Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,539,498 T→G 100% D261E (GAT→GAG prpD → 2‑methylcitrate dehydratase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,539,4980T→G100.0% 869.9 238D261E (GAT→GAGprpD2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  new base (125/112):  ref base (0/0):  total (125/113)

Only 100 of 259 total aligned reads displayed.

ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595
                                                                                                  |                                                                                                 
aTCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGgc                                                                                                 >  2:1677986/1‑101 (MQ=255)
  cGAGGCGGTGCACCGCGCCCTGCGCCGGGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATCCTCTCGGGCCCAGAGgcgc                                                                                               <  2:4354829/101‑1 (MQ=255)
   gAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGcgcg                                                                                              <  1:1535593/101‑1 (MQ=255)
    aGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGt                                                                                             >  2:144020/1‑101 (MQ=255)
      cttccgatctgcgcCCTGCGCGGCGAAGGCGACCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTa                                                                                           <  1:1460112/91‑1 (MQ=255)
        ggTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTaca                                                                                         <  1:668866/101‑1 (MQ=255)
         gTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTacac                                                                                        <  2:3913685/101‑1 (MQ=255)
          tGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACAcc                                                                                       >  2:2541300/1‑101 (MQ=255)
          tGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACAcc                                                                                       <  2:1742932/101‑1 (MQ=255)
           ggACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCg                                                                                      >  1:4032640/1‑101 (MQ=255)
            gACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGt                                                                                     >  1:3863570/1‑101 (MQ=255)
            gACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGt                                                                                     <  2:2152532/101‑1 (MQ=255)
            gACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGt                                                                                     >  2:3034721/1‑101 (MQ=255)
             aCCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTc                                                                                    <  1:236155/101‑1 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   >  2:814190/1‑101 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  1:222808/101‑1 (MQ=255)
                gcgcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTaagatcggaag                                                                                 >  2:1460006/1‑91 (MQ=255)
                 cgcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTAATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGcc                                                                                >  2:3266571/1‑101 (MQ=255)
                  gcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCt                                                                               <  2:3781916/101‑1 (MQ=255)
                  gcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGACCAGAGGCGCGTTACACCGTCCGCCt                                                                               >  2:3715368/1‑101 (MQ=255)
                    ccTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGc                                                                             >  1:2969769/1‑101 (MQ=255)
                     cTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGcc                                                                            <  2:145087/101‑1 (MQ=255)
                     cTGCGCGGCGAAGGCGCCCACAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGTATGGATGATCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGcc                                                                            <  1:4418022/101‑1 (MQ=255)
                      tGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGccc                                                                           <  1:1551475/101‑1 (MQ=255)
                          cggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGcc                                                                       >  2:2775632/1‑101 (MQ=255)
                           ggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACGCCGTCCGCCTGCCCGGCCa                                                                      >  1:1705480/1‑101 (MQ=255)
                            gcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCAt                                                                     >  2:2961540/1‑101 (MQ=255)
                              gAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   >  1:3345486/1‑101 (MQ=255)
                              gAAGGCCCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   <  2:1248715/101‑1 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  <  2:1552273/101‑1 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  <  1:1956345/101‑1 (MQ=255)
                                aGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCg                                                                 >  1:1889250/1‑101 (MQ=255)
                                 ggCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGa                                                                <  1:1188698/101‑1 (MQ=255)
                                   cgcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGc                                                              >  1:4350208/1‑101 (MQ=255)
                                    gcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGcc                                                             >  1:268482/1‑101 (MQ=255)
                                      ccccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGa                                                           >  2:270176/1‑101 (MQ=255)
                                       cccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaa                                                          >  2:4226109/1‑101 (MQ=255)
                                         cAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAc                                                        <  1:1543158/101‑1 (MQ=255)
                                          tgCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       >  2:3172400/2‑101 (MQ=255)
                                          agatcggCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       >  2:342060/7‑101 (MQ=255)
                                            ccccGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcgcg                                                     >  2:1833194/1‑101 (MQ=255)
                                              ccGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGcc                                                   <  1:3367861/101‑1 (MQ=255)
                                                gCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCAt                                                 <  2:4032383/101‑1 (MQ=255)
                                                 ccTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATc                                                <  1:2588334/101‑1 (MQ=255)
                                                 ccTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATc                                                >  1:1042756/1‑101 (MQ=255)
                                                  cTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATcc                                               >  2:676829/1‑101 (MQ=255)
                                                  cTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATcc                                               >  2:4332043/1‑101 (MQ=255)
                                                  cTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATcc                                               >  2:717713/1‑101 (MQ=255)
                                                  cTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATcc                                               >  1:704732/1‑101 (MQ=255)
                                                  cTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATcc                                               >  1:3448695/1‑101 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              >  2:973056/1‑101 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              >  1:528760/1‑101 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              <  2:4065995/101‑1 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              >  2:3320246/1‑101 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              <  2:3137450/101‑1 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              <  1:3891862/101‑1 (MQ=255)
                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCCACCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             >  1:3822486/1‑101 (MQ=255)
                                                     ggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTg                                            <  1:3172627/101‑1 (MQ=255)
                                                      gAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGa                                           >  2:609203/1‑101 (MQ=255)
                                                      gAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGa                                           >  1:492581/1‑101 (MQ=255)
                                                      gAGGGGGAAGACGGTTTCATCGCCTGGATGCTCCCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGa                                           <  2:1215556/101‑1 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         >  1:2488568/1‑101 (MQ=255)
                                                        actggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         >  2:467074/4‑101 (MQ=255)
                                                         ggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAAGCGCCATCCTTGacac                                        >  1:984545/1‑101 (MQ=255)
                                                            gAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     <  1:117869/101‑1 (MQ=255)
                                                            gAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     >  1:1651184/1‑101 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   >  2:2360439/1‑101 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   >  1:2855950/1‑101 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   <  2:4205366/101‑1 (MQ=255)
                                                               gtcGGTTTCATCGCCGGGATGCGCTCGGGCCAAGAGGCGCGTTACACCGTCCCCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAc                                  <  1:1442334/99‑1 (MQ=255)
                                                                    tttCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAgg                             >  1:3436132/1‑101 (MQ=255)
                                                                     ttCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGa                            <  1:1752314/101‑1 (MQ=255)
                                                                     ttCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGa                            >  1:3940280/1‑101 (MQ=255)
                                                                     ttCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCACCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGa                            >  2:1982064/1‑101 (MQ=255)
                                                                      tCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGaa                           <  1:3638227/101‑1 (MQ=255)
                                                                        agcGCCGGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAata                         <  2:2550479/99‑1 (MQ=255)
                                                                        aTCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAata                         >  1:464388/1‑101 (MQ=255)
                                                                             cTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcgg                    <  1:2815730/101‑1 (MQ=255)
                                                                              tGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggc                   >  1:4172325/1‑101 (MQ=255)
                                                                              tGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggc                   <  1:4254045/101‑1 (MQ=255)
                                                                               ggATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggcg                  >  2:1489842/1‑101 (MQ=255)
                                                                                gATGCTCTCGGGCCCAGAGGCGCGTTCCCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggcgg                 <  1:3734488/101‑1 (MQ=255)
                                                                                     tctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            <  2:2580312/101‑1 (MQ=255)
                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           <  1:772574/101‑1 (MQ=255)
                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           >  2:3495413/1‑101 (MQ=255)
                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           >  1:3321658/1‑101 (MQ=255)
                                                                                      caagggCCCAGGGGCGCGGTACACCGGCCGCTCACCCGGACAGGGCGAGACGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           <  2:2614508/98‑1 (MQ=255)
                                                                                         gggCCCAGAGGCGCGTTCCCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        <  1:270211/101‑1 (MQ=255)
                                                                                         gggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        <  1:818139/101‑1 (MQ=255)
                                                                                           gCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGc      >  1:1089571/1‑101 (MQ=255)
                                                                                           gCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGc      >  2:4159081/1‑101 (MQ=255)
                                                                                            cccAGAGGCGCTTTACACCGCCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGcc     <  1:3998791/101‑1 (MQ=255)
                                                                                            cccAGAGGCGCGTTCCACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGcc     <  1:2513389/101‑1 (MQ=255)
                                                                                             ccAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    <  1:1224544/101‑1 (MQ=255)
                                                                                             ccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    >  2:390519/1‑101 (MQ=255)
                                                                                             ccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    <  2:3863317/101‑1 (MQ=255)
                                                                                             ccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    <  1:609271/101‑1 (MQ=255)
                                                                                              cAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAg   <  2:2200347/101‑1 (MQ=255)
                                                                                              cAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAg   >  1:1324422/1‑101 (MQ=255)
                                                                                              cAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAg   >  1:2141795/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG