Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 441,868 T→G 100% V329G (GTC→GGC)  gabD → succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034441,8680T→G100.0% 658.2 175V329G (GTC→GGC) gabDsuccinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (80/95):  ref base (0/0):  total (80/95)

Only 100 of 188 total aligned reads displayed.

CCAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGTCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGGCGGGCGGC  >  NC_014034/441770‑441965
                                                                                                   |                                                                                                 
  aGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGAc                                                                                               <  1:3714559/101‑1 (MQ=255)
  aGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGAc                                                                                               <  1:179424/101‑1 (MQ=255)
  aGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGAc                                                                                               <  1:930079/101‑1 (MQ=255)
   gACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACg                                                                                              <  2:3099823/101‑1 (MQ=255)
    aCCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGCCGGCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGAGGCGCGCCAACTCAATGTCGGCGACgg                                                                                             >  2:3470732/1‑101 (MQ=255)
     ccTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACggg                                                                                            >  2:558609/1‑101 (MQ=255)
       tGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGGGGCGGCGGGGCGCAAGCTCCATGTCGGCGGCGGGCt                                                                                          >  2:680704/1‑101 (MQ=255)
          gTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGaa                                                                                       <  1:1999908/101‑1 (MQ=255)
             tGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGa                                                                                    <  1:1081783/101‑1 (MQ=255)
             tGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGa                                                                                    <  1:3758622/101‑1 (MQ=255)
             tGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGa                                                                                    >  1:4157401/1‑101 (MQ=255)
             tGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGa                                                                                    >  1:4417803/1‑101 (MQ=255)
             tGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGGGCGCAAGCTCAATGTCGGCGACGGGCTGAAggg                                                                                    >  1:3533690/1‑100 (MQ=255)
              gcgcGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGAc                                                                                   <  1:271309/101‑1 (MQ=255)
              gcgcGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGAc                                                                                   <  1:155446/101‑1 (MQ=255)
              gcgcGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGAc                                                                                   <  2:3923796/101‑1 (MQ=255)
              gcgcGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGAc                                                                                   >  1:3215554/1‑101 (MQ=255)
               cgcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCGAGCTCAATGTCGGCGACGGGCTGAAGGACg                                                                                  >  1:2481564/1‑101 (MQ=255)
               cgcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACg                                                                                  <  1:2316413/101‑1 (MQ=255)
               cgcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACg                                                                                  <  2:1399482/101‑1 (MQ=255)
               cgcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACg                                                                                  <  2:2878561/101‑1 (MQ=255)
               cgcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGGGCGCAAGCTCAATGTCGGCGACGGGGTGGAGGaga                                                                                  >  1:1086621/1‑99 (MQ=255)
                 cgAACCGGATCTATGTGNAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGc                                                                                <  2:258428/101‑1 (MQ=255)
                  gAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCg                                                                               >  2:3195724/1‑101 (MQ=255)
                  gAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTGAGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCg                                                                               <  2:3760699/101‑1 (MQ=255)
                   aaCCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGGGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCgg                                                                              >  2:2133565/1‑100 (MQ=255)
                    aCCGGATCTATGTGCGGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTg                                                                             <  2:2358821/101‑1 (MQ=255)
                    aCCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTg                                                                             <  2:2018913/101‑1 (MQ=255)
                     ccGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGa                                                                            <  2:1604997/101‑1 (MQ=255)
                     ccGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGa                                                                            >  2:750717/1‑101 (MQ=255)
                      cGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGac                                                                           >  1:400740/1‑101 (MQ=255)
                      cGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGac                                                                           >  1:1448196/1‑101 (MQ=255)
                       ggATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGacc                                                                          <  2:991196/101‑1 (MQ=255)
                          taagggtgCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCCGAAGGACGGCGTGaccacc                                                                       >  2:4082748/6‑101 (MQ=255)
                          tCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGaccacc                                                                       <  1:2096710/101‑1 (MQ=255)
                          tCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGaccacc                                                                       <  1:2962584/101‑1 (MQ=255)
                          tCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGaccacc                                                                       >  1:2023968/1‑101 (MQ=255)
                             aTGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCggg                                                                    <  1:2276105/101‑1 (MQ=255)
                              tgtgCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCCCCGGGc                                                                   >  2:1802750/1‑101 (MQ=255)
                              tgtgCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGACCACCCGGc                                                                   >  2:4032522/1‑101 (MQ=255)
                                   aGGGGGGCGTTTATGGCCCTTTCGCGGAAAAAGCGGCGGGCGCCGGTGC.CCAGCCCCAAGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTg                                                              <  2:2105123/101‑1 (MQ=255)
                                     gcgggcgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGCGCTGAAGGACGCCGTGACGACGGGGTCCTTGAt                                                            <  2:1884326/101‑1 (MQ=255)
                                       gggcgTCTATGAGACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtct                                                          >  1:2420936/1‑101 (MQ=255)
                                       gggcgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtct                                                          >  1:425694/1‑101 (MQ=255)
                                       gggcgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtct                                                          >  2:3817197/1‑101 (MQ=255)
                                       gggcgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtct                                                          <  1:1370424/101‑1 (MQ=255)
                                        ggcgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtctc                                                         <  2:2547262/101‑1 (MQ=255)
                                         gcgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCg                                                        <  1:992608/101‑1 (MQ=255)
                                         gcgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCg                                                        >  1:2384352/1‑101 (MQ=255)
                                          cgTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCgc                                                       >  2:3504052/1‑100 (MQ=255)
                                           gTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGc                                                      >  1:1690862/1‑101 (MQ=255)
                                            tCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGcc                                                     >  1:600421/1‑101 (MQ=255)
                                            tCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGcc                                                     <  2:2970003/101‑1 (MQ=255)
                                            tCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGcc                                                     <  2:3520968/101‑1 (MQ=255)
                                            tCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGcc                                                     <  2:2462164/101‑1 (MQ=255)
                                                tGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  1:1807697/101‑1 (MQ=255)
                                                tGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 >  2:357018/1‑101 (MQ=255)
                                                tGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  1:3312741/101‑1 (MQ=255)
                                                tGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  1:3299766/101‑1 (MQ=255)
                                                tGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  2:1786015/101‑1 (MQ=255)
                                                 gACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCGCCGGGCCCTTGATCTCGGCCGATGc                                                <  1:2232476/101‑1 (MQ=255)
                                                 gACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATgg                                                >  1:2548737/1‑100 (MQ=255)
                                                 gACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGc                                                <  1:189181/101‑1 (MQ=255)
                                                  acacTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCg                                               <  2:4173028/101‑1 (MQ=255)
                                                    acTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGt                                             <  2:72740/101‑1 (MQ=255)
                                                      tttCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCg                                           >  2:881998/1‑101 (MQ=255)
                                                       ttCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCga                                          <  2:4444162/101‑1 (MQ=255)
                                                          gcgG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGaa                                       <  2:3246396/101‑1 (MQ=255)
                                                          gcgG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGaa                                       <  2:582365/101‑1 (MQ=255)
                                                           cgG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAg                                      >  2:2656145/1‑101 (MQ=255)
                                                           cgG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAg                                      <  1:303485/101‑1 (MQ=255)
                                                             g.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGt                                    <  1:1357372/101‑1 (MQ=255)
                                                               aaaaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTc                                   <  2:2711423/101‑1 (MQ=255)
                                                                  aGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGGCggg                                >  2:4270855/1‑99 (MQ=255)
                                                                    cTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGa                              >  1:2097461/1‑101 (MQ=255)
                                                                     tGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGATGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGaa                             >  2:4388594/1‑101 (MQ=255)
                                                                     tGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGaa                             <  1:3458905/101‑1 (MQ=255)
                                                                      ggcggcggcGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAAc                            >  2:455710/1‑101 (MQ=255)
                                                                         ggcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACata                         >  2:111933/1‑101 (MQ=255)
                                                                           cggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATc                       <  2:3648418/101‑1 (MQ=255)
                                                                           cggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATc                       <  1:728160/101‑1 (MQ=255)
                                                                             gcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGc                     <  1:3680030/101‑1 (MQ=255)
                                                                               ggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCg                   <  2:3443918/101‑1 (MQ=255)
                                                                                gTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGGCCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGa                  >  2:2274417/1‑101 (MQ=255)
                                                                                gTGAGCAAGATCAATGTCGGCGGCGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGACCATATCGCCGa                  >  2:168406/1‑101 (MQ=255)
                                                                                   cgcAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtgt               <  1:455750/101‑1 (MQ=255)
                                                                                    gcAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtgtg              <  1:561156/101‑1 (MQ=255)
                                                                                    gcAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtgtg              >  2:1493811/1‑101 (MQ=255)
                                                                                     cAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtgg             >  2:1222529/1‑101 (MQ=255)
                                                                                      taGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            <  2:2843808/100‑1 (MQ=255)
                                                                                      aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            >  1:3499071/1‑101 (MQ=255)
                                                                                       aGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggta           >  1:2844015/1‑100 (MQ=255)
                                                                                        gCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGtggtgg          <  2:372448/101‑1 (MQ=255)
                                                                                        gCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGtggtgg          >  2:3026456/1‑101 (MQ=255)
                                                                                         cTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggc         >  2:402950/1‑101 (MQ=255)
                                                                                         cTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggc         <  1:1306619/101‑1 (MQ=255)
                                                                                         cTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggc         >  2:1914631/1‑101 (MQ=255)
                                                                                          tCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcg        >  1:1831431/1‑101 (MQ=255)
                                                                                            aaTGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcggg      <  2:4252847/101‑1 (MQ=255)
                                                                                               gtcggcGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGgcgggcgg   <  2:4295376/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
CCAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGG.AAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGTCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGGCGGGCGGC  >  NC_014034/441770‑441965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG