BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	66,387	T→G	100%	S518S (TCT→TCG)	flhA →	flagellar biosynthesis protein FlhA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	66,387	0	T→G	100.0%	681.5	187	S518S (TCT→TCG)	flhA	flagellar biosynthesis protein FlhA
Reads supporting (aligned to +/- strand): new base (99/88): ref base (0/0): total (99/88)

Only 100 of 196 total aligned reads displayed.


GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484


gTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGaa                                                                                                 >  2:1660474/1‑101 (MQ=255)


  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGGGTCGAAcc                                                                                               <  1:1851661/101‑1 (MQ=255)


  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAcc                                                                                               <  2:3089061/101‑1 (MQ=255)


  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAcc                                                                                               <  2:3631071/101‑1 (MQ=255)


  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAcc                                                                                               >  1:3507979/1‑101 (MQ=255)


   aTCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccg                                                                                              >  2:714011/1‑101 (MQ=255)


   aTCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccg                                                                                              >  1:1223856/1‑101 (MQ=255)


    tCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgc                                                                                             <  2:128524/101‑1 (MQ=255)


     ccGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgcc                                                                                            <  2:1043551/101‑1 (MQ=255)


        gcgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCgccgcc                                                                                         <  1:3196371/101‑1 (MQ=255)


        gcgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCgccgcc                                                                                         <  1:2011970/101‑1 (MQ=255)


        gcgcAACTCCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCgccgcc                                                                                         <  1:3295487/101‑1 (MQ=255)


         cgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCt                                                                                        >  2:2720275/1‑101 (MQ=255)


          gcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTg                                                                                       >  2:779524/1‑101 (MQ=255)


            aaCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCt                                                                                     <  2:1869719/101‑1 (MQ=255)


             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    <  1:2210554/101‑1 (MQ=255)


             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    >  1:4116189/1‑101 (MQ=255)


               ttCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGa                                                                                  <  1:1910372/101‑1 (MQ=255)


                tCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGAc                                                                                 <  2:2987529/101‑1 (MQ=255)


                 cGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACg                                                                                <  1:1301700/101‑1 (MQ=255)


                  ggCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGa                                                                               <  1:3314060/101‑1 (MQ=255)


                   gCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAg                                                                              >  1:4003515/1‑101 (MQ=255)


                     cGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCt                                                                            >  1:23/1‑101 (MQ=255)


                         tgatgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATc                                                                        >  1:3075507/1‑101 (MQ=255)


                         tgatgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATc                                                                        >  1:4437300/1‑101 (MQ=255)


                          gatgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcc                                                                       >  1:1048678/1‑101 (MQ=255)


                           atgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATccc                                                                      >  1:4395018/1‑101 (MQ=255)


                            tgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcccc                                                                     >  1:1147694/1‑101 (MQ=255)


                            tgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcccc                                                                     <  2:3944752/101‑1 (MQ=255)


                             gaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  2:2303911/101‑1 (MQ=255)


                               cGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAc                                                                  >  1:837704/1‑101 (MQ=255)


                               cGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAc                                                                  <  1:2753207/101‑1 (MQ=255)


                                    cGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGt                                                             >  2:2529307/1‑101 (MQ=255)


                                     gggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            <  1:2699122/101‑1 (MQ=255)


                                     gggCGCTGCGCCGCCTACTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            >  1:3011457/1‑101 (MQ=255)


                                     gggCGCTGCCCCCCCTGCGCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            <  2:2032741/101‑1 (MQ=255)


                                         gcTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCgg                                                        >  1:4110816/1‑101 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      <  2:2037999/101‑1 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  2:4321803/1‑101 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      <  2:3312635/101‑1 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  2:3014322/1‑101 (MQ=255)


                                           tGCCCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      <  2:3755170/101‑1 (MQ=255)


                                              gccgccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGAt                                                   <  1:1660585/101‑1 (MQ=255)


                                                 gccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctg                                                <  2:3960461/101‑1 (MQ=255)


                                                 gccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctg                                                >  1:978955/1‑101 (MQ=255)


                                                   cTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              <  1:2362668/101‑1 (MQ=255)


                                                   cTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              <  2:3428036/101‑1 (MQ=255)


                                                    tGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctg                                             <  2:915905/101‑1 (MQ=255)


                                                    tGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATACCCGACAAGGTTCCGGTCGATctgctg                                             <  1:753973/101‑1 (MQ=255)


                                                      cTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgct                                           <  1:1422207/101‑1 (MQ=255)


                                                      cTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCCCATCCCCGACAAGGTTCCCTTCGATctgctgct                                           >  2:3306578/1‑101 (MQ=255)


                                                        cGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTgg                                         <  1:619148/101‑1 (MQ=255)


                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        <  2:1274765/101‑1 (MQ=255)


                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTAGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        >  1:664943/1‑101 (MQ=255)


                                                          aGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       >  1:3432833/1‑101 (MQ=255)


                                                          aGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       <  1:2483549/101‑1 (MQ=255)


                                                             aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    >  1:4157622/3‑101 (MQ=255)


                                                             aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    >  1:374862/3‑101 (MQ=255)


                                                              agtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgc                                   >  1:3265069/2‑101 (MQ=255)


                                                                tgtgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgctg                                 >  2:2809528/1‑101 (MQ=255)


                                                                 gtgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgctgc                                >  2:421469/1‑101 (MQ=255)


                                                                 gtgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgctgc                                <  2:408001/101‑1 (MQ=255)


                                                                   gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  2:1744188/1‑101 (MQ=255)


                                                                   gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  2:2578578/1‑101 (MQ=255)


                                                                   gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  1:1401573/1‑101 (MQ=255)


                                                                   gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  2:1876262/1‑101 (MQ=255)


                                                                    cAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTc                             >  1:3171701/1‑101 (MQ=255)


                                                                     aaCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTct                            >  2:857891/1‑101 (MQ=255)


                                                                        cTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgct                         <  1:2862473/101‑1 (MQ=255)


                                                                        cTTTCCGACCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgct                         <  1:2229657/101‑1 (MQ=255)


                                                                        cTTCCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTTCTGCTGGCGGTGTTGCGTctgct                         <  2:960905/101‑1 (MQ=255)


                                                                         tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGGGTcagatc                        >  2:3531098/1‑96 (MQ=255)


                                                                         tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctg                        >  1:385258/1‑101 (MQ=255)


                                                                          ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  1:1443967/1‑101 (MQ=255)


                                                                          ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  1:2074290/1‑101 (MQ=255)


                                                                          ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  1:285570/1‑101 (MQ=255)


                                                                          ttCCGATCCCGCCCGCGCCGAGCCGGACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTCCGTatgctgc                       >  2:414532/1‑101 (MQ=255)


                                                                           tCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgct                      <  1:1058239/101‑1 (MQ=255)


                                                                            ccGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgctg                     <  1:3540264/101‑1 (MQ=255)


                                                                            ccGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgctg                     >  1:2454542/1‑101 (MQ=255)


                                                                             cGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTgg                    <  1:2569167/101‑1 (MQ=255)


                                                                             cGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTgg                    <  2:1411159/101‑1 (MQ=255)


                                                                              gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGga                   >  1:2829876/1‑101 (MQ=255)


                                                                              gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGga                   >  1:3352132/1‑101 (MQ=255)


                                                                               ctCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaa                  <  2:2213486/100‑1 (MQ=255)


                                                                               aTCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGGCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaa                  >  2:821868/1‑101 (MQ=255)


                                                                                   cgcccgcCCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAAc              <  2:1789718/101‑1 (MQ=255)


                                                                                      ccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACggg           >  1:2391233/1‑101 (MQ=255)


                                                                                       cgcgCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgt          >  2:3697312/1‑101 (MQ=255)


                                                                                        gcgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACggggg         >  2:4101146/1‑99 (MQ=255)


                                                                                        gcgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtg         <  1:2988941/101‑1 (MQ=255)


                                                                                         cgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtgt        <  1:3518895/101‑1 (MQ=255)


                                                                                          gcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTc       >  2:4338837/1‑101 (MQ=255)


                                                                                          gcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTc       >  1:4245915/1‑101 (MQ=255)


                                                                                          cccGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTc       <  1:3060309/100‑1 (MQ=255)


                                                                                            cGAGTCGACCCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGa     <  2:1200007/101‑1 (MQ=255)


                                                                                             gAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGAt    <  2:153174/101‑1 (MQ=255)


                                                                                              aGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATc   >  1:620532/1‑101 (MQ=255)


                                                                                               gTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATcc  <  1:2710313/101‑1 (MQ=255)


                                                                                               gTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATcc  <  2:4437028/101‑1 (MQ=255)


GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG