Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,459 C→G 100% G470G (GGC→GGG RCAP_rcc03391 → type I secretion system ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,4590C→G100.0% 566.3 152G470G (GGC→GGGRCAP_rcc03391type I secretion system ATPase
Reads supporting (aligned to +/- strand):  new base (68/83):  ref base (0/1):  total (68/84)

Only 100 of 175 total aligned reads displayed.

CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTC  >  NC_014034/3596361‑3596555
                                                                                                  |                                                                                                
cccgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                >  2:2912034/1‑101 (MQ=255)
cccgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                <  1:2072501/101‑1 (MQ=255)
  cgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcggc                                                                                              <  2:2412731/101‑1 (MQ=255)
  cgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcggc                                                                                              <  2:1979079/101‑1 (MQ=255)
  cgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcggc                                                                                              >  2:1206753/1‑101 (MQ=255)
    agaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcgggcc                                                                                            >  2:3799858/1‑98 (MQ=255)
    agaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGctc                                                                                            <  1:1439638/101‑1 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                          <  2:919532/101‑1 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                          <  1:367631/101‑1 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                          <  1:1013992/101‑1 (MQ=255)
        gatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgg                                                                                        <  2:253994/101‑1 (MQ=255)
        gatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgg                                                                                        <  2:4033793/101‑1 (MQ=255)
         atCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcggg                                                                                       <  1:438542/101‑1 (MQ=255)
         atCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcggg                                                                                       >  1:2662352/1‑101 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgg                                                                                    >  1:1025599/1‑101 (MQ=255)
             tcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcggg                                                                                   <  1:1414482/101‑1 (MQ=255)
              cgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGGTCTcgggcgggg                                                                                  >  2:3753472/1‑100 (MQ=255)
              cgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCgggcgggc                                                                                  <  1:4199695/101‑1 (MQ=255)
                   ccGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATc                                                                             <  1:855441/101‑1 (MQ=255)
                   ccGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATc                                                                             >  2:343351/1‑101 (MQ=255)
                    cgcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATcc                                                                            >  1:2582152/1‑101 (MQ=255)
                    cgcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATcc                                                                            <  2:370109/101‑1 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACTCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAg                                                                          <  1:3308379/101‑1 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAg                                                                          <  1:4386505/101‑1 (MQ=255)
                       gAAACGGTCGGCGGCGCATCAGCTGATCGACGATCTGCTCGAGGGATATGACACCCGGGTTTCGCTGGTGGGCGGGCGGCTCTGGGGCGGGCAGATCCAgc                                                                         <  2:3535070/101‑1 (MQ=255)
                       gAAACGGGCGGCGGGGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGATTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCacc                                                                         >  1:4420807/1‑99 (MQ=255)
                       gAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgc                                                                         >  2:27606/1‑101 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                        <  2:1010636/101‑1 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                        <  1:528347/101‑1 (MQ=255)
                         aaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcgc                                                                       >  1:4308341/1‑101 (MQ=255)
                             ggcggcggcGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCg                                                                   <  1:3955199/101‑1 (MQ=255)
                                  cggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTg                                                              <  1:1551413/101‑1 (MQ=255)
                                   ggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCTGGCAGATCCAGCGCGTCGGCCTgg                                                             <  2:1604893/101‑1 (MQ=255)
                                     cgcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCACGTTCACCGCATCAGCCTCGcc                                                           >  2:182191/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACCCCCGGGGTTCGCAGGCGGGCGGGCGACTCTCGCGCGGGGAGCTATACCGGGGCGGGCTGGccc                                                          >  2:3995086/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGGCGGCCTGGgcc                                                          >  1:936796/1‑101 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCg                                                         >  1:3968891/1‑101 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCg                                                         >  1:2179359/1‑101 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCCCTCGGGCGGGCAGATCCAGCGCGGCCGCCCGGCCCg                                                         >  2:1730734/1‑101 (MQ=255)
                                         tCAGCTGATCCTCGATCTGCCCGAGGGATATAACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCggg                                                       <  1:3683886/101‑1 (MQ=255)
                                          cAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgc                                                      >  2:3406160/1‑101 (MQ=255)
                                            gCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgctc                                                    <  1:2959475/101‑3 (MQ=255)
                                            gCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGCCGGCCCGGCCCCGgccc                                                    >  2:451335/1‑99 (MQ=255)
                                             cTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCt                                                   <  1:4132368/101‑1 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGGGTCGGCCTGGGCCGGgccgct                                                  >  2:1879174/1‑97 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                  >  2:1038585/1‑101 (MQ=255)
                                               gATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                 >  1:1897530/1‑101 (MQ=255)
                                               gATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                 <  1:451375/101‑1 (MQ=255)
                                               gATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                 >  1:4003815/1‑101 (MQ=255)
                                                aTCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCCGCGCGTCGGCCTCGGCCGGGCGGTTTa                                                >  2:2397051/1‑101 (MQ=255)
                                                aTCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGGCCGGGCGCTTTa                                                >  2:2042489/1‑101 (MQ=255)
                                                aTCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTa                                                <  1:3730286/101‑1 (MQ=255)
                                                 tCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTAc                                               >  2:3405384/1‑101 (MQ=255)
                                                  ccTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGGGGTTTACg                                              >  2:4343770/1‑101 (MQ=255)
                                                  ccTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGGCGGCCCGGGCCGGGCGCCTTTCg                                              >  2:3827831/1‑101 (MQ=255)
                                                    tCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGc                                            <  2:689056/101‑1 (MQ=255)
                                                    tCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAAATACAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGc                                            >  1:3840011/1‑101 (MQ=255)
                                                     cGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCa                                           >  2:3589212/1‑101 (MQ=255)
                                                      gATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCaa                                          >  1:3295612/1‑101 (MQ=255)
                                                      gATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCaa                                          <  2:2305393/101‑1 (MQ=255)
                                                      gATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCaa                                          >  1:2914264/1‑101 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAc                                         >  1:1853608/1‑101 (MQ=255)
                                                        tCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAcc                                        <  1:2504479/101‑1 (MQ=255)
                                                          tGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCg                                      <  1:2293086/101‑1 (MQ=255)
                                                            cccGAGGGATATGACCCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGt                                    <  2:4104627/101‑1 (MQ=255)
                                                            cccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGt                                    <  1:2554796/101‑1 (MQ=255)
                                                             ccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGGGGGCAGATCCAGCGCGGCGGCCTGGCCCGGGCGCTTTACGGCAAACCCGtg                                   >  2:1621100/1‑101 (MQ=255)
                                                             ccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtg                                   >  2:1127427/1‑101 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                 <  1:4233932/101‑1 (MQ=255)
                                                                aGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctg                                <  2:2581981/101‑1 (MQ=255)
                                                                 gggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctgc                               <  1:1676837/101‑1 (MQ=255)
                                                                 gggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctgc                               <  1:4344039/101‑1 (MQ=255)
                                                                 gggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCCGggctgc                               >  2:830475/1‑101 (MQ=255)
                                                                   gATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGctgctg                             >  1:2673007/1‑101 (MQ=255)
                                                                     tatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGt                           <  2:3839758/101‑1 (MQ=255)
                                                                      atGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTg                          <  1:694402/101‑1 (MQ=255)
                                                                      atGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGGGCTGCTgggg                          >  1:3074564/1‑99 (MQ=255)
                                                                       tGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGGGCTGCTGGCGc                         >  1:1631456/1‑101 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTc                       <  1:892376/101‑1 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTc                       >  1:2486741/1‑101 (MQ=255)
                                                                          cacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcg                      <  1:2005804/101‑1 (MQ=255)
                                                                          cacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcg                      <  1:833767/101‑1 (MQ=255)
                                                                          cacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcg                      <  1:1206846/101‑1 (MQ=255)
                                                                          cacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGGGCTcg                      >  1:782328/1‑101 (MQ=255)
                                                                            cccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgac                    <  1:3770536/101‑1 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcggcg                   >  2:3610819/1‑101 (MQ=255)
                                                                              cGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacga                  >  1:1146003/1‑101 (MQ=255)
                                                                              cGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacga                  <  1:492960/101‑1 (MQ=255)
                                                                                  tttCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCg              >  1:992771/1‑101 (MQ=255)
                                                                                  tttCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCg              >  1:3122188/1‑101 (MQ=255)
                                                                                   ttCGCAGGCGGGCGGGCGGCTCCCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCGGCTGGTGCTCGACGAGCCGa             <  2:1394739/101‑1 (MQ=255)
                                                                                    tCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGaa            <  2:321026/101‑1 (MQ=255)
                                                                                       cAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGCGCCGAATGc         >  2:600712/1‑101 (MQ=255)
                                                                                        aGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCg        >  1:3230356/1‑101 (MQ=255)
                                                                                          gcgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGaa      <  1:27619/101‑1 (MQ=255)
                                                                                          gcgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGaa      <  1:3405621/101‑1 (MQ=255)
                                                                                            gggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtc    >  2:2090384/1‑101 (MQ=255)
                                                                                            gggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtc    >  1:2047081/1‑101 (MQ=255)
                                                                                             ggcgggCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtct   <  2:1758783/101‑1 (MQ=255)
                                                                                              gcgggCGGCCCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtctc  <  2:2662180/101‑1 (MQ=255)
                                                                                                  |                                                                                                
CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTC  >  NC_014034/3596361‑3596555

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG