Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,880 G→A 100% P228L (CCG→CTG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,8800G→A100.0% 677.4 178P228L (CCG→CTG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (86/92):  ref base (0/0):  total (86/92)

Only 100 of 184 total aligned reads displayed.

CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977
                                                                                                  |                                                                                                 
cGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACagg                                                                                                 >  2:183127/1‑101 (MQ=255)
  gCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCGCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               <  1:541731/101‑1 (MQ=255)
  gCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               <  2:943771/101‑1 (MQ=255)
  gCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               <  2:1885539/101‑1 (MQ=255)
  gCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               >  1:3272599/1‑101 (MQ=255)
   cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  2:1253974/101‑1 (MQ=255)
      gTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTc                                                                                           >  2:2339471/1‑101 (MQ=255)
      gTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTc                                                                                           <  2:2132507/101‑1 (MQ=255)
       tttGCCCTTGCCGCCGCGGCGGCGTCGCCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCg                                                                                          <  2:2815953/101‑1 (MQ=255)
        ttGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCga                                                                                         >  1:4207988/1‑101 (MQ=255)
        ttGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCga                                                                                         >  2:4422841/1‑101 (MQ=255)
         tGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgag                                                                                        <  1:1887403/101‑1 (MQ=255)
         tGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgag                                                                                        <  2:3593795/101‑1 (MQ=255)
          gCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgaga                                                                                       <  2:166533/101‑1 (MQ=255)
            ccTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAt                                                                                     >  2:1605270/1‑101 (MQ=255)
               tGCCGCCGCCGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  <  2:1596088/101‑1 (MQ=255)
                gccgccGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgt                                                                                 <  1:3647966/101‑1 (MQ=255)
                gccgccGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgt                                                                                 >  2:3031492/1‑101 (MQ=255)
                 ccgccgCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgtc                                                                                <  1:162710/101‑1 (MQ=255)
                  cgccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtggtcg                                                                               >  1:472696/1‑101 (MQ=255)
                   gccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCgg                                                                              <  2:3729204/101‑1 (MQ=255)
                     cgcgGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTAGTCGGCg                                                                            >  2:4148959/1‑101 (MQ=255)
                     cgcgGCCGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCg                                                                            <  1:722462/101‑1 (MQ=255)
                        ggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAg                                                                         >  2:1700658/1‑101 (MQ=255)
                         gcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAgg                                                                        >  2:3778322/1‑101 (MQ=255)
                            gcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCt                                                                     <  1:2389420/101‑1 (MQ=255)
                             cgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGcccc                                                                    >  1:1969161/1‑99 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:3139946/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:2339621/101‑1 (MQ=255)
                                 ggCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAg                                                                <  2:2223026/101‑1 (MQ=255)
                                  gCTGCCGCCTCCGCCCTTTTTCACCCCCCCCGCGCCCACCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGc                                                               <  1:3059492/101‑1 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             >  1:9056/1‑101 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             <  1:60790/101‑1 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            >  2:3216916/1‑101 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            <  1:1226435/101‑1 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            <  1:3872730/101‑1 (MQ=255)
                                      ccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGa                                                           >  2:3403122/1‑101 (MQ=255)
                                        gccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAgg                                                         >  1:8807/1‑101 (MQ=255)
                                          cTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTg                                                       >  2:3915637/1‑101 (MQ=255)
                                          cTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTg                                                       >  2:4045245/1‑101 (MQ=255)
                                           tCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGa                                                      <  1:3023398/101‑1 (MQ=255)
                                           tCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGa                                                      >  1:3399058/1‑101 (MQ=255)
                                           tCCGCCCTTTTTCATCACCCCCGCGACCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGa                                                      <  1:2142995/101‑1 (MQ=255)
                                            ccGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAt                                                     >  2:748369/1‑101 (MQ=255)
                                              gCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAtct                                                   >  1:481643/1‑101 (MQ=255)
                                                  tttttCATCCCCCCCGCGCCCATCGCTTACCCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                               <  1:1780570/101‑1 (MQ=255)
                                                  tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                               <  1:1299316/101‑1 (MQ=255)
                                                  tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                               <  1:4423113/101‑1 (MQ=255)
                                                   ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                              >  1:2424587/1‑101 (MQ=255)
                                                    tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                             <  2:1364703/101‑1 (MQ=255)
                                                      tcatccCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                           <  1:50348/101‑1 (MQ=255)
                                                      tcatcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                           >  2:1943706/1‑101 (MQ=255)
                                                       catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                          >  2:2705140/1‑101 (MQ=255)
                                                        atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         <  2:2389249/101‑1 (MQ=255)
                                                        atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         >  2:2532067/1‑101 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       <  2:3456223/101‑1 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       >  2:2627761/1‑101 (MQ=255)
                                                            cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCAGAc                                     <  1:3395631/101‑1 (MQ=255)
                                                             ccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACg                                    <  1:798048/101‑1 (MQ=255)
                                                               ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                  >  2:3952102/1‑101 (MQ=255)
                                                               ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                  <  2:472644/101‑1 (MQ=255)
                                                                cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                 <  1:4439878/101‑1 (MQ=255)
                                                                cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                 <  2:1467106/101‑1 (MQ=255)
                                                                cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                 >  1:1540453/1‑101 (MQ=255)
                                                                cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                 >  1:3470867/1‑101 (MQ=255)
                                                                 gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                >  2:4284491/1‑101 (MQ=255)
                                                                  cgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGc                               <  2:3398821/101‑1 (MQ=255)
                                                                   gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                              >  1:288255/1‑101 (MQ=255)
                                                                    cccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                             >  2:2979425/1‑101 (MQ=255)
                                                                     ccATCGCTTACGCGTCCAGCTGGAGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGAGATCTTGTCGGCGTCGGACGAGAGCttg                            >  1:2502744/1‑101 (MQ=255)
                                                                      cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                           <  1:4369980/101‑1 (MQ=255)
                                                                      cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAAAGCttgt                           >  2:1775371/1‑101 (MQ=255)
                                                                         cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                        <  2:2681016/101‑1 (MQ=255)
                                                                         cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                        <  1:94666/101‑1 (MQ=255)
                                                                          gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                       <  1:2999791/101‑1 (MQ=255)
                                                                          gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                       >  2:1292993/1‑101 (MQ=255)
                                                                          gCTTACGCCTCCAGCTGGCGCGACAGGATGGTCAAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                       <  1:3848272/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  2:2804883/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:998004/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:2979646/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:4033844/101‑1 (MQ=255)
                                                                            ttACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                     >  2:1339708/1‑101 (MQ=255)
                                                                            ttACGCGTCCAGATGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                     >  1:2419540/1‑101 (MQ=255)
                                                                             tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                    >  1:1839244/1‑101 (MQ=255)
                                                                               cgcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGa                  <  2:2241819/101‑1 (MQ=255)
                                                                                gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                 >  1:1158374/1‑101 (MQ=255)
                                                                                gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                 >  2:3511557/1‑101 (MQ=255)
                                                                                gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                 <  2:2424430/101‑1 (MQ=255)
                                                                                 cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  1:3329845/101‑1 (MQ=255)
                                                                                 cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  2:874951/101‑1 (MQ=255)
                                                                                   tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:323563/101‑1 (MQ=255)
                                                                                    ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc             <  1:385704/101‑1 (MQ=255)
                                                                                    ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc             <  1:1864207/101‑1 (MQ=255)
                                                                                        cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         <  1:2769386/101‑1 (MQ=255)
                                                                                         tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg        >  1:4281179/1‑101 (MQ=255)
                                                                                         tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg        >  2:3848986/1‑101 (MQ=255)
                                                                                            cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt     >  2:2472900/1‑101 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:3920428/101‑1 (MQ=255)
                                                                                              cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg   >  1:2464871/1‑101 (MQ=255)
                                                                                               gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc  <  2:61754/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG