BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,196,546	C→T	2.4%	A227T (GCC→ACC)	apbE ←	thiamine biosynthesis lipoprotein ApbE

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,196,546	0	C→T	2.4%	2.1 0.0 ‑0.0	164	A227T (GCC→ACC)	apbE	thiamine biosynthesis lipoprotein ApbE
Reads supporting (aligned to +/- strand): new base (2/2): ref base (75/84): total (78/86)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.70e-01

Only 100 of 206 total aligned reads displayed.


CCGCCGATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCTTTC  >  NC_014034/3196446‑3196646


ccgccgGTCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGtggcgatggc                                                                                                      <  1:1162324/101‑1 (MQ=255)


 cgccgATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGtggcgatggcg                                                                                                     <  1:2813543/101‑1 (MQ=255)


 cgccgATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGtggcgatggcg                                                                                                     >  1:4366389/1‑101 (MQ=255)


  gccgATCAGCGGCGCCCCGCGCTCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGtggcgatggcga                                                                                                    >  1:4147575/1‑101 (MQ=255)


  gccgATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGtggcgatggcga                                                                                                    <  1:1231408/101‑1 (MQ=255)


  gccgATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGtggcgatggcga                                                                                                    <  2:828973/101‑1 (MQ=255)


    cgATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGTgaga                                                                                                  >  1:3360535/1‑101 (MQ=255)


    cgATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCgaga                                                                                                  >  2:4406526/1‑101 (MQ=255)


    cgATCAGCGGCGCCCCGCGCGCGGGGGCCATCGTATGCGCCAGATGGCGGCCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCgaga                                                                                                  >  1:106342/1‑101 (MQ=255)


     gATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGAt                                                                                                 >  2:2301738/1‑101 (MQ=255)


       tCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGTGAGATCg                                                                                               >  1:1483407/1‑101 (MQ=255)


       tCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCg                                                                                               <  2:499868/101‑1 (MQ=255)


       tCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCg                                                                                               <  1:1911674/101‑1 (MQ=255)


       tCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCg                                                                                               <  2:1463166/101‑1 (MQ=255)


         aGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCCACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGt                                                                                             <  1:4389731/101‑1 (MQ=255)


          gcggcgCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTc                                                                                            <  1:3403186/101‑1 (MQ=255)


           cggcgCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCa                                                                                           <  2:767139/101‑1 (MQ=255)


              cgcCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGtt                                                                                        <  1:1381079/101‑1 (MQ=255)


               gcCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTc                                                                                       <  1:2518446/101‑1 (MQ=255)


               gatctgcgcgcgGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTc                                                                                       <  1:1116084/96‑1 (MQ=255)


                ccccGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCg                                                                                      >  1:421976/1‑101 (MQ=255)


                    gcgcgcgGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACa                                                                                  >  1:1314333/1‑101 (MQ=255)


                    gcgcgcgGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGCCAGTTcagatc                                                                                  >  2:1115993/1‑96 (MQ=255)


                       cgcgGGGTCCGTCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCg                                                                               >  1:4153275/1‑101 (MQ=255)


                       cgcgGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCg                                                                               <  1:2655539/101‑1 (MQ=255)


                         cgGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCa                                                                             <  2:1485334/101‑1 (MQ=255)


                          ggggTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCat                                                                            <  2:1172350/101‑1 (MQ=255)


                           gggTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCatg                                                                           <  1:856210/101‑1 (MQ=255)


                           gggTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCatg                                                                           >  2:3920457/1‑101 (MQ=255)


                           gggTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCatg                                                                           <  1:3278452/101‑1 (MQ=255)


                              tCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCatggat                                                                        <  1:2482938/101‑1 (MQ=255)


                              tCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCatggat                                                                        <  1:2301883/101‑1 (MQ=255)


                                cATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCatggatgg                                                                      <  1:285926/101‑1 (MQ=255)


                                cATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCatggatgg                                                                      >  2:2369074/1‑101 (MQ=255)


                                 aTCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGgc                                                                     <  1:3164993/101‑1 (MQ=255)


                                  tCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGgcg                                                                    <  1:4272176/101‑1 (MQ=255)


                                  tCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGgcg                                                                    <  1:764125/101‑1 (MQ=255)


                                       tGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATg                                                               >  2:3389914/1‑101 (MQ=255)


                                             aGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGaa                                                         >  2:1828108/1‑101 (MQ=255)


                                             aGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGaa                                                         <  2:1949747/101‑1 (MQ=255)


                                             aGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGaa                                                         >  1:3736449/1‑101 (MQ=255)


                                             aGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGaa                                                         <  2:3262292/101‑1 (MQ=255)


                                             aGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGaa                                                         >  2:3671362/1‑101 (MQ=255)


                                                tGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATc                                                      <  2:3168641/101‑1 (MQ=255)


                                                tGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATc                                                      <  1:4190229/101‑1 (MQ=255)


                                                    ggTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCgggg                                                  <  2:2836357/101‑1 (MQ=255)


                                                     gTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGc                                                 <  2:3079444/101‑1 (MQ=255)


                                                     gTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGc                                                 <  1:788758/101‑1 (MQ=255)


                                                       cGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGGTCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGt                                               >  2:2933920/1‑101 (MQ=255)


                                                       cGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGt                                               <  1:1334502/101‑1 (MQ=255)


                                                          cGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCa                                            <  2:3929925/101‑1 (MQ=255)


                                                           gACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCaa                                           <  2:3653682/101‑1 (MQ=255)


                                                           gACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCaa                                           >  1:599791/1‑101 (MQ=255)


                                                            aCCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAg                                          <  1:3691015/101‑1 (MQ=255)


                                                              ccGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAgcg                                        >  1:3032278/1‑101 (MQ=255)


                                                                 gACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCa                                     >  2:1510097/1‑101 (MQ=255)


                                                                  aCCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCAc                                    >  1:2361682/1‑101 (MQ=255)


                                                                   cccAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCAcc                                   <  1:2034847/101‑1 (MQ=255)


                                                                      aaTGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGcc                                >  1:1408645/1‑101 (MQ=255)


                                                                      aaTGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGcc                                >  1:3915406/1‑101 (MQ=255)


                                                                      aaTGACGGTAATCGCCCGAGGTGCCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGcc                                <  2:322112/101‑1 (MQ=255)


                                                                        tGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCa                              <  2:3961812/101‑1 (MQ=255)


                                                                        tGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCa                              <  1:1801760/101‑1 (MQ=255)


                                                                        tGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCa                              <  1:2018159/101‑1 (MQ=255)


                                                                        gatctgTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCa                              <  1:2258033/96‑1 (MQ=255)


                                                                         gCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAg                             >  2:1941498/1‑101 (MQ=255)


                                                                              tAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCcgcg                        >  1:1553240/1‑101 (MQ=255)


                                                                              tAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCcgcg                        >  2:1451497/1‑101 (MQ=255)


                                                                               aaTCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCgg                       >  1:3178533/1‑101 (MQ=255)


                                                                               aaTCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCgg                       >  2:1523740/1‑101 (MQ=255)


                                                                               aaTCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCgg                       >  2:1153173/1‑101 (MQ=255)


                                                                               aaTCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCgg                       >  1:1033517/1‑101 (MQ=255)


                                                                                   gCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGt                   >  1:997254/1‑101 (MQ=255)


                                                                                   gCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGGTCAAGCGCCACCGCCCAGCCGCGGTCGt                   >  1:2911219/1‑101 (MQ=255)


                                                                                     ccGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGttt                 >  2:3881287/1‑101 (MQ=255)


                                                                                     ccGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGttt                 >  2:4052430/1‑101 (MQ=255)


                                                                                      cGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGGCGTTTc                >  1:719053/1‑101 (MQ=255)


                                                                                       gAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTcc               <  2:4334938/101‑1 (MQ=255)


                                                                                       gAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTcc               <  2:4116994/101‑1 (MQ=255)


                                                                                        aGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCg              >  1:4351301/1‑101 (MQ=255)


                                                                                        aGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCg              <  1:2846370/101‑1 (MQ=255)


                                                                                         ggTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGt             >  1:3590902/1‑101 (MQ=255)


                                                                                            ggcgatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTccc          <  2:2754367/101‑1 (MQ=255)


                                                                                            ggcgatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTccc          >  2:2735777/1‑101 (MQ=255)


                                                                                            ggcgatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTccc          <  2:2232784/101‑1 (MQ=255)


                                                                                            ggcgatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTccc          >  2:2066786/1‑101 (MQ=255)


                                                                                             gcgatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCg         >  1:2006343/1‑101 (MQ=255)


                                                                                             gcgatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCg         <  1:2195768/101‑1 (MQ=255)


                                                                                               gatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCggc       >  2:720866/1‑100 (MQ=255)


                                                                                               gatggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGt       <  1:3928349/101‑1 (MQ=255)


                                                                                                atggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATTGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTc      <  2:4152000/101‑1 (MQ=255)


                                                                                                 tggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCt     <  2:3353392/101‑1 (MQ=255)


                                                                                                  ggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCtt    >  1:344269/1‑101 (MQ=255)


                                                                                                  ggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCtt    >  2:3489683/1‑101 (MQ=255)


                                                                                                  ggcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCtt    <  1:687968/101‑1 (MQ=255)


                                                                                                   gcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCttt   >  2:244738/1‑101 (MQ=255)


                                                                                                   gcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCttt   <  1:1510202/101‑1 (MQ=255)


                                                                                                   gcgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCAGGTCttt   <  1:3092287/101‑1 (MQ=255)


                                                                                                    cgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCTTTc  >  1:2136294/1‑101 (MQ=255)


                                                                                                    cgaGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCTTTc  <  2:64060/101‑1 (MQ=255)


CCGCCGATCAGCGGCGCCCCGCGCGCGGGGTCCATCGTATGCGCCAGATGGCGGTCGCCGACCCGGACCCAATGCCGGTAATCGCCCGAGGTGGCGATGGCGAGATCGGTCAGTTCGAACATCGCATGGATGGCGCGATGGCCGAAATCGGGGCGTTCAAGCGCCACCGCCCAGCCGCGGTCGTTTCCGTCCCGGTCTTTC  >  NC_014034/3196446‑3196646

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG