Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,342,566	C→G	100%	R154P (CGG→CCG)	RCAP_rcc03145 ←	AsnC/Lrp family transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,342,566	0	C→G	100.0%	78.2	20	R154P (CGG→CCG)	RCAP_rcc03145	AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (0/20):  ref base (0/0):  total (0/20)

TCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGC  >  NC_014034/3342469‑3342592                                                                                                  |                           tctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                         <  1:2648251/101‑1 (MQ=255)    cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                      <  1:4430873/101‑1 (MQ=255)     ggggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccg                     <  1:1219066/101‑1 (MQ=255)       ggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGGGCACGACGCCCGGCACCGGGGGCgcggcgggcgcg                   >  1:3173736/1‑101 (MQ=255)       ggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgcg                   <  1:4140441/101‑1 (MQ=255)         cTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCggg                 <  2:2466954/101‑1 (MQ=255)            aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg              <  2:534769/101‑1 (MQ=255)            aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg              <  1:691401/101‑1 (MQ=255)             tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc             <  1:4184514/101‑1 (MQ=255)              ctcGTTCAGCGAGGGTTCCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACTCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCg            <  2:2662059/101‑1 (MQ=255)                cGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGc          <  2:662381/101‑1 (MQ=255)                cGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGc          <  2:2947797/101‑1 (MQ=255)                  ttCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcgg        <  1:1480523/101‑3 (MQ=255)                  ttCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcgg        <  2:4030656/101‑3 (MQ=255)                   tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc       <  1:758269/101‑4 (MQ=255)                   tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc       <  1:3189169/101‑4 (MQ=255)                     aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac     <  2:1720571/101‑6 (MQ=255)                     aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac     <  2:1723479/101‑6 (MQ=255)                      gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc    <  2:1002108/101‑7 (MQ=255)                       ggAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg   <  2:3059961/100‑8 (MQ=255)                       ggAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg   <  1:1113970/100‑8 (MQ=255)                       cGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg   <  2:1746550/101‑8 (MQ=255)                        gAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccgg  <  2:3187377/101‑9 (MQ=255)                        gAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccgg  <  1:1701810/101‑9 (MQ=255)                        gAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccgg  <  1:1122402/101‑9 (MQ=255)                                                                                                  |                           TCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGC  >  NC_014034/3342469‑3342592 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG