Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 367,286 G→A 100% A214T (GCC→ACC)  bztD → glutamate/aspartate ABC transporter ATP‑binding protein BztD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034367,2860G→A100.0% 1032.8 272A214T (GCC→ACC) bztDglutamate/aspartate ABC transporter ATP‑binding protein BztD
Reads supporting (aligned to +/- strand):  new base (144/127):  ref base (0/1):  total (144/128)

Only 100 of 272 total aligned reads displayed.

GACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCG  >  NC_014034/367190‑367383
                                                                                                |                                                                                                 
 acgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCAt                                                                                              <  2:2002659/101‑1 (MQ=255)
  cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                             <  2:2693879/101‑1 (MQ=255)
  cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                             >  1:3151295/1‑101 (MQ=255)
   gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                            >  2:137973/1‑101 (MQ=255)
     aCCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATgaga                                                                                          <  1:502389/101‑1 (MQ=255)
     aCCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATgaga                                                                                          <  2:3094416/101‑1 (MQ=255)
      cccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGAt                                                                                         <  2:2663028/101‑1 (MQ=255)
       ccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATg                                                                                        <  2:3921774/101‑1 (MQ=255)
       ccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATg                                                                                        <  1:991920/101‑1 (MQ=255)
        cACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATgg                                                                                       <  1:1801502/101‑1 (MQ=255)
        cACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATgg                                                                                       >  1:2352654/1‑101 (MQ=255)
         aCCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATggg                                                                                      <  1:4082610/101‑1 (MQ=255)
            tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCtt                                                                                   <  1:4348885/101‑1 (MQ=255)
            tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCtt                                                                                   >  2:3533422/1‑101 (MQ=255)
             cGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCttt                                                                                  <  1:3780806/101‑1 (MQ=255)
              ggCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTg                                                                                 <  2:1604227/101‑1 (MQ=255)
                ggcTTGGCCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGcc                                                                               <  2:416598/100‑1 (MQ=255)
                cgcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGcc                                                                               >  1:2668427/1‑101 (MQ=255)
                cgcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGcc                                                                               >  1:3391222/1‑101 (MQ=255)
                 gcTTGACCCCGAGATGATCAAGGAAGTGCTGGAGACGCTGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGccc                                                                              <  1:3815971/101‑1 (MQ=255)
                  cTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCa                                                                             <  1:767626/101‑1 (MQ=255)
                    tGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAgg                                                                           >  1:2466031/1‑101 (MQ=255)
                     gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGc                                                                          >  1:3091900/1‑101 (MQ=255)
                     gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGAGGGGCTTTGCCCAGGc                                                                          >  2:2042302/1‑101 (MQ=255)
                        cccGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGt                                                                       >  1:885391/1‑101 (MQ=255)
                         ccGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTg                                                                      <  1:3453167/101‑1 (MQ=255)
                          cGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCCTCACCCATGAGATGGGCTTTGCCCAGGCGGTgg                                                                     >  1:3873663/1‑101 (MQ=255)
                           ctgatgagCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGc                                                                    >  2:1730393/3‑101 (MQ=255)
                            agaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCg                                                                   >  2:1492082/1‑101 (MQ=255)
                              atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGaa                                                                 >  1:2309478/1‑101 (MQ=255)
                               tgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAc                                                                >  1:902765/1‑101 (MQ=255)
                                gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAcc                                                               <  2:1313382/101‑1 (MQ=255)
                                gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAcc                                                               <  2:4018338/101‑1 (MQ=255)
                                gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAcc                                                               <  1:4309728/101‑1 (MQ=255)
                                  tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                             >  1:3306178/1‑101 (MQ=255)
                                    aaGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgggg                                                           >  2:539271/1‑100 (MQ=255)
                                     aGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTg                                                          <  1:1710246/101‑1 (MQ=255)
                                     aGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCAAACCggggg                                                          >  2:3758941/1‑99 (MQ=255)
                                       gAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGAt                                                        >  1:613246/1‑101 (MQ=255)
                                       gAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGGGGCGAACCGGGTGAt                                                        >  2:2166659/1‑101 (MQ=255)
                                          gTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCtt                                                     <  1:3023433/101‑1 (MQ=255)
                                          gTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCtt                                                     >  1:4056322/1‑101 (MQ=255)
                                           tGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTc                                                    >  1:423435/1‑101 (MQ=255)
                                            gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                   <  2:3452290/101‑1 (MQ=255)
                                             cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                  <  1:4392889/101‑1 (MQ=255)
                                                gATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGc                                               >  2:623885/1‑101 (MQ=255)
                                                gATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGc                                               <  2:2410413/101‑1 (MQ=255)
                                                gATACGATGATCCAGCTCGCCGAGGAAGGCAAGACGATGGTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGAGAACCGGGTGAACTTCATGGc                                               >  1:1433268/1‑101 (MQ=25)
                                                 aTACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGcc                                              <  1:4282655/101‑1 (MQ=255)
                                                    cGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGCTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGAc                                           >  2:739332/1‑101 (MQ=255)
                                                      atgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACgg                                         >  1:167671/1‑101 (MQ=255)
                                                       tgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACggg                                        >  1:2811024/1‑101 (MQ=255)
                                                            cAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAt                                   <  2:4068998/101‑1 (MQ=255)
                                                            cAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAt                                   >  1:3544816/1‑101 (MQ=255)
                                                            cAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAt                                   <  1:3213792/101‑1 (MQ=255)
                                                             aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  <  2:4360115/101‑1 (MQ=255)
                                                             aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  <  2:523472/101‑1 (MQ=255)
                                                             aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  <  1:3998676/101‑1 (MQ=255)
                                                              gCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcg                                 >  2:3271479/1‑101 (MQ=255)
                                                                tCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtc                               <  2:3390985/101‑1 (MQ=255)
                                                                tCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtc                               >  2:2991338/1‑101 (MQ=255)
                                                                 cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                              >  1:3608108/1‑101 (MQ=255)
                                                                 cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTCCATGGCCGACGGGCAGAtcgtcg                              >  1:3648064/1‑101 (MQ=255)
                                                                  gCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGa                             <  2:3929987/101‑1 (MQ=255)
                                                                   ccGAGGAAGGCATGACGATGGTCGGAGTCACCTATGAGATGGGGTTTGCCCAGGCGGTGGCGAACCGGGCGATCTTCATGGCCGGCGGGCGGAtgcgggag                            >  2:1905229/1‑94 (MQ=255)
                                                                    cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                           <  2:2768323/101‑1 (MQ=255)
                                                                    cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                           <  2:1054016/101‑1 (MQ=255)
                                                                    cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                           >  1:3432064/1‑101 (MQ=255)
                                                                    cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                           >  2:1141412/1‑101 (MQ=255)
                                                                     gAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCa                          >  2:3760668/1‑101 (MQ=255)
                                                                     gAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCa                          >  2:1700294/1‑101 (MQ=255)
                                                                     gAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCa                          >  1:1246273/1‑101 (MQ=255)
                                                                      aGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                         >  1:2174177/1‑101 (MQ=255)
                                                                       ggAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGa                        <  1:1730504/101‑1 (MQ=255)
                                                                        gAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaa                       <  1:375965/101‑1 (MQ=255)
                                                                         aaGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaac                      <  1:110670/101‑1 (MQ=255)
                                                                         aaGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaac                      >  2:3894814/1‑101 (MQ=255)
                                                                          aGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     <  2:782682/101‑1 (MQ=255)
                                                                          aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     >  1:2687736/1‑101 (MQ=255)
                                                                          aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     <  1:2088533/101‑1 (MQ=255)
                                                                          aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     <  2:2227006/101‑1 (MQ=255)
                                                                            gCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaacaac                   >  2:3081478/1‑101 (MQ=255)
                                                                             cATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAcc                  >  1:2268727/1‑101 (MQ=255)
                                                                             cATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAcc                  >  2:1223675/1‑101 (MQ=255)
                                                                              aTGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                 <  2:878171/101‑1 (MQ=255)
                                                                                gACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGc               >  1:2371079/1‑101 (MQ=255)
                                                                                 aCGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCa              <  1:532601/101‑1 (MQ=255)
                                                                                 aCGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCa              >  1:1333556/1‑101 (MQ=255)
                                                                                  cGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCAt             <  2:3137994/101‑1 (MQ=255)
                                                                                  cGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCAt             >  2:3943386/1‑101 (MQ=255)
                                                                                    aTGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa           >  2:925991/1‑101 (MQ=255)
                                                                                       ctctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActt        <  1:1064418/101‑1 (MQ=255)
                                                                                       ctctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActt        >  1:1024987/1‑101 (MQ=255)
                                                                                         ctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtct      >  2:2472208/1‑101 (MQ=255)
                                                                                         ctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtct      <  2:2309328/101‑1 (MQ=255)
                                                                                         ctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtct      >  1:3638872/1‑101 (MQ=255)
                                                                                         ctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtct      <  1:2999122/101‑1 (MQ=255)
                                                                                          tGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtctt     >  1:1996801/1‑101 (MQ=255)
                                                                                             gTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcca  <  2:4397817/101‑2 (MQ=255)
                                                                                             gTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcca  <  2:167650/101‑2 (MQ=255)
                                                                                                |                                                                                                 
GACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCG  >  NC_014034/367190‑367383

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG