Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 590,814 C→G 100% Q56E (CAA→GAA)  murI → glutamate racemase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034590,8140C→G100.0% 831.3 222Q56E (CAA→GAA) murIglutamate racemase
Reads supporting (aligned to +/- strand):  new base (111/110):  ref base (0/0):  total (111/111)

Only 100 of 230 total aligned reads displayed.

TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911
                                                                                                  |                                                                                                 
tGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 <  2:2808655/101‑1 (MQ=255)
tGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 >  2:1420677/1‑101 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACCTCTACAACCTGACCCGCCGCGGCGTCGAAc                                                                                                >  1:557906/1‑101 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                >  1:1619724/1‑101 (MQ=255)
   ccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTTCGCAGCCCCGGCGACATCTACAACCTGACCTGGCGCGGCGTCGAACgg                                                                                              >  2:2987874/1‑101 (MQ=255)
   ccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACgg                                                                                              >  1:3485925/1‑101 (MQ=255)
   ccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACgg                                                                                              <  2:2327288/101‑1 (MQ=255)
   ccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACgg                                                                                              >  1:1622906/1‑101 (MQ=255)
    cGAGGTGGCCTTTGTCTATTAAGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGATGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGc                                                                                             >  1:2540491/1‑101 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            >  1:3966584/1‑101 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            >  1:1467517/1‑101 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            >  2:3594955/1‑101 (MQ=255)
       ggTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtgt                                                                                          <  2:3037218/101‑1 (MQ=255)
        gTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtgtg                                                                                         >  1:1225485/1‑101 (MQ=255)
          ggCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTggg                                                                                       >  2:1218685/1‑101 (MQ=255)
          ggCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTggg                                                                                       <  2:2651509/101‑1 (MQ=255)
           gCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGa                                                                                      >  1:1706048/1‑101 (MQ=255)
           gCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGa                                                                                      <  1:3994464/101‑1 (MQ=255)
            ccTTTGTCTATTATGGCGACAATGCCCCTGCGCCCTACGGCGTGCGCCCCCCCGACGCCATCTACAACCTGCCCTGCCGCGGCGTCGAACGGCTGTGGGAc                                                                                     <  2:1926805/101‑1 (MQ=255)
            ccTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGAc                                                                                     <  1:4279895/101‑1 (MQ=255)
            ccTTTGTCTATTATGGCGACAATGCCCATGCCCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTgggcc                                                                                     >  1:2523675/1‑99 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACCTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACg                                                                                    <  2:2276989/101‑1 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACg                                                                                    <  1:4206937/101‑1 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACg                                                                                    >  1:4112325/1‑101 (MQ=255)
                tGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAgg                                                                                 >  2:3360813/1‑101 (MQ=255)
                 gTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAggg                                                                                >  1:2893140/1‑101 (MQ=255)
                  tCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACACCCTGACCTGCCGCGGCGTCGAACGGCTGTGGGGCGAGGGc                                                                               >  2:1071703/1‑101 (MQ=255)
                     attatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGc                                                                            <  2:4239543/101‑1 (MQ=255)
                      ttatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCg                                                                           >  2:2425952/1‑101 (MQ=255)
                          ggCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCt                                                                       >  1:2601807/1‑101 (MQ=255)
                              aCAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTg                                                                   <  1:785976/101‑1 (MQ=255)
                              aCAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTg                                                                   >  2:1277765/1‑101 (MQ=255)
                                 aTGCCCATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAtt                                                                <  1:1183100/101‑1 (MQ=255)
                                   gCCCATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCt                                                              <  2:3220478/101‑1 (MQ=255)
                                     ccATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTgg                                                            >  2:2472775/1‑101 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGc                                                           >  2:3709071/1‑101 (MQ=255)
                                       aTGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          <  1:2242298/101‑1 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        <  2:3315635/101‑1 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        <  2:4177129/101‑1 (MQ=255)
                                           gcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCa                                                      >  1:4377039/1‑101 (MQ=255)
                                            cccTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCaa                                                     <  1:3431901/101‑1 (MQ=255)
                                             ccTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    >  1:3116208/1‑101 (MQ=255)
                                             ccTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    >  1:2685525/1‑101 (MQ=255)
                                              cTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   <  1:4411792/101‑1 (MQ=255)
                                              cTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   <  2:3801026/101‑1 (MQ=255)
                                              cTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   >  1:2715904/1‑101 (MQ=255)
                                               tACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  <  2:3422674/101‑1 (MQ=255)
                                               tACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  >  1:2412057/1‑101 (MQ=255)
                                               tACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  >  1:1030631/1‑101 (MQ=255)
                                                aCGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 <  2:1619618/101‑1 (MQ=255)
                                                aCGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCCGCAACACg                                                 <  2:2601636/101‑1 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  1:181156/1‑101 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  2:2041913/1‑101 (MQ=255)
                                                         gcACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcc                                        >  1:1999364/1‑101 (MQ=255)
                                                         gcACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcc                                        <  1:3361050/101‑1 (MQ=255)
                                                          cACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTCGTGATTCTGGCCTGCAACACGGCCTccgccg                                       >  2:508205/1‑101 (MQ=255)
                                                           aCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcccc                                      >  2:4264627/1‑99 (MQ=255)
                                                           aCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCcgccgc                                      >  1:700230/1‑101 (MQ=255)
                                                            cccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCcgcg                                     <  1:4179876/101‑1 (MQ=255)
                                                               ccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggcg                                  <  1:1475183/101‑1 (MQ=255)
                                                               ccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCCGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggcg                                  >  1:2751015/1‑101 (MQ=255)
                                                                cgacgaCATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGgcgc                                 <  2:3730778/101‑1 (MQ=255)
                                                                 gacgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCt                                <  2:157810/101‑1 (MQ=255)
                                                                 gaccacATCTTCCACCCGCCCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAGTCTGGCCTGCAACACGGCCCCCGCCGCGGCGCt                                <  2:1900319/101‑1 (MQ=255)
                                                                  acgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               >  2:2918829/1‑101 (MQ=255)
                                                                  acgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               >  2:1519017/1‑101 (MQ=255)
                                                                    gacATCTACACCCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaa                             <  1:1277858/101‑1 (MQ=255)
                                                                    gacATCTACACCCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaa                             <  2:2540328/101‑1 (MQ=255)
                                                                    gacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaa                             >  1:1537237/1‑101 (MQ=255)
                                                                     acATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaaa                            >  1:214417/1‑101 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAg                          <  2:590910/101‑1 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAg                          <  1:2472932/101‑1 (MQ=255)
                                                                        tCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAgg                         <  2:3137065/101‑1 (MQ=255)
                                                                        tCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAgg                         >  2:1379099/1‑101 (MQ=255)
                                                                         cTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGa                        <  2:270206/101‑1 (MQ=255)
                                                                         cTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGa                        <  2:1416438/101‑1 (MQ=255)
                                                                         cTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGa                        <  1:2932214/101‑1 (MQ=255)
                                                                           aCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATg                      >  2:3568030/1‑101 (MQ=255)
                                                                            cAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGc                     <  2:782765/101‑1 (MQ=255)
                                                                             aaCCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCa                    >  1:676511/1‑101 (MQ=255)
                                                                              acctgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAg                   <  2:3943664/101‑1 (MQ=255)
                                                                               cctgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAgg                  <  1:2659226/101‑1 (MQ=255)
                                                                               cctgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAgg                  >  2:4345881/1‑101 (MQ=255)
                                                                                 tgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGaa                <  2:4124021/101‑1 (MQ=255)
                                                                                 tgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGAAGGaa                >  1:3220401/1‑101 (MQ=255)
                                                                                  gacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAAc               <  1:2287910/101‑1 (MQ=255)
                                                                                  gacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAAc               >  2:2708871/1‑101 (MQ=255)
                                                                                   acctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACa              <  2:793322/101‑1 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAGAGGATGCAGGAACAtt            >  2:4384716/1‑101 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            >  2:3659714/1‑101 (MQ=255)
                                                                                      tgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTg           <  1:1420784/101‑1 (MQ=255)
                                                                                        ccGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGa         >  2:1651794/1‑101 (MQ=255)
                                                                                          gcggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCGGCGGCGCTGAAAAGGATGCAGGAACATTGGATc       >  1:3089543/1‑101 (MQ=255)
                                                                                          gcggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATc       <  2:3480931/101‑1 (MQ=255)
                                                                                             gcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCg    <  1:4222098/101‑1 (MQ=255)
                                                                                             gcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCg    >  2:1130240/1‑101 (MQ=255)
                                                                                             gcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCg    >  2:199350/1‑101 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   >  2:2970159/1‑101 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   <  2:2153028/101‑1 (MQ=255)
                                                                                               gTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGaa  >  1:3567459/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG