Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,479 G→A 4.8% intergenic (+82/+66) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4790G→A 4.8% 12.8 ‑0.7 ‑0.2167intergenic (+82/+66)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (78/81):  total (83/84)
Fisher's exact test strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.65e-01

Only 100 of 186 total aligned reads displayed.

GCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGT  >  NC_014034/432380‑432579
                                                                                                     |                                                                                                    
gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                                      <  2:1134490/101‑1 (MQ=255)
  ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                                    <  1:2047272/101‑1 (MQ=255)
   gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                                   <  1:3136909/99‑1 (MQ=255)
    cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGGTTc                                                                                                  >  2:4038378/1‑101 (MQ=255)
       gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                               <  2:2054554/101‑1 (MQ=255)
       gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                               <  1:1879306/101‑1 (MQ=255)
        ccACGCCTGAGGCCGGGGAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                              <  1:1267872/101‑1 (MQ=255)
         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                             <  2:1778673/101‑1 (MQ=255)
         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                             <  2:173876/101‑1 (MQ=255)
         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                             >  1:2659957/1‑101 (MQ=255)
         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCAGATCCGGGCTTCGTTTTACCtt                                                                                             >  2:59682/1‑101 (MQ=18)
          aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                            <  1:1916388/101‑1 (MQ=255)
           cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                           <  2:3170758/101‑1 (MQ=255)
           cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                           >  1:558702/1‑101 (MQ=255)
            gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                          >  2:4364611/1‑101 (MQ=255)
            gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                          >  2:3974588/1‑101 (MQ=255)
              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                        >  1:3680842/1‑101 (MQ=21)
              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                        <  1:719163/101‑1 (MQ=255)
              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                        <  1:2609731/101‑1 (MQ=255)
               tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                                       <  1:599427/101‑1 (MQ=255)
                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                                    <  2:2979145/101‑1 (MQ=255)
                    ccGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                                  <  2:2311039/101‑1 (MQ=255)
                     cGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                                 <  2:3175109/101‑1 (MQ=255)
                      gggCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                                <  1:2715247/101‑1 (MQ=255)
                       ggacggggtgCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                    <  2:1564473/97‑1 (MQ=2)
                       ggacggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                    >  2:938660/5‑101 (MQ=2)
                        gacggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttg                                                                   >  1:3903003/4‑100 (MQ=2)
                        gCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                              <  1:1391283/101‑1 (MQ=255)
                         cAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                                                                             >  1:3553983/1‑101 (MQ=25)
                         acggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                                                                  >  2:3465243/3‑99 (MQ=2)
                          cggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                 <  1:2878129/100‑4 (MQ=2)
                          aGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                            <  2:1915454/101‑1 (MQ=255)
                           ggggggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                           <  2:458048/101‑1 (MQ=255)
                           ggggggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                           <  1:1660942/101‑1 (MQ=255)
                               ggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                       <  2:1963676/101‑1 (MQ=255)
                               ggCGCTGCCCCCCGT.CTTGCGGCCGCCCCCCGGCATATTTGAGGCAGGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                       <  2:4257360/101‑1 (MQ=25)
                                gcccTGCCCCCCGTCC.TGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                                      <  2:2566367/98‑1 (MQ=25)
                                 cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                                     >  2:127379/1‑101 (MQ=255)
                                 cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                                     <  2:1397837/101‑1 (MQ=255)
                                 cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                                     >  1:337882/1‑101 (MQ=255)
                                   cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCCGGGGGGAAttt                                                                   >  2:36233/1‑101 (MQ=25)
                                   cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                   >  1:4091887/1‑101 (MQ=255)
                                    tGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                  <  1:1490855/101‑1 (MQ=255)
                                    tGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                  >  2:748745/1‑101 (MQ=255)
                                     gCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                 >  2:3455892/1‑101 (MQ=255)
                                     gCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                 >  2:3067673/1‑101 (MQ=255)
                                        ccccGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcg                                                              <  1:1370521/101‑1 (MQ=255)
                                         cccGTCC.TGCGGACTCCCCCCGGGCTATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcgc                                                             >  2:3342972/1‑101 (MQ=255)
                                          ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa                                                            <  2:2659785/96‑1 (MQ=255)
                                          ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa                                                            <  2:667298/96‑1 (MQ=255)
                                           cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                           >  1:455790/5‑101 (MQ=255)
                                           cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATACGGGCCTCGTTTCATTTTGCCCCAAATATCCCGGGGGGGGATTTTGGAGCAg                                                           >  1:3947264/5‑101 (MQ=18)
                                             tcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                                         <  1:4015698/99‑1 (MQ=255)
                                              cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                        >  1:3461589/2‑101 (MQ=255)
                                               c.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                       <  2:723720/101‑1 (MQ=255)
                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                    <  1:2162705/101‑1 (MQ=255)
                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                    <  2:3966551/101‑1 (MQ=255)
                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                                   <  1:2020531/101‑1 (MQ=255)
                                                       cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                                                <  2:3314560/101‑1 (MQ=255)
                                                        tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGTCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                               <  2:3680589/101‑1 (MQ=255)
                                                        tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                               >  1:747322/1‑101 (MQ=255)
                                                          cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                             <  2:3119292/101‑1 (MQ=255)
                                                          cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                             >  1:951457/1‑101 (MQ=255)
                                                           ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                            <  1:4364882/101‑1 (MQ=255)
                                                            cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                           <  2:2776206/101‑1 (MQ=255)
                                                             ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGACAgc                                          >  1:3726286/1‑101 (MQ=255)
                                                              cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                         >  2:1493328/1‑101 (MQ=255)
                                                              cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                         <  1:3640770/101‑1 (MQ=255)
                                                               gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                        >  2:1119944/1‑101 (MQ=255)
                                                                 gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                      >  2:1874104/1‑101 (MQ=255)
                                                                   tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                    <  2:3763654/101‑1 (MQ=255)
                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                   >  2:4278323/1‑101 (MQ=255)
                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                   >  1:3175898/1‑101 (MQ=255)
                                                                     tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                  >  1:2130728/1‑101 (MQ=255)
                                                                     tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                  >  1:2401888/1‑101 (MQ=255)
                                                                      ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                 <  1:3309251/101‑1 (MQ=255)
                                                                       tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                                >  1:3586026/1‑101 (MQ=255)
                                                                         aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                              >  1:3760642/1‑101 (MQ=255)
                                                                           gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                            <  2:2077529/101‑1 (MQ=255)
                                                                              aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                         >  1:150635/1‑101 (MQ=255)
                                                                               gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc                        <  1:769328/101‑1 (MQ=255)
                                                                                 tGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc                      >  2:1599185/1‑101 (MQ=255)
                                                                                    aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                   <  1:1050148/101‑1 (MQ=255)
                                                                                    aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                   >  2:2734530/1‑101 (MQ=255)
                                                                                       ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc                <  2:1123079/101‑1 (MQ=255)
                                                                                       ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc                <  2:863435/101‑1 (MQ=255)
                                                                                       ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc                >  1:2308350/1‑101 (MQ=255)
                                                                                        cGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg               >  1:1897183/1‑101 (MQ=255)
                                                                                         gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc              >  1:2813568/1‑101 (MQ=255)
                                                                                          aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg             <  1:2246387/101‑1 (MQ=255)
                                                                                          aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg             <  1:3000960/101‑1 (MQ=255)
                                                                                           tCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGcggcgcggcggcgcgg            >  2:4122463/1‑101 (MQ=255)
                                                                                             cGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg          >  1:143536/1‑101 (MQ=255)
                                                                                              gggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc         >  2:1348244/1‑101 (MQ=255)
                                                                                               ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa        <  2:481206/101‑1 (MQ=255)
                                                                                                  ttCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGcc     >  2:2888728/1‑101 (MQ=255)
                                                                                                   tCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCg    >  1:1164110/1‑101 (MQ=255)
                                                                                                     gTTTCATCTTGCCCCAAATATCCCGGGGTTGATTTTTGGCGCAGCCAAGATAGCGGGGGGCGCCGCCTCTCTACGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  2:2873142/1‑101 (MQ=21)
                                                                                                     gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  1:1108083/1‑101 (MQ=255)
                                                                                                     gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  1:461794/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGT  >  NC_014034/432380‑432579

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG