Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,333,494	C→G	100%	intergenic (+40/‑126)	ccmH → / → paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,333,494	0	C→G	100.0%	51.5	14	intergenic (+40/‑126)	ccmH/paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  new base (0/14):  ref base (0/0):  total (0/14)

GGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACG  >  NC_014034/3333397‑3333558                                                                                                   |                                                                 ggtgccggtgcTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGgggggc                                                               <  2:3342557/101‑1 (MQ=255) ggtgccggtgcTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGgggggc                                                               <  1:753591/101‑1 (MQ=255)  gtgccggtgcTCTCGGGCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGgggggcg                                                              <  1:1310375/101‑2 (MQ=255)  gtgccggtgcTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGTCCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                              <  2:1412881/101‑1 (MQ=255)  gtgccggtgcTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                              <  1:779527/101‑1 (MQ=255)    gccggtgcTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                            <  1:4295944/101‑1 (MQ=255)     ccggtgcTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCg                                                           <  1:2664034/101‑1 (MQ=255)       ggtgcTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcc                                                         <  2:4283437/101‑1 (MQ=255)           ctctCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                     <  1:142777/101‑1 (MQ=255)            tctcGGCCGAGGAAGAGNCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCg                                                    <  2:644688/101‑1 (MQ=255)                  ccGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                              <  1:752654/101‑1 (MQ=255)                   ggAGGAAG.GGCGCGGGCTGAAAGCCCTGCTGAAGGCCTGGGGATTCCGCCCGGGGCTTCCGCCCGGGGCGCCCCGCGGGGGGCTCCGCCCCCCGCAccccc                                             <  1:3892412/100‑1 (MQ=18)                    gAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccccc                                            <  2:4199284/101‑1 (MQ=255)                       gAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCggg                                         <  2:1438023/101‑1 (MQ=255)                                    cTGGAAGGCCTGCCGAAGGGCTAGGGATTTCGCCCGGGGCTTGCGCCCCGGCCGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCa                             <  2:4266076/101‑1 (MQ=25)                                           aCCTGGTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGaaaa                      <  2:805366/101‑1 (MQ=255)                                                               tttCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACg  <  2:1549613/101‑1 (MQ=255)                                                                                                   |                                                                 GGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGC.GGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACG  >  NC_014034/3333397‑3333558 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 37 ≤  ATCG