Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,425 T→G 4.1% intergenic (+28/+120) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4250T→G 4.1% 9.0 ‑2.3 ‑0.0171intergenic (+28/+120)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (59/104):  total (64/107)
Fisher's exact test strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.04e-01

Only 100 of 181 total aligned reads displayed.

ATGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGC  >  NC_014034/432328‑432520
                                                                                                  |                                                                                                 
agggACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.cct                                                                                               <  2:1847448/99‑3 (MQ=255)
 tGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.cctg                                                                                              <  2:1569581/101‑4 (MQ=255)
 tGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.cctg                                                                                              <  1:2080528/101‑4 (MQ=255)
  ggACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.cctgc                                                                                             >  1:3314792/1‑97 (MQ=255)
  ggACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.cctgc                                                                                             <  1:624619/101‑5 (MQ=255)
   gACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.cctgcg                                                                                            <  1:4369590/101‑6 (MQ=255)
     cccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGa                                                                                          <  2:203574/101‑1 (MQ=255)
      ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGAc                                                                                         <  2:4337221/101‑1 (MQ=255)
       gcagcgcTGGGCACGATGGGGATAGATCCGCGCACGCTTTCGGCGGCGGCGGGCCAAGACTCAGGCCGGGCAGGAGAGAGCTGACCCCCGCC.C.TGCGGACt                                                                                        >  2:726375/4‑101 (MQ=255)
       cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACt                                                                                        >  1:1397944/1‑101 (MQ=255)
        gggCGCGGGGCACGAGGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCTGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTc                                                                                       <  2:10693/101‑1 (MQ=37)
         ggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTcc                                                                                      <  1:2156478/101‑1 (MQ=255)
         ggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTcc                                                                                      <  1:3893579/101‑1 (MQ=255)
           cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTcccc                                                                                    >  2:1490750/1‑101 (MQ=255)
           cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTcccc                                                                                    <  1:2240108/101‑1 (MQ=255)
            gatGGGCACGATGGGGAGCGATCCGCGCACGCTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTccccc                                                                                   <  2:3861814/99‑1 (MQ=37)
              tGGGCACGATGGGGATCGATCCGCGCCCGCTTTCGGCGGGGGCGGGCCACGCCTGAGGCCGGGCGGGGGGGGGCGGCCCCCCG.TCC.TGCGGACTCCCCCCg                                                                                 >  2:3587263/1‑101 (MQ=37)
              tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCg                                                                                 >  2:2162566/1‑101 (MQ=255)
              tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCg                                                                                 <  2:3090481/101‑1 (MQ=255)
              tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCg                                                                                 <  1:1265373/101‑1 (MQ=255)
              tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCg                                                                                 >  2:315115/1‑101 (MQ=255)
                 gCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGa                                                                              >  2:3309019/1‑101 (MQ=255)
                  cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGat                                                                             <  2:3438372/101‑1 (MQ=255)
                   aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGata                                                                            <  1:558876/101‑1 (MQ=255)
                   aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCCGGgta                                                                            >  2:793445/1‑101 (MQ=37)
                    cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGatat                                                                           <  1:1729243/101‑1 (MQ=255)
                    cGAGGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGatat                                                                           <  1:1642365/101‑1 (MQ=255)
                     gATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATAtt                                                                          <  2:303280/101‑1 (MQ=255)
                     gATGGGGATCGATCCGCGCACGCTTTCGGCGCCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCcggggatt                                                                          >  1:3955311/1‑96 (MQ=25)
                      aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATAttt                                                                         <  2:800361/101‑1 (MQ=255)
                      aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCCGGATAttt                                                                         >  1:1944229/1‑101 (MQ=255)
                        ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCCGGGTAtttca                                                                       >  1:863520/1‑99 (MQ=25)
                           gatcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGc                                                                    <  1:1030510/101‑1 (MQ=255)
                            atcgatcCGCGCACGCTTTCGGCGGGGGCGGGCCACGCCTTAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGGCTCCCCCCGGGATATTTGAGGCa                                                                   <  1:351498/101‑1 (MQ=25)
                            atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCa                                                                   <  1:3332201/101‑1 (MQ=255)
                             tcgatcCGCGCACGCTTTCGGCGGCGGCGGNCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCaa                                                                  <  1:1170312/101‑1 (MQ=255)
                              cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                 <  1:1832292/101‑1 (MQ=255)
                              cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                 <  1:2344389/101‑1 (MQ=255)
                              cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                 <  1:1530272/101‑1 (MQ=255)
                               gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                >  1:1267948/1‑101 (MQ=255)
                               gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                <  1:4144742/101‑1 (MQ=255)
                               gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                >  1:481258/1‑101 (MQ=255)
                                atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGAt                                                               <  2:3698868/101‑1 (MQ=255)
                                 tnNGCNNACGCTTTCGGNGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGTCAAGATg                                                              <  2:1127254/101‑1 (MQ=21)
                                    gcgcACTCTTTCGGCGGCGGGGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                           <  1:1102042/101‑1 (MQ=18)
                                    gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                           >  1:2826810/1‑101 (MQ=255)
                                     cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                          <  1:4073935/101‑1 (MQ=255)
                                        aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                       <  1:3722350/101‑1 (MQ=255)
                                           cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                    <  2:2507141/101‑1 (MQ=255)
                                             ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                  <  2:1370921/101‑1 (MQ=255)
                                                ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                               >  1:2776396/1‑101 (MQ=255)
                                                  cgtcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                             <  2:2571579/98‑1 (MQ=255)
                                                  cggcggcggGCCACGCCTGAGGCCGGCAAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                             <  2:1710840/101‑1 (MQ=21)
                                                   ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                            >  2:2595712/1‑101 (MQ=255)
                                                   ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                            <  2:2773287/101‑1 (MQ=255)
                                                   ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                            <  1:1214384/101‑1 (MQ=255)
                                                   ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCCGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTc                                            >  2:3170009/1‑101 (MQ=21)
                                                         gggCCACGCCTGAGGCCGGGCAGCGGGGCGCTGCCCCCCG.TCC.TGCGAACTCCCCACGGGATATTTGAGGCAAGATGAGAGCAGATCCGGGCTTCGTTGCa                                      >  2:736359/1‑101 (MQ=18)
                                                           gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                    <  1:1879306/101‑1 (MQ=255)
                                                           gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                    <  2:2054554/101‑1 (MQ=255)
                                                             cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                  <  2:1778673/101‑1 (MQ=255)
                                                             cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                  >  1:2659957/1‑101 (MQ=255)
                                                              aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                 <  1:1916388/101‑1 (MQ=255)
                                                               cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                <  1:2370166/101‑1 (MQ=255)
                                                                gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                               >  2:4364611/1‑101 (MQ=255)
                                                                gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                               >  2:3974588/1‑101 (MQ=255)
                                                                  cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                             >  1:3680842/1‑101 (MQ=21)
                                                                  cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                             <  1:2609731/101‑1 (MQ=255)
                                                                  cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                             <  1:719163/101‑1 (MQ=255)
                                                                      ggCCGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                         <  2:2979145/101‑1 (MQ=255)
                                                                        ccGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                       <  2:2311039/101‑1 (MQ=255)
                                                                         cGGGCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                      <  2:3175109/101‑1 (MQ=255)
                                                                          gggCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                     <  1:2715247/101‑1 (MQ=255)
                                                                           ggacggggggCTCTGCCCCCCG.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt         >  2:938660/5‑101 (MQ=2)
                                                                            gCAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                   <  1:2000895/101‑1 (MQ=255)
                                                                             cAGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                  >  1:3553983/1‑101 (MQ=25)
                                                                             acggggggCTCTGCCCCCCG.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg       >  2:3465243/3‑99 (MQ=2)
                                                                              cggggggCTCTGCCCCCCG.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc      >  2:947626/2‑98 (MQ=2)
                                                                              cggggggCTCTGCCCCCCG.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc      <  1:2878129/100‑4 (MQ=2)
                                                                              aGGGGGGCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                 <  2:1915454/101‑1 (MQ=255)
                                                                               ggggggCTCTGCCCCCCG.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggcc     <  1:1418386/101‑5 (MQ=2)
                                                                               ggggggCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                >  1:1315167/1‑101 (MQ=255)
                                                                               ggggggCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                <  2:458048/101‑1 (MQ=255)
                                                                                gggggCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGCGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg               >  2:3663196/1‑101 (MQ=255)
                                                                                 ggggCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt              <  1:3258087/101‑1 (MQ=255)
                                                                                 ggggCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt              <  2:1267855/101‑1 (MQ=255)
                                                                                  gggCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg             <  2:593968/101‑1 (MQ=255)
                                                                                   ggCGCTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa            <  2:1963676/101‑1 (MQ=255)
                                                                                   ggCGCTGCCCCCCG.T.CTTGCGGCCGCCCCCCGGCATATTTGAGGCAGGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa            <  2:4257360/101‑1 (MQ=25)
                                                                                    gcgcTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa           <  1:4038635/101‑1 (MQ=255)
                                                                                     cgcTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATGCCGGGGGCGAAt          >  2:645146/1‑101 (MQ=25)
                                                                                     cgcTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt          >  1:337882/1‑101 (MQ=255)
                                                                                     cgcTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt          >  2:127379/1‑101 (MQ=255)
                                                                                     cgcTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt          <  2:1397837/101‑1 (MQ=255)
                                                                                       cTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCCGGGGGGAAttt        >  2:36233/1‑101 (MQ=25)
                                                                                       cTGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt        <  2:307592/101‑1 (MQ=255)
                                                                                        tGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCTTCTTGCCCCAAATATCCCGGGGGGGAAtttt       >  1:4268833/1‑101 (MQ=25)
                                                                                        tGCCCCCCG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt       >  1:2809354/1‑101 (MQ=255)
                                                                                            ccccG.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcg   <  1:1370521/101‑1 (MQ=255)
                                                                                             cccG.TCC.TGCGGACTCCCCCCGGGCTATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcgc  >  2:3342972/1‑101 (MQ=255)
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ATGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGC  >  NC_014034/432328‑432520

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG