Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,204 T→C 100% C449C (TGT→TGC recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2040T→C100.0% 400.0 107C449C (TGT→TGCrecJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (46/61):  ref base (0/0):  total (46/61)

Only 100 of 112 total aligned reads displayed.

TTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCA.C.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCG  >  NC_014034/3256119‑3256292
                                                                                              |                                                                                             
ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                                                                       >  1:4297851/1‑101 (MQ=255)
 tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                                      <  2:3632108/101‑1 (MQ=255)
 tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                                      <  1:1802939/101‑1 (MQ=255)
  ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                                     <  2:59330/101‑1 (MQ=255)
  ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                                     <  1:3133041/101‑1 (MQ=255)
  ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                                     <  1:665718/101‑1 (MQ=255)
    gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.gg                                                                                  >  2:1671341/1‑101 (MQ=255)
     cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC.ggg                                                                                 >  1:1914198/1‑101 (MQ=255)
     cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.ggg                                                                                 >  1:3952799/1‑101 (MQ=255)
     cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.ggg                                                                                 <  2:2585952/101‑1 (MQ=255)
     cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.ggg                                                                                 >  2:2296240/1‑101 (MQ=255)
      tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgc                                                                                <  1:4161350/101‑1 (MQ=255)
      tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgc                                                                                >  2:1037877/1‑101 (MQ=255)
       cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcg                                                                               <  1:2068416/101‑1 (MQ=255)
        aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgggc                                                                              >  1:1352026/1‑101 (MQ=255)
        aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcgc                                                                              <  1:4013088/101‑1 (MQ=255)
        aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcgc                                                                              <  2:3821496/101‑1 (MQ=255)
          gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGC.gg                                                                           >  2:495393/1‑101 (MQ=255)
          gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gg                                                                           <  2:1431411/101‑1 (MQ=255)
          gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gg                                                                           <  2:2815073/101‑1 (MQ=255)
            gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                                         <  2:2356144/101‑3 (MQ=255)
            gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                                         <  1:283067/101‑3 (MQ=255)
            gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                                         <  1:1029373/101‑3 (MQ=255)
              ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggcac                                                                       <  1:701771/101‑5 (MQ=255)
              ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggcac                                                                       >  1:3908448/1‑97 (MQ=255)
                cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gg                                                                    >  1:3470692/1‑101 (MQ=255)
                 gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.ggg                                                                   >  2:2568168/1‑100 (MQ=255)
                  ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggc                                                                  <  2:2548104/101‑3 (MQ=255)
                    cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                <  1:558534/101‑5 (MQ=255)
                    cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                <  1:4253680/101‑5 (MQ=255)
                    cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                <  2:494783/101‑5 (MQ=255)
                     aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcggc                                                               >  2:1469339/1‑96 (MQ=255)
                      ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGcg.gcc                                                              >  2:3599619/1‑101 (MQ=255)
                      ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcc                                                              <  2:3563113/101‑1 (MQ=255)
                      ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcc                                                              <  1:1834940/101‑1 (MQ=255)
                       cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gccc                                                             <  2:1334541/101‑1 (MQ=255)
                        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcggtgta                                                            <  2:4149542/101‑9 (MQ=255)
                        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccg                                                            <  2:3148867/101‑1 (MQ=255)
                        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccg                                                            <  1:1031322/101‑1 (MQ=255)
                         cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccgg                                                           >  1:699642/1‑101 (MQ=255)
                          aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccggc                                                          <  1:1403647/101‑1 (MQ=255)
                           tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGG.GTTGCTCGTCCGGCAGGGGCCC.GGGA.CGGGcg.gcccggcc                                                         >  2:513802/1‑101 (MQ=255)
                           tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCC.GGGCGCGGGCA.CGGGcg.gccccgcc                                                         >  1:3474846/1‑101 (MQ=255)
                           tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccggcc                                                         <  2:1100231/101‑1 (MQ=255)
                            ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCg                                                        <  1:171620/101‑1 (MQ=255)
                              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                      <  1:900555/101‑1 (MQ=255)
                              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                      <  1:3446593/101‑1 (MQ=255)
                               cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATc                                                     <  2:1958504/101‑1 (MQ=255)
                                  gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCTATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGGTCTGc                                                  >  1:3011321/1‑101 (MQ=255)
                                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGCG.GCCCGGCCGATCTGCg                                                 >  1:4098301/1‑101 (MQ=255)
                                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCC.CGGGCG.GGCCGGCCGCTCTGCg                                                 >  2:2074016/1‑101 (MQ=255)
                                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCg                                                 <  2:554941/101‑1 (MQ=255)
                                      cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCt                                              >  1:663391/1‑101 (MQ=255)
                                      cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCt                                              >  2:2560430/1‑101 (MQ=255)
                                      cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.C.GGGGCGCCCCGCCGATCttgggcc                                              >  1:349589/1‑95 (MQ=255)
                                       tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCCGGCGGTCTGCGGCTg                                             >  2:3261672/1‑101 (MQ=255)
                                       tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTTCGGccg                                             >  1:1872995/1‑99 (MQ=255)
                                        gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTgg                                            <  1:663097/101‑1 (MQ=255)
                                           cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACg                                         <  2:4114351/97‑1 (MQ=255)
                                           cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCCGCCGCTCTGCGGCTGGagg                                         >  2:2544728/5‑99 (MQ=255)
                                            ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACgg                                        <  1:3901193/98‑1 (MQ=255)
                                            ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCCGCCGATCTGCGGCTgggggg                                        >  1:3528768/4‑97 (MQ=255)
                                             tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGCTCTGCGGCTGGGCggg                                       >  1:1833705/3‑101 (MQ=255)
                                             tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCCGGGCggg                                       >  1:3211977/3‑101 (MQ=255)
                                               gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCt                                     <  2:3789580/101‑1 (MQ=255)
                                                   agttcaagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGGTCTGCGGCTGGGCCGGGTCCTg                                 >  2:292580/10‑101 (MQ=255)
                                                    ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                <  1:1469444/93‑1 (MQ=255)
                                                    ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                <  1:254437/93‑1 (MQ=255)
                                                    ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                <  1:2047568/93‑1 (MQ=255)
                                                      cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                              <  2:3908194/95‑1 (MQ=255)
                                                       gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc                             >  1:3622534/6‑101 (MQ=255)
                                                       gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                             <  2:4160713/96‑1 (MQ=255)
                                                        cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGTTGcc                            >  2:215707/5‑101 (MQ=255)
                                                        cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGcc                            >  2:2762653/5‑101 (MQ=255)
                                                        cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGcc                            >  1:2369759/5‑101 (MQ=255)
                                                          gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGCCgg                          >  1:2372415/3‑101 (MQ=255)
                                                          gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg                          <  2:2548462/99‑1 (MQ=255)
                                                           ctgtaccccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCAATCTGCGGCTGGACGGGCTCCTGATGCCggg                         <  1:1647977/94‑1 (MQ=255)
                                                            tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCgggg                        >  1:946604/1‑100 (MQ=255)
                                                            tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCCCCCGATGCCgggg                        >  1:1750420/1‑100 (MQ=255)
                                                               aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                     <  1:2744790/98‑1 (MQ=255)
                                                               aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                     <  2:2137209/98‑1 (MQ=255)
                                                               aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                     <  2:1823918/98‑1 (MQ=255)
                                                               aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCCGGGCGGGCTCCTGATGCCGGGcgcc                     >  2:2089296/4‑101 (MQ=255)
                                                               aacccGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGcccc                     >  2:2892527/4‑101 (MQ=255)
                                                                acccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                    <  2:1359245/99‑1 (MQ=255)
                                                                  ccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                  <  2:3313452/101‑1 (MQ=255)
                                                                   cgcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGCTCTGCGGCTGGGCGGGCTCCCGGTGCCGGGCGCcgcgc                 >  1:1755174/1‑100 (MQ=255)
                                                                    gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCCGATGCCGGGCGCcgcggc                >  1:4151829/1‑99 (MQ=255)
                                                                    gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGGGag                >  2:3691921/1‑100 (MQ=255)
                                                                    gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                <  1:3273288/101‑1 (MQ=255)
                                                                          ggcacggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCATG.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCTCGACGCCcg           <  2:1073871/91‑1 (MQ=255)
                                                                            cacggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgcg         <  1:2226811/93‑1 (MQ=255)
                                                                              cggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt       <  2:3123550/95‑1 (MQ=255)
                                                                               ggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg      <  1:1242229/96‑1 (MQ=255)
                                                                                  tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc   >  1:126989/3‑101 (MQ=255)
                                                                                  tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc   <  2:3023774/99‑1 (MQ=255)
                                                                                  tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGACGCCGCGACGCCCGCGCTGATc   <  2:1323500/99‑1 (MQ=255)
                                                                                   ggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  >  1:1326670/2‑101 (MQ=255)
                                                                                   ggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  >  2:389409/2‑101 (MQ=255)
                                                                                              |                                                                                             
TTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCA.C.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCG  >  NC_014034/3256119‑3256292

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG