Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,382 C→A 90.9% intergenic (‑92/+38) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3820C→A90.9% 14.2 ‑2.3 ‑0.9103intergenic (‑92/+38)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (47/45):  ref base (8/2):  total (56/47)
Fisher's exact test strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.16e-01

Only 100 of 151 total aligned reads displayed.

CTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461284‑3461461
                                                                                                     |                                                                                
cTTTCATCGCAAGGCCCCCTTTTCGCTCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGcca                                                                                 <  1:233802/101‑2 (MQ=255)
cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGcca                                                                                 <  2:2391141/101‑2 (MQ=255)
cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGcca                                                                                 >  1:2663269/1‑100 (MQ=255)
 tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccac                                                                                >  2:512551/1‑99 (MQ=255)
 tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccac                                                                                <  2:1503820/101‑3 (MQ=255)
 tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccac                                                                                <  1:4034899/101‑3 (MQ=255)
  ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGTAG.GG.GGCGCAGccacc                                                                               <  2:2592178/101‑4 (MQ=255)
  ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacc                                                                               >  1:3579669/1‑98 (MQ=255)
  ttCAGCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacc                                                                               <  1:2431777/101‑4 (MQ=255)
   tCATCGCAAGGCCCCCTTTTCGCACCCGCAGTTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccaccc                                                                              >  1:3977250/1‑97 (MQ=255)
   tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccaccc                                                                              <  1:2965627/101‑5 (MQ=255)
   tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccaccc                                                                              >  2:1778441/1‑97 (MQ=255)
   tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccaccc                                                                              >  2:663370/1‑97 (MQ=255)
    cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGTGG.GC.GCAGccacccg                                                                             <  1:3749461/101‑6 (MQ=255)
    cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccg                                                                             <  1:3106293/101‑6 (MQ=255)
     aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccgg                                                                            >  1:3999888/1‑95 (MQ=255)
     aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccgg                                                                            <  2:1574197/101‑7 (MQ=255)
     aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGCTTGGCGCAGGGCCAAAGGAAG.GGCGC.GCAGccacccgg                                                                            >  2:2208816/1‑95 (MQ=255)
     aTCGCAAGGCCCCCGTTTCGCACCCGCAGGCTAGCGGGATCCACGGGCGCGGCCAGGCGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGCCGCccgg                                                                            <  2:737691/101‑3 (MQ=255)
      tCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggc                                                                           <  2:3489920/101‑8 (MQ=255)
       cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggcc                                                                          <  1:718726/101‑9 (MQ=255)
       cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggcc                                                                          >  2:4335737/1‑93 (MQ=255)
       cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggcc                                                                          >  1:3044255/1‑93 (MQ=255)
       cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggcc                                                                          >  1:3005057/1‑93 (MQ=255)
       cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggcc                                                                          <  1:2881134/101‑9 (MQ=255)
        gCAAGGCCCCCTTTTCGCACCCTCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggccg                                                                         <  2:717123/101‑10 (MQ=255)
        gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggccg                                                                         >  2:1243038/1‑92 (MQ=255)
         cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGccacccggccgc                                                                        >  2:1484451/1‑91 (MQ=255)
                       cGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGCC.ACCCGGCCGGgcccc.caaccccgg                                                        >  1:3733976/1‑93 (MQ=255)
                           cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                <  2:2107219/101‑1 (MQ=255)
                            ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               <  2:2858808/101‑1 (MQ=255)
                            ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               <  2:2679688/101‑1 (MQ=255)
                            ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               >  2:533440/1‑101 (MQ=255)
                            ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               <  2:4343925/101‑1 (MQ=255)
                              gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                             <  2:1952006/101‑1 (MQ=255)
                               cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            <  1:2517397/101‑1 (MQ=255)
                               cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            >  2:2947658/1‑101 (MQ=255)
                               cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            <  1:663438/101‑1 (MQ=255)
                                aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           >  2:202186/1‑101 (MQ=255)
                                aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           <  2:3413417/101‑1 (MQ=255)
                                aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           <  1:3417163/101‑1 (MQ=255)
                                 gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          >  2:4416418/1‑101 (MQ=255)
                                 gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          <  2:4437657/101‑1 (MQ=255)
                                 gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GC..AGCCAACC.GGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          >  1:3211255/1‑101 (MQ=255)
                                  cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                         <  1:4174863/101‑1 (MQ=255)
                                  cTTAGAAAGATGCGCGGGGCCGCCCCGCCGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GGCCCAGCC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCa                                         <  2:567523/101‑1 (MQ=255)
                                    tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       <  1:1442277/101‑1 (MQ=255)
                                    tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       <  1:520629/101‑1 (MQ=255)
                                      gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                     >  2:3800219/1‑101 (MQ=255)
                                        aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                   <  2:3955126/101‑1 (MQ=255)
                                          gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                 >  1:2642963/1‑101 (MQ=255)
                                           aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:1754427/1‑101 (MQ=255)
                                           aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  2:2675822/1‑101 (MQ=255)
                                           aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:566592/1‑101 (MQ=255)
                                            tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  2:3434680/1‑101 (MQ=255)
                                            tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:1244882/101‑1 (MQ=255)
                                            tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  1:4204945/1‑101 (MQ=255)
                                            tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  1:2194690/1‑101 (MQ=255)
                                            tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:51690/101‑1 (MQ=255)
                                              cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  1:3282765/101‑1 (MQ=255)
                                              cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  2:2192517/101‑1 (MQ=255)
                                               aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                            >  1:1807860/1‑101 (MQ=255)
                                               aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                            >  2:3756480/1‑101 (MQ=255)
                                                cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGCTCAGTTCTTGCcgacga                           >  1:743932/1‑101 (MQ=255)
                                                 gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  1:3089612/1‑101 (MQ=255)
                                                 gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  1:485732/1‑101 (MQ=255)
                                                  ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         >  1:821679/1‑101 (MQ=255)
                                                  ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         <  1:2586714/101‑1 (MQ=255)
                                                   gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCTGGTCGTTCGATCAGTTCTTGCCGACGATGa                        <  2:3976994/101‑1 (MQ=255)
                                                    cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                       >  1:3910591/1‑101 (MQ=255)
                                                     gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                      <  2:698467/101‑1 (MQ=255)
                                                     gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                      <  1:4051574/101‑1 (MQ=255)
                                                      cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                     <  2:2883855/101‑1 (MQ=255)
                                                       ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    >  2:2125424/1‑101 (MQ=255)
                                                       ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    >  2:1009380/1‑101 (MQ=255)
                                                       ggCCAGACGCAGTTTGGCCCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    <  1:1693145/101‑1 (MQ=255)
                                                        gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                   >  1:4394634/1‑101 (MQ=255)
                                                        gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..AG.GG.GC.GC..AACCAACC.GGCGGCGGCCCCAACGCCGGTCGTTCGCTCAGTTCTTGCCGCCGCTGACGTCa                   >  2:2178736/1‑101 (MQ=255)
                                                          cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                 <  1:3936270/101‑1 (MQ=255)
                                                          cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                 <  1:1596684/101‑1 (MQ=255)
                                                            gACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg               >  1:276565/1‑101 (MQ=255)
                                                            gACGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg               >  2:4120964/1‑101 (MQ=255)
                                                             aCGCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCTGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc              <  2:2295402/101‑1 (MQ=255)
                                                               gCAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt            >  1:4432896/1‑101 (MQ=255)
                                                                cAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           >  2:1557234/1‑101 (MQ=255)
                                                                cAGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           <  1:274372/101‑1 (MQ=255)
                                                                 aGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg          <  1:913877/101‑1 (MQ=255)
                                                                 aGTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg          >  1:3418041/1‑101 (MQ=255)
                                                                  gTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  2:352415/1‑101 (MQ=255)
                                                                  gTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  1:1232571/1‑101 (MQ=255)
                                                                  gTTTGGCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  1:1066661/1‑101 (MQ=255)
                                                                     tGGCGCAGGGCCAAATGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGTTCGATCAGTTCTTGCCGACGGTGACATCAGGGACACtgatga      >  2:2994113/1‑101 (MQ=255)
                                                                     tGGCGCAGGGCCAAAGGAAG.GGAGC.GCAGCC.ACCCTGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      <  2:743852/101‑1 (MQ=255)
                                                                     tGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCCGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  2:1792325/1‑101 (MQ=255)
                                                                     tGGCGCAGGGCCAAAGGAAG.GG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  1:270951/1‑101 (MQ=255)
                                                                      ggCGCAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat     <  2:2798682/101‑1 (MQ=255)
                                                                        cgcAGGGCCAAAGGAAG.GG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg   >  1:1282155/1‑101 (MQ=255)
                                                                        cgcAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg   >  2:2880198/1‑101 (MQ=255)
                                                                         gcAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  >  2:4175234/1‑101 (MQ=255)
                                                                         gcAGGGCCAAAGGAA..GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  <  1:363640/101‑1 (MQ=255)
                                                                                                     |                                                                                
CTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.GG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461284‑3461461

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG