Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 625,791 G→C 100% R533R (CGG→CGC anfA → nitrogen fixation regulatory protein AnfA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034625,7910G→C100.0% 794.4 216R533R (CGG→CGCanfAnitrogen fixation regulatory protein AnfA
Reads supporting (aligned to +/- strand):  new base (97/118):  ref base (0/0):  total (97/119)

Only 100 of 230 total aligned reads displayed.

ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888
                                                                                                  |                                                                                                  
acgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGTATCGACCCGCACCGCTa                                                                                                  >  2:4015591/1‑101 (MQ=255)
 cgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                 <  2:940683/101‑1 (MQ=255)
 cgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                 >  1:915236/1‑101 (MQ=255)
 cgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                 <  2:3394946/101‑1 (MQ=255)
 cgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                 <  2:374248/101‑1 (MQ=255)
   acCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcg                                                                                               >  1:876884/1‑101 (MQ=255)
   acCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcg                                                                                               >  2:2221801/1‑101 (MQ=255)
   acCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcg                                                                                               <  1:239515/101‑1 (MQ=255)
    cccAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcgc                                                                                              >  2:79469/1‑101 (MQ=255)
    cccAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcgc                                                                                              <  1:3383074/101‑1 (MQ=255)
     ccAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcgcg                                                                                             <  1:1415732/101‑1 (MQ=255)
     ccAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcgcg                                                                                             >  2:2674405/1‑101 (MQ=255)
       aGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCt                                                                                           >  1:435292/1‑101 (MQ=255)
        gggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCtt                                                                                          >  1:3695524/1‑101 (MQ=255)
        gggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGCATCGCGCCGGGGGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTaatccgctt                                                                                          >  2:1548551/1‑93 (MQ=255)
                 cGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                 <  2:3642049/101‑1 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                                >  2:1263228/1‑101 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                                >  2:2854728/1‑101 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                                <  1:4408416/101‑1 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                                >  1:32612/1‑101 (MQ=255)
                    ccAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGa                                                                              >  2:2896424/1‑101 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                             >  1:348553/1‑101 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                             <  1:2647987/101‑1 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                             <  2:3370863/101‑1 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                             >  1:216351/1‑101 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                             <  1:446820/101‑1 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                            >  2:1364668/1‑101 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                            >  1:4125416/1‑101 (MQ=255)
                        gcggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGc                                                                          >  2:4286727/1‑101 (MQ=255)
                         cggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGcc                                                                         >  1:203602/1‑101 (MQ=255)
                         cggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGcc                                                                         <  2:669490/101‑1 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                        >  2:515420/1‑101 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                        >  2:21960/1‑101 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                        >  1:2974993/1‑101 (MQ=255)
                             cGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATg                                                                     <  2:2540129/101‑1 (MQ=255)
                             cGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATg                                                                     <  1:244233/101‑1 (MQ=255)
                              gAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGa                                                                    >  2:2563798/1‑101 (MQ=255)
                               aGCTTTTGCAGGTCTCGCGCCGGTTGCTGGGAGTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGAc                                                                   <  2:2051440/101‑1 (MQ=255)
                               aGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGAc                                                                   <  1:1511440/101‑1 (MQ=255)
                               aGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGAc                                                                   >  1:861079/1‑101 (MQ=255)
                                 cTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCg                                                                 >  2:1352788/1‑101 (MQ=255)
                                  ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGa                                                                >  1:1247726/1‑101 (MQ=255)
                                  ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGa                                                                <  1:3049162/101‑1 (MQ=255)
                                   tttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGaa                                                               >  1:387804/1‑101 (MQ=255)
                                   tttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGaa                                                               >  1:2257507/1‑101 (MQ=255)
                                     tGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATa                                                             >  2:3092742/1‑101 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGTGGCCGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                           <  1:3804611/101‑1 (MQ=255)
                                        aGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCAGATCGACGGCCAATGACCGAATACCg                                                          >  1:3734903/1‑101 (MQ=255)
                                        aGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCAACGACCCGCACCGCTATTGCGATTCGGCCTGATCGACGGCCAATCACCGAATACCg                                                          >  1:3609786/1‑101 (MQ=255)
                                          gTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGc                                                        >  2:1228481/1‑101 (MQ=255)
                                             tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                     <  1:815904/101‑1 (MQ=255)
                                             tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                     <  2:2173360/101‑1 (MQ=255)
                                              cgcgCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCg                                                    >  1:1553765/1‑101 (MQ=255)
                                                cgcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCt                                                  <  2:1142255/101‑1 (MQ=255)
                                                 gcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCtt                                                 <  1:3078227/101‑1 (MQ=255)
                                                 gcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCtt                                                 <  1:4039614/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                                <  2:2285922/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                                <  1:4108299/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGCCATCGTCCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATCCCGGCCCCGCTTc                                                <  2:1420255/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGCGGCCGCAGGGCAGCGACCCGCCCCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                                <  2:748586/101‑1 (MQ=255)
                                                   cGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcg                                               >  2:3818694/1‑101 (MQ=255)
                                                    gggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcgg                                              >  2:3207523/1‑101 (MQ=255)
                                                     ggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggc                                             >  2:850260/1‑101 (MQ=255)
                                                     ggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggc                                             <  2:4351142/101‑1 (MQ=255)
                                                       tGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcgg                                           <  2:1895645/101‑1 (MQ=255)
                                                       tGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcgg                                           <  2:1414099/101‑1 (MQ=255)
                                                        gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                          <  2:915151/101‑1 (MQ=255)
                                                        gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                          <  1:2489012/101‑1 (MQ=255)
                                                         cTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATGGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCgggg                                         <  2:4247326/101‑1 (MQ=255)
                                                          tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggggg                                        >  1:136153/1‑100 (MQ=255)
                                                          tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGc                                        >  1:3180476/1‑101 (MQ=255)
                                                          tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGc                                        >  2:2382153/1‑101 (MQ=255)
                                                          tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGc                                        >  1:748048/1‑101 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGGTCGGCATCGGCCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGTCtt                                      <  2:2002255/101‑1 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGTCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                      <  2:3609538/101‑1 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                      <  1:1719819/101‑1 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                      <  2:2741363/101‑1 (MQ=255)
                                                                 gCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCt                                 <  2:1871339/101‑1 (MQ=255)
                                                                 gCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCt                                 <  2:348518/101‑1 (MQ=255)
                                                                  cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                                >  1:110260/1‑101 (MQ=255)
                                                                   ggATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCttt                               >  1:2332072/1‑101 (MQ=255)
                                                                   ggATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCttt                               >  1:647893/1‑101 (MQ=255)
                                                                        gggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGc                          >  2:159613/1‑101 (MQ=255)
                                                                          gcggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAg                        <  1:276747/101‑1 (MQ=255)
                                                                           cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTTTTTGCGGCAG.c                      >  2:1505263/1‑101 (MQ=255)
                                                                           cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.c                      >  1:3674547/1‑101 (MQ=255)
                                                                              cTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.GCCg                   >  2:297169/1‑101 (MQ=255)
                                                                               tCGGCATCGACCCGCACCGCTATCGCGCTTCGGGCTGATCGACGGCCAATGGCCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGcccgccgt                   >  2:3559494/1‑94 (MQ=255)
                                                                                cGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGtt                 <  2:2473649/101‑1 (MQ=255)
                                                                                 ggCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTc                <  1:1006647/101‑1 (MQ=255)
                                                                                   cATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.GCCggttgg              >  2:409196/1‑96 (MQ=255)
                                                                                   cATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.GCCGTTCgg              >  1:2599783/1‑101 (MQ=255)
                                                                                   cATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCgg              <  1:1439311/101‑1 (MQ=255)
                                                                                      cGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGCTCGGGAt           >  1:1142437/1‑101 (MQ=255)
                                                                                       gACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCGGAAtt          <  1:2858623/101‑1 (MQ=255)
                                                                                       gACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCGGAAtt          <  2:455127/101‑1 (MQ=255)
                                                                                        aCCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCGGAATTc         <  2:876799/101‑1 (MQ=255)
                                                                                         cccGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCGGAATTcc        <  2:588189/101‑1 (MQ=255)
                                                                                           cGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCCGTCCGGATTTcgt       >  1:561868/1‑99 (MQ=255)
                                                                                              aCCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCGGAATTCCGTTTa   <  2:2257362/101‑1 (MQ=255)
                                                                                                  |                                                                                                  
ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAG.CCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG