Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,481,897 G→C 3.1% G20R (GGC→CGC)  rnfB → electron transport complex protein RnfB

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,481,8970G→C 3.1% 3.2 ‑4.1 0.0189G20R (GGC→CGC) rnfBelectron transport complex protein RnfB
Reads supporting (aligned to +/- strand):  new base (7/0):  ref base (95/87):  total (102/87)
Fisher's exact test strand distribution p-value = 1.59e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

TTGCCGGGCTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCTTTCCCG  >  NC_014034/3481797‑3481996
                                                                                                    |                                                                                                   
ttGCCGGGCTCGTGCACGTCTGATCGTCGAAAGGAGCCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCg                                                                                                     <  2:4260220/101‑1 (MQ=255)
 tGCCGGGCTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCgg                                                                                                    >  2:3256594/1‑101 (MQ=255)
  gCCGGGCTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTTCTCGgc                                                                                                   >  1:725736/1‑101 (MQ=255)
    cGGGCTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGgcgc                                                                                                 >  1:2882257/1‑101 (MQ=255)
     gggCTCGTGCCCGTCTGATCGTCGAAAGGAGCCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGcgcc                                                                                                <  2:932080/101‑1 (MQ=255)
     gggCTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGcgcc                                                                                                >  2:1091466/1‑101 (MQ=255)
       gCTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGcgccgc                                                                                              >  2:2640233/1‑101 (MQ=255)
        cTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGcgccgcc                                                                                             >  1:3289154/1‑101 (MQ=255)
        cTCGGGCACGTCTGATCGTGGAAGGGAGCCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGcgccgcc                                                                                             <  1:3089085/101‑1 (MQ=255)
         tCGGGCACGTGTGTTCGTGAAAGGGAGCCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGcgccgccg                                                                                            <  2:1339279/101‑1 (MQ=255)
          cGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGcgccgccgc                                                                                           >  1:2755692/1‑101 (MQ=255)
                 tttggaccgtcaAAAGAAGCCCTGCCATGAACGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAg                                                                                    <  1:1996293/94‑1 (MQ=255)
                 gTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAg                                                                                    >  1:3359512/1‑101 (MQ=255)
                  tgtggccgtggAAGGGAGCCCTGCCATGATCCCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGt                                                                                   <  1:2043466/95‑1 (MQ=255)
                  ccTGATCGTCGAAAGGAGCCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGt                                                                                   <  1:1946645/100‑1 (MQ=255)
                   cTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGtt                                                                                  >  2:3538554/1‑101 (MQ=255)
                    tGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTc                                                                                 >  2:4350130/1‑101 (MQ=255)
                      aTCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCa                                                                               >  1:1348053/1‑101 (MQ=255)
                       tcgtcgAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCAc                                                                              >  1:3716136/1‑101 (MQ=255)
                        cgtcgAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACg                                                                             >  1:2520882/1‑101 (MQ=255)
                         ggcaaaaGGAGCCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGt                                                                            <  2:2284346/97‑1 (MQ=255)
                          cggAAGGGAGCCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTc                                                                           <  2:2717209/99‑1 (MQ=255)
                           ggaagggaccccTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCg                                                                          <  2:1003581/91‑1 (MQ=255)
                           cgAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCg                                                                          >  1:3416405/1‑101 (MQ=255)
                            gAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCga                                                                         >  2:1309640/1‑101 (MQ=255)
                            gAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCga                                                                         >  1:3658802/1‑101 (MQ=255)
                             aaaGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCgag                                                                        >  2:1919497/1‑101 (MQ=255)
                              agggggcccTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCgaga                                                                       <  1:1241968/94‑1 (MQ=255)
                              aaGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCgaga                                                                       >  1:4340490/1‑101 (MQ=255)
                              aaGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCgaga                                                                       >  1:1576695/1‑101 (MQ=255)
                              aaGGAGACCTGCCATGATCGCAGCCGCCGCTTCAATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCCGCGCCCCCCCCCCCCAGCTCCCCGCCgcga                                                                       >  2:1673917/1‑101 (MQ=255)
                                  ggcccTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGAcccc                                                                   <  2:3288922/98‑1 (MQ=255)
                                  agcccTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGAcccc                                                                   <  1:4210104/98‑1 (MQ=255)
                                    aCCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCccgc                                                                 >  2:679145/1‑101 (MQ=255)
                                     ccTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCccgcc                                                                <  2:2061959/101‑1 (MQ=255)
                                     ccTCCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCccgcc                                                                >  2:3772114/1‑101 (MQ=255)
                                      cTGCCATGATCGCCGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCccgccg                                                               <  1:1107893/101‑1 (MQ=255)
                                      cTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCccgccg                                                               >  1:4303332/1‑101 (MQ=255)
                                       tGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGa                                                              >  1:1644757/1‑101 (MQ=255)
                                        gCCATGACCGCGCCCCCCGCTTCCGTGTCGCCCCTCGGTCTGGGGCTGGGCTCCCTGCTCGCCGCCGCCCCCCGCAAGTTCCACGTCGAGCCCCCGCCGAt                                                             <  2:803148/101‑1 (MQ=255)
                                         ccATGCTCGCCGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGAtc                                                            <  2:3214835/101‑1 (MQ=255)
                                          cATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGAtcg                                                           >  2:4166197/1‑101 (MQ=255)
                                           aTGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGAtcgt                                                          >  1:2454112/1‑101 (MQ=255)
                                             gATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGAtcgtcg                                                        <  2:2755502/101‑1 (MQ=255)
                                             gATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGAtcgtcg                                                        <  2:2447383/101‑1 (MQ=255)
                                              acccccgccgccGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGa                                                       <  1:1523626/95‑1 (MQ=255)
                                              aTCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCACCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGa                                                       >  1:3815070/1‑101 (MQ=255)
                                                cGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAgg                                                     >  2:2571593/1‑101 (MQ=255)
                                                  cAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGaa                                                   >  2:3474942/1‑101 (MQ=255)
                                                     cagccgcTCCCATGACGGGCCTGGGGCTCGGGCTGGGGTATCTGCTCGGCGCCGCTGCCCGCAAGTTCCACGTCGGGACCCCGCCTATCGTCGAGGAAATc                                                <  2:2943478/99‑1 (MQ=255)
                                                       gccgcTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGc                                              >  2:3605216/1‑101 (MQ=255)
                                                       gccgcTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCCGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCCATCGTCGGGGAGATAGc                                              >  2:1153981/1‑101 (MQ=255)
                                                        ccgcTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGcc                                             <  2:1347956/101‑1 (MQ=255)
                                                        ccgcTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGcc                                             <  1:4403113/101‑1 (MQ=255)
                                                          gcTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCaa                                           >  1:3019874/1‑101 (MQ=255)
                                                           cTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAg                                          >  2:3352762/1‑101 (MQ=255)
                                                            ttCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGa                                         <  2:910369/101‑1 (MQ=255)
                                                            ttCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGa                                         >  2:3679541/1‑101 (MQ=255)
                                                             tCCATGTCGGCCGTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGAt                                        >  2:4146467/1‑101 (MQ=255)
                                                                aTGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCt                                     <  1:4438405/101‑1 (MQ=255)
                                                                 tGTCGGCCCTGGGGCTCGGGCTGGGCTCCCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTg                                    <  1:3900761/101‑1 (MQ=255)
                                                                 tGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTg                                    >  1:2141374/1‑101 (MQ=255)
                                                                 tGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCCCCCGCCAGTTCCCCGTCGTGACCCCGCCGTTTGCCTAGGGAATCGCCACGACCCtc                                    >  2:407834/1‑100 (MQ=255)
                                                                 tGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCCCCGCCCCCGCCCCCAAGATTCACCTCCTGCCCCCCCCGCTCGCCGAAGAAAACGCCAACATACtc                                    >  2:2983701/1‑100 (MQ=255)
                                                                   tCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGcc                                  <  2:1495662/101‑1 (MQ=255)
                                                                   tCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGcc                                  <  2:1234191/101‑1 (MQ=255)
                                                                   gcGGCCCTCGGGCTCGGGCTGGGCTCCCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGcc                                  <  1:808428/100‑1 (MQ=255)
                                                                     ggCCCTGGGGCTCGGGCTGGCCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCg                                <  1:1835898/101‑1 (MQ=255)
                                                                        ccTCGGGCTGGGGCTGGGCTCCCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCa                             <  1:1339091/101‑1 (MQ=255)
                                                                         ctcgggctcgggCTGGGCTACCTGCTCCGCGCCGCCGCCCGCCAGTTCCACGTCGAGACCCCCCCGCTCGTCGAGGAAATCCCCAAGATCCTGCCCGGCAc                            >  2:531664/1‑101 (MQ=255)
                                                                         ctcgggctcgggCTGGGCTACCTGCTCCGCGCCGCCGCCCGCAAACTCCACGGCTACACCCCTCCGCTCCTTGGGGGAATCCCCCAACACATGCCCGGccc                            >  2:701152/1‑99 (MQ=255)
                                                                          tcgggctcgggcTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGCCCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCAcc                           <  2:4001748/101‑1 (MQ=255)
                                                                          tcgggctcgggcTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCAcc                           <  1:4173837/101‑1 (MQ=255)
                                                                           cgggctcgggctGGGCTACCTGCTCCGCGCCCCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAAACGCCAAGATCCTGCCCGGCACCa                          >  1:1229833/1‑101 (MQ=255)
                                                                               ctcgggctGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTg                      <  2:660787/101‑1 (MQ=255)
                                                                               ctcgggctGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTg                      <  1:69483/101‑1 (MQ=255)
                                                                                tggggctgggctCCCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTgc                     <  2:4231038/99‑1 (MQ=255)
                                                                                tcgggctGGGCTCCCTGCTCGGCGCCCCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTgc                     <  2:3019653/101‑1 (MQ=255)
                                                                                tcgggctGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTgc                     >  2:3862528/1‑101 (MQ=255)
                                                                                tcgggctGGGCTACCTGCTCGGCGCCCCCGCCCGCCAGTTCCACGTCGAGACCCCGCCGCTCGTCGAGGAAATCGCCCAGATCCTTCCCGGCACCCAtttc                     >  2:1611981/1‑99 (MQ=255)
                                                                                 cgggctGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTgcg                    >  2:2439242/1‑101 (MQ=255)
                                                                                  gggctgggctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTgcgg                   >  2:185891/1‑101 (MQ=255)
                                                                                  gggctgggctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTgcgg                   >  2:314279/1‑101 (MQ=255)
                                                                                   ggctgggctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTgcggc                  <  1:1424797/101‑1 (MQ=255)
                                                                                     ctgggctCCCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGgcgc                <  1:4024423/101‑1 (MQ=255)
                                                                                     ctgggctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGgcgc                >  2:1532105/1‑101 (MQ=255)
                                                                                      tgggctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCTCCAAGATCCTGCCCGGCACCAATTGCGGCGcc               >  2:2154827/1‑101 (MQ=255)
                                                                                      tgggctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGcc               <  1:3166193/101‑1 (MQ=255)
                                                                                      tgggctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGcc               >  2:3239774/1‑101 (MQ=255)
                                                                                      tgggctACCTGCTCGGCGCCGCCGCCCCCAAGGTCCCCGTCCAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCCGCACCAATTtcggcggc               >  2:75718/1‑99 (MQ=255)
                                                                                       gggctACCTGCTCGGCGCCCCCGCCCGCAAGTTCCACGTCGAGCCCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCt              <  2:1707588/101‑1 (MQ=255)
                                                                                         gctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGc            <  1:391856/101‑1 (MQ=255)
                                                                                          ctACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCg           >  1:2207610/1‑101 (MQ=255)
                                                                                             ctggcgcGGCGCCCCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCt        <  1:4146728/95‑1 (MQ=255)
                                                                                             ccggCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGCCCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCt        <  2:2343567/98‑1 (MQ=255)
                                                                                             ccTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCt        >  1:238964/1‑101 (MQ=255)
                                                                                              cTGCTCGGCCCCGCCGCCCGCAAGTTCCACGTCGAGCCCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCtt       <  2:3658549/101‑1 (MQ=255)
                                                                                                 cTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCTTTcc    <  2:2520719/101‑1 (MQ=255)
                                                                                                  ccGGCCCCGCCGCCCGCAAGTTCCACGTCGAGCGCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCTTTccc   <  1:3871427/100‑1 (MQ=255)
                                                                                                   cGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCTTTCCCg  >  2:25468/1‑101 (MQ=255)
                                                                                                    |                                                                                                   
TTGCCGGGCTCGTGCACGTCTGATCGTCGAAAGGAGACCTGCCATGATCGCAGCCGCCGCTTCCATGTCGGCCCTCGGGCTCGGGCTGGGCTACCTGCTCGGCGCCGCCGCCCGCAAGTTCCACGTCGAGACCCCGCCGATCGTCGAGGAAATCGCCAAGATCCTGCCCGGCACCAATTGCGGCGCCTGCGGCTTTCCCG  >  NC_014034/3481797‑3481996

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG