Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,694 A→C 100% intergenic (+19/‑164) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6940A→C100.0% 337.4 98intergenic (+19/‑164)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (6/89):  ref base (0/0):  total (9/89)

Only 100 of 120 total aligned reads displayed.

AGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACAGG  >  NC_014034/3254602‑3254788
                                                                                             |                                                                                               
ggaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGGGGGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.Tccc                                                                                       <  1:2111488/100‑1 (MQ=255)
agaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.Tccc                                                                                       <  1:2236816/101‑1 (MQ=255)
agaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.Tccc                                                                                       <  2:3942785/101‑1 (MQ=255)
  aCCGAAACGATCCTGATTCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.Tccccc                                                                                     <  2:4008605/101‑1 (MQ=255)
   ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGCCG.Cccccc                                                                                     >  2:3575043/1‑101 (MQ=255)
   ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCg                                                                                    <  1:1369231/101‑1 (MQ=255)
   ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCg                                                                                    <  2:2089359/101‑1 (MQ=255)
        aCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCAccc                                                                               <  2:3429703/101‑1 (MQ=255)
          gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCC.GCCCCCCCCccccc                                                                             >  2:2765814/1‑101 (MQ=255)
          gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCAccccc                                                                             <  2:3275694/101‑1 (MQ=255)
          gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGGGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGCGGCCG.CCCCCCCCCcccc                                                                              >  1:551990/1‑101 (MQ=255)
           aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGCGGGCGG.GCCCCCCCCccccc                                                                             >  1:63900/1‑101 (MQ=255)
           aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                            <  2:285780/101‑1 (MQ=255)
           aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                            <  1:2852981/101‑1 (MQ=255)
           aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                            <  1:207301/101‑1 (MQ=255)
           aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                            <  2:1941540/101‑1 (MQ=255)
           aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                            <  2:470317/101‑1 (MQ=255)
           aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                            <  2:756433/101‑1 (MQ=255)
            tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTg                                                                           <  1:781500/101‑1 (MQ=255)
             ccTGATGCGCTCGACAACCGGCTCGGTGCGCCGTGTGCTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCCCCCCCTgc                                                                          <  2:2965548/101‑1 (MQ=255)
                gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTgttcg                                                                       <  2:3053207/101‑5 (MQ=255)
                gATGCGCTCGAAAACCGGCTCGGTGCACCGTGTGATCTATCGCACCCCGATCCGCTGAGGCACCGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGcgcg                                                                       <  2:3388837/101‑1 (MQ=255)
                 aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCGGGGGGGGGGCCG.CCCCCCCCCccccccccgc                                                                        >  1:1714196/1‑96 (MQ=255)
                 aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGTGGCCG.GCCCCCCCCcccccccccgc                                                                       >  1:1487489/1‑95 (MQ=255)
                   gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTAt                                                                    <  2:2077103/101‑1 (MQ=255)
                   gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTAt                                                                    <  2:1628371/101‑1 (MQ=255)
                    cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTAtt                                                                   <  2:1805234/101‑1 (MQ=255)
                       tCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTg                                                                <  1:1618326/101‑1 (MQ=255)
                           aaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCaa                                                            <  2:2089945/101‑1 (MQ=255)
                            aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCGC.GCCCCCCCGCCCCCCGCGCGCAttttttccag                                                           >  2:470286/1‑95 (MQ=255)
                            aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAg                                                           <  1:333216/101‑1 (MQ=255)
                             aCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAgg                                                          <  2:3045421/101‑1 (MQ=39)
                             aCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAgg                                                          <  1:30227/101‑1 (MQ=255)
                               cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaa                                                        <  2:868916/101‑1 (MQ=38)
                               cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaa                                                        <  1:3917274/101‑1 (MQ=38)
                                ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                                       <  2:3573675/101‑1 (MQ=38)
                                ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                                       <  1:2750979/101‑1 (MQ=38)
                                 gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                                      <  1:1677979/101‑1 (MQ=38)
                                  cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAAc                                                     <  2:3258352/101‑1 (MQ=38)
                                    cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCCG..CCCCCCCCCCCCCCCCCCGCATTTTGCCCAGCAGAAcag                                                   >  2:1003778/1‑99 (MQ=255)
                                    cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACgg                                                   <  1:3682548/101‑1 (MQ=38)
                                      gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCTGGCCGCCCCCCCCCCCCCCCCGCGGGT.TTTTCCAAAGCAAAACggg                                                  >  2:4134192/1‑101 (MQ=255)
                                      gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGCCG.CCCCCCCCCCCCCCCCCGCGCTTTTTTCCAAGGAAAACggg                                                  >  1:1271062/1‑101 (MQ=255)
                                       tGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGc                                                <  2:1972154/101‑1 (MQ=38)
                                        gcgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGcc                                               <  1:3216290/101‑1 (MQ=38)
                                           ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGtt                                            <  2:2819903/101‑1 (MQ=38)
                                            cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGttt                                           <  2:3548507/101‑1 (MQ=38)
                                            cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGttt                                           <  2:780412/101‑1 (MQ=38)
                                              ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCa                                         <  2:1162709/101‑1 (MQ=38)
                                                tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CCGCCG.GCCCCCGCACCCCCCGCGCGTATTTTGCCACAGAAAACAGGGGCGGTTCAcc                                       >  2:547540/1‑101 (MQ=255)
                                                tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCAcc                                       <  2:1273527/101‑1 (MQ=38)
                                                 gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCt                                      <  1:1389605/101‑1 (MQ=38)
                                                 gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCt                                      <  2:1097692/101‑1 (MQ=38)
                                                  aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                                     <  2:1502511/101‑1 (MQ=38)
                                                  aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                                     <  1:3572761/101‑1 (MQ=38)
                                                   tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                                    <  2:3633531/101‑1 (MQ=38)
                                                   tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                                    <  2:2026918/101‑1 (MQ=38)
                                                     tATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGGCG.TCCCCCGCACCCCCCCCGCGGATTTTTCCCAGGAAAAAGGGGGCGGTTCACCTTGGc                                  >  2:2421992/1‑101 (MQ=16)
                                                     tATCGAAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCA.CACG.CCCCCCCCCCCCCCTGCCCCGGTTTTGCCCACGAAAAAAGGGGCGGTTCAACTTTGc                                  >  2:736775/1‑101 (MQ=255)
                                                      aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCG.CCCCCCCCCCCCCCCCCCCGTATTTTTCCCAAGAAAAAGGGGCCGCTTTAcccctgcc                                 >  1:781342/1‑95 (MQ=255)
                                                      aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CCGCCC.TCCCCCGCCCCCCCCGCGCGGATTTTGCCAAAGAAAAAGGGGGCGTTTCACCTTgggc                                 >  2:2167348/1‑99 (MQ=255)
                                                       tCGCAACCCGCTCCGGTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  2:1829969/101‑1 (MQ=37)
                                                       tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  2:3718844/101‑1 (MQ=38)
                                                       tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  1:1852414/101‑1 (MQ=38)
                                                       tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  1:3327262/101‑1 (MQ=38)
                                                       ccGCGACCCGAGCCGCTGAGGCAACGCT.GC.GGGGGACTGCCC.TCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  1:3006345/100‑1 (MQ=37)
                                                        cGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCCG.CCCCCCCCCCCCCCCCCCCCCCTTTTTCTAAGAAAAAAGGGG.CGGTTCACCCTTGCCgc                               >  2:1708463/1‑100 (MQ=255)
                                                        cGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGa                               <  2:3599946/101‑1 (MQ=38)
                                                          cAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGCGGCCG.CCCCCCCCCCCCCCCCCCCGCTTTTTTCCACGAAAAAAGGGGCGGGTTTTCCTTGGCCGaa                              >  2:2482409/1‑101 (MQ=255)
                                                           accccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                            <  1:892508/99‑1 (MQ=38)
                                                            aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAata                           <  2:416097/101‑1 (MQ=38)
                                                             cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAatat                          <  2:1917931/101‑1 (MQ=38)
                                                              ccGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCC.GCCCCCCCCCCCCCCCCGCGGATTTTTCCCAGGAAAAAAGGGGCGGTTTACCCTTGCCGcaaaaac                         >  2:3968214/1‑97 (MQ=255)
                                                                gATCCGCTGAGGCAACGCT.ACGGGGGGCA.CCCG.TCCCCCCCCACCCCCGCCCGTATTTTTTCAAAGAAAAAGGGGCCGTTTCACCTTTGCCCCGATATccc                       >  2:636659/1‑101 (MQ=255)
                                                                gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATccc                       <  1:2216929/101‑1 (MQ=38)
                                                                  tCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgg                     <  1:2646192/101‑1 (MQ=38)
                                                                  tCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgg                     <  2:2798624/101‑1 (MQ=38)
                                                                   ccGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCggg                    <  2:2744704/101‑1 (MQ=38)
                                                                    cGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgggg                   <  2:4145510/101‑1 (MQ=38)
                                                                     gCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCggggg                  <  1:2078631/101‑1 (MQ=38)
                                                                      cTGAGGCAACGCT.AC.GGGGGACTGCCG.GCCCCCGCCCCCCCCGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGGCGGAAAATCCCgggggg                 >  2:2969081/1‑101 (MQ=25)
                                                                        gAGGCAACGCT.ACGGGGGG.CTGCCG.TCCCCCCCCCCCCCCGCGCGTATTTTGCCCAGGAAAACGGGGCCGGTTCACCTTGGCCGAAAAATCCCcggggggg               >  2:3712864/1‑100 (MQ=255)
                                                                        gAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGa               <  1:470338/101‑1 (MQ=38)
                                                                        gAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGa               <  2:3481536/101‑1 (MQ=38)
                                                                         aGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGaa              <  1:2420814/101‑1 (MQ=38)
                                                                          ggCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAt             <  2:1695458/101‑1 (MQ=38)
                                                                          ggCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAt             <  2:3635245/101‑1 (MQ=38)
                                                                           gCAACGCT.ACGGGGGG.CTGCCG.TCCCCCGCCCCCCCCGCGCGGATTTTGCCAAGGAAAAAGGGGCCGTTTCACCTTGGCCGAAATATCCCggggggggagt            >  2:4046126/1‑97 (MQ=25)
                                                                            cAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt           <  1:17647/101‑1 (MQ=38)
                                                                            cAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt           <  1:2972945/101‑1 (MQ=38)
                                                                                acgctacgggggACTGCCG.TCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGc         <  1:919561/94‑1 (MQ=38)
                                                                                acgctacgggggACTGCCC.TCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGc         <  1:4383657/94‑1 (MQ=38)
                                                                                  gctacgggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCg       <  2:1520245/96‑1 (MQ=255)
                                                                                   ctacgggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGa      <  1:2302175/97‑1 (MQ=255)
                                                                                    tacgggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGAc     <  1:2098049/98‑1 (MQ=255)
                                                                                    tacgggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGAc     <  2:2907415/98‑1 (MQ=255)
                                                                                    tacgggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGAc     <  1:1350671/98‑1 (MQ=255)
                                                                                      cgggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACAg   <  1:4055917/100‑1 (MQ=255)
                                                                                      cgggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACAg   <  1:766141/100‑1 (MQ=255)
                                                                                       gggggACTGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGGATTTGCCGACAgg  >  1:2096774/1‑101 (MQ=255)
                                                                                             |                                                                                               
AGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACAGG  >  NC_014034/3254602‑3254788

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG