Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,929 C→T 100% E212K (GAG→AAG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,9290C→T100.0% 674.4 170E212K (GAG→AAG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (91/79):  ref base (0/0):  total (91/79)

Only 100 of 198 total aligned reads displayed.

TTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398832‑3399026
                                                                                                 |                                                                                                 
tttttCATCCCCCCCGCGCCCATCGCTTACCCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                <  1:1780570/101‑1 (MQ=255)
tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                >  1:3022681/1‑101 (MQ=255)
tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                <  1:4423113/101‑1 (MQ=255)
  tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                                                                              <  2:1364703/101‑1 (MQ=255)
   ttCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                                                                             <  1:2022149/101‑1 (MQ=255)
    tcatccCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                                                                            <  1:50348/101‑1 (MQ=255)
      atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          <  1:1900200/101‑1 (MQ=255)
      atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          >  2:2532067/1‑101 (MQ=255)
       tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcg                                                                                         <  2:3297450/101‑1 (MQ=255)
       tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCCCGTAGCCCGAGGTGATCTtgtcggcgtcg                                                                                         >  1:979319/1‑101 (MQ=255)
          cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                                                                      <  1:3395632/101‑1 (MQ=255)
             ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                                                                   <  2:472644/101‑1 (MQ=255)
              cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  2:1467106/101‑1 (MQ=255)
              cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  >  1:3470867/1‑101 (MQ=255)
              cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  1:4439878/101‑1 (MQ=255)
               gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                                                                 >  2:4284491/1‑101 (MQ=255)
                 gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                                                                               >  1:288255/1‑101 (MQ=255)
                  cccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                                                                              >  2:2979425/1‑101 (MQ=255)
                  cccAGCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                                                                              <  2:2352963/101‑1 (MQ=255)
                   ccATCGCTTACGCGTCCAGCTGGAGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGAGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             >  1:2502744/1‑101 (MQ=255)
                    cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                                                                            >  1:2424535/1‑101 (MQ=255)
                    cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAAAGCttgt                                                                            >  2:1775371/1‑101 (MQ=255)
                       cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                                                                         <  2:2681016/101‑1 (MQ=255)
                       cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                                                                         <  1:94666/101‑1 (MQ=255)
                        gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        >  2:2615545/1‑101 (MQ=255)
                        gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        >  2:1292993/1‑101 (MQ=255)
                        gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        <  1:2435758/101‑1 (MQ=255)
                         cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                                                                       <  2:2804883/101‑1 (MQ=255)
                           tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                                                                     >  1:1839244/1‑101 (MQ=255)
                             cgcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGa                                                                   <  2:2241819/101‑1 (MQ=255)
                              gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  <  2:2424430/101‑1 (MQ=255)
                              gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  >  2:3511557/1‑101 (MQ=255)
                               cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                                                                 <  2:874951/101‑1 (MQ=255)
                               cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                                                                 <  1:3329845/101‑1 (MQ=255)
                                gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa                                                                >  2:1445386/1‑101 (MQ=255)
                                gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa                                                                >  1:1559906/1‑101 (MQ=255)
                                 tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  2:323563/101‑1 (MQ=255)
                                 tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               >  2:776270/1‑101 (MQ=255)
                                  ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc                                                              <  1:385704/101‑1 (MQ=255)
                                     gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc                                                           >  2:3920174/1‑101 (MQ=255)
                                     gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc                                                           <  1:1301250/101‑1 (MQ=255)
                                     gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc                                                           <  1:776350/101‑1 (MQ=255)
                                      cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          <  1:2769386/101‑1 (MQ=255)
                                       tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg                                                         >  1:4281179/1‑101 (MQ=255)
                                         gcgcGACAGGGTGGTCGAGAATTCGTCGGCGGAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCTGGGa                                                       <  2:908197/101‑1 (MQ=255)
                                          cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt                                                      >  2:2472900/1‑101 (MQ=255)
                                           gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc                                                     <  1:3920428/101‑1 (MQ=255)
                                            cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg                                                    <  2:4136679/101‑1 (MQ=255)
                                            cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg                                                    >  1:2464871/1‑101 (MQ=255)
                                             gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc                                                   <  2:61754/101‑1 (MQ=255)
                                              acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  >  2:992302/4‑101 (MQ=255)
                                               caggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcgg                                                 >  1:2576291/3‑101 (MQ=255)
                                               caggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcgg                                                 >  2:1709199/3‑101 (MQ=255)
                                                aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                >  1:3640350/2‑101 (MQ=255)
                                                 ggAGGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggcg                                               <  1:1592725/101‑1 (MQ=255)
                                                  gATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGa                                              >  2:3959784/1‑101 (MQ=255)
                                                    tGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACa                                            <  2:860176/101‑1 (MQ=255)
                                                    tGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACa                                            <  1:4349409/101‑1 (MQ=255)
                                                      gTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAg                                          <  2:3468225/101‑1 (MQ=255)
                                                      gTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCCTACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAg                                          <  2:3854110/101‑1 (MQ=255)
                                                       gcGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAgg                                         <  2:4059395/100‑1 (MQ=255)
                                                        cGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGc                                        <  2:3041863/101‑1 (MQ=255)
                                                         gagaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGcc                                       >  2:3364835/1‑101 (MQ=255)
                                                          agaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCg                                      <  2:7388/101‑1 (MQ=255)
                                                           gaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGa                                     >  1:1109525/1‑101 (MQ=255)
                                                           gaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGa                                     <  1:1652011/101‑1 (MQ=255)
                                                            gaTTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGAt                                    <  1:1228866/100‑1 (MQ=255)
                                                            aaTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGAt                                    >  2:1661765/1‑101 (MQ=255)
                                                             aTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATc                                   >  1:1753060/1‑101 (MQ=255)
                                                              ttCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCg                                  <  2:3438389/101‑1 (MQ=255)
                                                               tcgtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGc                                 >  1:1370293/1‑101 (MQ=255)
                                                                cgtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGcc                                >  2:930197/1‑101 (MQ=255)
                                                                cgtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGcc                                >  1:2825386/1‑101 (MQ=255)
                                                                 gtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCg                               >  1:1488270/1‑101 (MQ=255)
                                                                 gtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCg                               <  2:1624005/101‑1 (MQ=255)
                                                                     gCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGc                           >  1:2406663/1‑101 (MQ=255)
                                                                      cGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCTGCGACAAGGCCGATAGCCGTggga                          >  2:2122845/1‑99 (MQ=255)
                                                                      cGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcc                          <  1:4079287/101‑1 (MQ=255)
                                                                       gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         <  1:132913/101‑1 (MQ=255)
                                                                       gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  2:200757/1‑101 (MQ=255)
                                                                       gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  1:1186427/1‑101 (MQ=255)
                                                                         aGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacc                       <  2:384640/101‑1 (MQ=255)
                                                                          ggCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacca                      <  2:2875919/101‑1 (MQ=255)
                                                                            ccTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgc                    >  1:1953802/1‑101 (MQ=255)
                                                                             cTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCCCCAgcg                   >  1:845066/1‑101 (MQ=255)
                                                                                ctAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCt                <  1:22631/100‑1 (MQ=255)
                                                                                 tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTc               >  1:568391/1‑101 (MQ=255)
                                                                                 tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGACACCAGCGCCTc               <  1:1230580/101‑1 (MQ=255)
                                                                                  aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              <  2:2172780/101‑1 (MQ=255)
                                                                                       gAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGAt         >  2:4024250/1‑101 (MQ=255)
                                                                                       gAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGAt         <  1:1011565/101‑1 (MQ=255)
                                                                                         ggTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATcc       <  1:1289940/101‑1 (MQ=255)
                                                                                         ggTGATCTTGTCGGAGTCGGACGAGAGCTTGTTCTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATcc       <  1:3849239/101‑1 (MQ=255)
                                                                                          gTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATccc      <  2:1898936/101‑1 (MQ=255)
                                                                                          gTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATccc      <  2:1269175/101‑1 (MQ=255)
                                                                                           tGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATcccc     >  2:3761778/1‑101 (MQ=255)
                                                                                           tGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATcccc     <  1:299361/101‑1 (MQ=255)
                                                                                           tGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATcccc     >  1:2192407/1‑101 (MQ=255)
                                                                                            gATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCg    <  2:4426457/101‑1 (MQ=255)
                                                                                              tcttGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGgc  <  2:2289408/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
TTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398832‑3399026

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG