                                                      >  2:2573001/1‑101 (MQ=17)
                                    cTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCT.GGGGGAACCGGCTTTGCA.CCGCCCcgg                                                                    >  2:1025453/1‑101 (MQ=17)
                                    cTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCCGGGCGGGCGGCCGCCTCTGCTGCGCCT.GGGGGAACGGGCTTGGCACCCGCCCgg                                                                     >  2:3090597/1‑101 (MQ=17)
                                        gcccggcccccgccccgcCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTc                                                                 <  2:4321560/93‑1 (MQ=255)
                                        g.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTcc                                                                >  2:2675972/1‑101 (MQ=255)
                                          ccggcccccgccccgcCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCcg                                                               <  1:2059035/95‑1 (MQ=255)
                                           cAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCcgg                                                              >  2:3616461/1‑101 (MQ=255)
                                           cAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCcgg                                                              >  1:480110/1‑101 (MQ=255)
                                            aGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCcggc                                                             >  1:2602358/1‑101 (MQ=255)
                                               gcccgcccCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGGA.CCGCCCGGGCTCCggcggg                                                          >  2:2925692/1‑101 (MQ=255)
                                               cccccccccgcCCTG.GGCGGGCGC.TTCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCggcggg                                                          <  1:4083229/100‑1 (MQ=21)
                                                cccgccccgcCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCggcgggc                                                         >  1:2953170/1‑101 (MQ=255)
                                                 ccgccccgccCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGGCTTGGCA.CCGCCCGGGCTCCggcgggcg                                                        >  2:3262666/1‑101 (MQ=255)
                                                  cgccccgcccAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGGCTTGGCA.CCGCCCGGGGTCCGgcgggcgg                                                       >  2:3823363/1‑101 (MQ=255)
                                                    ccccggtctt.GGCGGGCGC.TCCCCGCCCGCCGGGGCGGTCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCgggg                                                     <  2:3935668/92‑1 (MQ=21)
                                                     cccgccgAG.GGCGGTCGC.TCCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGa                                                    <  2:907956/101‑1 (MQ=25)
                                                      ccgcccAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGaa                                                   >  2:490992/1‑101 (MQ=255)
                                                      ccgcccAG.GGGGGGCGC.TTCCCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGaa                                                   <  1:585543/101‑1 (MQ=255)
                                                       cgcccAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGgaa                                                  >  2:4142509/1‑101 (MQ=255)
                                                        gcccAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAg                                                 >  1:160693/1‑101 (MQ=255)
                                                          ccAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGGAAggg                                               >  1:4445019/1‑100 (MQ=255)
                                                          ccAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCCGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGGAAGGc                                               >  2:3054680/1‑101 (MQ=255)
                                                            cagggcgggcgC.TGCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCa                                              <  2:3348661/98‑1 (MQ=255)
                                                             t.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAgggcgg                                            >  2:240261/1‑97 (MQ=255)
                                                                gcgggcgC.TTCCCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGtac                                          <  2:569353/101‑3 (MQ=255)
                                                                 cgggcgC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGcc                                         >  1:1383882/1‑101 (MQ=255)
                                                                  gggcgC.TTCTCGCCGGCGGGGGCGGGCCCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCGGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCt                                        <  1:4027870/101‑1 (MQ=25)
                                                                   ggcgC.TTCTCGCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCtt                                       <  1:2411157/101‑1 (MQ=255)
                                                                    ggcgtctcccgcccgcgGGGGCGGGCCCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCtt                                       <  1:3148457/92‑1 (MQ=37)
                                                                      gc.TTCCCGCCCGCGGGGGGGGCCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTcc                                    <  1:4439927/101‑1 (MQ=37)
                                                                       c.TTCTCTCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCt                                   >  1:2745461/1‑101 (MQ=255)
                                                                       c.TTCTCGCCCGCCGGGGCGGGCCCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCt                                   <  2:1756929/101‑1 (MQ=255)
                                                                         ttggcccccgccGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTg                                  <  2:546642/95‑1 (MQ=255)
                                                                         ttCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTg                                  <  1:1300437/101‑1 (MQ=255)
                                                                         ttCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGGGCCTTTCCTg                                  >  2:279513/1‑101 (MQ=255)
                                                                          tctcGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCtaa                                 >  2:3526083/1‑99 (MQ=255)
                                                                           ctcGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGAt                                <  1:1660301/101‑1 (MQ=255)
                                                                           cccgcccgccGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGAt                                <  1:1434038/99‑1 (MQ=255)
                                                                            tcGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATc                               >  2:1926835/1‑101 (MQ=255)
                                                                             cgcccgccGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATcc                              >  2:2633487/1‑101 (MQ=255)
                                                                              ggcggggggggGGGGCGAAGGCCTCTGTCGTGCTT.GGGGAAACGGTCTTGGCA.CAGGTGATGAGCCAGCGGGCGGGGAAAGGCAGTGCCTTTCCCGATCCg                             <  1:2459313/94‑1 (MQ=25)
                                                                              gcccgccGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCg                             >  2:3966398/1‑101 (MQ=255)
                                                                                  gcgggggCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCg                         <  1:1850898/98‑1 (MQ=255)
                                                                                  gccgGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGGGCCTTTCCTGATCCGCCCg                         >  2:1270569/1‑101 (MQ=255)
                                                                                    gggggGGGCCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaa                       <  2:3027493/100‑1 (MQ=255)
                                                                                     ggggCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaaa                      >  2:2338181/1‑101 (MQ=255)
                                                                                     ggggCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaaa                      >  1:3343111/1‑101 (MQ=255)
                                                                                      gggcgggcGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAAt                     >  1:657879/1‑101 (MQ=255)
                                                                                      gggcgggcGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAAt                     <  1:2925913/101‑1 (MQ=255)
                                                                                       gggggacgcCGGCCTCTGCTGCGCCT.GGGGAAACGGTCGTGGCA.CCGCCCGGGCCCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATc                    <  1:1919993/95‑1 (MQ=25)
                                                                                          gggcgCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATcccc                 <  1:2519456/101‑1 (MQ=255)
                                                                                           ggcgCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATccccc                >  2:1032752/1‑101 (MQ=255)
                                                                                             cgcCGGACTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTc              >  2:63876/1‑101 (MQ=255)
                                                                                              gcCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCa             <  2:3672768/101‑1 (MQ=255)
                                                                                              cccGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCa             <  1:3118074/100‑1 (MQ=255)
                                                                                               ccGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAg            <  1:279548/101‑1 (MQ=255)
                                                                                               ccGGCCTCGGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGGGGGGAAAGCCAGTGCCTTTCTTGATCCGCCCGAAATCCCCCTCAg            <  2:4245472/101‑1 (MQ=37)
                                                                                                  gCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAgcgc         >  1:3418189/1‑101 (MQ=255)
                                                                                                   ccTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCa        <  2:1441450/101‑1 (MQ=255)
                                                                                                   ccTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCa        >  1:2851238/1‑101 (MQ=255)
                                                                                                    ctctGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAg       <  2:4444747/101‑1 (MQ=255)
                                                                                                    ctctGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCCCAGCGCAg       >  2:3834277/1‑101 (MQ=255)
                                                                                                      ctgctgCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCCGGa     >  2:1529859/1‑101 (MQ=255)
                                                                                                      ctgctgCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGa     <  1:4304574/101‑1 (MQ=255)
                                                                                                      ctgctgCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGa     <  1:1025541/101‑1 (MQ=255)
                                                                                                      ctgctgCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGa     >  1:1474728/1‑101 (MQ=255)
                                                                                                       tgctgcGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGAt    >  2:1232489/1‑101 (MQ=255)
                                                                                                        gctgcGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATc   >  2:4314048/1‑101 (MQ=255)
                                                                                                        gctgcGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATc   <  1:2566014/101‑1 (MQ=255)
                                                                                                        gctgcGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATc   >  2:1513719/1‑101 (MQ=255)
                                                                                                         ctgcGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATcg  <  1:491044/101‑1 (MQ=255)
                                                                                                         |                                                                                                      
CAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CC.CTCGG.CCAGCGCCCGCCCCGCCCAT.GGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCA.CCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATCG  >  NC_014034/3068122‑3068322

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG