Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,493 C→G 4.6% intergenic (+96/+52) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4930C→G 4.6% 10.7 ‑0.3 ‑0.0173intergenic (+96/+52)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (85/80):  total (90/83)
Fisher's exact test strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.73e-01

Only 100 of 193 total aligned reads displayed.

CTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCG  >  NC_014034/432394‑432592
                                                                                                    |                                                                                                    
cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                                      >  1:3680842/1‑101 (MQ=21)
cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                                      <  1:719163/101‑1 (MQ=255)
 tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                                                     <  1:599427/101‑1 (MQ=255)
      ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                                                <  2:2311039/101‑1 (MQ=255)
       cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                                               <  2:3175109/101‑1 (MQ=255)
        gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                                              <  1:2715247/101‑1 (MQ=255)
          gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttg                                                                                >  1:3903003/4‑100 (MQ=2)
          gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                                            <  1:1391283/101‑1 (MQ=255)
          gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                                            <  1:2000895/101‑1 (MQ=255)
           acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttgg                                                                               >  2:3465243/3‑99 (MQ=2)
            cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttggc                                                                              >  2:947626/2‑98 (MQ=2)
            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                                          >  1:2153747/1‑101 (MQ=255)
            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                                          <  2:1915454/101‑1 (MQ=255)
             ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                                         >  1:1315167/1‑101 (MQ=255)
              gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGCGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg                                                                                        >  2:3663196/1‑101 (MQ=255)
               ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGCTATTTGAGGCAATACGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCTAAATATCCCGGGGGt                                                                                       >  2:3000739/1‑101 (MQ=25)
                gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                                      <  1:3436471/101‑1 (MQ=255)
                 ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.a                                                                                    <  2:1963676/101‑1 (MQ=255)
                  gcccTGCCCCCCGTCCTGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                                                   <  2:2566367/98‑1 (MQ=25)
                   cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATGCCGGGGGCG.AAt                                                                                  >  2:645146/1‑101 (MQ=25)
                   cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                                                  >  2:127379/1‑101 (MQ=255)
                   cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                                                  <  2:1397837/101‑1 (MQ=255)
                     cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                                                <  2:307592/101‑1 (MQ=255)
                     cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                                                >  1:4091887/1‑101 (MQ=255)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCTTCTTGCCCCAAATATCCCGGGGGGG.AAtttt                                                                               >  1:4268833/1‑101 (MQ=25)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                                               >  1:2809354/1‑101 (MQ=255)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                                               >  2:748745/1‑101 (MQ=255)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGACTTCGTTTCATCTTGCCCCAAATATCCCCGGGGTG.AAtttt                                                                               >  2:4412183/1‑101 (MQ=25)
                       gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTg                                                                              >  2:3455892/1‑101 (MQ=255)
                         cccccGTCCTGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTTATCTTGCCCCCAATATTCCGGGGGGGAAATTTTgg                                                                             >  2:3130228/1‑101 (MQ=21)
                          ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGgcg                                                                           <  1:1370521/101‑1 (MQ=255)
                            ccgtccTGCGGACTCCCCCCGGGATATTTTAGGCAAAATGAAAACCGAACCGGGGTTCGTTTCATCTTGCCCCCAAAATCCCCGGGGGG.AATTTTgtggcc                                                                         >  2:1539672/6‑96 (MQ=18)
                            ccgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCa                                                                         <  2:2659785/96‑1 (MQ=255)
                             cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAg                                                                        <  1:3467986/97‑1 (MQ=255)
                             cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATACGGGCCTCGTTTCATTTTGCCCCAAATATCCCGGGGGGG.GATTTTGGAGCAg                                                                        >  1:3947264/5‑101 (MQ=18)
                              gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGTCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGc                                                                       >  2:4209462/4‑101 (MQ=255)
                               tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                                                      <  1:4379286/99‑1 (MQ=255)
                               tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                                                      <  1:4015698/99‑1 (MQ=255)
                                 cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaa                                                                    >  2:3699599/1‑101 (MQ=255)
                                  tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.ACTTTTGGCGCAGCCaaa                                                                   >  2:1777588/1‑101 (MQ=255)
                                    cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAag                                                                 <  2:3966551/101‑1 (MQ=255)
                                     ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                                                <  1:748825/101‑1 (MQ=255)
                                     ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                                                >  1:3575681/1‑101 (MQ=255)
                                        cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAggg                                                             <  2:3314560/101‑1 (MQ=255)
                                         tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                                            >  2:4361890/1‑101 (MQ=255)
                                           cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGc                                                          <  1:3645813/101‑1 (MQ=255)
                                           cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGc                                                          >  1:951457/1‑101 (MQ=255)
                                             cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAg                                                        <  2:2776206/101‑1 (MQ=255)
                                              ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                       <  1:3587511/101‑1 (MQ=255)
                                               cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                      >  2:1422484/1‑101 (MQ=255)
                                               cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAccg                                                      <  2:2949293/101‑1 (MQ=255)
                                                gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                                     >  2:1119944/1‑101 (MQ=255)
                                                  gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                   >  2:1874104/1‑101 (MQ=255)
                                                  gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                   >  2:2771542/1‑101 (MQ=255)
                                                     atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                >  1:3175898/1‑101 (MQ=255)
                                                      tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                               >  1:2130728/1‑101 (MQ=255)
                                                       ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                              <  2:1386528/101‑1 (MQ=255)
                                                       ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                              <  1:3309251/101‑1 (MQ=255)
                                                        tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCCATc                                             >  1:2845410/1‑101 (MQ=255)
                                                         gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                                            >  1:2033703/1‑101 (MQ=255)
                                                          aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                                           >  1:3760642/1‑101 (MQ=255)
                                                           ggCAAGCTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                          <  1:736439/101‑1 (MQ=255)
                                                           ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                          <  2:4268564/101‑1 (MQ=255)
                                                            gCAAGATGAAAGCCGGTCGGGGCTGCGTTTCATCTTGCCCCAAATATCCCGGGGGTT.ATTTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                         <  2:669907/101‑1 (MQ=25)
                                                            gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                         <  2:2077529/101‑1 (MQ=255)
                                                               aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                                      >  1:150635/1‑101 (MQ=255)
                                                                  tGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc                                   >  2:1599185/1‑101 (MQ=255)
                                                                   gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                  >  1:1472402/1‑101 (MQ=255)
                                                                     aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                >  2:2734530/1‑101 (MQ=255)
                                                                     aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                <  1:1050148/101‑1 (MQ=255)
                                                                     aaGCCGATCCGGGCTTCGNTTCATCTTGCCCCAACTATCCCGGGGGTN.AATTTTGGCGCAGCCAAAAGAGNNNNNCGCCCNCCCTCNNCCCcncgcgcggc                                >  1:2891020/1‑101 (MQ=21)
                                                                        ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc                             >  1:2308350/1‑101 (MQ=255)
                                                                         cGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg                            >  1:1897183/1‑101 (MQ=255)
                                                                           aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg                          <  1:3000960/101‑1 (MQ=255)
                                                                           aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg                          <  1:2246387/101‑1 (MQ=255)
                                                                              cGGGCTTCGTTTCATCTTGCCGCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg                       <  2:4085539/101‑1 (MQ=255)
                                                                                ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCATCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa                     <  2:1123386/101‑1 (MQ=255)
                                                                                ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa                     <  2:929830/101‑1 (MQ=255)
                                                                                ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa                     <  1:3130452/101‑1 (MQ=255)
                                                                                ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa                     <  2:481206/101‑1 (MQ=255)
                                                                                   tttttttCATCTGCCAACAAATACCGGGGGGATG.ATTTTGGGCGCAGCAAAAGGGGGGGGCCCCCCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGcc                  <  1:1422591/97‑1 (MQ=255)
                                                                                    tCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCg                 >  1:1164110/1‑101 (MQ=255)
                                                                                      gTTTCATCTTGCCCCAAATATCCCGGGGTTG.ATTTTTGGCGCAGCCAAGATAGCGGGGGGCGCCGCCTCTCTACGCGGCGCGGCGGCGCGGCGCAGCCGGt               >  2:2873142/1‑101 (MQ=21)
                                                                                      gTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt               >  1:1108083/1‑101 (MQ=255)
                                                                                      gTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt               >  1:461794/1‑101 (MQ=255)
                                                                                       tttCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc              >  1:588213/1‑101 (MQ=255)
                                                                                       tttCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc              <  1:4209723/101‑1 (MQ=255)
                                                                                        ttCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg             >  1:1894726/1‑101 (MQ=255)
                                                                                        ttCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg             >  2:593546/1‑101 (MQ=255)
                                                                                         tCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGt            <  2:3950925/101‑1 (MQ=255)
                                                                                         tCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGt            <  2:3575433/101‑1 (MQ=255)
                                                                                         tCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGt            >  1:2217853/1‑101 (MQ=255)
                                                                                          cATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt           <  1:889736/101‑1 (MQ=255)
                                                                                           aTCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTg          >  2:711534/1‑101 (MQ=255)
                                                                                             cTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGAt        >  1:1572023/1‑101 (MQ=255)
                                                                                              ttGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATc       <  2:1315070/101‑1 (MQ=255)
                                                                                                gCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgc     <  1:3343209/101‑1 (MQ=255)
                                                                                                gCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgc     <  1:3699852/101‑1 (MQ=255)
                                                                                                  cccAAATATCCCGGGGGTG.AATTTTAGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgc   >  1:2618460/1‑101 (MQ=255)
                                                                                                   ccAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgcg  >  1:2449409/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCG  >  NC_014034/432394‑432592

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG