Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,604 T→C 100% A410A (GCT→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6040T→C100.0% 830.5 220A410A (GCT→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (116/103):  ref base (0/0):  total (117/103)

Only 100 of 247 total aligned reads displayed.

TGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531507‑3531701
                                                                                                 |                                                                                                 
  aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                              <  2:319039/101‑1 (MQ=255)
  aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                              >  1:682312/1‑101 (MQ=255)
    cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            <  1:2635176/101‑1 (MQ=255)
    cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            >  1:3152715/1‑101 (MQ=255)
     tACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGaa                                                                                           <  1:2187848/101‑1 (MQ=255)
     tACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGaa                                                                                           <  1:2154573/101‑1 (MQ=255)
      acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                          <  1:1968232/101‑1 (MQ=255)
        aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                        >  2:2594006/1‑101 (MQ=255)
        aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                        <  2:516562/101‑1 (MQ=255)
        aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                        >  1:3447181/1‑101 (MQ=255)
          caacTCGCCCAGCTTCAACTGGACGCTGACCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGc                                                                                      <  2:748587/101‑1 (MQ=255)
          caacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGc                                                                                      <  2:2736999/101‑1 (MQ=255)
              tCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGa                                                                                  <  1:514429/101‑1 (MQ=255)
              tCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGa                                                                                  <  1:1162265/101‑1 (MQ=255)
               cGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAg                                                                                 <  2:2073540/101‑1 (MQ=255)
                gCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGacc                                                                                >  2:340144/1‑99 (MQ=255)
                  ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                              >  1:1547093/1‑101 (MQ=255)
                    aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgat                                                                            >  1:2242651/1‑101 (MQ=255)
                      cTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatga                                                                          >  1:1011063/1‑101 (MQ=255)
                       ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                         >  2:3507823/1‑101 (MQ=255)
                       ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                         >  2:3517901/1‑101 (MQ=255)
                        tCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAg                                                                        <  2:1063721/101‑1 (MQ=255)
                        tCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAg                                                                        >  1:2306426/1‑101 (MQ=255)
                         cAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGc                                                                       <  1:480626/101‑1 (MQ=255)
                          aaCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGcc                                                                      >  1:708334/1‑101 (MQ=255)
                           acgggACGCTGAACCGGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGccc                                                                     <  1:1947548/98‑1 (MQ=255)
                           aCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGccc                                                                     <  2:1695591/101‑1 (MQ=255)
                           aCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGccc                                                                     <  1:792101/101‑1 (MQ=255)
                              ggACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAc                                                                  >  2:3753925/1‑101 (MQ=255)
                               ggcggtGAACCTGCGCAAGCAGTTGCGCGCCCAGTGGTTGGCCCAGGGCACGATCACCGAAGGCGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACt                                                                 <  1:2742963/96‑1 (MQ=255)
                                aCGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTa                                                                <  2:1773654/101‑1 (MQ=255)
                                aCGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTa                                                                <  2:3699799/101‑1 (MQ=255)
                                 cGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTAt                                                               <  1:4039983/101‑1 (MQ=255)
                                  gCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATg                                                              <  2:2448651/101‑1 (MQ=255)
                                  gCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATg                                                              <  1:1474655/101‑1 (MQ=255)
                                   cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCCCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATga                                                             >  1:2228018/1‑101 (MQ=255)
                                   cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGCAGACCCCTATCCGAACGGGACCGAGCGGGCTGAGCCCGACTAtca                                                             >  2:1583288/1‑99 (MQ=255)
                                   cTGAACCTGCGCAAGCAGGCGCGCGCGCAGTGGCTGGGCGAGGGGCCGGTCACCGAAGGCGCCTATCAGAACGGGCCCGAGCTGATGAAGACCGACTATga                                                             >  2:2412878/1‑101 (MQ=255)
                                    tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            >  1:4186935/1‑101 (MQ=255)
                                    tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            >  1:29739/1‑101 (MQ=255)
                                      aaCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacga                                                          >  2:1830947/1‑101 (MQ=255)
                                        ccTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGATGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGaca                                                        >  2:4281252/1‑101 (MQ=255)
                                        ccTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGaca                                                        <  2:2908402/101‑1 (MQ=255)
                                        ccTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGaca                                                        >  2:63514/1‑101 (MQ=255)
                                         cTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                       <  2:250023/101‑1 (MQ=255)
                                          tGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacacc                                                      >  2:672919/1‑100 (MQ=255)
                                          tGCCCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCGGAGCTGATGAAGCCCGACTATGACGACACg                                                      <  2:1080947/101‑1 (MQ=255)
                                           gcgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGc                                                     >  1:4377076/1‑101 (MQ=255)
                                            cgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcc                                                    >  2:4227905/1‑101 (MQ=255)
                                             gcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccc                                                   >  2:2693031/1‑101 (MQ=255)
                                             gcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccc                                                   >  2:2993086/1‑101 (MQ=255)
                                             gcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccc                                                   <  2:3116166/101‑1 (MQ=255)
                                              cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                  >  1:3284343/1‑100 (MQ=255)
                                                aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                <  2:31253/101‑1 (MQ=255)
                                                aGCAGGCGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGAAGACACGccccta                                                >  2:124197/1‑98 (MQ=255)
                                                 gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACTATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                               <  1:3836902/101‑1 (MQ=255)
                                                  cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                              <  1:3598822/101‑1 (MQ=255)
                                                  cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                              <  2:3361326/101‑1 (MQ=255)
                                                  cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                              >  2:4349362/1‑101 (MQ=255)
                                                   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                             >  1:3044699/1‑101 (MQ=255)
                                                   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                             >  1:3988008/1‑101 (MQ=255)
                                                     gTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcg                                           <  1:657699/101‑1 (MQ=255)
                                                     gTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcg                                           <  1:1768483/101‑1 (MQ=255)
                                                         gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                       <  2:1522916/101‑1 (MQ=255)
                                                         gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                       >  1:308821/1‑101 (MQ=255)
                                                         gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                       <  1:1029141/101‑1 (MQ=255)
                                                         gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                       >  1:1318713/1‑101 (MQ=255)
                                                             gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCg                                   <  2:3424698/101‑1 (MQ=255)
                                                              cttccgatctccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                  <  1:3367862/91‑1 (MQ=255)
                                                              cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                  >  2:2328671/1‑101 (MQ=255)
                                                               aGTGGCTGGCCGAGGGCACGATCCCCGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 <  2:2242506/101‑1 (MQ=255)
                                                               aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTAGCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 <  1:83341/101‑1 (MQ=255)
                                                                 tggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGc                               >  2:937118/1‑101 (MQ=255)
                                                                 tggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGc                               >  1:973690/1‑101 (MQ=255)
                                                                 tggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGc                               >  1:928304/1‑101 (MQ=255)
                                                                  ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcc                              >  1:3016209/1‑100 (MQ=255)
                                                                    ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            <  1:63527/101‑1 (MQ=255)
                                                                     tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                           >  2:2001743/1‑101 (MQ=255)
                                                                     tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                           >  1:4111547/1‑101 (MQ=255)
                                                                      ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                          >  2:72611/1‑101 (MQ=255)
                                                                       gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                         >  2:91739/1‑101 (MQ=255)
                                                                        ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                        <  1:3199589/101‑1 (MQ=255)
                                                                        ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                        >  2:387281/1‑101 (MQ=255)
                                                                         cGAGGGCACGATCCCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                       <  2:2129971/101‑1 (MQ=255)
                                                                         cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                       >  2:3588321/1‑101 (MQ=255)
                                                                           aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                     >  2:3939995/1‑101 (MQ=255)
                                                                           aGGGCACGATCACCGAAGACGACTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                     >  1:2546987/1‑101 (MQ=255)
                                                                            gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                    >  2:3168973/1‑101 (MQ=255)
                                                                             ggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGc                   <  1:3518149/101‑1 (MQ=255)
                                                                              gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                  >  1:1723370/1‑101 (MQ=255)
                                                                                    tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg            <  1:3483256/101‑1 (MQ=255)
                                                                                      aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc          >  1:4337093/1‑101 (MQ=255)
                                                                                        cGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg        <  2:1010978/101‑1 (MQ=255)
                                                                                          aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag      >  2:1669762/1‑101 (MQ=255)
                                                                                            gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt    <  2:3284111/101‑1 (MQ=255)
                                                                                            gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTAGGACGACACGCCCCGCGGCCGGGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt    <  1:2897941/101‑1 (MQ=255)
                                                                                             gcGCCTATCCGAAGGGCCCCGAGCTGATGAAGCCGGAGTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc   <  1:2413035/100‑1 (MQ=255)
                                                                                             ccGCCTATCCGACCGGCCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc   <  2:1269500/100‑1 (MQ=255)
                                                                                              cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg  >  1:2945888/1‑101 (MQ=255)
                                                                                              cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg  <  1:1606966/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
TGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531507‑3531701

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG