Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 566,409 A→G 100% F80L (TTC→CTC)  RCAP_rcc00528 ← carboxymuconolactone decarboxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034566,4090A→G100.0% 700.2 185F80L (TTC→CTC) RCAP_rcc00528carboxymuconolactone decarboxylase
Reads supporting (aligned to +/- strand):  new base (85/100):  ref base (0/0):  total (85/100)

Only 100 of 190 total aligned reads displayed.

CTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566311‑566506
                                                                                                  |                                                                                                 
cTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGtt                                                                                                 >  1:1007433/1‑101 (MQ=255)
   ggcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCt                                                                                              <  2:2039177/101‑1 (MQ=255)
    gcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATTATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGGGCAGATGTCGGCGAGTTccgc                                                                                             >  1:4400146/1‑99 (MQ=255)
    gcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTc                                                                                             <  2:490438/101‑1 (MQ=255)
     cggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcg                                                                                            <  1:3689756/101‑1 (MQ=255)
      ggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgc                                                                                           <  2:2423306/101‑1 (MQ=255)
      ggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgc                                                                                           <  2:3823425/101‑1 (MQ=255)
       gcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcg                                                                                          <  2:1279400/101‑1 (MQ=255)
        cAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcgc                                                                                         >  1:3328140/1‑101 (MQ=255)
         aaGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcgcg                                                                                        <  2:1564118/101‑1 (MQ=255)
           gTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTg                                                                                      <  2:507858/101‑1 (MQ=255)
           gTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTg                                                                                      <  2:3327903/101‑1 (MQ=255)
             tCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgcc                                                                                    >  2:803216/1‑101 (MQ=255)
                tCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccgcc                                                                                 <  2:2594197/101‑1 (MQ=255)
                tCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCCCGCGTgccgcc                                                                                 >  1:604264/1‑101 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                >  1:1091355/1‑101 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGGGccgccc                                                                                >  2:4094705/1‑101 (MQ=255)
                  ccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccg                                                                               <  2:3873486/101‑1 (MQ=255)
                    agcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCcgcg                                                                             <  1:3894151/101‑1 (MQ=255)
                       agGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCgcgcgc                                                                          >  2:2507183/1‑101 (MQ=255)
                        ggCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGca                                                                         >  1:4035581/1‑101 (MQ=255)
                        ggCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGca                                                                         >  2:3630506/1‑101 (MQ=255)
                         gCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcat                                                                        <  1:1914275/101‑1 (MQ=255)
                         gCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCAACTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcat                                                                        <  2:3996579/101‑1 (MQ=255)
                          ccAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatc                                                                       <  2:43827/101‑1 (MQ=255)
                           cAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatca                                                                      >  1:3017279/1‑101 (MQ=255)
                             aGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCatcatc                                                                    <  2:2996311/101‑1 (MQ=255)
                              ggCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcg                                                                   <  1:3116177/101‑1 (MQ=255)
                                 aTGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCgcgc                                                                >  2:1569907/1‑101 (MQ=255)
                                  tGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGcc                                                               >  1:176038/1‑101 (MQ=255)
                                  tGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGcc                                                               <  1:4438333/101‑1 (MQ=255)
                                    ggCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGa                                                             <  2:746984/101‑1 (MQ=255)
                                     gCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAt                                                            >  1:3457755/1‑101 (MQ=255)
                                      ccGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtg                                                           <  1:3338968/101‑1 (MQ=255)
                                       cGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgt                                                          <  2:3115436/101‑1 (MQ=255)
                                       cGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgt                                                          <  1:2245519/101‑1 (MQ=255)
                                        gCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGCtgtg                                                         >  2:4297829/1‑101 (MQ=255)
                                         cATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGa                                                        >  1:3568857/1‑101 (MQ=255)
                                         cATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGa                                                        <  1:1399114/101‑1 (MQ=255)
                                         cATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGa                                                        <  2:2100576/101‑1 (MQ=255)
                                          aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       <  1:95902/101‑1 (MQ=255)
                                          aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       <  1:3217977/101‑1 (MQ=255)
                                          aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCACGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       >  1:3756029/1‑101 (MQ=255)
                                              atcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGcc                                                   <  1:4040714/101‑1 (MQ=255)
                                               tcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  <  1:2160531/101‑1 (MQ=255)
                                                catcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGa                                                 >  1:1005860/1‑101 (MQ=255)
                                                catcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGa                                                 >  2:4162256/1‑101 (MQ=255)
                                                 atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                >  1:764836/1‑101 (MQ=255)
                                                 atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                >  1:979221/1‑101 (MQ=255)
                                                 atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                <  2:3772139/101‑1 (MQ=255)
                                                  tcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCTATg                                               <  2:716844/101‑1 (MQ=255)
                                                   cGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGc                                              >  1:4178167/1‑101 (MQ=255)
                                                   cGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGc                                              <  1:145635/101‑1 (MQ=255)
                                                   cGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGc                                              >  1:2362307/1‑101 (MQ=255)
                                                    gCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCa                                             >  1:1408209/1‑101 (MQ=255)
                                                     ccGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAg                                            <  2:3469146/101‑1 (MQ=255)
                                                      cGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAgg                                           <  2:3366840/101‑1 (MQ=255)
                                                      cGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAgg                                           >  2:3102521/1‑101 (MQ=255)
                                                      cGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAgg                                           <  1:3517774/101‑1 (MQ=255)
                                                        ggCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGc                                         <  2:2282565/101‑1 (MQ=255)
                                                         gccgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCt                                        <  2:1957462/101‑1 (MQ=255)
                                                          ccgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTc                                       >  1:2730332/1‑101 (MQ=255)
                                                           cgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCg                                      >  2:341121/1‑101 (MQ=255)
                                                           cgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCg                                      >  1:2525273/1‑101 (MQ=255)
                                                           cgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCg                                      >  2:60550/1‑101 (MQ=255)
                                                             ccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGcg                                    >  1:2905739/1‑100 (MQ=255)
                                                             ccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCa                                    <  2:589443/101‑1 (MQ=255)
                                                              cgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAg                                   <  1:2746691/101‑1 (MQ=255)
                                                              cgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAg                                   <  2:1091267/101‑1 (MQ=255)
                                                               gccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgc                                  >  2:1749997/1‑101 (MQ=255)
                                                                cccATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgcg                                 >  1:2056348/1‑101 (MQ=255)
                                                                cccATCTGGATCGCCACGGCGCAGATGTCGGAGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgcg                                 >  2:3664244/1‑101 (MQ=255)
                                                                  cATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgatcg                               >  1:2964595/1‑97 (MQ=255)
                                                                   aTCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTg                              <  1:3187562/101‑1 (MQ=255)
                                                                    tCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTgc                             <  1:1369972/101‑1 (MQ=255)
                                                                    tCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTgc                             <  2:142409/101‑1 (MQ=255)
                                                                       ggATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGcgcg                          >  1:3412478/1‑101 (MQ=255)
                                                                        gATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGa                         >  1:1521017/1‑101 (MQ=255)
                                                                        gATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGa                         >  2:4219847/1‑101 (MQ=255)
                                                                           cGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGc                      >  1:2309215/1‑101 (MQ=255)
                                                                            gCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGcc                     <  1:4293941/101‑1 (MQ=255)
                                                                             ccACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGccc                    >  2:2919400/1‑101 (MQ=255)
                                                                             ccACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGccc                    <  2:4021428/101‑1 (MQ=255)
                                                                             ccACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGccc                    <  1:4330468/101‑1 (MQ=255)
                                                                               aCGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCAt                  >  1:2650967/1‑101 (MQ=255)
                                                                               aCGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCAt                  >  1:1210130/1‑101 (MQ=255)
                                                                               aCGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCAt                  >  1:1881579/1‑101 (MQ=255)
                                                                                cGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATg                 >  1:2197962/1‑101 (MQ=255)
                                                                                 ggCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATgg                >  2:1442119/1‑101 (MQ=255)
                                                                                  gcgcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGc               <  2:2899472/101‑1 (MQ=255)
                                                                                   cgcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGcc              >  2:1776406/1‑101 (MQ=255)
                                                                                      aGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgc           >  2:3511656/1‑101 (MQ=255)
                                                                                       gATGTCGGCGAGTTCCTCGCGCGTGCCGCCCTCGCGCATCATCGCGCCGATGTGAAAGCCGATGCATGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcg          <  2:3924616/101‑1 (MQ=255)
                                                                                        aTGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcgc         <  1:2681279/101‑1 (MQ=255)
                                                                                          gTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGa       >  2:2732100/1‑101 (MQ=255)
                                                                                           tCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGAt      <  1:4191446/101‑1 (MQ=255)
                                                                                               cGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAt  <  2:1007348/101‑1 (MQ=255)
                                                                                               cGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAt  <  1:325552/101‑1 (MQ=255)
                                                                                               cGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAt  <  2:1474196/101‑1 (MQ=255)
                                                                                               cGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAt  >  1:3865112/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566311‑566506

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG