Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,428 G→T 3.7% intergenic (+31/+117) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4280G→T 3.7% 8.1 ‑2.2 ‑0.2174intergenic (+31/+117)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (64/103):  total (69/105)
Fisher's exact test strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.24e-01

Only 100 of 186 total aligned reads displayed.

CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAA  >  NC_014034/432333‑432528
                                                                                                   |                                                                                                    
cccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGa                                                                                                  <  2:203574/101‑1 (MQ=255)
 ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGAc                                                                                                 <  1:3289210/101‑1 (MQ=255)
 ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGAc                                                                                                 <  2:4337221/101‑1 (MQ=255)
  gcagcgcTGGGCACGATGGGGATAGATCCGCGCACGCTTTCGGCGGCGGCGGGCCAAGACTCAGGCCGGGCAGGAGAGAGCTGACCCCC.GCC.C.TGCGGACt                                                                                                >  2:726375/4‑101 (MQ=255)
   gggCGCGGGGCACGAGGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCTGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTc                                                                                               <  2:10693/101‑1 (MQ=37)
      cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTcccc                                                                                            >  2:1490750/1‑101 (MQ=255)
       gatGGGCACGATGGGGAGCGATCCGCGCACGCTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTccccc                                                                                           <  2:3861814/99‑1 (MQ=37)
         tGGGCACGATGGGGATCGATCCGCGCCCGCTTTCGGCGGGGGCGGGCCACGCCTGAGGCCGGGCGGGGGGGGGCGGCCCCCC.G.TCC.TGCGGACTCCCCCCg                                                                                         >  2:3587263/1‑101 (MQ=37)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTccccccc                                                                                         >  2:2246242/1‑100 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCg                                                                                         >  2:315115/1‑101 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCg                                                                                         >  1:3674532/1‑101 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCg                                                                                         <  1:4064384/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCg                                                                                         <  2:3090481/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCg                                                                                         <  1:1265373/101‑1 (MQ=255)
          gggCACGATGGGGATCGATCCGCGCACGCTTTCGGAGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCgg                                                                                        <  1:2234599/101‑1 (MQ=255)
            gCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGa                                                                                      >  2:3309019/1‑101 (MQ=255)
            gCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGa                                                                                      <  1:141001/101‑1 (MQ=255)
             cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGat                                                                                     <  2:3438372/101‑1 (MQ=255)
              aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGata                                                                                    <  1:558876/101‑1 (MQ=255)
               cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGatat                                                                                   <  1:1729243/101‑1 (MQ=255)
               cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGatat                                                                                   <  1:1349423/101‑1 (MQ=255)
               cGAGGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGatat                                                                                   <  1:1642365/101‑1 (MQ=255)
                gATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATAtt                                                                                  <  2:303280/101‑1 (MQ=255)
                 aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCCGGATAttt                                                                                 >  1:1944229/1‑101 (MQ=255)
                  tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTg                                                                                <  1:262315/101‑1 (MQ=255)
                   ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCCGGGTAtttca                                                                               >  1:863520/1‑99 (MQ=25)
                    gggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAg                                                                              >  2:478899/1‑101 (MQ=255)
                      gatcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGc                                                                            <  1:1030510/101‑1 (MQ=255)
                        tcgatcCGCGCACGCTTTCGGCGGCGGCGGNCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCaa                                                                          <  1:1170312/101‑1 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                         <  1:1832292/101‑1 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                        >  1:1267948/1‑101 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                        >  1:481258/1‑101 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                        <  1:4144742/101‑1 (MQ=255)
                            tnNGCNNACGCTTTCGGNGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGTCAAGATg                                                                      <  2:1127254/101‑1 (MQ=21)
                              cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGACATTTTCGGGCAGATGaa                                                                    >  2:857126/1‑101 (MQ=21)
                               gcgcACTCTTTCGGCGGCGGGGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                   <  1:1102042/101‑1 (MQ=18)
                               gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                   >  1:2826810/1‑101 (MQ=255)
                                 gcACGCTTTCGGCGGCGGCGGGCCACGCCGGAGGCCGGGCAGGGGGGCGCGGCCCCCCGG.C.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                                 <  1:2678140/101‑1 (MQ=21)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                <  2:686013/101‑1 (MQ=255)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                <  1:354675/101‑1 (MQ=255)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                <  1:4299844/101‑1 (MQ=255)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCCGGATATTTTAGGCAAGATGAAAGcc                                                                >  2:1331927/1‑101 (MQ=21)
                                   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                               <  2:2059060/101‑1 (MQ=255)
                                   aCGCCTCCGGCGGCGGCGGGCCGCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                               <  1:3353045/101‑1 (MQ=18)
                                      cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                            <  2:2507141/101‑1 (MQ=255)
                                       tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCTCCGGGGTATTTGAGGCAAGATGAAGCCCGATcc                                                           >  1:3850092/1‑101 (MQ=21)
                                            gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                      <  2:941398/101‑1 (MQ=255)
                                             cgtcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                     <  2:2571579/98‑1 (MQ=255)
                                             cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                     <  1:2731068/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                    <  1:1214384/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                    <  2:2773287/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                    <  2:1791387/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCCGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTc                                                    >  2:3170009/1‑101 (MQ=21)
                                                  gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                <  1:3136909/99‑1 (MQ=255)
                                                   cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGGTTc                                               >  2:4038378/1‑101 (MQ=255)
                                                     ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                             >  2:3436232/1‑101 (MQ=255)
                                                      gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                            <  1:1879306/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                          <  2:173876/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                          >  2:3922964/1‑101 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTCGTTTCATcct                                          >  2:797111/1‑99 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCAGATCCGGGCTTCGTTTTACCtt                                          >  2:59682/1‑101 (MQ=18)
                                                          cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                        <  1:2370166/101‑1 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                       <  1:2575464/101‑1 (MQ=255)
                                                             cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                     >  1:3680842/1‑101 (MQ=21)
                                                             cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                     <  1:719163/101‑1 (MQ=255)
                                                                 ggCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                 <  2:2979145/101‑1 (MQ=255)
                                                                   ccGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                               <  2:2311039/101‑1 (MQ=255)
                                                                    cGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                              <  2:3175109/101‑1 (MQ=255)
                                                                      ggacggggtgCTCTGCCCCCC.G.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                 <  2:1564473/97‑1 (MQ=2)
                                                                      ggacggggggCTCTGCCCCCC.G.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                 >  1:1895554/5‑101 (MQ=2)
                                                                       gacggggggCTCTGCCCCCC.G.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttg                >  1:3903003/4‑100 (MQ=2)
                                                                       gCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                           <  1:2000895/101‑1 (MQ=255)
                                                                       gCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                           <  1:1391283/101‑1 (MQ=255)
                                                                        acggggggCTCTGCCCCCC.G.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg               >  2:3465243/3‑99 (MQ=2)
                                                                         cggggggCTCTGCCCCCC.G.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc              <  1:2878129/100‑4 (MQ=2)
                                                                         aGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                         >  1:2153747/1‑101 (MQ=255)
                                                                         aGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                         <  2:1915454/101‑1 (MQ=255)
                                                                           gggggCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGCGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg                       >  2:3663196/1‑101 (MQ=255)
                                                                            ggggCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                      <  2:1267855/101‑1 (MQ=255)
                                                                             gggCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                     <  1:3436471/101‑1 (MQ=255)
                                                                              ggCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa                    <  2:1963676/101‑1 (MQ=255)
                                                                              ggCGCTGCCCCCC.G.T.CTTGCGGCCGCCCCCCGGCATATTTGAGGCAGGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa                    <  2:4257360/101‑1 (MQ=25)
                                                                                cgcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATGCCGGGGGCGAAt                  >  2:645146/1‑101 (MQ=25)
                                                                                cgcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                  <  2:1397837/101‑1 (MQ=255)
                                                                                  cTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCCGGGGGGAAttt                >  2:36233/1‑101 (MQ=25)
                                                                                  cTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                >  1:4091887/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt               <  1:1490855/101‑1 (MQ=255)
                                                                                   tGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt               >  1:2809354/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt               >  2:748745/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGACTTCGTTTCATCTTGCCCCAAATATCCCCGGGGTGAAtttt               >  2:4412183/1‑101 (MQ=25)
                                                                                   cgCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt               <  1:2284194/100‑1 (MQ=255)
                                                                                    gCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg              >  2:3067673/1‑101 (MQ=255)
                                                                                           ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa         <  2:2659785/96‑1 (MQ=255)
                                                                                            cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg        <  1:3467986/97‑1 (MQ=255)
                                                                                            cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg        >  1:455790/5‑101 (MQ=255)
                                                                                            cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATACGGGCCTCGTTTCATTTTGCCCCAAATATCCCGGGGGGGGATTTTGGAGCAg        >  1:3947264/5‑101 (MQ=18)
                                                                                              tcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc      <  1:4015698/99‑1 (MQ=255)
                                                                                                c.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa    >  2:3699599/1‑101 (MQ=255)
                                                                                                  tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGACTTTTGGCGCAGCCaaa   >  2:1777588/1‑101 (MQ=255)
                                                                                                  tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa   >  1:1386625/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAA  >  NC_014034/432333‑432528

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG