Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,190 A→C 6.1% K445Q (AAG→CCG)  recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1900A→C 6.1% 12.1 ‑2.1 ‑1.9115K445Q (AAG→CAG) recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (1/6):  ref base (51/57):  total (52/63)
Fisher's exact test strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.21e-02

Only 100 of 116 total aligned reads displayed.

GCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256109‑3256278
                                                                                      |                                                                                                
gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.tt                                                                                 >  2:3900584/1‑100 (MQ=255)
  cGAGGGGCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttgc                                                                               <  2:1518544/101‑4 (MQ=255)
  cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgc                                                                               >  2:1242136/1‑98 (MQ=255)
    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                                             >  1:943869/1‑96 (MQ=255)
    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                                             >  1:3535347/1‑96 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                                            >  1:3123774/1‑95 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                                            <  2:1219197/101‑7 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                                            <  1:4062803/101‑7 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                                            >  2:2226666/1‑95 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcc                                                                            >  1:3422125/1‑95 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttgctcg                                                                            <  2:1740750/101‑7 (MQ=255)
      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttgctcgc                                                                           <  2:1539701/101‑8 (MQ=255)
       ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgcc                                                                          <  1:2586236/101‑9 (MQ=255)
       ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttgctcgcc                                                                          <  2:2228428/101‑9 (MQ=255)
         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgcnngnccn                                                                        >  1:1520125/1‑91 (MQ=255)
           tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                       <  2:3632108/101‑1 (MQ=255)
           tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                       <  1:1802939/101‑1 (MQ=255)
           tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGc                                                                       <  1:4197041/101‑1 (MQ=255)
            ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                      <  1:665718/101‑1 (MQ=255)
            ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                      <  2:59330/101‑1 (MQ=255)
               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC.ggg                                                                  >  1:1914198/1‑101 (MQ=255)
               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.ggg                                                                  >  2:2296240/1‑101 (MQ=255)
               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.ggg                                                                  <  2:2585952/101‑1 (MQ=255)
               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.ggg                                                                  >  1:3952799/1‑101 (MQ=255)
                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgc                                                                 >  2:1037877/1‑101 (MQ=255)
                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgc                                                                 <  1:4161350/101‑1 (MQ=255)
                 cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcg                                                                <  1:2068416/101‑1 (MQ=255)
                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgggc                                                               >  1:1352026/1‑101 (MQ=255)
                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcgc                                                               <  2:3821496/101‑1 (MQ=255)
                    gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGC.gg                                                            >  2:495393/1‑101 (MQ=255)
                    gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gg                                                            <  2:1431411/101‑1 (MQ=255)
                    gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gg                                                            <  2:2815073/101‑1 (MQ=255)
                      gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                          <  2:2356144/101‑3 (MQ=255)
                      gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                          <  1:1029373/101‑3 (MQ=255)
                      gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                          <  1:283067/101‑3 (MQ=255)
                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggcac                                                        >  1:3908448/1‑97 (MQ=255)
                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggcac                                                        <  1:701771/101‑5 (MQ=255)
                          cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gg                                                      >  1:3470692/1‑101 (MQ=255)
                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.ggg                                                     >  2:2568168/1‑100 (MQ=255)
                            ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggc                                                    <  2:2548104/101‑3 (MQ=255)
                              cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                                  <  2:494783/101‑5 (MQ=255)
                              cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                                  <  1:558534/101‑5 (MQ=255)
                              cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                                  <  1:4253680/101‑5 (MQ=255)
                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcggc                                                 >  2:1469339/1‑96 (MQ=255)
                                ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCACGGGcg.gcc                                                >  2:3599619/1‑101 (MQ=255)
                                ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcc                                                <  2:3563113/101‑1 (MQ=255)
                                ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcc                                                <  1:1834940/101‑1 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccg                                              <  1:1031322/101‑1 (MQ=255)
                                   cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccgg                                             >  1:699642/1‑101 (MQ=255)
                                    aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccggc                                            <  1:1403647/101‑1 (MQ=255)
                                     tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGG.GTTGCTCGTCCGGCAGGGGCCC.GGGACGGGcg.gcccggcc                                           >  2:513802/1‑101 (MQ=255)
                                     tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCC.GGGCGCGGGCACGGGcg.gccccgcc                                           >  1:3474846/1‑101 (MQ=255)
                                     tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccggcc                                           <  2:1100231/101‑1 (MQ=255)
                                      ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCg                                          <  1:171620/101‑1 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                        <  1:900555/101‑1 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                        <  2:1438779/101‑1 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                        <  1:3446593/101‑1 (MQ=255)
                                         cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATc                                       <  2:1958504/101‑1 (MQ=255)
                                            gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCTATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGGTCTGc                                    >  1:3011321/1‑101 (MQ=255)
                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCg                                   >  1:4098301/1‑101 (MQ=255)
                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCCCGGGCG.GGCCGGCCGCTCTGCg                                   >  2:2074016/1‑101 (MQ=255)
                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCg                                   <  2:554941/101‑1 (MQ=255)
                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCGGcc                                >  1:3569720/1‑100 (MQ=255)
                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCt                                >  1:663391/1‑101 (MQ=255)
                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCt                                >  2:2560430/1‑101 (MQ=255)
                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAC.GGGGCGCCCCGCCGATCttgggcc                                >  1:349589/1‑95 (MQ=255)
                                                 tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCCGGCGGTCTGCGGCTg                               >  2:3261672/1‑101 (MQ=255)
                                                  gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTgg                              <  1:663097/101‑1 (MQ=255)
                                                     cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACg                           <  2:4114351/97‑1 (MQ=255)
                                                     cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCCGCCGCTCTGCGGCTGGagg                           >  2:2544728/5‑99 (MQ=255)
                                                      ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACgg                          <  1:3901193/98‑1 (MQ=255)
                                                       tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGCTCTGCGGCTGGGCggg                         >  1:1833705/3‑101 (MQ=255)
                                                       tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCCGGGCggg                         >  1:3211977/3‑101 (MQ=255)
                                                         gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCt                       <  2:3789580/101‑1 (MQ=255)
                                                             agttcaagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGGTCTGCGGCTGGGCCGGGTCCTg                   >  2:292580/10‑101 (MQ=255)
                                                              ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:1469444/93‑1 (MQ=255)
                                                              ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:254437/93‑1 (MQ=255)
                                                              ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:2047568/93‑1 (MQ=255)
                                                                cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  2:3908194/95‑1 (MQ=255)
                                                                 gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc               >  1:3622534/6‑101 (MQ=255)
                                                                 gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc               <  2:4160713/96‑1 (MQ=255)
                                                                 gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc               <  2:3474606/96‑1 (MQ=255)
                                                                  cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGcc              >  2:2762653/5‑101 (MQ=255)
                                                                  cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGcc              >  1:2369759/5‑101 (MQ=255)
                                                                  cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  1:1962583/97‑1 (MQ=255)
                                                                    gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGCCgg            >  1:2372415/3‑101 (MQ=255)
                                                                     ctgtaccccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCAATCTGCGGCTGGACGGGCTCCTGATGCCggg           <  1:1647977/94‑1 (MQ=255)
                                                                     ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg           <  2:3915863/100‑1 (MQ=255)
                                                                      tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCgggg          >  1:946604/1‑100 (MQ=255)
                                                                      tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCCCCCGATGCCgggg          >  1:1750420/1‑100 (MQ=255)
                                                                         aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  1:2744790/98‑1 (MQ=255)
                                                                         aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  2:2137209/98‑1 (MQ=255)
                                                                         aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  2:1823918/98‑1 (MQ=255)
                                                                         aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCCGGGCGGGCTCCTGATGCCGGGcgcc       >  2:2089296/4‑101 (MQ=255)
                                                                         aacccGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGcccc       >  2:2892527/4‑101 (MQ=255)
                                                                          acccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg      <  2:1359245/99‑1 (MQ=255)
                                                                            ccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg    <  2:3313452/101‑1 (MQ=255)
                                                                             cgcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGCTCTGCGGCTGGGCGGGCTCCCGGTGCCGGGCGCcgcg  >  1:1755174/1‑100 (MQ=255)
                                                                              gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCCGATGCCGGGCGCcgcggc  >  1:4151829/1‑99 (MQ=255)
                                                                              gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:3273288/101‑1 (MQ=255)
                                                                                      |                                                                                                
GCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256109‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG