Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,483 G→A 20.4% I708I (ATC→CGA RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4830G→A20.4% 114.9 ‑3.8 ‑0.1201I708I (ATC→ATTRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (12/29):  ref base (79/80):  total (91/110)
Fisher's exact test strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.58e-01

Only 100 of 208 total aligned reads displayed.

CGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCG  >  NC_014034/681383‑681583
                                                                                                    |                                                                                                     
 ggCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.a                                                                                                     >  2:1849205/1‑101 (MQ=255)
  gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.At                                                                                                    <  1:1104107/101‑1 (MQ=255)
   ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATc                                                                                                   >  2:2433275/1‑101 (MQ=255)
   cactCGTGCCCGAGCCCAGCCCGGAGCGCGCCCTCGCCCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCCCGCCG.ATc                                                                                                   <  2:2912958/98‑1 (MQ=37)
    cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGGTCTGCGTGCTGGTCAGCGCGCCG.ATCt                                                                                                  <  2:2961614/101‑1 (MQ=255)
    cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCt                                                                                                  <  2:874939/101‑1 (MQ=255)
    cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCt                                                                                                  <  1:1578034/101‑1 (MQ=255)
     aTCGTGGCCGAGGCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                                                 >  1:2422813/1‑101 (MQ=255)
      tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgc                                                                                                <  2:3183806/101‑1 (MQ=255)
         tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGcc                                                                                             >  1:2796337/1‑101 (MQ=255)
            ccGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAg                                                                                          >  2:1198827/1‑101 (MQ=255)
            ccGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAg                                                                                          <  1:1484222/101‑1 (MQ=255)
              gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                                        >  1:206418/1‑101 (MQ=255)
              gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCCTGCTGGTCCGCGCGCCG.ATCTGCGCCGAGcc                                                                                        >  1:3056405/1‑101 (MQ=255)
               agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGccc                                                                                       >  2:1151173/1‑101 (MQ=255)
               agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGccc                                                                                       >  2:4395246/1‑101 (MQ=255)
                    agcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAcg                                                                                  <  1:4397344/101‑1 (MQ=255)
                      cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAcgcg                                                                                <  2:2830636/101‑1 (MQ=255)
                       ccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACgcgc                                                                               >  1:2029343/1‑101 (MQ=255)
                           agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCgccgcc                                                                           <  1:1329364/101‑1 (MQ=255)
                            gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCt                                                                          <  1:3746190/101‑1 (MQ=255)
                            gagCGCCGGCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCt                                                                          <  1:197067/101‑1 (MQ=255)
                              gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTgc                                                                        <  1:477497/101‑1 (MQ=255)
                                gcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTgcgc                                                                      >  2:227220/1‑101 (MQ=255)
                                gcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTgcgc                                                                      <  2:219195/101‑1 (MQ=255)
                                 ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                                                     >  2:3057984/1‑101 (MQ=255)
                                 ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                                                     >  1:1376502/1‑101 (MQ=255)
                                  cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                    >  1:1664745/1‑101 (MQ=255)
                                  cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                    >  1:2873829/1‑101 (MQ=255)
                                   gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgc                                                                   >  2:2207230/1‑100 (MQ=255)
                                     cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                                                 >  1:1057054/1‑101 (MQ=255)
                                     cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                                                 >  1:1595208/1‑101 (MQ=255)
                                      gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCACAGACGCGCCGCCTGCGCcgtcgt                                                                >  1:1750279/1‑101 (MQ=255)
                                       cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                               <  1:2182652/101‑1 (MQ=255)
                                       cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                               <  2:2726811/101‑1 (MQ=255)
                                        gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                              >  2:2484861/1‑101 (MQ=255)
                                        gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                              <  1:1386350/101‑1 (MQ=255)
                                         gggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAg                                                             <  1:97186/91‑1 (MQ=2)
                                          ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                            <  1:738253/92‑1 (MQ=2)
                                          ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                            <  2:4043686/92‑1 (MQ=2)
                                           gagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgcg                                                           <  2:1592623/93‑1 (MQ=2)
                                           gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                           <  2:3263145/101‑1 (MQ=255)
                                           gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                           >  2:1361671/1‑101 (MQ=255)
                                           gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                           <  1:2598551/101‑1 (MQ=255)
                                           gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                           >  1:68898/1‑101 (MQ=255)
                                           gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                           <  2:2636672/101‑1 (MQ=255)
                                             tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                                         <  1:2002385/101‑1 (MQ=255)
                                              ccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGccc                                                        >  2:3550824/6‑100 (MQ=2)
                                               tGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                       <  2:14506/101‑1 (MQ=255)
                                                gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                      >  1:2293151/1‑101 (MQ=255)
                                                gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                      <  1:3196885/98‑1 (MQ=2)
                                                 cgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGa                                                     >  2:826252/1‑101 (MQ=255)
                                                 caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                                                     >  2:1485320/3‑101 (MQ=2)
                                                  gcCAGATCCCCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                    <  2:3447877/101‑1 (MQ=255)
                                                  accAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                    <  2:704030/100‑1 (MQ=9)
                                                   ccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                   <  1:2497133/101‑1 (MQ=2)
                                                    cAGATCCGCCGCCTAGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                                  <  1:205131/101‑1 (MQ=255)
                                                     aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                 >  1:578663/1‑101 (MQ=255)
                                                       aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                               <  1:2238568/101‑1 (MQ=255)
                                                        tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCACCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                              <  2:1056966/101‑1 (MQ=255)
                                                         ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                             <  1:1628757/101‑1 (MQ=255)
                                                           gcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                           <  1:3699636/92‑1 (MQ=9)
                                                           gcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                           >  1:3979609/10‑101 (MQ=9)
                                                           gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                           >  2:4058028/1‑101 (MQ=255)
                                                             ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                         <  1:3033885/94‑1 (MQ=9)
                                                              gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        <  1:1151264/101‑1 (MQ=255)
                                                              gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        <  2:2042354/101‑1 (MQ=255)
                                                               tttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccg                                       >  1:263843/6‑100 (MQ=9)
                                                                 tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                     <  2:4280718/101‑1 (MQ=255)
                                                                  cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                    <  1:780467/101‑1 (MQ=255)
                                                                   gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                   >  2:1917211/1‑101 (MQ=255)
                                                                   caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                   <  2:3630541/100‑6 (MQ=9)
                                                                   caGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                   >  2:1630652/2‑96 (MQ=2)
                                                                    aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                  >  2:2289594/1‑101 (MQ=255)
                                                                    aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                  >  1:3504570/1‑101 (MQ=2)
                                                                    aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                  >  2:221648/1‑101 (MQ=2)
                                                                     gcgcCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcg                                 <  1:3122797/101‑1 (MQ=255)
                                                                      cgccgcAACCTGCGTGCTGGACAGCGCCAGC.AGCTGCGTCGAGTCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGGTGTCATCGGTGCCGAGGccct                                <  1:307241/101‑3 (MQ=9)
                                                                       gccgcGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                               >  2:1293045/1‑101 (MQ=255)
                                                                       gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                               <  2:798942/101‑4 (MQ=9)
                                                                       gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                               <  2:3937978/101‑4 (MQ=2)
                                                                         cgcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                             >  2:4303367/1‑101 (MQ=255)
                                                                         cgcgATCTGCGTGCGGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                             <  2:2525780/101‑1 (MQ=255)
                                                                          gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                            <  1:1917335/101‑1 (MQ=255)
                                                                          gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                            <  1:4334650/101‑1 (MQ=255)
                                                                            gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                          <  1:3220519/101‑1 (MQ=255)
                                                                             aTCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                         >  1:4219645/1‑101 (MQ=255)
                                                                                   gTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGccgccgg                   >  2:2128132/1‑100 (MQ=255)
                                                                                   gTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                   >  2:2308907/1‑101 (MQ=255)
                                                                                    tGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                  >  2:4161584/1‑101 (MQ=255)
                                                                                     gCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                 >  2:3647900/1‑101 (MQ=255)
                                                                                        ggTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa              <  2:3574863/101‑1 (MQ=255)
                                                                                         atctcggtgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg             >  2:2030951/9‑101 (MQ=255)
                                                                                          tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc            <  1:4161094/101‑1 (MQ=255)
                                                                                          tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc            <  1:685771/101‑1 (MQ=255)
                                                                                          tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc            <  1:2101243/101‑1 (MQ=255)
                                                                                                cgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccccc      >  2:636821/1‑101 (MQ=255)
                                                                                                 gcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa     <  2:1567954/101‑1 (MQ=255)
                                                                                                  ccG.ATCTGCCCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAc    <  1:826334/101‑1 (MQ=255)
                                                                                                   cG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc   >  1:1226624/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCG  >  NC_014034/681383‑681583

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG