Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 317,927 A→G 100% K338E (AAG→GAG)  RCAP_rcc00280 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034317,9270A→G100.0% 983.3 259K338E (AAG→GAG) RCAP_rcc00280hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (127/131):  ref base (1/0):  total (128/131)

Only 100 of 261 total aligned reads displayed.

AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024
                                                                                                  |                                                                                                 
 aGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAgg                                                                                                <  1:2689596/101‑1 (MQ=255)
   tGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAt                                                                                              >  2:3900881/1‑101 (MQ=255)
   tGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAt                                                                                              <  1:4113196/101‑1 (MQ=255)
    gACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtg                                                                                             >  1:1173189/1‑101 (MQ=255)
     aCGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtgt                                                                                            >  1:3385056/1‑101 (MQ=255)
         tGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctc                                                                                        <  2:2189146/101‑1 (MQ=255)
         tGGAGCCAGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGAAGCGGGAGATCTGCGCCCAGAGGATGTGctc                                                                                        >  2:3833705/1‑101 (MQ=255)
          ggAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctct                                                                                       >  2:2679453/1‑101 (MQ=255)
            aGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     <  2:4293332/101‑1 (MQ=255)
              cccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGAt                                                                                   <  1:2349739/101‑1 (MQ=255)
               ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  2:3810552/101‑1 (MQ=255)
               ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  1:2759160/101‑1 (MQ=255)
                ctcgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  1:1782265/99‑1 (MQ=255)
                cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  2:3979731/101‑1 (MQ=255)
                  cgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCaag                                                                               >  2:1782146/1‑99 (MQ=255)
                  cgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACg                                                                               <  2:3215792/101‑1 (MQ=255)
                   gacgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGc                                                                              >  1:2064017/1‑101 (MQ=255)
                     cgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGccc                                                                            <  1:943547/101‑1 (MQ=255)
                       acCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGa                                                                          <  2:1064696/101‑1 (MQ=255)
                       acCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGa                                                                          <  2:1423022/101‑1 (MQ=255)
                       acCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGa                                                                          <  2:2063880/101‑1 (MQ=255)
                         cGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAgg                                                                        >  1:3564677/1‑101 (MQ=255)
                          gAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGa                                                                       >  2:2098755/1‑101 (MQ=255)
                          gAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGa                                                                       >  1:2448832/1‑101 (MQ=255)
                           aGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      >  2:1507221/1‑101 (MQ=255)
                            gggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAt                                                                     >  1:3648084/1‑101 (MQ=255)
                             ggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAtt                                                                    >  2:533320/1‑101 (MQ=255)
                             ggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAtt                                                                    <  2:1241227/101‑1 (MQ=255)
                              gCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTc                                                                   <  2:66693/101‑1 (MQ=255)
                               cAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCg                                                                  <  1:1951568/101‑1 (MQ=255)
                                aaTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCgg                                                                 >  1:885192/1‑101 (MQ=255)
                                 aTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgc                                                                >  2:558416/1‑101 (MQ=255)
                                  tGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcg                                                               <  2:3135713/101‑1 (MQ=255)
                                  tGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcg                                                               >  2:2390578/1‑101 (MQ=255)
                                   gTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGAGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  1:721066/1‑101 (MQ=255)
                                     cTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCa                                                            <  2:1558143/101‑1 (MQ=255)
                                       ggATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATc                                                          >  1:2652030/1‑101 (MQ=255)
                                       ggATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATc                                                          <  2:2257636/101‑1 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         >  1:2980275/1‑101 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         <  1:828163/101‑1 (MQ=255)
                                         aTCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        <  2:1613645/101‑1 (MQ=255)
                                         aTCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        <  1:1857382/101‑1 (MQ=255)
                                         aTCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        <  2:328743/101‑1 (MQ=255)
                                          tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       >  1:3277869/1‑101 (MQ=255)
                                          tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGAGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       >  1:3102549/1‑101 (MQ=255)
                                           cACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAc                                                      <  1:1421753/101‑1 (MQ=255)
                                           cACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAc                                                      <  1:4324336/101‑1 (MQ=255)
                                             cGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCg                                                    >  2:1092902/1‑101 (MQ=255)
                                               cTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCggg                                                  <  2:2985894/101‑1 (MQ=255)
                                                tGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGc                                                 >  2:2909599/1‑101 (MQ=255)
                                                ggAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGc                                                 <  2:2682788/100‑1 (MQ=255)
                                                  aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               <  1:38558/101‑1 (MQ=255)
                                                  aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               <  1:3880928/101‑1 (MQ=255)
                                                    cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             >  2:781816/1‑101 (MQ=255)
                                                      gggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgggg                                           <  1:2789482/101‑1 (MQ=255)
                                                      gggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgggg                                           >  2:3356182/1‑101 (MQ=255)
                                                        gTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         <  1:3072400/101‑1 (MQ=255)
                                                        gTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         >  2:444723/1‑101 (MQ=255)
                                                            aTCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTccc                                     <  2:1829560/101‑1 (MQ=255)
                                                             tCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCt                                    <  2:1323098/101‑1 (MQ=255)
                                                              cTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCtt                                   <  1:2231470/101‑1 (MQ=255)
                                                               tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  2:3792149/101‑1 (MQ=255)
                                                                ggCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 <  1:1234277/101‑1 (MQ=255)
                                                                  ccGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcc                               >  1:1460603/1‑101 (MQ=255)
                                                                  ccGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcc                               >  2:3657298/1‑101 (MQ=255)
                                                                   cGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGccc                              >  2:2427890/1‑101 (MQ=255)
                                                                   cGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGccc                              >  1:1011376/1‑101 (MQ=255)
                                                                     aGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCg                            <  1:1650881/101‑1 (MQ=255)
                                                                      ggtggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAGCGGGCCTGGGGCTTCCCTTTGGCCCGc                           >  2:2004729/1‑101 (MQ=255)
                                                                       gtggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCt                          >  1:584112/1‑101 (MQ=255)
                                                                       gtggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCt                          <  2:4325273/101‑1 (MQ=255)
                                                                        tggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         >  2:2555956/1‑101 (MQ=255)
                                                                         ggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        <  2:4409894/101‑1 (MQ=255)
                                                                         ggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        <  1:3488862/101‑1 (MQ=255)
                                                                          gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtttt                       <  1:2825997/101‑1 (MQ=255)
                                                                            cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  2:1901584/1‑101 (MQ=255)
                                                                            cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     <  1:2539078/101‑1 (MQ=255)
                                                                            cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  1:2619029/1‑101 (MQ=255)
                                                                             gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  2:1607759/1‑101 (MQ=255)
                                                                              aTGCGGGCGATCTGCGCTCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTCACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGc                   >  2:4429156/1‑101 (MQ=255)
                                                                              aTGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGc                   <  1:2254768/101‑1 (MQ=255)
                                                                                gCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTa                 >  2:3698712/1‑101 (MQ=255)
                                                                                 cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                >  1:3200981/1‑101 (MQ=255)
                                                                                 cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                <  1:3779850/101‑1 (MQ=255)
                                                                                 cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                >  1:4234349/1‑101 (MQ=255)
                                                                                  gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               >  1:3166239/1‑101 (MQ=255)
                                                                                  gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               >  2:3036801/1‑101 (MQ=255)
                                                                                  gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTAGGAGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               >  2:2744673/1‑101 (MQ=255)
                                                                                   ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              <  2:3552347/101‑1 (MQ=255)
                                                                                    gCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcg             <  1:1349160/101‑1 (MQ=255)
                                                                                     cGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgc            >  1:3180867/1‑101 (MQ=255)
                                                                                      gATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgcc           >  2:3166594/1‑101 (MQ=255)
                                                                                      gATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgcc           >  1:661378/1‑101 (MQ=255)
                                                                                      gATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgcc           >  1:3136206/1‑101 (MQ=255)
                                                                                      gATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgcc           <  1:1113697/101‑1 (MQ=255)
                                                                                        tCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccgc         >  2:1513295/1‑101 (MQ=255)
                                                                                         cTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACgccgcc        >  1:3086310/1‑101 (MQ=255)
                                                                                            cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  2:288060/1‑101 (MQ=255)
                                                                                            cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     <  2:748896/101‑1 (MQ=255)
                                                                                            cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  2:867648/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG